A bioprinted complex tissue model for myotendinous junction with biochemical and biophysical cues

Bioengineering & Translational Medicine, Journal Year: 2022, Volume and Issue: 7(3)

Published: April 4, 2022

In the musculoskeletal system, myotendinous junction (MTJ) is optimally designed from aspect of force transmission generated a muscle through tendon onto bone to induce movement. Although MTJ key complex tissue in transmission, realistic fabrication, and formation tissues can be limited. To obtain construct, we prepared two bioinks, muscle- tendon-derived decellularized extracellular matrix (dECM), which myogenic tenogenic differentiation human adipose-derived stem cells (hASCs). By using modified bioprinting process supplemented with nozzle consisting single-core channel double-sheath channels, achieve three different types units, composed muscle, tendon, interface zones. Our results indicated that bioprinted dECM-based constructs induced hASCs differentiation. addition, significantly higher MTJ-associated gene expression was detected at cell-mixing zone than other models. Based on results, model potential platform for understanding interaction between cells, even method extensively applied tissues.

Language: Английский

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Regulation of Satellite Cells Functions during Skeletal Muscle Regeneration: A Critical Step in Physiological and Pathological Conditions

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 25(1), P. 512 - 512

Published: Dec. 29, 2023

Skeletal muscle regeneration is a complex process involving the generation of new myofibers after trauma, competitive physical activity, or disease. In this context, adult skeletal stem cells, also known as satellite cells (SCs), play crucial role in regulating tissue homeostasis and activating regeneration. Alterations their number function have been associated with various pathological conditions. The main factors involved dysregulation SCs' activity are inflammation, oxidative stress, fibrosis. This review critically summarizes current knowledge on SCs It examines changes three most common severe disorders: sarcopenia, muscular dystrophy, cancer cachexia. Understanding molecular mechanisms dysregulations essential for improving treatments, such exercise, developing personalized approaches to reactivate SCs.

Language: Английский

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A single cell spatial temporal atlas of skeletal muscle reveals cellular neighborhoods that orchestrate regeneration and become disrupted in aging

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: June 10, 2022

Abstract Our mobility requires muscle regeneration throughout life. Yet our knowledge of the interplay cell types required to rebuild injured is lacking, because most single assays require tissue dissociation. Here we use multiplexed spatial proteomics and neural network analyses resolve a spatiotemporal atlas 34 during aging. This maps interactions immune, fibrogenic, vascular, nerve, myogenic cells at sites injury in relation architecture extracellular matrix. Spatial pseudotime mapping reveals sequential cellular neighborhoods that mediate repair nodal role for immune cells. We confirm this by macrophage depletion, which triggers formation aberrant obstruct repair. In aging, dysregulation chronic, are disrupted, an autoimmune response evident denervation. findings highlight ecosystem orchestrates regeneration, altered Highlights Single resolution resolves multicellular efficient skeletal Highly proteomics, machine learning uncovers temporal dynamics crosstalk between stem myofibers coherent efficacious coordinating aged muscle, disrupted chronically inflamed state autoimmunity

Language: Английский

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Identification of porcine adipose progenitor cells by fluorescence-activated cell sorting for the preparation of cultured fat by 3D bioprinting

Food Research International, Journal Year: 2022, Volume and Issue: 162, P. 111952 - 111952

Published: Sept. 19, 2022

Language: Английский

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Inflammatory, mitochondrial, and senescence-related markers: Underlying biological pathways of muscle aging and new therapeutic targets

Experimental Gerontology, Journal Year: 2023, Volume and Issue: 178, P. 112204 - 112204

Published: May 18, 2023

The maintenance of functional health is pivotal for achieving independent life in older age. aged muscle characterized by ultrastructural changes, including loss type I and II myofibers a greater proportion cytochrome c oxidase deficient succinate dehydrogenase positive fibers. Both intrinsic (e.g., altered proteostasis, DNA damage, mitochondrial dysfunction) extrinsic factors denervation, metabolic regulation, declines satellite cells, inflammation) contribute to aging. Being hub several cellular activities, mitochondria are key myocyte viability dysfunction has been implicated age-associated physical decline. organelles via quality control (MQC) processes is, therefore, crucial skeletal myofiber organismal health. autophagy-lysosome pathway emerged as critical step MQC disposing proteins their tagging autophagosome incorporation delivery the lysosome clearance. This was found be physically inactive adults. A relationship between this tissue composition lower extremities well performance also identified. Therefore, integrating structure measures evaluation may promising strategy devising interventions fostering

Language: Английский

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