Cell Host & Microbe, Journal Year: 2024, Volume and Issue: 32(11), P. 1873 - 1875
Published: Nov. 1, 2024
Language: Английский
Current Opinion in Microbiology, Journal Year: 2024, Volume and Issue: 77, P. 102422 - 102422
Published: Jan. 11, 2024
Language: Английский
Citations
9
Biomolecules, Journal Year: 2024, Volume and Issue: 14(2), P. 240 - 240
Published: Feb. 18, 2024
The vaginal epithelial barrier, which integrates mechanical, immune, chemical, and microbial defenses, is pivotal in safeguarding against external pathogens upholding the microecological equilibrium. Although widely used metronidazole effectively curtails Gardnerella vaginalis, a key pathogen bacterial vaginosis, it falls short restoring barrier or reducing recurrence rates. Our prior research highlighted Lactobacillus crispatus CCFM1339, vaginally derived strain, for its capacity to modulate barrier. In cellular models, L. CCFM1339 fortified integrity of monolayer, augmented migration, facilitated repair. Remarkably, animal substantially abated secretion disruption biomarker E-cadherin (from 101.45 82.90 pg/mL) increased anti-inflammatory cytokine IL-10 (35.18% vs. model), consequently mitigating inflammation mice. Immunological assays tissues elucidated secretory IgA levels 405.56 740.62 ng/mL) curtailed IL-17 gene expression. Moreover, enhanced Lactobacilli abundance attenuated Enterobacterium Enterococcus within microbiome, underscoring potential probiotic applications regulation.
Language: Английский
Citations
6
Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)
Published: Nov. 16, 2023
The vaginal microenvironment is key in mediating susceptibility to sexually transmitted infections. A polymicrobial environment with reduced Lactobacilllus spp. characteristic of dysbiosis, associated increased production several short chain fatty acids (SCFAs), inflammation and an risk HIV-1 acquisition. In contrast, a eubiotic microbiome (VMB), dominated by Lactobacillus correlates lactic acid (LA), acidic milieu protection against HIV-1. Vaginal metabolites, specifically LA SCFAs including butyric, succinic acetic are modulation risk. We assessed the impact combined individual on epithelial cells (VK2) grown air-liquid interface cultures. Treatment VK2 SCFA + mixture showed barrier integrity, FITC dextran leakage enhanced expression cell-cell adhesion proteins. dysbiotic diminished NFκB activation inflammatory mediators: TNF-α, IL-6, IL-8 RANTES. was found be primary contributor beneficial effects. Eubiotic ameliorated mediated disruption leakage, whereas treatment exacerbated These findings indicate role for future prophylactic strategies.
Language: Английский
Citations
14
Probiotics and Antimicrobial Proteins, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 17, 2025
Language: Английский
Citations
0
Microbiology Spectrum, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 25, 2025
ABSTRACT Bacterial vaginosis (BV) is a type of vaginal inflammation caused by bacterial overgrowth, upsetting the healthy microbiome vagina. Existing clinical testing for BV primarily based upon physical and microscopic examination secretions. Modern PCR-based tests target panels BV-associated microbes, such as Labcorp NuSwab test that targets Atopobium ( Fannyhessea ) vaginae , Megasphaera-1 Vaginosis Associated Bacterium (BVAB)−2 . Remnant clinician-collected swabs underwent DNA extraction 16S V3–V4 rRNA gene sequencing to profile microbes in addition those included test. Community state types (CSTs) were determined using most abundant taxon detected each sample. PCR results panel microbial compared against corresponding profiles. Metabolic pathway abundances characterized via metagenomic prediction from amplicon sequence variants (ASVs). 75 remnant yielded 492 unique ASVs, identifying 83 genera. microbe quantification was strongly concordant with P < 0.01). Samples CST-I (18 18, 100%), CST-II (three three, CST-III (15 17, 88%), CST-V (one one, 100%) largely categorized BV-negative panel, while CST-IV samples (28 36, 78%) BV-positive or BV-indeterminate. predicted metabolic signatures shared across multiple CSTs. These findings highlight robust sequencing-based accurate discrimination CSTs dominated distinct Lactobacilli expanded identification biomarkers. IMPORTANCE poses significant health burden women during reproductive years onward. Current diagnostics rely on either select evaluations technicians. Here, we sequenced profiles previously diagnosed better understand disruptions BV. We show can faithfully reproduce targeted diagnostic improve our knowledge This work highlights robust, agnostic classification scheme potential future development tools.
Language: Английский
Citations
0
BMC Microbiology, Journal Year: 2025, Volume and Issue: 25(1)
Published: April 2, 2025
In vitro studies have demonstrated the modulation of vaginal microbiota (VM) by cervical peptides which levels varied with status HPV infection and intraepithelial neoplasia (CIN) grades. However, there is a deficiency in population-based investigating VM compositions metabolome differentially expressed genes (DEGs) across different grades CIN. This study included 43 HPV-positive women, classified into low-grade (CIN1, n = 23) high-grade (CIN2 + , 20) groups. Vaginal swabs were collected for both analysis. Cervical exfoliated cells RNA-Seq We identified 258 (DEGs), among 176 CIN1-enriched linked to immune responses, cell chemotaxis, negative regulation migration, B differentiation, activation, proliferation. Eighty-two upregulated CIN2 cohorts associated epidermis development keratinization. Then, we 5,686 paired correlations between DEGs, VM, metabolome, 2,320 involving Lactobacillus. Further analysis revealed Lactobacillus as primary determinant metabolic profiles, followed Gardnerella, Faecalibacterium, Aerococcus Streptococcus, such notable positive correlation D-lactic acid DL-indole-3-lactic acid. Applying mediation analysis, found that mediated association 14 COL4A2, CCBE1 SPON1, production 57 metabolites, including acid, oleic various amino acids. Additional indicated significant effects 79 metabolites on DEGs growth Lactobacillus, Fannyhessea Aerococcus. Our findings provide valuable evidence inferred correlated CIN stages.
Language: Английский
Citations
0
Diagnostics, Journal Year: 2025, Volume and Issue: 15(9), P. 1090 - 1090
Published: April 25, 2025
Background: Bacterial vaginosis (BV) is considered the most common cause of vaginal discharge, which related to several public health issues, such as an increased risk for sexually transmitted infections, pelvic inflammatory disease, pregnancy-related problems abortion, stillbirth or premature birth, and tubal factor infertility. BV not infection but imbalance in microbiota, characterized by a substitution normal Lactobacilli flora anaerobe. Reducing resistance against infections mechanisms, including bacterial homeostasis, stabilization acid pH, inhibition pathogens adhesion polyamine degradation, production anti-inflammatory molecules, surfactants, antimicrobial substances like hydrogen peroxide, acids, bacteriocins. Approximately half women with can experience symptoms, mainly include malodor, fishy stinging sensation, pH. The treatment based primarily on promoting restoration eliminating dangerous microbiota antibiotic therapy. However, there high rate recurrence relapse. Objective: Based current literature, this review aims propose list ten hallmarks: dysbiosis, inflammation, apoptosis, pH basification, mucosal barrier integrity, pathway activation, epithelial damage, genomic instability, oxidative stress (OS), metabolic reconfiguration. Conclusions: Understanding causes pathogenicity mechanisms critical preventing improving therapeutic management patients.
Language: Английский
Citations
0
BMC Bioinformatics, Journal Year: 2025, Volume and Issue: 26(1)
Published: April 29, 2025
The number and size of multi-omics datasets with paired measurements the host microbiome is rapidly increasing advance sequencing technologies. As it becomes routine to generate these datasets, computational methods aid in their interpretation become increasingly important. Here, we present a framework for integration data: Latent Interacting Variable Effects (LIVE) modeling. LIVE integrates data using single-omic latent variables (LV) organized structured meta-model determine combinations features most predictive phenotype or condition. We developed supervised version leveraging sparse Partial Least Squares Discriminant Analysis (sPLS-DA) LVs, an unsupervised Principal Component (sPCA) principal components which both can incorporate covariate awarness. performance was tested on publicly available metagenomic metabolomics set from Crohn's Disease (CD) Ulcerative Colitis (UC) status patients PRISM LLDeep cohorts, benchmarked against existing gut approaches vaginal datasests, achieving consistent comparable performances. In addition benchmarking efforts, detailed analysis versions cohorts. reduced feature interactions original CD UC millions less than 20,000 while conditioning disease-predictive power microbes, metabolites, enzymes, clinical variables. makes distinct, complementary contribution current integrate offers key advantages interpretable predict disease outcomes identify mechanisms disease.
Language: Английский
Citations
0
Frontiers in Microbiomes, Journal Year: 2024, Volume and Issue: 3
Published: Oct. 11, 2024
A healthy vaginal microbiome (VMB) is dominated by Lactobacillus spp. and provides the first line of defense against invading pathogens. Vaginal dysbiosis, characterized loss dominance increase microbial diversity, has been linked to an increased risk adverse genital tract diseases, including bacterial vaginosis, aerobic vaginitis, vulvovaginal candidiasis, sexually transmitted infections, pregnancy complications such as preterm birth. Currently, antibiotics antifungals are recommended first-line treatments with high cure rates, but they also can lead recurrence resistance development. As alternative, lactobacilli have utilized restore microbiota. In this review article, we discuss dysbiosis in various gynecological infections potential interventions based on Live Biotherapeutic Products (LBPs) a focus those that use intravaginal treatment modalities modulate VMB. Based these, provide insights key factors consider designing phenotypic genotypic screens for selecting strains vaginally administered microbiome-directed therapeutics. Lastly, highlight current progress within field, overview LBPs currently being developed published clinical trial completion recurrent BV, VVC, UTI. We regulatory challenges drug development process harmonize future research efforts VMB therapy.
Language: Английский
Citations
3
Current Opinion in HIV and AIDS, Journal Year: 2024, Volume and Issue: 19(5), P. 234 - 240
Published: June 18, 2024
Purpose of review Among women, having a nonoptimal, highly diverse vaginal microbiome dominated by bacteria other than optimal Lactobacillus species such as L. crispatus or jensenii predicts HIV transmission. Reducing acquisition among women requires better understanding the mechanisms through which impacts transmission dynamics and how to more effectively treat intervene. Technological advancements are improving ability researchers fully characterize interacting host-bacteria mechanisms. Consequently, purpose this was summarize most innovative research on its role in past year. Recent findings Studies combining multiomics, experimental, translational approaches highlight associations nonoptimal with maladaptive alterations immune cell functioning, metabolites, host transcription, mucosal immunity, epithelial barrier integrity. While there multiple proposed increase risk, virtually zero acceptable effective treatments improve immunity. Summary Women-centered solutions modify bacterial metabolites should continue be explored mechanism reduce acquisition.
Language: Английский
Citations
2