Morphological and biomechanical analyses of the human healthy and glaucomatous aqueous outflow pathway: Imaging-to-modeling

Computer Methods and Programs in Biomedicine, Journal Year: 2023, Volume and Issue: 236, P. 107485 - 107485

Published: March 15, 2023

Intraocular pressure (IOP) is maintained via a dynamic balance between the production of aqueous humor and its drainage through trabecular meshwork (TM), juxtacanalicular connective tissue (JCT), Schlemm's canal (SC) endothelium conventional outflow pathway. Primary open angle glaucoma (POAG) often associated with IOP elevation that occurs due to an abnormally high resistance across Outflow tissues are viscoelastic actively interact dynamics two-way fluid-structure interaction coupling. While affects morphology stiffness tissues, their biomechanics hydrodynamics in eyes remain largely unknown. This research aims develop image-to-model method allowing pathway be studied. We used combination X-ray computed tomography scanning electron microscopy reconstruct high-fidelity, eye-specific, 3D microstructural finite element models healthy cellularized decellularized conditions. The TM/JCT/SC complex embedded beam elements were subjected physiological load boundary; stresses/strains flow state calculated using computational fluid dynamics. Based on resultant parameters pathway, primary site was JCT immediate vicinity SC inner wall, while majority occurred TM. TM showed 1.32-fold 1.13-fold larger thickness smaller space size (2.24-fold 1.50-fold) compared eyes. Characterizing accurate may significantly contribute constructing more accurate, robust, reliable models, can eventually help better understand regulation, humor, human model demonstrates proof concept for determining changes glaucomatous thus utilized cohorts donor where disease specificity, race, age, gender eye donors accounted for.

Language: Английский

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The Effect of Intraocular Pressure Load Boundary on the Biomechanics of the Human Conventional Aqueous Outflow Pathway

Bioengineering, Journal Year: 2022, Volume and Issue: 9(11), P. 672 - 672

Published: Nov. 10, 2022

Aqueous humor outflow resistance in the trabecular meshwork (TM), juxtacanalicular connective tissue (JCT), and Schlemm's canal (SC) endothelium of conventional pathway actively contribute to intraocular pressure (IOP) regulation. Outflow is affected by dynamic gradient across TM, JCT, SC inner wall tissues. The effect implies presence a fluid-structure interaction (FSI) coupling between tissues aqueous humor. However, biomechanical interactions viscoelastic dynamics are largely unknown. A 3D microstructural finite element (FE) model healthy human eye TM/JCT/SC complex was constructed with elastic material properties for bulk extracellular matrix embedded cable elements. FE models were subjected both idealized physiologic IOP load boundary using FSI method. at equal IOPs showed similar stresses strains as well sensitive rate, resulting different mechanical hydrodynamic responses Transient fluctuations may cause relatively large difference ~20 mmHg very short time frame ~0.1 s, rate stiffening Rate reduces causes rate-dependent Thus, results suggest it necessary use that includes important role rate.

Language: Английский

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Developing an experimental-computational workflow to study the biomechanics of the human conventional aqueous outflow pathway

Acta Biomaterialia, Journal Year: 2023, Volume and Issue: 164, P. 346 - 362

Published: April 16, 2023

Language: Английский

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Impact of aging on anterior segment morphology and aqueous humor dynamics in human Eyes: Advanced imaging and computational techniques

Journal of Applied Biomedicine, Journal Year: 2025, Volume and Issue: 45(1), P. 62 - 73

Published: Jan. 1, 2025

Language: Английский

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Matrix stiffness regulates traction forces, cytoskeletal dynamics, and collagen reorganization in trabecular meshwork cells in glaucoma

Matter, Journal Year: 2025, Volume and Issue: unknown, P. 102094 - 102094

Published: April 1, 2025

Language: Английский

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Segmental biomechanics of the normal and glaucomatous human aqueous outflow pathway

Acta Biomaterialia, Journal Year: 2023, Volume and Issue: 173, P. 148 - 166

Published: Nov. 7, 2023

Language: Английский

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Comparative analysis of traction forces in normal and glaucomatous trabecular meshwork cells within a 3D, active fluid-structure interaction culture environment

Acta Biomaterialia, Journal Year: 2024, Volume and Issue: 180, P. 206 - 229

Published: April 17, 2024

Language: Английский

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Modeling the Endothelial Glycocalyx Layer in the Human Conventional Aqueous Outflow Pathway

Cells, Journal Year: 2022, Volume and Issue: 11(23), P. 3925 - 3925

Published: Dec. 4, 2022

A layer of proteoglycans and glycoproteins known as glycocalyx covers the surface trabecular meshwork (TM), juxtacanalicular tissue (JCT), Schlemm’s canal (SC) inner wall conventional aqueous outflow pathway in eye. This has been shown to play a role mechanotransduction fluid shear stress regulation resistance. The resistance is main determinant intraocular pressure (IOP) through an active, two-way, fluid–structure interaction coupling between tissues humor. 3D microstructural finite element (FE) model healthy human eye TM/JCT/SC complex with interspersed humor was constructed. very thin charged double that represents endothelial covered elastic tissues. modeled electroosmotic flow when it contact electrical–fluid–structure (EFSI) method used couple (glycocalyx), (aqueous humor), solid (outflow tissues). When IOP elevated 15 mmHg, maximum velocity EFSI decreased by 2.35 mm/s (9%) compared (FSI) model. charge or electricity living generated plays minor biomechanical resultant stresses strains well hydrodynamics

Language: Английский

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Biomechanics of the JCT and SC Inner Wall Endothelial Cells with Their Basement Membrane Using 3D Serial Block-Face Scanning Electron Microscopy

Bioengineering, Journal Year: 2023, Volume and Issue: 10(9), P. 1038 - 1038

Published: Sept. 4, 2023

Background: More than ~70% of the aqueous humor exits eye through conventional outflow pathway that is comprised trabecular meshwork (TM), juxtacanalicular tissue (JCT), inner wall endothelium Schlemm’s canal (SC). The flow resistance in JCT and SC basement membrane thought to play an important role regulation intraocular pressure (IOP) eye, but current imaging techniques do not provide enough information about mechanics these tissues or this area. Methods: A normal human was perfusion-fixed a radial wedge TM from high-flow region dissected. were then sliced imaged using serial block-face scanning electron microscopy. Slices images selected segmented create 3D finite element model cells with membrane. used replace intertrabecular spaces, pores, giant vacuoles, fluid–structure interaction employed couple motion humor. Results: Higher tensile stresses (0.8-kPa) strains (25%) observed beneath vacuoles open pores. volumetric average shear stress higher JCT/SC. As approached SC, velocity decreased, resulting formation small eddies immediately after left wall. Conclusions: Improved modeling can enhance our understanding funneling. Serial microscopy achieve this, micro-segmental patterns ex vivo perfused eyes suggest hypothetical mechanism.

Language: Английский

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Implementing new computational methods for the study of JCT and SC inner wall basement membrane biomechanics and hydrodynamics

Computer Methods and Programs in Biomedicine, Journal Year: 2023, Volume and Issue: 243, P. 107909 - 107909

Published: Oct. 31, 2023

Language: Английский

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