Metamorphosis of memory circuits in Drosophila reveals a strategy for evolving a larval brain DOI Creative Commons
James W. Truman,

Jacquelyn Price,

Rosa Linda Miyares

et al.

eLife, Journal Year: 2023, Volume and Issue: 12

Published: Jan. 25, 2023

Mushroom bodies (MB) of adult Drosophila have a core thousands Kenyon neurons; axons the early-born g class form medial lobe and those from later-born α'β' αβ classes both vertical lobes. The larva, however, hatches with only γ neurons forms 'facsimile' using larval-specific axon branches its neurons. MB input (MBINs) output (MBONs) divide neuron lobes into discrete computational compartments. larva has 10 such compartments while 16. We determined fates 28 32 MBONs MBINs that define larval Seven are subsequently incorporated MB; four their die, 12 MBINs/MBONs remodel to function in remaining three specific. At metamorphosis MBIN/MBONs trans-differentiate, leaving for other brain circuits. made de novo MBONs/MBINs recruited pools adult-specific combination cell death, compartment shifting, trans-differentiation, recruitment new result no MBIN-MBON connections being maintained through metamorphosis. this simple level, then, we find anatomical substrate memory trace persisting adult. phenotype trans-differentiating represents evolutionarily ancestral is derived adaptation stage. These cells arise primarily within lineages also produce permanent MBONs, suggesting specifying factors may allow information related birth-order or sibling identity be interpreted modified manner these acquire phenotypic modifications. loss at then allows revert functions

Language: Английский

Neuronal wiring diagram of an adult brain DOI Creative Commons
Sven Dorkenwald, Arie Matsliah, Amy Sterling

et al.

Nature, Journal Year: 2024, Volume and Issue: 634(8032), P. 124 - 138

Published: Oct. 2, 2024

Language: Английский

Citations

139
Neuronal wiring diagram of an adult brain DOI Creative Commons
Sven Dorkenwald, Arie Matsliah, Amy Sterling

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: June 30, 2023

Abstract Connections between neurons can be mapped by acquiring and analyzing electron microscopic (EM) brain images. In recent years, this approach has been applied to chunks of brains reconstruct local connectivity maps that are highly informative, yet inadequate for understanding function more globally. Here, we present the first neuronal wiring diagram a whole adult brain, containing 5×10 7 chemical synapses ∼130,000 reconstructed from female Drosophila melanogaster . The resource also incorporates annotations cell classes types, nerves, hemilineages, predictions neurotransmitter identities. Data products available download, programmatic access, interactive browsing made interoperable with other fly data resources. We show how derive projectome, map projections regions, connectome. demonstrate tracing synaptic pathways analysis information flow inputs (sensory ascending neurons) outputs (motor, endocrine, descending neurons), across both hemispheres, central optic lobes. Tracing subset photoreceptors all way motor illustrates structure uncover putative circuit mechanisms underlying sensorimotor behaviors. technologies open ecosystem FlyWire Consortium set stage future large-scale connectome projects in species.

Language: Английский

Citations

134
A neural circuit for wind-guided olfactory navigation DOI Creative Commons
Andrew M. M. Matheson, Aaron J. Lanz,

Ashley M. Medina

et al.

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: Aug. 8, 2022

Abstract To navigate towards a food source, animals frequently combine odor cues about source identity with wind direction location. Where and how these two are integrated to support navigation is unclear. Here we describe pathway the Drosophila fan-shaped body that encodes attractive promotes upwind navigation. We show neurons throughout this encode odor, but not direction. Using connectomics, identify local called h∆C receive input from previously described pathway. exhibit odor-gated, direction-tuned activity, sparse activation of in reproducible direction, activity required for persistent orientation during odor. Based on connectome data, develop computational model showing can promote goal such as an source. Our results suggest processed by separate pathways within goal-directed

Language: Английский

Citations

91
Causation in neuroscience: keeping mechanism meaningful DOI
Lauren N. Ross,

Dani S. Bassett

Nature reviews. Neuroscience, Journal Year: 2024, Volume and Issue: 25(2), P. 81 - 90

Published: Jan. 11, 2024

Language: Английский

Citations

37
Common principles for odour coding across vertebrates and invertebrates DOI

Kara A Fulton,

David Zimmerman,

Aravi Samuel

et al.

Nature reviews. Neuroscience, Journal Year: 2024, Volume and Issue: 25(7), P. 453 - 472

Published: May 28, 2024

Language: Английский

Citations

18
A neural circuit architecture for rapid learning in goal-directed navigation DOI Creative Commons
Chuntao Dan, Brad K. Hulse,

Ramya Kappagantula

et al.

Neuron, Journal Year: 2024, Volume and Issue: 112(15), P. 2581 - 2599.e23

Published: May 24, 2024

Anchoring goals to spatial representations enables flexible navigation but is challenging in novel environments when both must be acquired simultaneously. We propose a framework for how Drosophila uses internal of head direction (HD) build goal upon selective thermal reinforcement. show that flies use stochastically generated fixations and directed saccades express heading preferences an operant visual learning paradigm HD neurons are required modify these based on used symmetric setting expose flies' co-evolve the reliability interacting impacts behavior. Finally, we describe rapid new headings may rest behavioral policy whose parameters form genetically encoded circuit architecture. Such evolutionarily structured architectures, which enable rapidly adaptive behavior driven by representations, relevant across species.

Language: Английский

Citations

17
Connectomics and function of a memory network: the mushroom body of larval Drosophila DOI
Andreas S. Thum, Bertram Gerber

Current Opinion in Neurobiology, Journal Year: 2018, Volume and Issue: 54, P. 146 - 154

Published: Oct. 24, 2018

Language: Английский

Citations

86
Input Connectivity Reveals Additional Heterogeneity of Dopaminergic Reinforcement in Drosophila DOI Creative Commons
Nils Otto, Markus W. Pleijzier,

Isabel C. Morgan

et al.

Current Biology, Journal Year: 2020, Volume and Issue: 30(16), P. 3200 - 3211.e8

Published: July 2, 2020

Different types of Drosophila dopaminergic neurons (DANs) reinforce memories unique valence and provide state-dependent motivational control [1]. Prior studies suggest that the compartment architecture mushroom body (MB) is relevant resolution for distinct DAN functions [2, 3]. Here we used a recent electron microscope volume fly brain [4] to reconstruct fine anatomy individual DANs within three MB compartments. We find 20 γ5 compartment, at least some which reward teaching signals, can be clustered into 5 anatomical subtypes innervate different regions γ5. Reconstructing 821 upstream reveals input selectivity, supporting functional relevance sub-classification. Only one PAM-γ5 subtype γ5(fb) receives direct recurrent feedback from γ5β'2a output (MBONs) behavioral experiments distinguish role these in memory revaluation those reinforcing sugar memory. Other receive major, potentially reinforcing, inputs putative gustatory interneurons or lateral horn neurons, also relay indirect MBONs. similarly reconstructed single aversively PPL1-γ1pedc DAN. The γ1pedc mostly differ they cluster onto dendritic branches, presumably separating its established roles aversive reinforcement appetitive motivation [5, 6]. Tracing identified broad γ5, β'2a, DANs, suggesting distributed populations coordinately regulated. These connectomic analyses therefore reveal further complexity circuits between

Language: Английский

Citations

76
Glial glucose fuels the neuronal pentose phosphate pathway for long-term memory DOI Creative Commons

Éloïse de Tredern,

Yasmine Rabah,

Laure Pasquer

et al.

Cell Reports, Journal Year: 2021, Volume and Issue: 36(8), P. 109620 - 109620

Published: Aug. 1, 2021

Brain function relies almost solely on glucose as an energy substrate. The main model of brain metabolism proposes that is taken up and converted into lactate by astrocytes to fuel the energy-demanding neuronal activity underlying plasticity memory. Whether direct uptake required for memory formation remains elusive. We uncover, in Drosophila, a mechanism shuttling neurons from cortex glia, exclusively perisomatic glial subtype, upon olfactory long-term (LTM). In vivo imaging reveals that, downstream cholinergic activation autocrine insulin signaling increases concentration glia. Glucose then transferred glia somata center pentose phosphate pathway allow LTM formation. contrast, our results indicate increase metabolism, although crucial formation, not routed glycolysis.

Language: Английский

Citations

56
Endocrine cybernetics: neuropeptides as molecular switches in behavioural decisions DOI Creative Commons
Dick R. Nässel, Meet Zandawala

Open Biology, Journal Year: 2022, Volume and Issue: 12(7)

Published: July 1, 2022

Plasticity in animal behaviour relies on the ability to integrate external and internal cues from changing environment hence modulate activity synaptic circuits of brain. This context-dependent neuromodulation is largely based non-synaptic signalling with neuropeptides. Here, we describe select peptidergic systems Drosophila brain that act at different levels a hierarchy associated physiology. These regions, such as central complex mushroom bodies, which supervise specific behaviours. At top level there are small numbers large neurons arborize widely multiple areas orchestrate or global state manner. bottom local provide executive sensory gain intrinsically restricted parts neuronal circuits. The orchestrating receive interoceptive signals mediate energy sleep homeostasis, metabolic circadian timing, well affect food search, aggression mating. Some these can be triggers conflicting behaviours mating versus aggression, feeding, participate circuits, enabling choices switches.

Language: Английский

Citations

55