Region‐specific brain decellularized scaffolds can recover cell viability in an oxygen-glucose deprivation model DOI Creative Commons
Diego Reginensi, Didio Ortiz, Simón P. Castillo

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 21, 2024

Abstract Brain decellularized extracellular matrix (ECM) can be an attractive scaffold capable of mimicking the native ecosystem central nervous system tissue. In this study, we studied in vitro response neural lineage cells exposed to region-specific brain ECM scaffolds from three distinct neuroanatomical sections: cortex, cerebellum and remaining areas. First, evaluation each subregion was performed with isotropic fractionator method understand cellular composition different cerebral Second, subregions subjected decellularization process their respective characterization using molecular, histological, ultrastructural techniques. Third, presence neurotrophic factors in analyzed. Finally, as a mimetic platform for maturation PC12 recovery cell viability oxygen-glucose deprivation model. Our results show that serve biomimetic promoting growth and, addition, it possesses combination structural biochemical signals (e.g., factors) are inducing phenotypic changes promote stroke/ischemia model vitro.

Language: Английский

Bioinspired semi-flexible hydrogel with anti-inflammatory potential for natural tissue-mimicking bone regeneration DOI
Jae Seo Lee, Hyosung Kim, Haram Nah

et al.

Composites Part B Engineering, Journal Year: 2024, Volume and Issue: 273, P. 111223 - 111223

Published: Jan. 18, 2024

Language: Английский

Citations

13
Dynamic Hydrogel‐Based Strategy for Traumatic Brain Injury Modeling and Therapy DOI Creative Commons
Xin He,

Meng Lei,

Xuewen Chen

et al.

CNS Neuroscience & Therapeutics, Journal Year: 2025, Volume and Issue: 31(1)

Published: Jan. 1, 2025

ABSTRACT Traumatic brain injury (TBI) is one of the most traumatizing and poses serious health risks to people's bodies due its unique pathophysiological characteristics. The investigations on pathological mechanism valid interventions TBI have attracted widespread attention worldwide. With bio‐mimic mechanic cues, dynamic hydrogels with stiffness changes or reversible crosslinking been suggested construct in vitro disease models novel therapeutic agents for TBI. However, there a lack clarification currently reported their biomedical applications Our review starts introducing native mechanical characters then summarizes common chemical strategies dynamically tunable networks modeling therapy. Finally, we prospect future development TBI, providing new insights guidance tailored brain‐targeted biomaterials.

Language: Английский

Citations

1
An injectable extracellular matrix-mimicking conductive hydrogel for sequential treatment of ischemic stroke DOI
Yue Wang, Zhicun Wang, Wenjia Guo

et al.

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: unknown, P. 158039 - 158039

Published: Nov. 1, 2024

Language: Английский

Citations

5
A taurine-based hydrogel with the neuroprotective effect and the ability to promote neural stem cell proliferation DOI
Zhichao Wang,

Chuanzhen Huang,

Zhenyu Shi

et al.

Biomaterials Advances, Journal Year: 2024, Volume and Issue: 161, P. 213895 - 213895

Published: May 21, 2024

Language: Английский

Citations

4
Functionalized hydrogels as smart gene delivery systems to treat musculoskeletal disorders DOI Creative Commons

Mohammadsaeid Enayati,

Wei Liu, Henning Madry

et al.

Advances in Colloid and Interface Science, Journal Year: 2024, Volume and Issue: 331, P. 103232 - 103232

Published: June 14, 2024

Despite critical advances in regenerative medicine, the generation of definitive, reliable treatments for musculoskeletal diseases remains challenging. Gene therapy based on delivery therapeutic genetic sequences has strong value to offer effective, durable options decisively manage such disorders. Furthermore, scaffold-mediated gene provides powerful alternatives overcome hurdles associated with classical therapy, allowing spatiotemporal candidate genes sites injury. Among many scaffolds research, hydrogels raised increasing attention addition other potent systems (solid, hybrid scaffolds) due their versatility and competence as drug cell carriers tissue engineering wound dressing. Attractive functionalities include injectability, stimuli-responsiveness, self-healing, nanocomposition that may further allow upgrade them "intelligently" efficient mechanically platforms, rather than just inert vehicles. Such functionalized also be tuned successfully transfer a minimally invasive manner order protect cargos long-term effects. In light features, this review focuses demonstrates treatment disorders using procedures, from principles hydrogel functionalization methods applications hydrogel-mediated disorders, while remaining challenges are being discussed perspective translation patients. offering Scaffold-mediated therapy. attention. Functionalities including tune This introduces disorder

Language: Английский

Citations

4
ROS-Triggered Biomimetic Hydrogel Soft Scaffold for Ischemic Stroke Repair DOI
Wen Zhang, Yang Liu,

Yu Wu

et al.

Biomaterials, Journal Year: 2025, Volume and Issue: 319, P. 123217 - 123217

Published: Feb. 25, 2025

Language: Английский

Citations

0
Region-specific brain decellularized extracellular matrix promotes cell recovery in an in vitro model of stroke DOI Creative Commons
Diego Reginensi, Didio Ortiz, Bernardino Denis

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 7, 2025

Brain decellularized extracellular matrix (ECM) can be an attractive scaffold capable of mimicking the native ecosystem central nervous system tissue. We studied in vitro response neural cultures exposed to region-specific brain ECM scaffolds from three distinct neuroanatomical sections: cortex, cerebellum and remaining areas. First, each region was evaluated with isotropic fractionator method understand cellular composition different cerebral Second, regions were subjected decellularization process their respective characterization using molecular, histological, ultrastructural techniques. Third, levels neurotrophic factors analyzed. Fourth, we as a mimetic platform for maturation PC12 cells, unidirectional model differentiation. Finally, studies carried out evaluate cell recovery capacity under stroke-mimetic conditions. Our results show that serve biomimetic promoting growth lineage cells and, addition, it possesses combination structural biochemical signals (e.g., factors) are inducing phenotypic changes promote viability stroke/ischemia vitro.

Language: Английский

Citations

0
Design and development of alteplase and borneol inclusion complex incorporated supramolecular hydrogel delivery enhanced the treatment and care of ischemic stroke DOI
Hailing Zhang, Xin Jia, Meiling Yu

et al.

Particulate Science And Technology, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 11

Published: May 2, 2025

Language: Английский

Citations

0
Dual role of electrical stimulation and biomimetic matrix on neural differentiation within the microfluidic platform DOI
Utku Devamoglu, Yagmur Arslan, Leila Sabour Takanlou

et al.

Biomaterials Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Herein, we investigated the enhancing role of various physico-chemical and physico-mechanical factors affecting neural differentiation within microfluidic platform.

Language: Английский

Citations

0
Composite materials combined with stem cells promote kidney repair and regeneration DOI Creative Commons
Hao Tian,

Liulin Wu,

Haoxiang Qin

et al.

Composites Part B Engineering, Journal Year: 2024, Volume and Issue: 275, P. 111278 - 111278

Published: Feb. 5, 2024

Kidney disease is a worldwide public health problem, seriously threatening the quality of life human beings, mainly including acute kidney injury (AKI), chronic (CKD), and end-stage renal (ESRD), with high mortality rate. Current effective treatments include dialysis transplants, but shortages donor organs, immune rejection, other problems have not been effectively addressed. In recent years, stem cells extensively studied, due to proliferation multi-directional differentiation properties, shown great potential for applications in field tissue regeneration, which may be an means repair regeneration. At same time, rapid development composite materials show excellent performance hemostasis, anti-inflammatory, organoids scaffolds construction, expected further guide promoting this review, we discuss latest research progress regeneration from perspectives cell extracellular vesicle (EVs) regulation, material combined used engineering kidneys culture. Besides, analyze summarize mechanisms as well pathways. addition, raise key issues that currently constrain composites therapy disease. Finally, possible future direction

Language: Английский

Citations

2