Diversity of visual inputs to Kenyon cells of the Drosophila mushroom body DOI Creative Commons
Ishani Ganguly, Emily L. Heckman, Ashok Litwin-Kumar

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: July 7, 2024

Abstract The arthropod mushroom body is well-studied as an expansion layer representing olfactory stimuli and linking them to contingent events. However, 8% of Kenyon cells in Drosophila melanogaster receive predominantly visual input, their function remains unclear. Here, we identify inputs using the FlyWire adult whole-brain connectome. Input repertoires are similar across hemispheres connectomes with certain highly overrepresented. Many neurons presynaptic have large receptive fields, while interneuron spatially restricted signals that may be tuned specific features. Individual randomly sample sparse from combinations channels, including multiple optic lobe neuropils. These connectivity patterns suggest coding body, like coding, sparse, distributed, combinatorial. input repertoire smaller population suggests a constrained encoding stimuli.

Language: Английский

Connectomic reconstruction of a female Drosophila ventral nerve cord DOI
Anthony W. Azevedo, Ellen Lesser, Jasper S. Phelps

et al.

Nature, Journal Year: 2024, Volume and Issue: 631(8020), P. 360 - 368

Published: June 26, 2024

Language: Английский

Citations

30
NeuronBridge: an intuitive web application for neuronal morphology search across large data sets DOI Creative Commons
Jody Clements, Cristian Goina, Philip M. Hubbard

et al.

BMC Bioinformatics, Journal Year: 2024, Volume and Issue: 25(1)

Published: March 15, 2024

Abstract Background Neuroscience research in Drosophila is benefiting from large-scale connectomics efforts using electron microscopy (EM) to reveal all the neurons a brain and their connections. To exploit this knowledge base, researchers relate connectome’s structure neuronal function, often by studying individual neuron cell types. Vast libraries of fly driver lines expressing fluorescent reporter genes sets have been created imaged confocal light (LM), enabling targeting for experimentation. However, creating line driving gene expression within single found an EM connectome remains challenge, as it typically requires identifying pair where only interest expressed both. This task other emerging scientific workflows require finding similar across large data different modalities. Results Here, we present NeuronBridge, web application easily rapidly putative morphological matches between We describe functionality construction NeuronBridge service, including its user-friendly graphical user interface (GUI), extensible model, serverless cloud architecture, massively parallel image search engine. Conclusions fills critical gap workflow used hundreds neuroscience around world. offer our software code, open APIs, processed integration reuse, provide service at http://neuronbridge.janelia.org .

Language: Английский

Citations

24
Connectome-constrained networks predict neural activity across the fly visual system DOI Creative Commons
Janne K. Lappalainen, Fabian Tschopp, Sridhama Prakhya

et al.

Nature, Journal Year: 2024, Volume and Issue: 634(8036), P. 1132 - 1140

Published: Sept. 11, 2024

Language: Английский

Citations

23
Common principles for odour coding across vertebrates and invertebrates DOI

Kara A Fulton,

David Zimmerman,

Aravi Samuel

et al.

Nature reviews. Neuroscience, Journal Year: 2024, Volume and Issue: 25(7), P. 453 - 472

Published: May 28, 2024

Language: Английский

Citations

18
Descending control and regulation of spontaneous flight turns in Drosophila DOI
Ivo G. Ros, Jaison J. Omoto, Michael H. Dickinson

et al.

Current Biology, Journal Year: 2024, Volume and Issue: 34(3), P. 531 - 540.e5

Published: Jan. 15, 2024

Language: Английский

Citations

16
Neural circuit mechanisms for steering control in walkingDrosophila DOI Creative Commons

Aleksandr Rayshubskiy,

Stephen L. Holtz,

Alexander Shakeel Bates

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2020, Volume and Issue: unknown

Published: April 5, 2020

Abstract Orienting behaviors provide a continuous stream of information about an organism’s sensory experiences and plans. Thus, to study the links between sensation action, it is useful identify neurons in brain that control orienting behaviors. Here we describe descending Drosophila predict influence orientation (heading) during walking. We show these cells have specialized functions: whereas one cell type predicts sustained low-gain steering, other transient high-gain steering. These latter integrate internally-directed steering signals from head direction system with stimulus-directed multimodal pathways. The inputs are organized produce “see-saw” commands, so increasing output hemisphere accompanied by decreasing hemisphere. Together, our results internal external drives integrated motor commands different timescales, for flexible precise space.

Language: Английский

Citations

72
Systematic annotation of a complete adult maleDrosophilanerve cord connectome reveals principles of functional organisation DOI Creative Commons
Elizabeth C. Marin, Billy J Morris, Tomke Stürner

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: June 6, 2023

Summary Nervous systems function as ensembles of neurons communicating via synaptic connections, and a functional understanding nervous requires extensive knowledge their connectomes. In companion paper (Takemura et al., 2023), we describe the acquisition complete fruit fly nerve cord connectome, first for an animal that can walk or fly. Here, to efficiently navigate appreciate biological significance this categorise name nearly all systematically link them experimental literature. We employ system hierarchical coarse annotations group similar across midline segments, then define systematic cell types sensory neurons, intrinsic ascending non-motor efferent neurons. Stereotyped arrays neuroblasts generate related neuron populations called hemilineages repeat segments cord. confirm larval-born from given hemilineage generally express same neurotransmitter but find earlier born often different one. match over 35% intrinsic, ascending, defining serial sets which were crucial typing motor assign modality 5000 cluster by connectivity, identify serially homologous layered organisation likely corresponding peripheral topography. Finally, present selected examples circuits predicated on programmatic analysis VNC connectome. Our are critical analysing structure descending input output, both described in third (Cheong 2023). These being released part neuprint.janelia.org clio.janelia.org web applications also serve basis connectome through dedicated tools paper.

Language: Английский

Citations

29
Synaptic connectome of theDrosophilacircadian clock DOI Creative Commons
Nils Reinhard,

Ayumi Fukuda,

Giulia Manoli

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 13, 2023

The circadian clock and its output pathways play a pivotal role in optimizing daily processes. To obtain novel insights into how diverse rhythmic physiology behaviors are orchestrated, we have generated the first comprehensive connectivity map of an animal using Drosophila FlyWire brain connectome. Intriguingly, identified additional dorsal neurons, thus showing that network contains ∼240 instead 150 neurons. We also revealed extensive contralateral synaptic within discovered indirect light input to Interestingly, observed sparse monosynaptic between neurons down-stream higher-order centers neurosecretory cells known regulate behavior physiology. Therefore, integrated single-cell transcriptomics receptor mapping decipher putative paracrine peptidergic signaling by Our analyses neuropeptides expressed suggest significantly enriches interconnectivity network.

Language: Английский

Citations

22
Descending networks transform command signals into population motor control DOI Creative Commons
Jonas Braun, Femke Hurtak, Sibo Wang

et al.

Nature, Journal Year: 2024, Volume and Issue: 630(8017), P. 686 - 694

Published: June 5, 2024

Abstract To convert intentions into actions, movement instructions must pass from the brain to downstream motor circuits through descending neurons (DNs). These include small sets of command-like that are sufficient drive behaviours 1 —the circuit mechanisms for which remain unclear. Here we show DNs in Drosophila directly recruit networks additional orchestrate require active control numerous body parts. Specifically, found previously thought alone 2–4 fact co-activate larger populations DNs. Connectome analyses and experimental manipulations revealed this functional recruitment can be explained by direct excitatory connections between interconnected brain. Descending population is necessary behavioural control: with many partners network co-activation complete only simple stereotyped movements their absence. DN reside within behaviour-specific clusters inhibit one another. results support a mechanism generated increasingly large compose combining multiple subroutines.

Language: Английский

Citations

15
Whole-body simulation of realistic fruit fly locomotion with deep reinforcement learning DOI Creative Commons

Roman Vaxenburg,

Igor Siwanowicz,

Josh Merel

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 14, 2024

Abstract The body of an animal influences how the nervous system produces behavior. Therefore, detailed modeling neural control sensorimotor behavior requires a model body. Here we contribute anatomically-detailed biomechanical whole-body fruit fly Drosophila melanogaster in MuJoCo physics engine. Our is general-purpose, enabling simulation diverse behaviors, both on land and air. We demonstrate generality our by simulating realistic locomotion, flight walking. To support these have extended with phenomenological models fluid forces adhesion forces. Through data-driven end-to-end reinforcement learning, that advances enable training network controllers capable locomotion along complex trajectories based high-level steering signals. use visual sensors re-use pre-trained general-purpose controller to perform visually guided tasks. project open-source platform for embodied context.

Language: Английский

Citations

14