Exercise Training and Cold Exposure Trigger Distinct Molecular Adaptations to Inguinal White Adipose Tissue DOI Creative Commons
Maria Vamvini, Pasquale Nigro, Tiziana Caputo

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 18, 2023

ABSTRACT Exercise training and cold exposure both improve systemic metabolism, but the mechanisms are not well-established. We tested hypothesis that adaptations to inguinal white adipose tissue (iWAT) critical for these beneficial effects by determining impact of exercise-trained cold-exposed iWAT on glucose metabolism proteome secretome. Transplanting trained into sedentary mice improved tolerance, while transplantation showed no such benefit. Compared training, led more pronounced alterations in secretome, downregulating >2,000 proteins also boosting iWAT’s thermogenic capacity. In contrast, only increased extracellular space vesicle transport proteins, upregulated correlate with favorable fasting glucose, suggesting fundamental changes mediate tissue-to-tissue communication. This study defines unique exercise training- exposure-induced proteomes, revealing distinct interventions metabolic health. GRAPHICAL

Language: Английский

Aging and homeostasis of the hypodermis in the age-related deterioration of skin function DOI Creative Commons
Meiqi Liu, Feng Lu, Jingwei Feng

et al.

Cell Death and Disease, Journal Year: 2024, Volume and Issue: 15(6)

Published: June 24, 2024

Abstract Adipose tissues in the hypodermis, crucial stem cell reservoir skin and endocrine organ for maintenance of homeostasis undergo significant changes during aging. Dermal white adipose tissue (dWAT) has recently been recognized as an important both non-metabolic metabolic health regeneration rejuvenation. Defective differentiation, adipogenesis, improper adipocytokine production, immunological dissonance dysfunction dWAT lead to age-associated clinical changes. Here, we review age-related alterations across levels, emphasizing mechanisms underlying regulation We also discuss pathogenic involved fat unfavorable consequences accelerated aging, such chronic inflammaging, immunosenescence, delayed wound healing, fibrosis. Research shown that aging is early initiation event a potential target extending longevity. believe play essential role form therapeutic treatment diseases. Further research needed improve our understanding this phenomenon.

Language: Английский

Citations

17
Energy competition remodels the metabolic glucose landscape of psoriatic epidermal cells DOI Creative Commons
Weiwei Liu, Jingwei Jiang,

Zeming Li

et al.

Theranostics, Journal Year: 2024, Volume and Issue: 14(8), P. 3339 - 3357

Published: Jan. 1, 2024

Rationale: Skin cells actively metabolize nutrients to ensure cell proliferation and differentiation.Psoriasis is an immune-disorder-related skin disease with hyperproliferation in epidermal keratinocytes increasingly recognized be associated metabolic disturbance.However, the adaptations underlying mechanisms of psoriatic remain largely unknown.Here, we explored role competition differentiation skin.Methods: Bulk-and single-cell RNA-sequencing, spatial transcriptomics, glucose uptake experiments were used analyze differences psoriasis.Functional validation vivo vitro was done using imiquimod-like mouse models inflammatory organoid models.Results: We observed highly proliferative basal psoriasis act as winners from suprabasal cells.Using analysis, found that "winner cells" promote OXPHOS pathway upregulation by COX7B lead increased ROS through metabolism, thereby promoting psoriasis.Also, prevent toxic damage ROS, activate glutathione increase their antioxidant capacity assist progression.We further promotes development modulating activity PPAR signaling bulk RNA-seq analysis.We also starvation high expression SLC7A11 causes disulfide stress affects actin cytoskeleton, leading immature skin.Conclusion: Our study demonstrates essential cellular for tissue homeostasis.

Language: Английский

Citations

8
Microfluidic platform for omics analysis on single cells with diverse morphology and size: A review DOI
Shujing Lin, Dan Feng, Xiao Han

et al.

Analytica Chimica Acta, Journal Year: 2024, Volume and Issue: 1294, P. 342217 - 342217

Published: Jan. 6, 2024

Language: Английский

Citations

6
Single-cell insights into HNSCC tumor heterogeneity and programmed cell death pathways DOI

Yuanhao Chai,

Jianlin Zhang, Wenwen Shao

et al.

Translational Oncology, Journal Year: 2025, Volume and Issue: 54, P. 102341 - 102341

Published: March 10, 2025

Language: Английский

Citations

0
Single-nucleus RNA sequencing reveals dynamic changes in the microenvironment of visceral adipose tissue and metabolic characteristics after cold exposure DOI Creative Commons

Ting Yi,

Shuai Wu,

Yang Yu-sha

et al.

Frontiers in Endocrinology, Journal Year: 2025, Volume and Issue: 16

Published: March 24, 2025

Introduction Visceral adipose tissue (VAT) plays a crucial role in regulating systemic metabolic balance. Excess accumulation of VAT is closely associated with various disorders, process that involves the coordinated actions multiple cell types within tissue. Cold exposure, as potential intervention, has been proposed to improve dysfunction. However, heterogeneity and its comprehensive characteristics under cold exposure remain unclear. Methods We collected epididymal white (eWAT) C57BL/6J mice after at three different time points for single-nucleus RNA sequencing (snRNA-seq) analysis. Results successfully identified ten major eWAT, enabling understanding dynamic changes eWAT microenvironment features during exposure. This study revealed 1 day reduced cellular activity intercellular communication including receptor-ligand-based metabolite-mediated interactions. 14 days acclimation, adipocytes was significantly enhanced, restored. Additionally, prolonged promoted formation distinct adipocyte subpopulation may be UCP1-independent thermogenesis. These new homeostatic state established by adapt environment. The also importance adipocytes, stem progenitor cells, myeloid endothelial cells adaptation. Discussion research provides valuable insights into heterogeneity, remodeling, reprogramming It highlights critical transcriptional dynamics perspectives on prevention treatment diseases.

Language: Английский

Citations

0
Single-cell sequencing reveals PHLDA1-positive smooth muscle cells promote local invasion in head and neck squamous cell carcinoma DOI
Bing Guo, Xutao Wen,

Shun Yu

et al.

Translational Oncology, Journal Year: 2025, Volume and Issue: 55, P. 102301 - 102301

Published: March 25, 2025

Language: Английский

Citations

0
A Prognostic Index Derived From LASSO-Selected Preoperative Inflammation and Nutritional Markers for Non–Muscle-Invasive Bladder Cancer DOI
Dengxiong Li,

Ruicheng Wu,

Jie Wang

et al.

Clinical Genitourinary Cancer, Journal Year: 2024, Volume and Issue: 22(3), P. 102061 - 102061

Published: Feb. 29, 2024

Language: Английский

Citations

2
An endothelial-related prognostic index for bladder cancer patients DOI Creative Commons
Dengxiong Li,

Ruicheng Wu,

Jie Wang

et al.

Discover Oncology, Journal Year: 2024, Volume and Issue: 15(1)

Published: April 25, 2024

Abstract Background Within the tumor microenvironment, endothelial cells hold substantial sway over bladder cancer (BC) prognosis. Herein, we aim to elucidate impact of on BC patient outcomes by employing an integration single-cell and bulk RNA sequencing data. Methods All data utilized in this study were procured from online databases. R version 3.6.3 relevant packages harnessed for development validation endothelial-associated prognostic index (EPI). Results EPI was formulated, incorporating six genes (CYTL1, FAM43A, GSN, HSPG2, RBP7, SLC2A3). demonstrated significant value both The Cancer Genome Atlas (TCGA) externally validated dataset. Functional results revealed a profound association between cell functionality, as well immune-related processes. Our findings suggest that patients with low-risk scores are more likely respond positively immunotherapy, indicated immune checkpoint activity, infiltration, mutational burden, stemness index, TIDE, IMvigor210 analyses. Conversely, individuals high-risk exhibited heightened sensitivity cisplatin, docetaxel, gemcitabine treatment regimens. Conclusion We have effectively discerned pivotal perspective constructed patients, thereby offering promising prospects precision medicine.

Language: Английский

Citations

2
Dissecting Spatiotemporal Structures in Spatial Transcriptomics via Diffusion-based Adversarial Learning DOI Creative Commons
Haiyun Wang, Jianping Zhao, Qing Nie

et al.

Research, Journal Year: 2024, Volume and Issue: 7

Published: Jan. 1, 2024

Recent advancements in spatial transcriptomics (ST) technologies offer unprecedented opportunities to unveil the heterogeneity of gene expression and cell states within tissues. Despite these capabilities ST data, accurately dissecting spatiotemporal structures (e.g., domains, temporal trajectories, functional interactions) remains challenging. Here, we introduce a computational framework, PearlST ( p artial differential equation [PDE]- e nhanced dversa r ia l graph autoencoder ), for accurate inference from data using PDE-enhanced adversarial autoencoder. employs contrastive learning extract histological image features, integrates PDE-based diffusion model enhance characterization features at domain boundaries, learns latent low-dimensional embeddings via Wasserstein regularized autoencoders. Comparative analyses across multiple datasets with varying resolutions demonstrate that outperforms existing methods clustering, trajectory inference, pseudotime analysis. Furthermore, elucidates regulations by linking intercellular ligand–receptor interactions most contributing genes embeddings, as illustrated human breast cancer dataset. Overall, proves be powerful tool extracting interpretable intricate various biological contexts.

Language: Английский

Citations

2
Network Meta-Analysis: Effect of Cold Stress on the Gene Expression of Swine Adipocytes ATGL, CIDEA, UCP2, and UCP3 DOI Creative Commons
Zhenhua Guo, Lei Lv, Di Liu

et al.

Current Issues in Molecular Biology, Journal Year: 2024, Volume and Issue: 46(5), P. 3866 - 3876

Published: April 25, 2024

Cold stress significantly affects gene expression in adipocytes; studying this phenomenon can help reveal the pathogeneses of conditions such as obesity and insulin resistance. Adipocyte triglyceride lipase (ATGL); cell death-inducing deoxyribonucleic acid (DNA) fragmentation factor subunit alpha (DFFA)-like effector (CIDEA); uncoupling protein genes UCP1, UCP2, UCP3 are most studied pig adipose tissues under cold stress. However, contradictory results have been observed changes to UCP2 when were examined. Therefore, we conducted a meta-analysis 32 publications total on effect ATGL, CIDEA, UCP3. Our showed that affected swine adipocyte genes; specifically, it was positively correlated with adipocytes. Conversely, ATGL negatively conditions. In addition, loss functional UCP1 pigs likely triggered compensatory increase activity. We also simulated docking could strongly bind adenosine triphosphate (ATP), meaning played more significant role

Language: Английский

Citations

1