Campus node-based wastewater surveillance enables COVID-19 case localization and confirms lower SARS-CoV-2 burden relative to the surrounding community DOI Creative Commons
Jangwoo Lee, Nicole Acosta, Barbara Waddell

et al.

Water Research, Journal Year: 2023, Volume and Issue: 244, P. 120469 - 120469

Published: Aug. 8, 2023

Wastewater-based surveillance (WBS) has been established as a powerful tool that can guide health policy at multiple levels of government. However, this approach not well assessed more granular scales, including large work sites such University campuses. Between August 2021 and April 2022, we explored the occurrence SARS-CoV-2 RNA in wastewater using qPCR assays from complimentary sewer catchments residential buildings spanning Calgary's campus how compared to municipal treatment plant servicing campus. Real-time contact tracing data was used evaluate an association between burden clinically confirmed cases assess potential WBS for disease monitoring across worksites. Concentrations N1 N2 varied significantly six sampling - regardless several normalization strategies with certain consistently demonstrating values 1-2 orders higher than others. Relative clinical identified specific sewersheds, provided one-week leading indicator. Additionally, our comprehensive strategy enabled estimation total per capita, which lower surrounding community (p≤0.001). Allele-specific variants were representative large, no time did emerging first debut on This study demonstrates be efficiently applied locate hotspots activity very scale, predict complex

Language: Английский

Municipal and neighbourhood level wastewater surveillance and subtyping of an influenza virus outbreak DOI Creative Commons
Élisabeth Mercier, Patrick M. D’Aoust, Ocean Thakali

et al.

Scientific Reports, Journal Year: 2022, Volume and Issue: 12(1)

Published: Sept. 22, 2022

Recurrent influenza epidemics and pandemic potential are significant risks to global health. Public health authorities use clinical surveillance locate monitor influenza-like cases outbreaks mitigate hospitalizations deaths. Currently, integration of is the only reliable method for reporting types subtypes warn emergent strains. The utility wastewater (WWS) during COVID-19 as a less resource intensive replacement or complement has been predicated on analyzing viral fragments in wastewater. We show here that virus targets stable partitions favorably solids fraction. By quantifying, typing, subtyping municipal primary sludge community outbreak, we forecasted citywide flu outbreak with 17-day lead time provided population-level near real-time feasibility WWS at neighbourhood levels real using minimal resources infrastructure.

Language: Английский

Citations

110
Does normalization of SARS-CoV-2 concentrations by Pepper Mild Mottle Virus improve correlations and lead time between wastewater surveillance and clinical data in Alberta (Canada): comparing twelve SARS-CoV-2 normalization approaches DOI Open Access
Rasha Maal‐Bared, Yuanyuan Qiu, Qiaozhi Li

et al.

The Science of The Total Environment, Journal Year: 2022, Volume and Issue: 856, P. 158964 - 158964

Published: Sept. 24, 2022

Language: Английский

Citations

78
Monitoring influenza and respiratory syncytial virus in wastewater. Beyond COVID-19 DOI Creative Commons
Daniel Toribio-Avedillo, Clara Gómez-Gómez, Laura Sala‐Comorera

et al.

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 892, P. 164495 - 164495

Published: May 26, 2023

Wastewater-based surveillance can be a valuable tool to monitor viral circulation and serve as an early warning system. For respiratory viruses that share similar clinical symptoms, namely SARS-CoV-2, influenza, syncytial virus (RSV), identification in wastewater may allow differentiation between seasonal outbreaks COVID-19 peaks. In this study, these well standard indicators of fecal contamination, weekly sampling campaign was carried out for 15 months (from September 2021 November 2022) two treatment plants the entire population Barcelona (Spain). Samples were concentrated by aluminum hydroxide adsorption-precipitation method then analyzed RNA extraction RT-qPCR. All samples positive while positivity rates influenza RSV significantly lower (10.65 % A (IAV), 0.82 B (IBV), 37.70 RSV-A 34.43 RSV-B). Gene copy concentrations SARS-CoV-2 often approximately 1 2 logarithmic units higher compared other viruses. Clear peaks IAV H3:N2 February March 2022 winter observed, which matched chronological incidence infections recorded Catalan Government database. conclusion, data obtained from provided new information on abundance area correlated favorably with data.

Language: Английский

Citations

62
Wastewater-based epidemiology predicts COVID-19-induced weekly new hospital admissions in over 150 USA counties DOI Creative Commons
Xuan Li, Huan Liu, Li Gao

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: July 28, 2023

Although the coronavirus disease (COVID-19) emergency status is easing, COVID-19 pandemic continues to affect healthcare systems globally. It crucial have a reliable and population-wide prediction tool for estimating COVID-19-induced hospital admissions. We evaluated feasibility of using wastewater-based epidemiology (WBE) predict weekly new hospitalizations in 159 counties across 45 states United States America (USA), covering population nearly 100 million. Using county-level wastewater surveillance data (over 20 months), WBE-based models were established through random forest algorithm. accurately predicted admissions, allowing preparation window 1-4 weeks. In real applications, periodically updated showed good accuracy transferability, with mean absolute error within 4-6 patients/100k upcoming hospitalization numbers. Our study demonstrated potential WBE as an effective method provide early warnings systems.

Language: Английский

Citations

41
A multistate assessment of population normalization factors for wastewater-based epidemiology of COVID-19 DOI Creative Commons
Andrew Rainey,

Song Liang,

Joseph H. Bisesi

et al.

PLoS ONE, Journal Year: 2023, Volume and Issue: 18(4), P. e0284370 - e0284370

Published: April 12, 2023

Wastewater-based epidemiology (WBE) has become a valuable tool for monitoring SARS-CoV-2 infection trends throughout the COVID-19 pandemic. Population biomarkers that measure relative human fecal contribution to normalize wastewater concentrations are needed improved analysis and interpretation of community trends. The Centers Disease Control Prevention National Wastewater Surveillance System (CDC NWSS) recommends using flow rate or indicators as population normalization factors. However, there is no consensus on which factor performs best. In this study, we provided first multistate assessment effects (crAssphage, F+ Coliphage, PMMoV) correlation cases CDC NWSS dataset 182 communities across six U.S. states. Flow normalized produced strongest with cases. from three were significantly lower than rate. Additionally, reverse transcription droplet digital polymerase chain reaction (RT-ddPCR) values over samples analyzed real-time quantitative (rRT-qPCR). Our shows utilizing RT-ddPCR generate between

Language: Английский

Citations

28
Degradation rates influence the ability of composite samples to represent 24-hourly means of SARS-CoV-2 and other microbiological target measures in wastewater DOI Creative Commons
Kristina M. Babler,

Mark Sharkey,

Samantha Abelson

et al.

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 867, P. 161423 - 161423

Published: Jan. 6, 2023

Language: Английский

Citations

25
Detection of the clinically persistent, pathogenic yeast spp. Candida auris from hospital and municipal wastewater in Miami-Dade County, Florida DOI Creative Commons
Kristina M. Babler,

Mark Sharkey,

Sebastian Arenas

et al.

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 898, P. 165459 - 165459

Published: July 12, 2023

Language: Английский

Citations

24
Simultaneous detection and quantification of multiple pathogen targets in wastewater DOI Creative Commons
Gouthami Rao, Drew Capone, Kevin Zhu

et al.

PLOS Water, Journal Year: 2024, Volume and Issue: 3(2), P. e0000224 - e0000224

Published: Feb. 1, 2024

Wastewater-based epidemiology has emerged as a critical tool for public health surveillance, building on decades of environmental surveillance work pathogens such poliovirus. Work to date been limited monitoring single pathogen or small numbers in targeted studies; however, few studies consider simultaneous quantitative analysis wide variety pathogens, which could greatly increase the utility wastewater surveillance. We developed novel multi-pathogen approach (35 targets including bacteria, viruses, protozoa, and helminths) using TaqMan Array Cards (TAC) applied method concentrated samples collected at four treatment plants Atlanta, GA from February October 2020. From sewersheds serving approximately 2 million people, we detected range many expected find (e.g., enterotoxigenic E . coli Giardia 97% 29 stable concentrations) well unexpected Strongyloides stercoralis (a human threadworm rarely observed USA). Other notable detections included SARS-CoV-2, but also several that are not commonly like Acanthamoeba spp., Balantidium , Entamoeba histolytica astrovirus, norovirus, sapovirus. Our data suggest broad expanding scope enteric wastewaters, with potential application settings where quantification fecal waste streams can inform selection control measures limit infections.

Language: Английский

Citations

15
Critical Needs for Integrated Surveillance: Wastewater-Based and Clinical Epidemiology in Evolving Scenarios with Lessons Learned from SARS-CoV-2 DOI Creative Commons
Annalaura Carducci, Ileana Federigi,

Giulia Lauretani

et al.

Food and Environmental Virology, Journal Year: 2024, Volume and Issue: 16(1), P. 38 - 49

Published: Jan. 2, 2024

Abstract During the COVID-19 pandemic, wastewater-based epidemiology (WBE) and clinical surveillance have been used as tools for analyzing circulation of SARS-CoV-2 in community, but both approaches can be strongly influenced by some sources variability. From challenging perspective integrating environmental data, we performed a correlation analysis between concentrations raw sewage incident cases areas served medium-size wastewater treatment plants (WWTPs) from 2021 to 2023. To this aim, datasets were adjusted several variability: WBE data factors including analytical protocol, flow, population size, while adjustments considered demographic composition population. Then, addressed impact on differences among sewerage networks variations frequency type swab tests due changes political regulatory scenarios. Wastewater significantly correlated when restrictive containment measures limited movements effect ( ρ = 0.50) confirmed exclusively through molecular testing 0.49). Moreover, positive (although weak) arose WWTPs located densely populated 0.37) with shorter lengths 0.28). This study provides methodological interpreting which could also useful other infections. Data evaluation possible bias need carefully integrated

Language: Английский

Citations

8
Evaluation of plasmid pBI143 for its optimal concentration methods, seasonal impact, and potential as a normalization parameter in wastewater-based epidemiology DOI Creative Commons
Bikash Malla, Sadhana Shrestha, Niva Sthapit

et al.

The Science of The Total Environment, Journal Year: 2025, Volume and Issue: 965, P. 178661 - 178661

Published: Feb. 1, 2025

Language: Английский

Citations

1