Chemical Constituents of Euphorbia stracheyi Boiss (Euphorbiaceae) DOI Creative Commons
Hui Zhu,

Xiangxiang Ren,

Yanbo Huang

et al.

Metabolites, Journal Year: 2023, Volume and Issue: 13(7), P. 852 - 852

Published: July 15, 2023

Euphorbia stracheyi Boiss was used for hemostasis, analgesia, and muscular regeneration in traditional Chinese medicine. To study the chemical constituents of E. stracheyi, ethyl acetate part methanol extract whole plant separated by silica gel, sephadex LH-20 column chromatography, semi-preparative HPLC. The isolation led to characterization a new lathyrane type diterpenoid, euphostrachenol A (1), as well eleven known compounds (2-11), including lathyrane, three ingenane-type two abietane-type diterpenoids, ionones, flavonoids. structures these were established using 1D- 2D-NMR experiments, mass spectrometry, X-ray crystallographic experiments. MTT method determine cytotoxic activity five cancer cell lines (Leukemia HL-60, lung A-549, liver SMMC-7721, breast MCF-7, colon SW480) on isolated compounds. However, only compound 4 showed moderate cytotoxicity against lines, with IC50 values ranging from 10.28 29.70 μM, while others inactive. Our investigation also confirmed absence jatrophane-type diterpenoids species, which may be related its special habitat.

Language: Английский

Transcriptional regulatory network of high-value active ingredients in medicinal plants DOI
Han Zheng, Xueqing Fu, Jin Shao

et al.

Trends in Plant Science, Journal Year: 2023, Volume and Issue: 28(4), P. 429 - 446

Published: Jan. 6, 2023

Language: Английский

Citations

97
Mitochondrial Genome Sequence of Salvia officinalis (Lamiales: Lamiaceae) Suggests Diverse Genome Structures in Cogeneric Species and Finds the Stop Gain of Genes through RNA Editing Events DOI Open Access
Heyu Yang, Haimei Chen, Yang Ni

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(6), P. 5372 - 5372

Published: March 11, 2023

Our previous study was the first to confirm that predominant conformation of mitochondrial genome (mitogenome) sequence Salvia species contains two circular chromosomes. To further understand organization, variation, and evolution mitogenomes, we characterized mitogenome officinalis. The S. officinalis sequenced using Illumina short reads Nanopore long assembled a hybrid assembly strategy. We found also had chromosomes were 268,341 bp (MC1) 39,827 (MC2) in length. encoded an angiosperm-typical set 24 core genes, 9 variable 3 rRNA 16 tRNA genes. many rearrangements through inter- intra-specific comparisons. A phylogenetic analysis coding sequences (CDs) 26 common protein-coding genes (PCGs) 11 Lamiales 2 outgroup taxa strongly indicated sister taxon miltiorrhiza, consistent with results obtained concatenated CDs plastid mapping RNA-seq data PCGs led identification 451 C-to-U RNA editing sites from 31 mitogenome. Using PCR amplification Sanger sequencing methods, successfully validated 113 126 PCGs. this suggest are chromosomes, stop gain rpl5 events

Language: Английский

Citations

29
Chromosome-level genome assembly of Salvia miltiorrhiza with orange roots uncovers the role of Sm2OGD3 in catalyzing 15,16-dehydrogenation of tanshinones DOI Creative Commons
Xian Pan,

Yujie Chang,

Caili Li

et al.

Horticulture Research, Journal Year: 2023, Volume and Issue: 10(6)

Published: April 13, 2023

Abstract Salvia miltiorrhiza is well known for its clinical practice in treating heart and cardiovascular diseases. Its roots, used traditional Chinese medicine materials, are usually brick-red due to accumulation of red pigments, such as tanshinone IIA I. Here we report a S. line (shh) with orange roots. Compared the roots normal plants, contents tanshinones single bond at C-15,16 were increased, whereas those double significantly decreased shh. We assembled high-quality chromosome-level genome Phylogenomic analysis showed that relationship between two lines was closer than It indicates shh could not be mutant an extant Comparative genomic transcriptomic analyses 1.0 kb DNA fragment deleted Sm2OGD3m. Complementation assay overexpression intact Sm2OGD3 hairy recovered furan D-ring accumulation. Consistently, vitro protein catalyzed conversion cyptotanshinone, 15,16-dihydrotanshinone I 1,2,15,16-tetrahydrotanshinone into IIA, 1,2-dihydrotanshinone I, respectively. Thus, functions 15,16-dehydrogenase key enzyme biosynthesis. The results provide novel insights metabolic network medicinally important compounds.

Language: Английский

Citations

27
The genomes of medicinal skullcaps reveal the polyphyletic origins of clerodane diterpene biosynthesis in the family Lamiaceae DOI Creative Commons
Haixiu Li, Song Wu, Ruoxi Lin

et al.

Molecular Plant, Journal Year: 2023, Volume and Issue: 16(3), P. 549 - 570

Published: Jan. 13, 2023

The presence of anticancer clerodane diterpenoids is a chemotaxonomic marker for the traditional Chinese medicinal plant Scutellaria barbata, although molecular mechanisms behind biosynthesis are unknown. Here, we report high-quality assembly 414.98 Mb genome S. barbata into 13 pseudochromosomes. Using phylogenomic and biochemical data, mapped plastidial metabolism kaurene (gibberellins), abietane, diterpenes in three species family Lamiaceae (Scutellaria baicalensis, Salvia splendens), facilitating identification genes involved clerodanes, kolavenol, isokolavenol. We show that evolved through recruitment neofunctionalization from gibberellin abietane metabolism. Despite assumed monophyletic origin biosynthesis, which widespread Lamiaceae, our data distinct evolutionary lineages suggest polyphyletic origins Lamiaceae. Our study not only provides significant insights evolution biosynthetic pathways mint family, but also will facilitate production clerodanes future metabolic engineering efforts.

Language: Английский

Citations

25
A chromosome-level genome assembly reveals that a bipartite gene cluster formed via an inverted duplication controls monoterpenoid biosynthesis in Schizonepeta tenuifolia DOI Creative Commons
Chanchan Liu, Samuel J. Smit,

Jingjie Dang

et al.

Molecular Plant, Journal Year: 2023, Volume and Issue: 16(3), P. 533 - 548

Published: Jan. 6, 2023

Biosynthetic gene clusters (BGCs) are regions of a genome where genes involved in biosynthetic pathway proximity. The origin and evolution plant BGCs as well their role specialized metabolism remain largely unclear. In this study, we have assembled chromosome-scale Japanese catnip (Schizonepeta tenuifolia) discovered BGC that contains multiple copies four adjacent steps the biosynthesis p-menthane monoterpenoids. This has an unprecedented bipartite structure, with mirrored separated by 260 kilobases. includes identical encoding old yellow enzyme, type flavin-dependent reductase. vitro assays virus-induced silencing revealed encodes missing isopiperitenone enzyme evolved from completely different family to reductase closely related Mentha spp., indicating convergent step. Phylogenomic analysis emerged uniquely S. tenuifolia lineage through insertion into region rich monoterpene synthases. cluster gained its structure via inverted duplication. for similarities recently described duplicated pairs longifolia genome, providing example order. work expands our understanding respect both form evolution, highlights power discovery pathways.

Language: Английский

Citations

23
High‐quality chromosome‐level genome assembly and multi‐omics analysis of rosemary (Salvia rosmarinus) reveals new insights into the environmental and genome adaptation DOI Creative Commons
Yong Lai,

Jinghua Ma,

Xuebin Zhang

et al.

Plant Biotechnology Journal, Journal Year: 2024, Volume and Issue: 22(7), P. 1833 - 1847

Published: Feb. 16, 2024

Summary High‐quality genome of rosemary ( Salvia rosmarinus ) represents a valuable resource and tool for understanding evolution environmental adaptation as well its genetic improvement. However, the existing did not provide insights into relationship between antioxidant components adaptability. In this study, by employing Nanopore sequencing Hi‐C technologies, total 1.17 Gb (97.96%) sequences were mapped to 12 chromosomes with 46 121 protein‐coding genes 1265 non‐coding RNA genes. Comparative analysis reveals that had closely splendens miltiorrhiza , it diverged from them approximately 33.7 million years ago (MYA), one whole‐genome duplication occurred around 28.3 MYA in genome. Among all identified genes, 1918 gene families expanded, 35 which are involved biosynthesis components. These expanded enhance ability adverse environments. Multi‐omics (integrated transcriptome metabolome) showed tissue‐specific distribution related adaptation. During drought, heat salt stress treatments, 36 pathways carnosic acid, rosmarinic acid flavonoids up‐regulated, illustrating important role these responding abiotic stresses adjusting ROS homeostasis. Moreover, cooperating photosynthesis, substance energy metabolism, protein ion balance, collaborative system maintained cell stability improved against harsh environment. This study provides genomic data platform discovery precision breeding rosemary. Our results also new adaptive contribution resistance

Language: Английский

Citations

14
A haplotype-resolved gap-free genome assembly provides novel insight into monoterpenoid diversification in Mentha suaveolens ‘Variegata’ DOI Creative Commons
Hanting Yang, Can Wang, Guanru Zhou

et al.

Horticulture Research, Journal Year: 2024, Volume and Issue: 11(3)

Published: Jan. 17, 2024

Abstract Mentha is a commonly used spice worldwide, which possesses medicinal properties and fragrance. These characteristics are conferred, at least partially, by essential oils such as menthol. In this study, gap-free assembly with genome size of 414.3 Mb 31,251 coding genes was obtained for suaveolens ‘Variegata’. Based on its high heterozygosity (1.5%), two complete haplotypic assemblies were resolved, sizes 401.9 405.7 Mb, respectively. The telomeres centromeres each haplotype almost fully annotated. addition, we detected total 41,135 structural variations. Enrichment analysis demonstrated that involved in terpenoid biosynthesis affected these Analysis volatile metabolites showed M. mainly produces piperitenone oxide rather than We identified three the encode isopiperitenone reductase (ISPR), key rate-limiting enzyme menthol biosynthesis. However, transcription levels ISPR low. Given other expressed, ISPRs may account accumulation species. findings study provide valuable resource improving detection rate accuracy genetic variants, thereby enhancing our understanding their impact gene function expression. Moreover, haplotype-resolved offers novel insights into molecular marker-assisted breeding Mentha.

Language: Английский

Citations

12
Unraveling the specialized metabolic pathways in medicinal plant genomes: a review DOI Creative Commons
Mingcheng Wang, Shuqiao Zhang, Rui Li

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 24, 2024

Medicinal plants are important sources of bioactive specialized metabolites with significant therapeutic potential. Advances in multi-omics have accelerated the understanding metabolite biosynthesis and regulation. Genomics, transcriptomics, proteomics, metabolomics each contributed new insights into biosynthetic gene clusters (BGCs), metabolic pathways, stress responses. However, single-omics approaches often fail to fully address these complex processes. Integrated provides a holistic perspective on key regulatory networks. High-throughput sequencing emerging technologies like single-cell spatial omics deepened our cell-specific spatially resolved dynamics. Despite advancements, challenges remain managing large datasets, standardizing protocols, accounting for dynamic nature metabolism, effectively applying synthetic biology sustainable production. This review highlights recent progress omics-based research medicinal plants, discusses available bioinformatics tools, explores future trends aimed at leveraging integrated improve quality utilization plant resources.

Language: Английский

Citations

9
Chromosome-level genome assembly of Salvia sclarea DOI Creative Commons

Sang-Haeng Choi,

Yuna Kang,

Changsoo Kim

et al.

Scientific Data, Journal Year: 2025, Volume and Issue: 12(1)

Published: Jan. 4, 2025

Salvia sclarea is a medicinal herb from the Lamiaceae family, valued for its essential oil which contains sclareol, linalool, linalyl acetate, and other compounds. Despite extensive use, genetic mechanisms of S. are not well understood. This study presents chromosome-level genome assembly using Oxford Nanopore Technology, Illumina short reads, Pore-C technology. The assembled spans 499.03 Mbp with scaffold N50 50.5 Mbp, forming 11 pseudochromosomes. was validated by BUSCO analysis, indicated high completeness marked at 98.7%. 68.73% repetitive sequences, long terminal repeats (LTRs) accounting 33.01%. A total 17,202 protein-coding genes were predicted. Among them, 16,846 annotated in NCBI NR database, highest among all databases, covering 97.93% predicted genes. phylogenetic analysis revealed that shares close evolutionary relationship officinalis within genus, while species genus have evolved independently family. high-resolution offers fresh insights into biosynthesis oils aromatic compounds sclarea, establishing basis preservation.

Language: Английский

Citations

1
A long road ahead to reliable and complete medicinal plant genomes DOI Creative Commons

L. Cheng,

Zilong Wang, Qian‐Hao Zhu

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: March 4, 2025

Language: Английский

Citations

1