Interface engineering of cellobiose dehydrogenase improves interdomain electron transfer DOI Creative Commons
Thomas M. B. Reichhart, Stefan Scheiblbrandner, Christoph Sygmund

et al.

Protein Science, Journal Year: 2023, Volume and Issue: 32(8)

Published: June 14, 2023

Abstract Cellobiose dehydrogenase (CDH) is a bioelectrocatalyst that enables direct electron transfer (DET) in biosensors and biofuel cells. The application of this bidomain hemoflavoenzyme for physiological glucose measurements limited by its acidic pH optimum slow interdomain (IET) at 7.5. reason rate‐limiting step electrostatic repulsion the interface between catalytic domain mediating cytochrome (CYT). We applied rational engineering to accelerate IET prevailing blood or interstitial fluid. Phylogenetic structural analyses guided design 17 variants which amino acids were mutated CYT domain. Five mutations (G71K, D160K, Q174K, D177K, M180K) increased rate. Structure‐based analysis suggested two mechanisms explaining improvements: steering stabilization closed state hydrogen bonding. Combining into six combinatorial with up five shifted from 4.5 7.0 7.5 over 12‐fold 0.1 1.24 s −1 . While mutants sustained high enzymatic activity even surpassed wild‐type enzyme, accumulated positive charges on decreased DET, highlighting importance DET. This study shows an effective strategy shift improve CDH, but future work needs maintain DET bioelectronic applications.

Language: Английский

Enzyme-based amperometric biosensors: 60 years later … Quo Vadis? DOI
Paolo Bollella

Analytica Chimica Acta, Journal Year: 2022, Volume and Issue: 1234, P. 340517 - 340517

Published: Oct. 13, 2022

Language: Английский

Citations

43
Enzymatic cofactor regeneration systems: A new perspective on efficiency assessment DOI

Karolina Bachosz,

Jakub Zdarta, Muhammad Bilal

et al.

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 868, P. 161630 - 161630

Published: Jan. 16, 2023

Language: Английский

Citations

40
Recent developments in electrochemical sensors based on graphene for bioanalytical applications DOI Creative Commons
Abdulazeez T. Lawal

Sensing and Bio-Sensing Research, Journal Year: 2023, Volume and Issue: 41, P. 100571 - 100571

Published: July 8, 2023

An effective and affordable technique for detecting a variety of substances biological, medical, environmental significance is electrochemical sensing (ECS). Due to their distinctive structures abilities offer robust electrocatalytic activity with little surface fouling, low cost, biocompatibility, simple electron transfer kinetics, carbon materials-based electrodes like graphene (GR) nanotube (CNT) are frequently used the development sensors. Since GR has large specific area, it can host number biomolecules still have decent detection sensitivity. Along important developments in synthesis, purification, conjugation, biofunctionalization GR, device integration technologies nanofabrication evolved. Combinations aforementioned characteristics accelerated GR-based sensors many critical bio-analyses. The rapid bioanalyses better sensitivity selectivity been facilitated by combination features. Direct transport between enzyme active electrode region made possible application GR. It easily construct execute on-site oxidases dehydrogenases improved thanks excellent electro-catalytic activities on redox reaction hydrogen peroxide (H2O2) nicotinamide adenine dinucleotides (NADH). GR's oxidation NADH, thiols, H2O2, other interfering species occurs at potentials, use effectively suppresses these species. two benefits clinical chemistry, food quality control, wastewater treatment, bioprocessing great resistance fouling selectivity.

Language: Английский

Citations

36
Continuous Measurement of Lactate Concentration in Human Subjects through Direct Electron Transfer from Enzymes to Microneedle Electrodes DOI Creative Commons
David M E Freeman, Damien Ming, Richard Wilson

et al.

ACS Sensors, Journal Year: 2023, Volume and Issue: 8(4), P. 1639 - 1647

Published: March 27, 2023

Microneedle lactate sensors may be used to continuously measure concentration in the interstitial fluid a minimally invasive and pain-free manner. First- second-generation enzymatic produce redox-active product that is electrochemically sensed at electrode surface. Direct electron transfer enzymes electrons directly as of action; this study, direct enzyme specific has been immobilized onto microneedle surface create lactate-sensing devices function low applied voltages (0.2 V). These have validated small study human volunteers; concentrations were raised lowered through physical exercise subsequent rest. Lactazyme show good agreement with concurrently obtained analyzed serum levels.

Language: Английский

Citations

32
Protein engineering for electrochemical biosensors DOI
Zhiguang Zhu, Haiyan Song, Yuan-Ming Wang

et al.

Current Opinion in Biotechnology, Journal Year: 2022, Volume and Issue: 76, P. 102751 - 102751

Published: June 28, 2022

Language: Английский

Citations

24
Electrocatalytic NAD(P)H regeneration for biosynthesis DOI Creative Commons

Yaoxuan Li,

Guanhua Liu, Weixi Kong

et al.

Green Chemical Engineering, Journal Year: 2023, Volume and Issue: 5(1), P. 1 - 15

Published: Feb. 1, 2023

The highly efficient chemoselectivity, stereoselectivity, and regioselectivity render enzyme catalysis an ideal pathway for the synthesis of various chemicals in broad applications. While cofactor is necessary but expensive, conversed state not beneficial positive direction reaction. Cofactor regeneration using electrochemical methods has advantages simple operation, low cost, easy process monitoring, product separation, electrical energy green sustainable. Therefore, bioelectrocatalysis great potential by combining with enzymatic catalysis. In this review, we detail mechanism categorize common electron mediators enzymes used regeneration. reaction type recent progress are summarized electrochemically coupled main challenges such electroenzymatic pointed out future developments field foreseen.

Language: Английский

Citations

15
Recent Advances in Applications of Oxidases and Peroxidases Polymer-Based Enzyme Biocatalysts in Sensing and Wastewater Treatment: A Review DOI Open Access
Hilda Dinah Kyomuhimbo, Usisipho Feleni,

Nils Haneklaus

et al.

Polymers, Journal Year: 2023, Volume and Issue: 15(16), P. 3492 - 3492

Published: Aug. 21, 2023

Oxidase and peroxidase enzymes have attracted attention in various biotechnological industries due to their ease of synthesis, wide range applications, operation under mild conditions. Their applicability, however, is limited by poor stability harsher conditions non-reusability. As a result, several approaches such as enzyme engineering, medium immobilization been used improve the properties. Several materials supports for these increase reusability. This review focusses on oxidase metal oxide nanoparticle-polymer composite different methods achieve immobilization. The application enzyme-metal/metal oxide-polymer biocatalysts biosensing hydrogen peroxide, glucose, pesticides, herbicides well blood components cholesterol, urea, dopamine, xanthine extensively reviewed. wastewater treatment through degradation dyes, other organic compounds has also discussed.

Language: Английский

Citations

14
Essential Insight of Direct Electron Transfer-Type Bioelectrocatalysis by Membrane-Bound d-Fructose Dehydrogenase with Structural Bioelectrochemistry DOI Creative Commons
Yohei Suzuki, Fumiaki Makino, Tomoko Miyata

et al.

ACS Catalysis, Journal Year: 2023, Volume and Issue: 13(20), P. 13828 - 13837

Published: Oct. 12, 2023

Flavin adenine dinucleotide-dependent d-fructose dehydrogenase (FDH) from Gluconobacter japonicus NBRC3260, a membrane-bound heterotrimeric flavohemoprotein capable of direct electron transfer (DET)-type bioelectrocatalysis, was investigated the perspective structural biology, bioelectrochemistry, and protein engineering. DET-type reactions offer several benefits in biomimetics (e.g., biofuel cells, bioreactors, biosensors) owing to their mediator-less configuration. FDH provides an intense catalytic signal; therefore, extensive research has been conducted on fundamental principles applications biosensors. Structural analysis using cryo-electron microscopy single-particle revealed entire structures with resolutions 2.5 2.7 Å for reduced oxidized forms, respectively. The (ET) pathway during oxidation by both thermodynamic kinetic approaches. shown localization electrostatic surface charges around heme 2c subunit II, experiments functionalized electrodes controlled charge support notion that is electrode-active site. Furthermore, two aromatic amino acid residues (Trp427 Phe489) were located possible long-range ET between electrode. Two variants (W427A F489A) obtained site-directed mutagenesis, effects activity elucidated. results have Trp427 plays essential role accelerating triples standard rate constant heterogeneous according bioelectrochemical analysis.

Language: Английский

Citations

13
A flexible carbon nanotubes-based microelectrode for non-enzymatic electrochemical uric acid and ascorbic acid sensing DOI
Arif Nazir,

Sheza Muqaddas,

Abid Ali

et al.

Materials Science and Engineering B, Journal Year: 2024, Volume and Issue: 307, P. 117480 - 117480

Published: June 11, 2024

Language: Английский

Citations

5
Structure and function relationship of formate dehydrogenases: an overview of recent progress DOI Creative Commons

Ami Kobayashi,

M. Taketa, Keisei Sowa

et al.

IUCrJ, Journal Year: 2023, Volume and Issue: 10(5), P. 544 - 554

Published: Sept. 1, 2023

Formate dehydrogenases (FDHs) catalyze the two-electron oxidation of formate to carbon dioxide. FDHs can be divided into several groups depending on their subunit composition and active-site metal ions. Metal-dependent (Mo- or W-containing) from prokaryotic organisms belong superfamily molybdenum enzymes are members dimethylsulfoxide reductase family. In this short review, recent progress in structural analysis together with potential biotechnological applications summarized.

Language: Английский

Citations

11