Anatomical identification of a corticocortical top-down recipient inhibitory circuitry by enhancer-restricted transsynaptic tracing DOI Creative Commons

Yusuke Atsumi,

Yasuhiro Oisi, Maya Odagawa

et al.

Frontiers in Neural Circuits, Journal Year: 2023, Volume and Issue: 17

Published: Aug. 30, 2023

Despite the importance of postsynaptic inhibitory circuitry targeted by mid/long-range projections (e.g., top-down projections) in cognitive functions, its anatomical properties, such as laminar profile and neuron type, are poorly understood owing to lack efficient tracing methods. To this end, we developed a method that combines conventional adeno-associated virus (AAV)-mediated transsynaptic with distal-less homeobox (Dlx) enhancer-restricted expression system label neurons. We called "Dlx Interneuron-SpECific Tracing" (DISECT). applied DISECT corticocortical circuit from secondary motor cortex (M2) primary somatosensory (S1) wild-type mice. First, injected AAV1-Cre into M2, which enabled Cre recombinase M2-input recipient S1 Second, AAV1-hDlx-flex-green fluorescent protein (GFP) transduce GFP neurons Cre-dependent manner. succeeded exclusively labeling S1. Laminar analysis labeled via indicated were distributed superficial deep layers This distribution was aligned density axons projecting M2. further classified types using immunohistochemistry

Language: Английский

Neocortical somatostatin neuron diversity in cognition and learning DOI Creative Commons
Eunsol Park, Matthew B. Mosso, Alison L. Barth

et al.

Trends in Neurosciences, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

2
Neocortical long-range inhibition promotes cortical synchrony and sleep DOI Creative Commons
Jacob M Ratliff, Geoffrey Terral, Stefano Lutzu

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 23, 2024

Behavioral states such as sleep and wake are highly correlated with specific patterns of rhythmic activity in the cortex. During low arousal slow wave sleep, cortex is synchronized dominated by frequency rhythms coordinated across multiple regions. Although recent evidence suggests that GABAergic inhibitory neurons key players cortical state modulation,

Language: Английский

Citations

4
Quantification of the effect of hemodynamic occlusion in two-photon imaging DOI Open Access
Baba Yogesh, Matthias Heindorf, Rebecca Jordan

et al.

Published: Jan. 6, 2025

The last few years have seen an explosion in the number of tools available to measure neuronal activity using fluorescence imaging (Chen et al., 2013; Feng 2019; Jing Sun 2018; Wan 2021). When performed vivo, these measurements are invariably contaminated by hemodynamic occlusion artifacts. In widefield calcium imaging, this problem is well recognized. For two-photon however, effects only been sparsely characterized. Here we perform a quantification changes observed with GFP expression both and imaging. We find that many instances magnitude signal attributable comparable sensors. Moreover, were spatially heterogeneous, over cortical regions across depth, exhibited complex relationship behavior. Thus, important caveat consider when analyzing interpreting not just but also data.

Language: Английский

Citations

0
Quantification of the effect of hemodynamic occlusion in two-photon imaging DOI Open Access
Baba Yogesh, Matthias Heindorf, Rebecca Jordan

et al.

Published: Jan. 6, 2025

The last few years have seen an explosion in the number of tools available to measure neuronal activity using fluorescence imaging (Chen et al., 2013; Feng 2019; Jing Sun 2018; Wan 2021). When performed vivo, these measurements are invariably contaminated by hemodynamic occlusion artifacts. In widefield calcium imaging, this problem is well recognized. For two-photon however, effects only been sparsely characterized. Here we perform a quantification changes observed with GFP expression both and imaging. We find that many instances magnitude signal attributable comparable sensors. Moreover, were spatially heterogeneous, over cortical regions across depth, exhibited complex relationship behavior. Thus, important caveat consider when analyzing interpreting not just but also data.

Language: Английский

Citations

0
Quantification of the effect of hemodynamic occlusion in two-photon imaging DOI Open Access
Baba Yogesh, Matthias Heindorf, Rebecca Jordan

et al.

Published: March 31, 2025

The last few years have seen an explosion in the number of tools available to measure neuronal activity using fluorescence imaging (Chen et al., 2013; Feng 2019; Jing Sun 2018; Wan 2021). When performed vivo, these measurements are invariably contaminated by hemodynamic occlusion artifacts. In widefield calcium imaging, this problem is well recognized. For two-photon however, effects only been sparsely characterized. Here we perform a quantification changes observed with GFP expression both and imaging. We find that many instances magnitude signal attributable comparable sensors. Moreover, were spatially heterogeneous, over cortical regions across depth, exhibited complex relationship behavior. Thus, important caveat consider when analyzing interpreting not just but also data.

Language: Английский

Citations

0
Type-I nNOS neurons orchestrate cortical neural activity and vasomotion DOI Open Access
Kevin L. Turner,

Dakota F. Brockway,

Md Shakhawat Hossain

et al.

Published: April 9, 2025

It is unknown how the brain orchestrates coordination of global neural and vascular dynamics. We sought to uncover role a sparse but unusual population genetically-distinct interneurons known as type-I nNOS neurons, using novel pharmacological strategic unilaterally ablate these neurons from somatosensory cortex mice. Region-specific ablation produced changes in both activity dynamics, decreased power delta-band local field potential, reduced sustained responses prolonged sensory stimulation, abolished post-stimulus undershoot cerebral blood volume. Coherence between left right gamma-band envelope volume at ultra-low frequencies was decreased, suggesting type-1 integrate long-range signals. Lastly, we observed decreases amplitude resting-state oscillations vasomotion following neurons. This demonstrates that small nNOS-positive are indispensable for regulating dynamics whole implicates disruption diseases ranging neurodegeneration sleep disturbances.

Language: Английский

Citations

0
Type-I nNOS neurons orchestrate cortical neural activity and vasomotion DOI Open Access
Kevin L. Turner,

Dakota F. Brockway,

Md Shakhawat Hossain

et al.

Published: April 9, 2025

It is unknown how the brain orchestrates coordination of global neural and vascular dynamics. We sought to uncover role a sparse but unusual population genetically-distinct interneurons known as type-I nNOS neurons, using novel pharmacological strategic unilaterally ablate these neurons from somatosensory cortex mice. Region-specific ablation produced changes in both activity dynamics, decreased power delta-band local field potential, reduced sustained responses prolonged sensory stimulation, abolished post-stimulus undershoot cerebral blood volume. Coherence between left right gamma-band envelope volume at ultra-low frequencies was decreased, suggesting type-1 integrate long-range signals. Lastly, we observed decreases amplitude resting-state oscillations vasomotion following neurons. This demonstrates that small nNOS-positive are indispensable for regulating dynamics whole implicates disruption diseases ranging neurodegeneration sleep disturbances.

Language: Английский

Citations

0
Parvalbumin interneuron activity induces slow cerebrovascular fluctuations in awake mice DOI Creative Commons
Adiya Rakymzhan, Mitsuhiro Fukuda, Takashi D.Y. Kozai

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 16, 2024

Abstract Neuronal regulation of cerebrovasculature underlies brain imaging techniques reliant on cerebral blood flow (CBF) changes. However, interpreting these signals requires understanding their neural correlates. Parvalbumin (PV) interneurons are crucial in network activity, but impact CBF is not fully understood. Optogenetic studies show that stimulating cortical PV induces diverse responses, including rapid increases, decreases, and slower delayed increases. To clarify this relationship, we measured hemodynamic responses to optogenetic stimulation expressing Channelrhodopsin-2 during evoked ongoing resting-state activity the somatosensory cortex awake mice. Two-photon microscopy (2P) Ca2+ showed robust activation PV-positive (PV+) cells inhibition PV-negative (PV-) cells. Prolonged PV+ cell led a delayed, slow increase, resembling secondary peak response whisker stimulation. 2P vessel diameter measurements revealed induced arterial vasodilation superficial layers deeper layers. Ongoing recordings indicated both PV-cell populations modulate fluctuations at rest, with having greater impact. These findings generate complex depth-dependent vascular response, dominated by changes

Language: Английский

Citations

0
Quantification of the effect of hemodynamic occlusion in two-photon imaging DOI Creative Commons
Baba Yogesh, Matthias Heindorf, Rebecca Jordan

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 29, 2024

ABSTRACT The last few years have seen an explosion in the number of tools available to measure neuronal activity using fluorescence imaging (Chen et al., 2013; Feng 2019; Jing Sun 2018; Wan 2021). When performed vivo, these measurements are invariably contaminated by hemodynamic occlusion artifacts. In widefield calcium imaging, this problem is well recognized. For two-photon however, effects only been sparsely characterized. Here we perform a quantification changes observed with GFP expression both and imaging. We find that many instances magnitude signal attributable comparable sensors. Moreover, were spatially heterogeneous, over cortical regions across depth, exhibited complex relationship behavior. Thus, important caveat consider when analyzing interpreting not just but also data.

Language: Английский

Citations

0
Somatostatin-expressing interneurons induce early NO-driven and late specific astrocyte-mediated vasodilation DOI Creative Commons
Thanh Tan Vo, Won Beom Jung, Tong Huan Jin

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 4, 2024

Abstract Somatostatin-expressing (SST) interneurons modulate hemodynamic responses both directly and indirectly, but their precise role remains unclear. Here, we investigated how SST affect hemodynamics using a combination of electrophysiology, intrinsic optical imaging, calcium fMRI with pharmacological, optogenetic, chemogenetic manipulations. Prolonged optogenetic stimulation neurons induces fast vasodilation through nitric oxide synthase-expressing that co-express SST, slow mediated by astrocytes. Pharmacological blocking receptors suppresses astrocyte activity delayed vasodilation, while early unchanged. Similar neurovascular coupling mechanisms are observed during prolonged sensory stimulation, which also vasodilation. The the SST-astrocyte pathway, enhances specificity cerebral blood volume-weighted signals to cortical layer 4, as confirmed inhibition neurons. Our findings suggest neuron-astrocyte-vascular pathway shapes is critical for achieving high-specificity, laminar-resolution fMRI, increasingly pursued in human cognitive studies.

Language: Английский

Citations

0