Green Synthesis of Zinc Oxide Nanoparticles Using an Aqueous Extract of Punica granatum for Antimicrobial and Catalytic Activity

Journal of Functional Biomaterials, Journal Year: 2023, Volume and Issue: 14(4), P. 205 - 205

Published: April 7, 2023

The peel aqueous extract of Punica granatum was utilized to fabricate zinc oxide nanoparticles (ZnO-NPs) as a green approach. synthesized NPs were characterized by UV-Vis spectroscopy, Fourier transform infrared (FT-IR), X-ray diffraction (XRD), transmission electron microscopy (TEM), and scanning microscopy, which attached an energy dispersive (SEM-EDX). Spherical, well arranged, crystallographic structures ZnO-NPs formed with sizes 10-45 nm. biological activities ZnO-NPs, including antimicrobial catalytic activity for methylene blue dye, assessed. Data analysis showed that the against pathogenic Gram-positive Gram-negative bacteria, unicellular fungi, observed occur in dose-dependent manner, displaying varied inhibition zones low minimum inhibitory concentration (MIC) values ranges 6.25-12.5 µg mL-1. degradation efficacy (MB) using is dependent on nano-catalyst concentration, contact time, incubation condition (UV-light emission). maximum MB percentages 93.4 ± 0.2% attained at 20 mL-1 after 210 min presence UV-light. there no significant difference between 210, 1440, 1800 min. Moreover, high stability degrade five cycles decreasing 4%. Overall, P. granatum-based are promising tools inhibit growth microbes UV-light emission.

Language: Английский

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Biosynthesis of Metal and Metal Oxide Nanoparticles Using Microbial Cultures: Mechanisms, Antimicrobial Activity and Applications to Cultural Heritage

Microorganisms, Journal Year: 2023, Volume and Issue: 11(2), P. 378 - 378

Published: Feb. 2, 2023

Nanoparticles (1 to 100 nm) have unique physical and chemical properties, which makes them suitable for application in a vast range of scientific technological fields. In particular, metal nanoparticle (MNPs) research has been showing promising antimicrobial activities, paving the way new applications. However, despite some into their potential, mechanisms are still not well determined. Nanoparticles' biosynthesis, using plant extracts or microorganisms, shown results as green alternatives synthesis; however, knowledge regarding behind it is neither abundant nor consensual. this review, findings from studies on biosynthesis MNPs were compiled evidence-based proposed. The first revealed importance enzymatic disturbance by internalized ions, while second illustrated role reducing negatively charged molecules. Additionally, main recent (2018-2022) microorganisms summarized analyzed, evidencing prevalence silver nanoparticles synthesized bacteria aiming toward testing potential. Finally, synopsis applied cultural heritage materials showed potential future use preservation.

Language: Английский

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Emerging trends and promising prospects in nanotechnology for improved remediation of wastewater contaminants: Present and future outlooks

Environmental Nanotechnology Monitoring & Management, Journal Year: 2024, Volume and Issue: 21, P. 100913 - 100913

Published: Jan. 8, 2024

Language: Английский

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Antimicrobial and In Vitro Cytotoxic Efficacy of Biogenic Silver Nanoparticles (Ag-NPs) Fabricated by Callus Extract of Solanum incanum L.

Biomolecules, Journal Year: 2021, Volume and Issue: 11(3), P. 341 - 341

Published: Feb. 24, 2021

The in vitro callus induction of Solanum incanum L. was executed on MS medium supplemented with different concentrations auxin and cytokinin utilizing petioles explants leaves. highest significant fresh weights from leaf were 4.68 5.13 g/jar for the with1.0 mg L−1 BA 1.0 2,4-D. extract leaves used green synthesis silver nanoparticles (Ag-NPs). Analytical methods Ag-NPs characterization UV-vis spectroscopy, Fourier Transform Infrared spectroscopy (FT-IR), X-ray diffraction (XRD), Transmission Electron Microscopy (TEM). Spherical, crystallographic sizes ranging 15 to 60nm successfully formed. FT-IR spectra exhibited role metabolites involved reducing capping Ag-NPs. biological activities dose-dependent. MIC value Staphylococcus aureus, Bacillus subtilis, Escherichia coli 12.5 µg mL−1, while it 6.25 mL−1 Klebsiella pneumoniae, Pseudomonas aeruginosa, Candida albicans. inhibition phytopathogenic fungi Alternaria alternata, Fusarium oxysporum, Aspergillus niger, Pythium ultimum 76.3 ± 3.7, 88.9 4.1, 67.8 2.1, 76.4 1.0%, respectively at 200 mL−1. Moreover, synthesized showed cytotoxic efficacy against cancerous cell lines HepG2, MCF-7 normal Vero line IC50 values 21.76 0.56, 50.19 1.71, 129.9 0.94 respectively.

Language: Английский

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Biological Treatment of Real Textile Effluent Using Aspergillus flavus and Fusarium oxysporium and Their Consortium along with the Evaluation of Their Phytotoxicity

Journal of Fungi, Journal Year: 2021, Volume and Issue: 7(3), P. 193 - 193

Published: March 9, 2021

Twenty-one fungal strains were isolated from dye-contaminated soil; out of them, two A2 and G2-1 showed the highest decolorization capacity for real textile effluent were, hence, identified as Aspergillus flavus Fusarium oxysporium based on morphological molecular methods. The percentage 78.12 ± 2.1% was attained in biotreatment with consortium followed by A. F. separately removal percentages 54.68 1.2% 52.41 1.0%, respectively. Additionally, ultraviolet-visible spectroscopy treated that a maximum peak (λmax) 415 nm reduced compared control. indicators wastewater treatment efficacy, namely total dissolved solids, suspended conductivity, biological oxygen demand, chemical demand 78.2, 78.4, 58.2, 78.1, 77.6%, respectively, demonstrated considerable decrease values due to treatment. reduction mass area along appearance new peaks GC-MS confirms successful biodegradation process. toxicity effluents seed germination Vicia faba decreased shoot length after irrigation 15.12 1.01 cm tap-water, which 17.8 0.7 cm. Finally, we recommended excessive uses synthetic dyes utilized approaches reuse uneaten plants especially water scarcity worldwide.

Language: Английский

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Synthesis of Eco-Friendly Biopolymer, Alginate-Chitosan Composite to Adsorb the Heavy Metals, Cd(II) and Pb(II) from Contaminated Effluents

Materials, Journal Year: 2021, Volume and Issue: 14(9), P. 2189 - 2189

Published: April 24, 2021

Efficient removal of Cd(II) and Pb(II) from contaminated water is considered a fundamental point view. Synthetic hydrogel biopolymers based on chitosan alginate (cost-effective eco-friendly) were successfully designed characterized by highly efficient contaminants. The sorbents are FTIR, SEM-EDX, TGA, XPS analyses textural properties which qualified N2 adsorption. sorption firstly investigated the effect pH, isotherms, uptake kinetics, selectivity multi-metal solution with equi-molar concentration. sorbent 1:3 ratios (of respectively) most effective for metal (i.e., 0.81 mmol Cd g−1 0.41 Pb g−1). Langmuir Sip’s models fitted better adsorption isotherms compared to Freundlich model. Uptake kinetics was well pseudo-first-order rate equation, while saturation achieved within 40 min. shows good reproducibility through duplicate experiments negligible decreasing efficiency (>2.5%). applied treatment samples collected industrial area 653 203 times over MCL respectively according WHO). concentration drastically decreased in effluents as pH increased up 99% both elements at 5.8 SD equivalent 1 g L−1 5 h.

Language: Английский

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Mycosynthesis of Hematite (α-Fe2O3) Nanoparticles Using Aspergillus niger and Their Antimicrobial and Photocatalytic Activities

Bioengineering, Journal Year: 2022, Volume and Issue: 9(8), P. 397 - 397

Published: Aug. 17, 2022

Nanoparticles (NPs) and nanomaterials (NMs) are now widely used in a variety of applications, including medicine, solar energy, drug delivery, water treatment, pollution detection. Hematite (α-Fe2O3) nanoparticles (Hem-NPs) were manufactured this work by utilizing cost-effective ecofriendly approach that included biomass filtrate A. niger AH1 as bio-reducer. The structural optical properties Hem-NPs investigated using X-ray diffraction (XRD), transmission electron microscopy (TEM), dynamic light scattering (DLS), UV-visible Fourier-transform infrared (FTIR) spectroscopies. results revealed all the studied parameters, well their interactions, had significant impact on crystallite size. average diameter size biosynthesized ranged between 60 80 nm. antimicrobial photocatalytic activities investigated. exhibited antibacterial activity against E. coli, B. subtilis, S. mutans with MICs 125, 31.25, 15.62 µg/mL, respectively. Moreover, antifungal C. albicans fumigatus, where 2000 62.5 efficiency was determined for rapid biodegradation crystal violet (CV) dye, reaching up to 97 percent after 150 min. Furthermore, successfully more than once regarded its efficacy. In conclusion, demonstrated both CV dye.

Language: Английский

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The Potency of Fungal-Fabricated Selenium Nanoparticles to Improve the Growth Performance of Helianthus annuus L. and Control of Cutworm Agrotis ipsilon

Catalysts, Journal Year: 2021, Volume and Issue: 11(12), P. 1551 - 1551

Published: Dec. 19, 2021

The application of green nanotechnology in agriculture has been receiving substantial attention, especially the development new nano-fertilizers and nano-insecticides. Herein, metabolites secreted by fungal strain Penicillium chrysogenum are used as a reducing agent for selenium ions to form nanoparticles (Se-NPs). synthesized Se-NPs were characterized using color change, UV-Vis spectroscopy, Fourier transform infrared (FT-IR) transmission electron microscopy (TEM), energy dispersive X-ray (EDX), diffraction (XRD), dynamic light scattering (DLS). biomass filtrate changed from colorless ruby red after mixing with sodium selenite maximum surface plasmon resonance at 262 nm. Data exhibits successful formation spherical, amorphous sizes ranging between 3–15 nm weight percentage 38.52%. efficacy on growth performance sunflower (Helianthus annuus L.) inhibition cutworm Agrotis ipsilon was investigated. field experiment revealed potentiality enhance parameters carotenoid content sunflower, 20 ppm. chlorophylls, carbohydrates, proteins, phenolic compounds, free proline contents markedly promoted response concentrations. antioxidant enzymes (peroxidase, catalase, superoxide dismutase, polyphenol oxidase) significantly decreased compared control. analysis showed that highest mortality 1st, 2nd, 3rd, 4th, 5th instar larvae achieved 25 ppm percentages 89.7 ± 0.3, 78.3 72.3 0.6, 63.7 68.7 0.3 respectively 72 h.

Language: Английский

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An Eco-Friendly Approach to the Control of Pathogenic Microbes and Anopheles stephensi Malarial Vector Using Magnesium Oxide Nanoparticles (Mg-NPs) Fabricated by Penicillium chrysogenum

International Journal of Molecular Sciences, Journal Year: 2021, Volume and Issue: 22(10), P. 5096 - 5096

Published: May 12, 2021

The discovery of eco-friendly, rapid, and cost-effective compounds to control diseases caused by microbes insects are the main challenges. Herein, magnesium oxide nanoparticles (MgO-NPs) successfully fabricated harnessing metabolites secreted Penicillium chrysogenum. MgO-NPs were characterized using UV-Vis, XRD, TEM, DLS, EDX, FT-IR, XPS analyses. Data showed successful formation crystallographic, spherical, well-dispersed with sizes 7–40 nm at a maximum wavelength 250 nm. EDX analysis confirms presence Mg O ions as components weight percentages 13.62% 7.76%, respectively. activity an antimicrobial agent was investigated against pathogens Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, Escherichia coli, Candida albicans, exhibited zone inhibitions 12.0 ± 0.0, 12.7 0.9, 23.3 0.8, 17.7 1.6, 14.7 0.6 mm respectively, 200 µg mL−1. is decreased decreasing concentration. biogenic exhibit high efficacy different larvae instar pupa Anopheles stephensi, LC50 values 12.5–15.5 ppm for I–IV 16.5 pupa. Additionally, 5 mg/cm2 highest protection adults 100% 150 min 67.6% 1.4% 210 min.

Language: Английский

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Aspergillus flavus-Mediated Green Synthesis of Silver Nanoparticles and Evaluation of Their Antibacterial, Anti-Candida, Acaricides, and Photocatalytic Activities

Catalysts, Journal Year: 2022, Volume and Issue: 12(5), P. 462 - 462

Published: April 21, 2022

Aspergillus flavus F5 was used to reduce AgNO3 form silver nanoparticles (Ag-NPs) that were monitored by a color change from colorless yellowish-brown. The characterizations achieved UV-Vis spectroscopy, FT-IR, TEM, SEM-EDX, and XRD. Data showed there successful formation of crystalline, spherical shape Ag-NPs with particle average size 12.5 ± 5.1 nm. FT-IR clarified the role various functional groups in reducing/capping process. EDX-SEM revealed main component as-formed sample set be mainly Ag weight percentage 46.1%. synthesized exhibit antibacterial anti-Candida activity against Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, Escherichia coli, Candida albicans, C. glabrata, tropicalis, parapsilosis, inhibition zones ranging between 9.3 0.5 20.8 0.3 nm based on concentrations MIC values 6.25 25 ppm. mortality percentages Tyrophagus putrescentiae mite species due mixing their diet different 0.5, 1.0, 1.5 mg 55.7 2.1, 73.3 1.5, 87.4 1.6% respectively after 20 days post-treatment. catalytic degrade methylene blue (MB) investigated presence absence light irradiation. high photocatalytic degradation MB compared dark conditions at times concentrations. At concentration 70 mg/30 mL 200 min., dye removal 86.4 0.4% irradiation versus 66.5 1.1% conditions.

Language: Английский

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