Current Opinion in Electrochemistry, Journal Year: 2023, Volume and Issue: 42, P. 101390 - 101390
Published: Sept. 9, 2023
Language: Английский
Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(11)
Published: Jan. 21, 2024
Abstract Multiplexing is a valuable strategy to boost throughput and improve clinical accuracy. Exploiting the vertical, meshed design of reproducible low‐cost ultra‐dense electrochemical chips, unprecedented single‐response multiplexing typical label‐free biosensors reported. Using cheap, handheld one‐channel workstation single redox probe, that is, ferro/ferricyanide, recognition events taking place on two spatially resolved locations same working electrode can be tracked along voltammetry scan by collecting signatures probe in relation different quasi‐reference electrodes, Au (0 V) Ag/AgCl ink (+0.2 V). This spatial isolation prevents crosstalk between tags interferences over functionalization binding steps, representing an advantage existing non‐spatially multiplex strategies. As proof concept, peptide‐tethered immunosensors are demonstrated provide duplex detection COVID‐19 antibodies, thereby doubling while achieving 100% accuracy serum samples. The approach envisioned enable broad applications high‐throughput multi‐analyte platforms, as it tailored other biosensing devices formats.
Language: Английский
Citations
9
Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(11), P. 4580 - 4588
Published: Feb. 13, 2024
This work reports the first electrochemical bioplatforms developed for determination of total contents either target miRNA or methylated miRNA. The are based on hybridization with a synthetic biotinylated DNA probe, capture formed DNA/miRNA heterohybrids surface magnetic microcarriers, and their recognition an antibody selective to these N6-methyladenosine (m6A) epimark. was accomplished by labeling such secondary antibodies horseradish peroxidase (HRP) enzyme. In both cases, amperometric transduction performed disposable electrodes after capturing resulting HRP-tagged bioconjugates. Because increasing relevance in colorectal cancer (CRC) diagnosis prognosis, let-7a m6A methylation were selected. proposed showed attractive analytical operational characteristics m6A-methylated less than 75 min. These bioplatforms, innovative design application, applied analysis RNA samples extracted from cultured cells different metastatic profiles paired healthy tumor tissues patients diagnosed CRC at stages. obtained results demonstrated, time using platforms, potential interrogating level discriminate capacities identify and, pioneering way, serve as prognostic biomarker CRC.
Language: Английский
Citations
8
Frontiers in Medical Technology, Journal Year: 2024, Volume and Issue: 6
Published: Feb. 15, 2024
Herein, advancements in electroanalytical devices for the simultaneous detection of diverse breast cancer (BC) markers are demonstrated. This article identifies several important areas exploration electrochemical diagnostics and highlights factors that pivotal successful deployment novel bioanalytical devices. We have highlighted limits (LOD) reported multiplex biosensor can surpass sensitivity displayed by current clinical standards such as ELISA, FISH, PCR. HER-2; a marker characterised increased metastatic potential, more aggressive development, poor outcomes; be sensed with LOD 0.5 ng/ml using platforms, which falls within range measured ELISA (from picogram/ml to nanogram/ml). Electrochemical biosensors 0.53 0.21 U/ml MUC-1 CA 15-3, respectively, or 5.8 × 10
Language: Английский
Citations
6
Talanta, Journal Year: 2023, Volume and Issue: 269, P. 125394 - 125394
Published: Nov. 6, 2023
Language: Английский
Citations
14
Sensors and Actuators B Chemical, Journal Year: 2023, Volume and Issue: 404, P. 135196 - 135196
Published: Dec. 21, 2023
Language: Английский
Citations
13
Talanta, Journal Year: 2024, Volume and Issue: 281, P. 126870 - 126870
Published: Sept. 17, 2024
Language: Английский
Citations
4
Current Opinion in Electrochemistry, Journal Year: 2023, Volume and Issue: 42, P. 101411 - 101411
Published: Oct. 13, 2023
Language: Английский
Citations
10
ACS Applied Nano Materials, Journal Year: 2024, Volume and Issue: 7(16), P. 19175 - 19183
Published: Aug. 1, 2024
Chiral channels or pores are a fundamental feature of chiral metal–organic frameworks (CMOFs). However, the roles and have always been overlooked in enantioselective sensing based on CMOFs. Aiming at platforms enantioselectivity with efficient electrochemical recognition, L-His, L-Glu, L-Lys introduced into framework (MOF) as Taking advantage postsynthetic modifications (PSM), resulting CMOFs (MOF-His/MOF-Glu/MOF-Lys) exhibit channel dependence for different amino acid enantiomers. Ascribed to introduction linkers, nanomaterials sizes recognition behaviors glutamate (Glu) enantiomers, tryptophan (Trp) phenylalanine (Phe) which evaluated detail by differential pulse voltammetry (DPV). In addition, ascribed regulation chirality information transmission through introducing molecules sizes, resolved reveal unique adjustable upon addition molecules, leading passage enantiomers excellent efficiency. This work provides not only design strategy developing tunability but also an idea construction platform.
Language: Английский
Citations
3
JACS Au, Journal Year: 2024, Volume and Issue: 4(12), P. 4655 - 4672
Published: Dec. 11, 2024
Electrochemical biosensors are gaining attention as powerful tools in cancer diagnosis, particularly liquid biopsy, due to their high efficiency, rapid response, exceptional sensitivity, and specificity. However, the complexity of intra- intertumor heterogeneity, with variations genetic protein expression profiles epigenetic modifications, makes electrochemical susceptible false-positive or false-negative diagnostic outcomes. To address this challenge, there is growing interest simultaneously analyzing multiple biomarkers reveal molecular characteristics tumor heterogeneity for precise diagnosis. In Perspective, we highlight recent advancements utilizing a specific emphasis on multitarget analysis including tumor-associated nucleic acids, markers, extracellular vesicles, cells. These hold significant promise improving precision early diagnosis monitoring, well potentially offering new insights into personalized management.
Language: Английский
Citations
3
Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown
Published: April 4, 2025
Individually addressable microelectrode arrays (MEAs) enable the simultaneous and independent measurement of multiple analytes benefit from a small size scale, which enables highly localized electrochemical detection. In this work, we describe new methodology to fabricate low-cost tunable MEA probes in number, spatial arrangement, spacing electrodes electrode material can be changed controlled. This was achieved using 3D printed support assembly position wires into designated positions mold seal place epoxy resin. After curing epoxy, mechanical polishing exposed surface closely spaced disk microelectrodes embedded insulating material, formed MEA. The individual array were characterized methods optical electron microscopy evaluate quality integrity with epoxy. To validate fabrication method demonstrate controlled spacing, used dual-disk device, while four-, five-, seven-electrode some different numbers geometric arrangements that are possible. While developed have numerous potential applications, including as or substrates scanning microscopy, fabricated aptamer-based sensors on electrodes, for detection adenosine triphosphate dopamine phosphate-buffered saline solution, without 10% fetal bovine serum.
Language: Английский
Citations
0