Xanthomonas rydalmerensis sp. nov., a non-pathogenic member of Group 1 Xanthomonas DOI
Daniel J. E. McKnight, Johanna Wong‐Bajracharya, Efenaide B. Okoh

et al.

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Journal Year: 2024, Volume and Issue: 74(3)

Published: March 27, 2024

Five bacterial isolates were isolated from Fragaria × ananassa in 1976 Rydalmere, Australia, during routine biosecurity surveillance. Initially, the results of biochemical characterisation indicated that these represented members genus Xanthomonas . To determine their species, further analysis was conducted using both phenotypic and genotypic approaches. Phenotypic involved MALDI-TOF MS BIOLOG GEN III microplates, which confirmed but did not allow them to be classified with respect species. Genome relatedness indices extensive phylogenetic a novel On basis minimal presence virulence-associated factors typically found genomes , we suggest are non-pathogenic. This conclusion supported by pathogenicity assay. findings, propose name rydalmerensis DAR 34855 T = ICMP 24941 as type strain.

Language: Английский

Rhizosphere bacterial interactions and impact on plant health DOI Creative Commons

Jane Chepsergon,

Lucy Moleleki

Current Opinion in Microbiology, Journal Year: 2023, Volume and Issue: 73, P. 102297 - 102297

Published: March 30, 2023

The rhizosphere is a chemically complex environment that harbors strikingly diverse microbial community. past few decades have seen rapid growth in the body of literature on plant–microbe–microbe interactions and plant health. Thus, aim this paper to review current knowledge (specifically bacteria) how these influence microbiomes impact This article discusses (i) recruits beneficial bacteria ii) competition between mechanisms/weapons employed bacteria–bacteria shapes microbiome turn affects heath. discussion mainly focuses interference competition, characterized by production specialized metabolites (antibacterial compounds) exploitative where bacterial strain restricts competitor's access nutrients such as through secretion siderophores could allude cooperation. Understanding mechanisms plant–bacteria provide insights into manipulate for improved agricultural outcomes.

Language: Английский

Citations

78
Leaf microbiome dysbiosis triggered by T2SS-dependent enzyme secretion from opportunistic Xanthomonas pathogens DOI Creative Commons
Sebastian Pfeilmeier,

Anja Werz,

Marine Ote

et al.

Nature Microbiology, Journal Year: 2024, Volume and Issue: 9(1), P. 136 - 149

Published: Jan. 3, 2024

In healthy plants, the innate immune system contributes to maintenance of microbiota homoeostasis, while disease can be associated with microbiome perturbation or dysbiosis, and enrichment opportunistic plant pathogens like Xanthomonas. It is currently unclear whether change occurs independently caused by latter. Here we tested if protein export through type-2 secretion (T2SS) Xanthomonas causes dysbiosis in Arabidopsis thaliana immunocompromised plants. We found that strains secrete a cocktail cell wall-degrading enzymes promote growth during infection. Disease severity leaf tissue degradation were increased A. mutants lacking NADPH oxidase RBOHD. Experiments gnotobiotic synthetic bacterial communities wild-type T2SS-mutant revealed virulence composition are controlled T2SS. Overall, compromised plants enrich pathogens, which damage tissues ultimately cause facilitating specific commensal bacteria.

Language: Английский

Citations

19
Regulation and inhibition of type III secretion systems in plant pathogenic bacteria DOI Creative Commons
Muhammad Asif, Xin Xie, Zhibo Zhao

et al.

Phytopathology Research, Journal Year: 2025, Volume and Issue: 7(1)

Published: Feb. 20, 2025

Abstract Type III secretion systems (T3SS) are syringe-like apparatuses acting as protein transport nanomachines found in most Gram-negative bacterial pathogens. They can inject effector proteins into the host cell cytoplasm, crossing membrane, and cause infection. Due to their critical role pathogenicity, T3SS represent attractive targets for vaccinations disease treatments. This review elucidates overarching structural framework operational mechanisms of while also delineating responsiveness phytobacterial host-derived signals nuanced orchestration activities by environmental stimuli. discussion encompasses shared features idiosyncratic attributes among a spectrum pathogens, including but not limited Pseudomonas syringae , Ralstonia Xanthomonas Erwinia . Additionally, we scrutinize contribution natural products synthetic chemicals inhibitors, elucidating hallmark ongoing quest design novel drugs. An in-depth comprehension functionality modes action diverse inhibitors holds promise developing innovative drugs aimed at swiftly suppressing phyto-pathogenicity elicited species.

Language: Английский

Citations

1
Advancements in Escherichia coli secretion systems for enhanced recombinant protein production DOI

Sudarsana Reddy Lokireddy,

Sridhar Rao Kunchala,

Ramakrishna Vadde

et al.

World Journal of Microbiology and Biotechnology, Journal Year: 2025, Volume and Issue: 41(3)

Published: March 1, 2025

Language: Английский

Citations

1
Biofilm-Forming Ability of Phytopathogenic Bacteria: A Review of its Involvement in Plant Stress DOI Creative Commons
María Evangelina Carezzano, María Fernanda Paletti Rovey,

Lorena del Rosario Cappellari

et al.

Plants, Journal Year: 2023, Volume and Issue: 12(11), P. 2207 - 2207

Published: June 3, 2023

Phytopathogenic bacteria not only affect crop yield and quality but also the environment. Understanding mechanisms involved in their survival is essential to develop new strategies control plant disease. One such mechanism formation of biofilms; i.e., microbial communities within a three-dimensional structure that offers adaptive advantages, as protection against unfavorable environmental conditions. Biofilm-producing phytopathogenic are difficult manage. They colonize intercellular spaces vascular system host plants cause wide range symptoms necrosis, wilting, leaf spots, blight, soft rot, hyperplasia. This review summarizes up-to-date information about saline drought stress (abiotic stress) then goes on focus biotic produced by biofilm-forming bacteria, which responsible for serious disease many crops. Their characteristics, pathogenesis, virulence factors, systems cellular communication, molecules implicated regulation these processes all covered.

Language: Английский

Citations

22
Bacterial secretion system functions: evidence of interactions and downstream implications DOI
Silindile Maphosa, Lucy Moleleki, Thabiso Motaung

et al.

Microbiology, Journal Year: 2023, Volume and Issue: 169(4)

Published: April 21, 2023

Unprecedented insights into the biology and functions of bacteria have been continue to be gained through studying bacterial secretion systems in isolation. This method, however, results our understanding being primarily based on idea that they operate independently, ignoring subtleties downstream interconnections. Gram-negative are naturally able adapt navigate their frequently varied dynamic surroundings, mostly because covert connections between systems. Therefore, comprehend some linked repercussions for organisms follow this discourse, it is vital mechanistic how intersecretion system rivalry, virulence, survival, among other things. To purpose, paper discusses a few key instances molecular antagonistic interdependent relationships produced functional products.

Language: Английский

Citations

19
Xanthomonas hortorum – beyond gardens: Current taxonomy, genomics, and virulence repertoires DOI Creative Commons
Nay C. Dia, Lucas Morinière, B. G. Cottyn

et al.

Molecular Plant Pathology, Journal Year: 2022, Volume and Issue: 23(5), P. 597 - 621

Published: Jan. 23, 2022

Bacteria; Phylum Proteobacteria; Class Gammaproteobacteria; Order Lysobacterales (earlier synonym of Xanthomonadales); Family Lysobacteraceae Xanthomonadaceae); Genus Xanthomonas; Species X. hortorum; Pathovars: pv. carotae, vitians, hederae, pelargonii, taraxaci, cynarae, and gardneri.

Language: Английский

Citations

28
Complete Genome Assemblies of All Xanthomonas translucens Pathotype Strains Reveal Three Genetically Distinct Clades DOI Creative Commons
Florian Goettelmann, Verónica Román-Reyna, Sébastien Cunnac

et al.

Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 12

Published: March 2, 2022

The Xanthomonas translucens species comprises phytopathogenic bacteria that can cause serious damage to cereals and forage grasses. So far, the genomic resources for X. were limited, which hindered further understanding of host-pathogen interactions at molecular level development disease-resistant cultivars. To this end, we complemented available complete genome sequence pv. pathotype strain DSM 18974 by sequencing genomes all other 10 strains using PacBio long-read technology assembled sequences. Phylogeny based on average nucleotide identity (ANI) revealed three distinct clades within species, propose classify as Xt-I, Xt-II, Xt-III. In addition 2,181 core genes, a total 190, 588, 168 genes found be exclusive each clade, respectively. Moreover, 29 non-transcription activator-like effector (TALE) 21 TALE type III classes found, clade- or strain-specific effectors identified. Further investigation these could help identify are critically involved in pathogenicity and/or host adaptation, setting grounds new resistant

Language: Английский

Citations

25
Surface coatings based on chitosan and tannins applied in the in vivo prevention of corn streak disease DOI
Suelen P. Facchi, Paulo R. Souza, Débora Aparecida de Almeida

et al.

Chemical Engineering Journal, Journal Year: 2023, Volume and Issue: 477, P. 147003 - 147003

Published: Oct. 29, 2023

Language: Английский

Citations

15
Oxytetracycline and Streptomycin Resistance Genes in Xanthomonas arboricola pv. pruni, the Causal Agent of Bacterial Spot in Peach DOI Creative Commons
Austin Herbert, C. Nathan Hancock,

Brodie Cox

et al.

Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 13

Published: Feb. 25, 2022

Xanthomonas arboricola pv. pruni ( Xap ) causes bacterial spot, a major worldwide disease of Prunus species. Very few chemical management options are available for this and frequent applications oxytetracycline (OTC) in the United States peach orchards have raised concerns about resistance development. During 2017–2020, 430 strains were collected from ten South Carolina. Seven OTC-resistant (OTC R found 2017 2020 four 20–270 km apart. Interestingly, seven also resistant to streptomycin (STR). Six grew on media amended with ≤100 μg/mL OTC, while one strain, R1, ≤250 OTC. Genome sequence analysis representative OTC revealed 14–20 kb plasmid carrying tetC , tetR strAB each strain. These three genes transferable perforans via conjugation, they PCR confirmed all strains. When cloned expressed together sensitive transconjugants showed was alone TetC expression inducible by six wild-type However, R1 strain not expressed, possibly due presence Tn 3 gene, case constitutively expressed. data suggest that confers strains, regulates level conferred . To our knowledge, is first report plant pathogenic xanthomonads.

Language: Английский

Citations

22