Juglone-Bearing Thiopyrano[2,3-d]thiazoles Induce Apoptosis in Colorectal Adenocarcinoma Cells DOI Creative Commons
Yuliia Kozak, Nataliya Finiuk, Robert Czarnomysy

et al.

Cells, Journal Year: 2025, Volume and Issue: 14(6), P. 465 - 465

Published: March 20, 2025

Colorectal cancer is a major global health challenge, with current treatments limited by toxicity and resistance. Thiazole derivatives, known for their bioactivity, are emerging as promising alternatives. Juglone (5-hydroxy-1,4-naphthoquinone) naturally occurring compound anticancer properties, its incorporation into thiopyrano[2,3-d]thiazole scaffolds may enhance therapeutic potential. This study examined the cytotoxicity of thiopyrano[2,3-d]thiazoles effects on apoptosis in colorectal cells. Les-6547 Les-6557 increased population ROS-positive HT-29 cells approximately 10-fold compared control (36.3% 38.5% vs. 3.8%, respectively), potentially contributing to various downstream effects. Elevated ROS levels were associated cell cycle arrest, inhibition DNA biosynthesis, reduced proliferation. A significant shift distribution was observed, an increase S-phase (from 17.3% 34.7% 51.3% Les-6557, respectively) G2/M phase 24.3% 39.9% 28.8%). Additionally, inhibited biosynthesis cells, IC50 values 2.21 µM 2.91 µM, respectively. generation initiate intrinsic apoptotic pathway. activated both extrinsic pathways, demonstrated notable increases activity caspase 3/7, 8, 9, 10. provides robust basis investigating detailed molecular mechanisms action potential Les-6557.

Language: Английский

c-Fos regulated by TMPO/ERK axis promotes 5-FU resistance via inducing NANOG transcription in colon cancer DOI Creative Commons
Yanping Gui, Xiaoping Qian,

Youxiang Ding

et al.

Cell Death and Disease, Journal Year: 2024, Volume and Issue: 15(1)

Published: Jan. 17, 2024

Abstract Acquired drug resistance is one of the most common limitations for clinical response colon cancer to 5-Fluorouracil (5-FU)-based chemotherapy. The relevant molecular mechanisms might be diversity, but still not elucidated clearly. In this study, we aimed investigate potential c-Fos, a subfamily activator protein-1, in 5-FU chemoresistance. We determined that phosphorylated c-Fos promoted cells by facilitating stemness. Mechanically, treatment induced autolysosome-dependent degradation TMPO, which subsequently triggered ERK-mediated phosphorylation c-Fos. Additionally, was found bind promoter NANOG and at Ser 374 required its regulation expression. ablation impaired c-Fos/p-c-Fos Taken together, these findings revealed TMPO-mediated conferred regulating expression promoting cell stemness cells. could as therapeutic target cancer.

Language: Английский

Citations

15
Cell Immortality: In Vitro Effective Techniques to Achieve and Investigate Its Applications and Challenges DOI Creative Commons

Mahla Chalak,

Mahdi Hesaraki, Seyedeh Nasim Mirbahari

et al.

Life, Journal Year: 2024, Volume and Issue: 14(3), P. 417 - 417

Published: March 21, 2024

Cells are very important to researchers due their use in various biological studies vitro and vivo settings. This importance stems from the short lifespan of most cells under laboratory conditions, which can pose significant challenges, such as difficulties associated with extraction source tissue, ethical concerns about separating human or animal models, limited cell passage ability, variation results differences obtained cells, among other issues. In general, conditions divide into a number, known Hayflick limit, telomere erosion at end each cellular cycle. Given this problem, require lines that do not enter senescence phase after number divisions. allow for more stable over time, prevent laborious work separation repeated cultivation, save time money research projects. The aim review is summarize function effect immortalization techniques, methods, advantages disadvantages, ultimately application line production fields.

Language: Английский

Citations

15
Targeted Treatment against Cancer Stem Cells in Colorectal Cancer DOI Open Access
Julia Martínez-Pérez, C. Sevillano, Maria A Dominguez-Cejudo

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(11), P. 6220 - 6220

Published: June 5, 2024

The cancer stem cell (SC) theory proposes that a population of SCs serves as the driving force behind fundamental tumor processes, including metastasis, recurrence, and resistance to therapy. standard care for patients with stage III high-risk II colorectal (CRC) includes surgery adjuvant chemotherapy. Fluoropyrimidines their combination oxaliplatin increased cure rates, being able eradicate occult metastatic SC in fraction patients. treatment unresectable CRC is based on chemotherapy, antibodies VEGF EGFR, tyrosine-kinase inhibitors. Immunotherapy used MSI-H tumors. Currently drugs target dividing cells and, while often effective at debulking mass, these agents have largely failed disease. are generated either due genetic epigenetic alterations stem/progenitor or dedifferentiation somatic where diverse signaling pathways such Wnt/β-catenin, Hedgehog, Notch, TGF-β/SMAD, PI3K/Akt/mTOR, NF-κB, JAK/STAT, DNA damage response, Hippo-YAP play key role. Anti-neoplastic treatments could be improved by elimination SCs, becoming an attractive design novel agents. Here, we present review clinical trials assessing efficacy targeted focusing CRC.

Language: Английский

Citations

10
Lobaric acid suppresses the stemness potential of colorectal cancer cells through mTOR/AKT DOI
Sultan Pulat,

Jaycee Augusto Gumiran Paguiri,

Chathurika D. B. Gamage

et al.

BioFactors, Journal Year: 2025, Volume and Issue: 51(1)

Published: Jan. 1, 2025

Abstract Stereocaulon alpinum has been found to have potential pharmaceutical properties due the presence of secondary metabolites such as usnic acid, atranorin, and lobaric acid (LA) which anticancer activity. On other hand, effect LA on stemness colorectal cancer (CRC) cells remains unexplored, not yet thoroughly investigated. In this study, we examined inhibitory activity from against CRC investigated possible underlying mechanisms. The results demonstrated that did inhibit cell viability CSC221 DLD1. addition, significantly decreased spheroid formation Moreover, treatment suppressed stem (CSC) markers; aldehyde dehydrogenase 1 (ALDH1), B‐cell‐specific Moloney leukemia virus insertion site (BMI1), musashi1 (MSI1), leucine‐rich repeat‐containing G‐protein coupled receptor 5 (Lgr5), along with sonic hedgehog (Shh) mTOR/AKT pathways contribute maintaining cells. Therefore, may be a new therapeutic approach for reducing

Language: Английский

Citations

1
The Impact of Cancer Stem Cells in Colorectal Cancer DOI Open Access
Petru Radu, Mihai Zurzu, Anca Tigora

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(8), P. 4140 - 4140

Published: April 9, 2024

Despite incessant research, colorectal cancer (CRC) is still one of the most common causes fatality in both men and women worldwide. Over time, advancements medical treatments have notably enhanced survival rates patients with cancer. Managing metastatic CRC involves a complex tradeoff between potential benefits adverse effects treatment, considering factors like disease progression, treatment toxicity, drug resistance, overall impact on patient's quality life. An increasing body evidence highlights significance stem cell (CSC) concept, proposing that CSCs occupy central role triggering been focal point extensive research variety types, including CRC. Colorectal cells (CCSCs) play crucial tumor initiation, metastasis, therapy making them targets. Various methods exist for isolating CCSCs, understanding mechanisms resistance associated crucial. This paper offers an overview current pertaining to comprehension

Language: Английский

Citations

7
Focused Ultrasound as Targeted Therapy for Colorectal Cancer: A Comprehensive Review DOI Creative Commons
Muhammad Awais Farooqi, Mahnoor Mahnoor, Kaylee Marie Delgado

et al.

Gastrointestinal Disorders, Journal Year: 2024, Volume and Issue: 6(2), P. 380 - 401

Published: April 13, 2024

Traditional cancer treatments have not significantly improved the survival rates for individuals with colorectal cancer. As a result, there is dire need to explore novel treatment modalities that can target cancer-specific niches, transform cold tumors into hot ones, and disrupt tumor niche. Therapeutic focused ultrasound, recognized its capacity induce thermal mechanical impacts on tissue, potentially eliminate cells elicit body’s anticancer reaction by disrupting microenvironment. This article provides an overview of recent developments in employing therapeutic ultrasound (TFUS) enhance natural defenses against cancers. It also discusses studies examining utility TFUS treating patients research indicating potential stimulate response various vitro vivo models. Furthermore, it explores effects immune system highlights safety effectiveness managing cancer, providing relief from pain, improving rates. Given indications may bolster augment therapy clinical preclinical models, has emerge as pivotal tool settings.

Language: Английский

Citations

6
Recent advances in nanomedicine preparative methods and their therapeutic potential for colorectal cancer: a critical review DOI Creative Commons
Arinjay Jain, Sankha Bhattacharya

Frontiers in Oncology, Journal Year: 2023, Volume and Issue: 13

Published: June 22, 2023

Colorectal cancer (CRC) is a prevalent malignancy that affects large percentage of the global population. The conventional treatments for CRC have number limitations. Nanoparticles emerged as promising treatment method due to their ability directly target cells and regulate drug release, thereby enhancing therapeutic efficacy minimizing side effects. This compilation examines use nanoparticles delivery systems treatment. Different nanomaterials can be used administer anticancer drugs, including polymeric nanoparticles, gold liposomes, solid lipid nanoparticles. In addition, we discuss recent developments in nanoparticle preparation techniques, such solvent evaporation, salting-out, ion gelation, nanoprecipitation. These methods demonstrated high penetrating epithelial cells, prerequisite effective delivery. article focuses on various targeting mechanisms utilized by CRC-targeted advancements this field. review offers descriptive information regarding numerous nano-preparative procedures colorectal treatments. We also outlook innovative techniques management CRC, potential application targeted concludes with discussion current nanotechnology patents clinical studies diagnose CRC. results investigation suggest great cancer.

Language: Английский

Citations

16
Cancer Stem Cells and the Tumor Microenvironment in Tumor Drug Resistance DOI

Qiuzhi Gao,

Yixiang Zhan,

Li Sun

et al.

Stem Cell Reviews and Reports, Journal Year: 2023, Volume and Issue: 19(7), P. 2141 - 2154

Published: July 21, 2023

Language: Английский

Citations

15
The endocannabinoid system, a new gatekeeper in the pharmacology of human hepatocellular carcinoma DOI

Nasim Ebrahimi,

Nazanin Pazhouhesh Far,

Siavash Seifollahy Fakhr

et al.

Environmental Research, Journal Year: 2023, Volume and Issue: 228, P. 115914 - 115914

Published: April 14, 2023

Language: Английский

Citations

12
Integration of single-cell sequencing and bulk RNA-seq to identify and develop a prognostic signature related to colorectal cancer stem cells DOI Creative Commons

Jiale Wu,

Wanyu Li,

Junyu Su

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: May 28, 2024

Abstract The prognosis for patients with colorectal cancer (CRC) remains worse than expected due to metastasis, recurrence, and resistance chemotherapy. Colorectal stem cells (CRCSCs) play a vital role in tumor chemotherapy resistance. However, there are currently no prognostic markers based on CRCSCs-related genes available clinical use. In this study, single-cell transcriptome sequencing was employed distinguish (CSCs) the CRC microenvironment analyze their properties at level. Subsequently, data from TCGA GEO databases were utilized develop risk model validate its diagnostic performance. Additionally, functional enrichment, immune response, chemotherapeutic drug sensitivity of relevant investigated. Lastly, key gene RPS17 identified as potential marker therapeutic target further comprehensive studies. Our findings provide new insights into treatment offer novel perspectives systematic understanding development.

Language: Английский

Citations

4