Heterogeneous presynaptic receptive fields contribute to directional tuning in starburst amacrine cells DOI Creative Commons

John A. Gaynes,

Samuel A. Budoff,

Michael J. Grybko

et al.

eLife, Journal Year: 2023, Volume and Issue: 12

Published: Dec. 27, 2023

The processing of visual information by retinal starburst amacrine cells (SACs) involves transforming excitatory input from bipolar (BCs) into directional calcium output. While previous studies have suggested that an asymmetry in the kinetic properties BCs along soma-dendritic axes postsynaptic cell could enhance tuning at level individual branches, it remains unclear whether biologically relevant presynaptic kinetics contribute to direction selectivity (DS) when stimulation engages entire dendritic tree. To address this question, we built multicompartmental models bipolar–SAC circuit and trained them boost tuning. We report despite significant crosstalk dissimilar preferences dendrites occur during whole-cell stimulation, rules guide BC leading optimal DS are similar single-dendrite condition. correlate model predictions empirical findings, utilized two-photon glutamate imaging study dynamics release onto ON- OFF-starburst murine retina. reveal diverse response motion both populations; algorithms on experimental data differences temporal likely correspond heterogeneous receptive field among different types, including spatial extent center surround components. In addition, demonstrate architecture composed units with experimentally recorded drive but not levels replicate suggesting other mechanisms required explain SAC function. Our provides new insights complex underlying highlights potential contribution computation SACs.

Language: Английский

Retinal origin of orientation but not direction selective maps in the superior colliculus DOI Creative Commons
Daniel de Malmazet, Norma K. Kühn, Chen Li

et al.

Current Biology, Journal Year: 2024, Volume and Issue: 34(6), P. 1222 - 1233.e7

Published: Feb. 27, 2024

Language: Английский

Citations

6
Distributed feature representations of natural stimuli across parallel retinal pathways DOI Creative Commons
Jen-Chun Hsiang, Ning Shen, Florentina Soto

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: March 1, 2024

Abstract How sensory systems extract salient features from natural environments and organize them across neural pathways is unclear. Combining single-cell population two-photon calcium imaging in mice, we discover that retinal ON bipolar cells (second-order neurons of the visual system) are divided into two blocks four types. The distribute temporal spatial information encoding, respectively. cell axons co-stratify within each block, but separate laminarly between (upper block: diverse temporal, uniform tuning; lower spatial, tuning). similarly artificial naturalistic stimuli. In addition, they differ sensitivity to coherent motion movies. Motion distributed upper blocks, multiplexed with contrast, independent scenes. Comparing responses different boutons same arbor, find all types function as computational units. Thus, our results provide insights feature extraction stimuli reveal how structural functional organization cooperate generate parallel for mammalian retina.

Language: Английский

Citations

4
A spherical code of retinal orientation selectivity enables decoding in ensembled and retinotopic operation DOI Creative Commons
Dimitrios Daniel Laniado, Yariv Maron,

John A. Gemmer

et al.

Cell Reports, Journal Year: 2025, Volume and Issue: 44(3), P. 115373 - 115373

Published: March 1, 2025

Language: Английский

Citations

0
Molecular identification of wide-field amacrine cells in mouse retina that encode stimulus orientation DOI Open Access

Silvia J. Park,

Wanyu Lei,

John J. Pisano

et al.

Published: Feb. 26, 2024

Visual information processing is sculpted by a diverse group of inhibitory interneurons in the retina called amacrine cells. Yet, for most >60 cell types, molecular identities and specialized functional attributes remain elusive. Here, we developed an intersectional genetic strategy to target wide-field cells (WACs) mouse that co-express transcription factor Bhlhe22 Kappa Opioid Receptor (KOR; B/K WACs). WACs feature straight, unbranched dendrites spanning over 0.5 mm (∼15° visual angle) produce non-spiking responses either light increments or decrements. Two-photon dendritic population imaging reveals Ca 2+ signals tuned physical orientations WAC dendrites, signifying robust structure-function alignment. establish divergent connections with multiple retinal neurons, including unexpected non-orientation-tuned ganglion bipolar Our work sets stage future comprehensive investigations enigmatic neurons: WACs.

Language: Английский

Citations

2
Neural Circuits Underlying Multifeature Extraction in the Retina DOI

Prathyusha Ravi Chander,

Laura Hanson, Pavitra Chundekkad

et al.

Journal of Neuroscience, Journal Year: 2023, Volume and Issue: 44(10), P. e0910232023 - e0910232023

Published: Nov. 13, 2023

Classic ON-OFF direction-selective ganglion cells (DSGCs) that encode the four cardinal directions were recently shown to also be orientation-selective. To clarify mechanisms underlying orientation selectivity, we employed a variety of electrophysiological, optogenetic, and gene knock-out strategies test relative contributions glutamate, GABA, acetylcholine (ACh) input are known drive DSGCs, in male female mouse retinas. Extracellular spike recordings revealed DSGCs respond preferentially either vertical or horizontal bars, those perpendicular their preferred-null motion axes. By contrast, glutamate all DSGC types measured using whole-cell patch-clamp techniques was found tuned along axis. Tuned glutamatergic excitation heavily reliant on type 5A bipolar cells, which appear electrically coupled via connexin 36 containing gap junctions vertically oriented processes wide-field amacrine cells. Vertically inputs transformed by GABAergic/cholinergic "starburst" (SACs), critical components circuit, into distinct patterns inhibition excitation. Feed-forward SAC appears "veto" preferred dorsal/ventral (but not nasal/temporal) coding "flipping" tuning 90° accounts for apparent mismatch between DSGC's spiking response. Together, these results reveal how two synaptic motifs interact generate complex feature shedding light intricate circuitry underlies visual processing retina.

Language: Английский

Citations

6
Retinal Connectomics: A Review DOI
Crystal Sigulinsky, Rebecca L. Pfeiffer, Bryan W. Jones

et al.

Annual Review of Vision Science, Journal Year: 2024, Volume and Issue: 10(1), P. 263 - 291

Published: Sept. 15, 2024

The retina is an ideal model for understanding the fundamental rules how neural networks are constructed. compact of perform all initial processing visual information before transmission to higher centers in brain. field retinal connectomics uses high-resolution electron microscopy datasets map intricate organization these and further our computations performed by revealing topologies allowable behind computations. In this article, we review some notable advances that has provided specific cells their connectivities within retina, as well shaped development break down disease. Using anatomical maps inform modeling been, will continue be, instrumental processes signals.

Language: Английский

Citations

2
Molecular identification of wide-field amacrine cells in mouse retina that encode stimulus orientation DOI Open Access

Silvia J. Park,

Wanyu Lei,

John J. Pisano

et al.

Published: Feb. 26, 2024

Visual information processing is sculpted by a diverse group of inhibitory interneurons in the retina called amacrine cells. Yet, for most >60 cell types, molecular identities and specialized functional attributes remain elusive. Here, we developed an intersectional genetic strategy to target wide-field cells (WACs) mouse that co-express transcription factor Bhlhe22 Kappa Opioid Receptor (KOR; B/K WACs). WACs feature straight, unbranched dendrites spanning over 0.5 mm (∼15° visual angle) produce non-spiking responses either light increments or decrements. Two-photon dendritic population imaging reveals Ca 2+ signals tuned physical orientations WAC dendrites, signifying robust structure-function alignment. establish divergent connections with multiple retinal neurons, including unexpected non-orientation-tuned ganglion bipolar Our work sets stage future comprehensive investigations enigmatic neurons: WACs.

Language: Английский

Citations

1
Compartmentalized pooling generates orientation selectivity in wide-field amacrine cells DOI Creative Commons

Wanyu Lei,

Damon A. Clark, Jonathan B. Demb

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(49)

Published: Nov. 27, 2024

Orientation is one of the most salient features in visual scenes. Neurons at multiple levels system detect orientation, but many cases, underlying biophysical mechanisms remain unresolved. Here, we studied for orientation detection earliest stage system, B/K wide-field amacrine cells (B/K WACs), a group giant, nonspiking interneurons mouse retina that coexpress Bhlhe22 (B) and Kappa Opioid Receptor (K). WACs exhibit orientation-tuned calcium signals along their long, straight, unbranching dendrites, which contain both synaptic inputs outputs. Simultaneous dendritic imaging somatic voltage recordings reveal individual dendrites are electrotonically isolated, exhibiting spatially confined yet extended receptive field dendrite, term “compartmentalized pooling.” Further, WAC dendrite exhibits center-surround antagonism. Phenomenological models demonstrate compartmentalized pooling generates selectivity, antagonism shapes band-pass spatial frequency tuning. At microcircuit level, receive excitation driven by contrast polarity (e.g., “ON”) glycinergic inhibition opposite “OFF”). However, this “crossover” not essential generating selectivity. A minimal model reproduced from feedforward excitatory combined with substantial increase specific membrane resistance between compartments. Collectively, our results mechanism selectivity WACs, enriching understanding diverse strategies employed throughout to orientation.

Language: Английский

Citations

1
Layer-specific anatomical and physiological features of the retina’s neurovascular unit DOI Creative Commons
William N. Grimes, David M. Berson, Adit Sabnis

et al.

Current Biology, Journal Year: 2024, Volume and Issue: 35(1), P. 109 - 120.e4

Published: Dec. 16, 2024

Language: Английский

Citations

1
New insights into retinal circuits through EM connectomics: what we have learnt and what remains to be learned DOI Creative Commons

Abhilash Sawant,

Aindrila Saha,

Jacob Khoussine

et al.

Frontiers in Ophthalmology, Journal Year: 2023, Volume and Issue: 3

Published: April 20, 2023

The retinal neural circuit is intricately wired for efficient processing of visual signals. This well-supported by the specialized connections between neurons at both functional and ultrastructural levels. Through 3D electron microscopic (EM) reconstructions circuits we have learnt much about specificities within layers including new insights into how establish perform sophisticated computations. mini-review will summarize circuitry provide details novel EM connectomics has brought our understanding circuitry. We also discuss unresolved questions that can be addressed in future.

Language: Английский

Citations

3