Molecular mechanisms and physiological importance of circadian rhythms

Nature Reviews Molecular Cell Biology, Journal Year: 2019, Volume and Issue: 21(2), P. 67 - 84

Published: Nov. 25, 2019

Language: Английский

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A Single-Cell Transcriptome Atlas of the Aging Drosophila Brain

Cell, Journal Year: 2018, Volume and Issue: 174(4), P. 982 - 998.e20

Published: June 18, 2018

The diversity of cell types and regulatory states in the brain, how these change during aging, remains largely unknown. We present a single-cell transcriptome atlas entire adult Drosophila melanogaster brain sampled across its lifespan. Cell clustering identified 87 initial clusters that are further subclustered validated by targeted cell-sorting. Our data show high granularity identify wide range types. Gene network analyses using SCENIC revealed heterogeneity linked to energy consumption. During RNA content declines exponentially without affecting neuronal identity old brains. This covers nearly all cells normal provides tools study cellular alongside other mammalian datasets our unique analysis platform: SCope (http://scope.aertslab.org). These results, together with SCope, allow comprehensive exploration transcriptional an aging brain.

Language: Английский

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Circadian Rhythms and Sleep in Drosophila melanogaster

Genetics, Journal Year: 2017, Volume and Issue: 205(4), P. 1373 - 1397

Published: March 30, 2017

The advantages of the model organism Drosophila melanogaster, including low genetic redundancy, functional simplicity, and ability to conduct large-scale screens, have been essential for understanding molecular nature circadian (∼24 hr) rhythms, continue be valuable in discovering novel regulators rhythms sleep. In this review, we discuss current these interrelated biological processes wider implications research. Clock genes period timeless were first discovered screens developed 1970s. Feedback on their own transcription forms core clock, accurately timed expression, localization, post-transcriptional modification, function is thought critical maintaining cycle. Regulators, several phosphatases kinases, act different steps feedback loop ensure strong rhythms. Approximately 150 neurons fly brain that contain components clock together translate intracellular cycling into rhythmic behavior. We how groups serve functions allowing clocks entrain environmental cues, driving behavioral outputs at times day, flexible responses conditions. neuropeptide PDF provides an important signal synchronize neurons, although details accomplishes are still being explored. Secreted signals from also influence other tissues. SLEEP is, part, regulated by which ensures appropriate timing sleep, but amount quality sleep determined mechanisms a homeostatic balance between wake. Flies useful identifying large set genes, molecules, neuroanatomic loci regulating amount. Conserved aspects regulation flies mammals include wake-promoting roles catecholamine neurotransmitters involvement hypothalamus-like regions, regions implicated less clear parallels. Sleep subject factors such as food availability, stress, social environment. beginning understand identified molecules interact with each other, environment, regulate researchers can take advantage increasing mechanistic behaviors, learning memory, courtship, aggression, loss impacts behaviors. thus remain tool both discovery deep

Language: Английский

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Sleep Drive Is Encoded by Neural Plastic Changes in a Dedicated Circuit

Cell, Journal Year: 2016, Volume and Issue: 165(6), P. 1347 - 1360

Published: May 21, 2016

Language: Английский

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Recent advances in neuropeptide signaling in Drosophila, from genes to physiology and behavior

Progress in Neurobiology, Journal Year: 2019, Volume and Issue: 179, P. 101607 - 101607

Published: March 22, 2019

Language: Английский

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Circadian neuron feedback controls the Drosophila sleep–activity profile

Nature, Journal Year: 2016, Volume and Issue: 536(7616), P. 292 - 297

Published: Aug. 1, 2016

Language: Английский

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A Conserved Bicycle Model for Circadian Clock Control of Membrane Excitability

Cell, Journal Year: 2015, Volume and Issue: 162(4), P. 836 - 848

Published: Aug. 1, 2015

Language: Английский

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A Circadian Output Circuit Controls Sleep-Wake Arousal in Drosophila

Neuron, Journal Year: 2018, Volume and Issue: 100(3), P. 624 - 635.e4

Published: Sept. 27, 2018

Language: Английский

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The Regulation of Drosophila Sleep

Current Biology, Journal Year: 2021, Volume and Issue: 31(1), P. R38 - R49

Published: Jan. 1, 2021

Language: Английский

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A transcriptomic taxonomy of Drosophila circadian neurons around the clock

eLife, Journal Year: 2021, Volume and Issue: 10

Published: Jan. 13, 2021

Many different functions are regulated by circadian rhythms, including those orchestrated discrete clock neurons within animal brains. To comprehensively characterize and assign cell identity to the 75 pairs of Drosophila neurons, we optimized a single-cell RNA sequencing method assayed neuron gene expression at times day. The data identify least 17 categories with striking spatial regulation expression. Transcription factor is prominent likely contributes robust oscillation many transcripts, that encode cell-surface proteins previously shown be important for recognition synapse formation during development. other clock-regulated genes also constitute an resource future mechanistic functional studies between and/or temporal signaling circuits elsewhere in fly brain.

Language: Английский

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