Logical regulation of endogenous gene expression using programmable, multi-input processing CRISPR guide RNAs DOI Creative Commons

Hansol Kang,

Dong-Won Park, Jongmin Kim

et al.

Nucleic Acids Research, Journal Year: 2024, Volume and Issue: 52(14), P. 8595 - 8608

Published: June 29, 2024

Abstract The CRISPR-Cas system provides a versatile RNA-guided approach for broad range of applications. Thanks to advances in RNA synthetic biology, the engineering guide RNAs (gRNAs) has enabled conditional control system. However, achieving precise regulation efficient modulation internal metabolic processes remains challenging. In this work, we developed robust dCas9 regulator with engineered gRNAs enable tight endogenous genes. Our Escherichia coli can gene expression upon specific interaction trigger dynamic as high 130-fold, evaluating up three-input logic A OR (B AND C). gRNA-mediated targeting genes, lacZ, malT and poxB, caused differential growth via flux control. Further, could regulate essential cytoskeleton ftsZ mreB, cell filamentation division. Finally, three types two-input gates be applied regulation, resulting morphological changes. successful operation application based on programmable interactions suggests that our compatible other Cas-effectors implemented host organisms.

Language: Английский

Next generation probiotics: Engineering live biotherapeutics DOI
Sanjeeva Kumar Murali, Thomas J. Mansell

Biotechnology Advances, Journal Year: 2024, Volume and Issue: 72, P. 108336 - 108336

Published: March 2, 2024

Language: Английский

Citations

31
The human microbiome and cancer: a diagnostic and therapeutic perspective DOI Creative Commons
Shruthi Kandalai, Huapeng Li, Nan Zhang

et al.

Cancer Biology & Therapy, Journal Year: 2023, Volume and Issue: 24(1)

Published: July 27, 2023

Recent evidence has shown that the human microbiome is associated with various diseases, including cancer. The salivary microbiome, fecal and circulating microbial DNA in blood plasma have all been used experimentally as diagnostic biomarkers for many types of microbiomes present within local tissue, other regions, tumors themselves to promote restrict development progression cancer, most often by affecting cancer cells or host immune system. These microbes also impact efficacy therapies, radiation, chemotherapy, immunotherapy. Here, we review research advances focused on how these different facets why they are important clinical care It only better understanding roles play diagnosis, development, progression, treatment will be able catch treat early.

Language: Английский

Citations

38
Genetic Engineering of Resident Bacteria in the Gut Microbiome DOI Creative Commons
Jack Arnold,

Joshua Glazier,

Mark Mimee

et al.

Journal of Bacteriology, Journal Year: 2023, Volume and Issue: 205(7)

Published: June 29, 2023

Techniques by which to genetically manipulate members of the microbiota enable both evaluation host-microbe interactions and an avenue monitor modulate human physiology. Genetic engineering applications have traditionally focused on model gut residents, such as Escherichia coli lactic acid bacteria. However, emerging efforts develop synthetic biology toolsets for "nonmodel" resident microbes could provide improved foundation microbiome engineering. As genome tools come online, so too novel engineered microbes. Engineered bacteria facilitate investigations roles their metabolites host health allow potential live microbial biotherapeutics. Due rapid pace discovery in this burgeoning field, minireview highlights advancements genetic all

Language: Английский

Citations

24
Macroencapsulated bacteria for in vivo sensing and therapeutics DOI

Yidan Lyu,

Hao Huang, Yuyan Su

et al.

Matter, Journal Year: 2024, Volume and Issue: 7(4), P. 1440 - 1465

Published: Feb. 26, 2024

Language: Английский

Citations

11
Discovery of nanobodies: a comprehensive review of their applications and potential over the past five years DOI Creative Commons

Elena Alexander,

Kam W. Leong

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Oct. 26, 2024

Nanobodies (Nbs) are antibody fragments derived from heavy-chain-only IgG antibodies found in the Camelidae family as well cartilaginous fish. Their unique structural and functional properties, such their small size, ability to be engineered for high antigen-binding affinity, stability under extreme conditions, ease of production, have made them promising tools diagnostics therapeutics. This potential was realized 2018 with approval caplacizumab, world's first Nb-based drug. Currently, Nbs being investigated clinical trials a broad range treatments, including targeted therapies against PDL1 Epidermal Growth Factor Receptor (EGFR), cardiovascular diseases, inflammatory neurodegenerative disorders Alzheimer's disease, Parkinson's amyotrophic lateral sclerosis. They also studied detecting imaging autoimmune conditions infectious diseases severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). A variety methods now available generate target-specific quickly efficiently at low costs, increasing accessibility. article examines these diverse applications roles. Only most recent articles published last five years been used summarize advanced developments field.

Language: Английский

Citations

10
Living Bacteria: A New Vehicle for Vaccine Delivery in Cancer Immunotherapy DOI Open Access
Min Yang, Peiluan Zhong, Pengcheng Wei

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(5), P. 2056 - 2056

Published: Feb. 26, 2025

Cancer vaccines, aimed at evolving the human immune system to eliminate tumor cells, have long been explored as a method of cancer treatment with significant clinical potential. Traditional delivery systems face challenges in directly targeting cells and delivering adequate amounts antigen due hostile microenvironment. Emerging evidence suggests that certain bacteria naturally home on tumors modulate antitumor immunity, making bacterial vectors promising vehicle for precision vaccines. Live vehicles offer several advantages, including colonization, precise drug delivery, stimulation, them compelling option immunotherapy. In this review, we explore mechanisms action behind living bacteria-based recent progress popular chassis, strategies specific payload biocontainment ensure safety. These approaches will lay foundation developing an affordable, widely applicable vaccine system. This review also discusses future opportunities harnessing bacterial-based vaccines enhanced therapeutic outcomes treatment.

Language: Английский

Citations

1
Untranslated region engineering strategies for gene overexpression, fine-tuning, and dynamic regulation DOI
Jun Ren, Sewon Oh, Dokyun Na

et al.

The Journal of Microbiology, Journal Year: 2025, Volume and Issue: 63(3), P. e2501033 - e2501033

Published: March 28, 2025

Language: Английский

Citations

1
Microbiota and IBD: Current knowledge and future perspectives DOI
Caterina Foppa,

Tommy Rizkala,

Alessandro Repici

et al.

Digestive and Liver Disease, Journal Year: 2023, Volume and Issue: 56(6), P. 911 - 922

Published: Nov. 25, 2023

Language: Английский

Citations

22
Microbe-material hybrids for therapeutic applications DOI
Meng Chen, Lili Xia, Chenyao Wu

et al.

Chemical Society Reviews, Journal Year: 2024, Volume and Issue: 53(16), P. 8306 - 8378

Published: Jan. 1, 2024

As natural living substances, microorganisms have emerged as useful resources in medicine for creating microbe-material hybrids ranging from nano to macro dimensions. The engineering of microbe-involved nanomedicine capitalizes on the distinctive physiological attributes microbes, particularly their intrinsic "living" properties such hypoxia tendency and oxygen production capabilities. Exploiting these remarkable characteristics combination with other functional materials or molecules enables synergistic enhancements that hold tremendous promise improved drug delivery, site-specific therapy, enhanced monitoring treatment outcomes, presenting substantial opportunities amplifying efficacy disease treatments. This comprehensive review outlines microbial derivatives used biomedicine specific advantages therapeutic application. In addition, we delineate fundamental strategies mechanisms employed constructing hybrids. diverse biomedical applications constructed hybrids, encompassing bioimaging, anti-tumor, anti-bacteria, anti-inflammation diseases therapy are exhaustively illustrated. We also discuss current challenges prospects associated clinical translation hybrid platforms. Therefore, unique versatility potential exhibited by position them promising candidates development next-generation biomaterials theranostic functionalities.

Language: Английский

Citations

7
Trends and Technology Development in Life Science DOI Open Access

С. Прохоров Е

Published: July 30, 2023

Life Science can divide into many branches. In each branch, scope of research is enormous. The future the technology driven which help to solve problems in daily life. book entitled “Trends and Technology Development Sciences” provides knowledge on some recent trends keep us updated same. It enhances diagnosis; treatment diseases artificial intelligence perform more complex tasks. India one fastest growing markets industry. pandemic made this field think innovative way take up challenges such as vaccine production. Manufacturing pharmaceuticals, biotechnology-based food medicines, medical devices, biomedical technologies, nutraceuticals, environmental science, cloud technology, etc. are important fields development happening all these branches.Collaboration vital between academia industry funding crucial for development. Recent boost good services, simplify processes cost cutting. Moreover, learning surrounding interest detect solution most glitches. Advancement essential country population. Study merits demerits equally sustainable science. book, techniques different included.

Language: Английский

Citations

15