Aedes aegypti Mosquitoes Detect Acidic Volatiles Found in Human Odor Using the IR8a Pathway

Current Biology, Journal Year: 2019, Volume and Issue: 29(8), P. 1253 - 1262.e7

Published: March 28, 2019

Language: Английский

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Cellular and Neural Responses to Sour Stimuli Require the Proton Channel Otop1

Current Biology, Journal Year: 2019, Volume and Issue: 29(21), P. 3647 - 3656.e5

Published: Sept. 19, 2019

The sense of taste allows animals to sample chemicals in the environment prior ingestion. Of five basic tastes, sour, acids, had remained among most mysterious. Acids are detected by type III receptor cells (TRCs), located buds across tongue and palate epithelium. first step sour transduction is believed be entry protons into cell cytosol, which leads cytosolic acidification generation action potentials. proton-selective ion channel Otop1 expressed TRCs a candidate receptor. Here, we tested contribution gustatory nerve responses acids mice was genetically inactivated (Otop1-KO mice). We show that required for inward proton current from different parts otherwise molecularly heterogeneous. next Otop1-KO mice, intracellular pH does not track with extracellular moderately acidic stimuli do elicit trains potentials, as they wild-type mice. Moreover, were severely selectively attenuated stimuli, including citric acid HCl. These results establish plays critical role detection mouse system, evidence it bona fide

Language: Английский

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Chemoreceptor co-expression in Drosophila melanogaster olfactory neurons

eLife, Journal Year: 2022, Volume and Issue: 11

Published: April 20, 2022

olfactory neurons have long been thought to express only one chemosensory receptor gene family. There are two main families in

Language: Английский

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Cross-modal sensory compensation increases mosquito attraction to humans

Science Advances, Journal Year: 2025, Volume and Issue: 11(1)

Published: Jan. 1, 2025

Sensory compensation occurs when loss of one sense leads to enhanced perception by another sense. We have identified a previously undescribed mechanism sensory in female Aedes aegypti mosquitoes. Odorant receptor co-receptor ( Orco ) mutants show attraction human skin temperature and increased heat-evoked neuronal activity foreleg neurons. Ir140 , foreleg-enriched member the ionotropic (IR) superfamily receptors, is up-regulated mutant legs. double do not heat seeking seen single mutants, suggesting that up-regulation key underlying mutants. Because expression sparse legs, this requires an indirect, long-range mechanism. Our findings highlight how mosquitoes, despite suffering olfactory loss, maintain overall effectiveness their host-seeking behavior up-regulating temperature, further enhancing status as most dangerous predator humans.

Language: Английский

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An expression atlas of variant ionotropic glutamate receptors identifies a molecular basis of carbonation sensing

Nature Communications, Journal Year: 2018, Volume and Issue: 9(1)

Published: Oct. 8, 2018

Abstract Through analysis of the Drosophila ionotropic receptors (IRs), a family variant glutamate receptors, we reveal that most IRs are expressed in peripheral neuron populations diverse gustatory organs larvae and adults. We characterise IR56d, which defines two anatomically-distinct classes proboscis: one responds to carbonated solutions fatty acids while other represents subset sugar- acid-sensing cells. Mutational indicates together with broadly-expressed co-receptors IR25a IR76b, is essential for physiological responses carbonation acids, but not sugars. further demonstrate both promote IR56d-dependent attraction flies, through different behavioural outputs. Our work provides toolkit investigating taste functions IRs, these required sensing, illustrates how system uses combinatorial expression sensory molecules distinct neurons coordinate behaviour.

Language: Английский

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A complex peripheral code for salt taste in Drosophila

eLife, Journal Year: 2018, Volume and Issue: 7

Published: Oct. 11, 2018

Each taste modality is generally encoded by a single, molecularly defined, population of sensory cells. However, salt stimulates multiple pathways in mammals and insects, suggesting more complex code for taste. Here, we examine coding Drosophila. After creating comprehensive molecular map comprised five discrete neuron classes across the fly labellum, find that four are activated salt: two exhibiting characteristics ‘low salt’ cells, ‘high classes. Behaviorally, low attraction depends primarily on ‘sweet’ neurons, with additional input from neurons expressing ionotropic receptor IR94e. High avoidance mediated ‘bitter’ glutamatergic Ppk23. Interestingly, impact these prior consumption. These results support model flies combinatorially integrates inputs cell types to afford robust flexible behaviors.

Language: Английский

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A chromosome-level genome assembly of Cydia pomonella provides insights into chemical ecology and insecticide resistance

Nature Communications, Journal Year: 2019, Volume and Issue: 10(1)

Published: Sept. 17, 2019

Abstract The codling moth Cydia pomonella , a major invasive pest of pome fruit, has spread around the globe in last half century. We generated chromosome-level scaffold assembly including Z chromosome and portion W chromosome. This reveals duplication an olfactory receptor gene ( OR3 ), which we demonstrate enhances ability C. to exploit kairomones pheromones locating both host plants mates. Genome-wide association studies contrasting insecticide-resistant susceptible strains identify hundreds single nucleotide polymorphisms (SNPs) potentially associated with insecticide resistance, three SNPs found promoter CYP6B2 . RNAi knockdown increases sensitivity two insecticides, deltamethrin azinphos methyl. high-quality genome informs genetic basis its invasiveness, suggesting distinctive capabilities adaptive potential that may explain worldwide expansion.

Language: Английский

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Molecular basis of fatty acid taste in Drosophila

eLife, Journal Year: 2017, Volume and Issue: 6

Published: Dec. 12, 2017

Behavioral studies have established that Drosophila appetitive taste responses towards fatty acids are mediated by sweet sensing Gustatory Receptor Neurons (GRNs). Here we show GRN activation requires the function of Ionotropic genes IR25a, IR76b and IR56d. The former two IR expressed in several neurons per sensillum, while IR56d expression is restricted to GRNs. Importantly, loss behavioral IR25a mutant flies can be completely rescued respective transgenes Interestingly, wild type hexanoic acid reach a plateau at ~1%, but decrease with higher concentration, property through IR25a/IR76b independent bitter With our previous report on sour taste, suggest IR-based receptors mediate different qualities cell-type specific subunits.

Language: Английский

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Calcium Taste Avoidance in Drosophila

Neuron, Journal Year: 2017, Volume and Issue: 97(1), P. 67 - 74.e4

Published: Dec. 21, 2017

Language: Английский

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Recent advances in the genetic basis of taste detection in Drosophila

Cellular and Molecular Life Sciences, Journal Year: 2019, Volume and Issue: 77(6), P. 1087 - 1101

Published: Oct. 9, 2019

Language: Английский

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