An expression atlas of variant ionotropic glutamate receptors identifies a molecular basis of carbonation sensing

Nature Communications, Journal Year: 2018, Volume and Issue: 9(1)

Published: Oct. 8, 2018

Abstract Through analysis of the Drosophila ionotropic receptors (IRs), a family variant glutamate receptors, we reveal that most IRs are expressed in peripheral neuron populations diverse gustatory organs larvae and adults. We characterise IR56d, which defines two anatomically-distinct classes proboscis: one responds to carbonated solutions fatty acids while other represents subset sugar- acid-sensing cells. Mutational indicates together with broadly-expressed co-receptors IR25a IR76b, is essential for physiological responses carbonation acids, but not sugars. further demonstrate both promote IR56d-dependent attraction flies, through different behavioural outputs. Our work provides toolkit investigating taste functions IRs, these required sensing, illustrates how system uses combinatorial expression sensory molecules distinct neurons coordinate behaviour.

Language: Английский

---

A complex peripheral code for salt taste in Drosophila

eLife, Journal Year: 2018, Volume and Issue: 7

Published: Oct. 11, 2018

Each taste modality is generally encoded by a single, molecularly defined, population of sensory cells. However, salt stimulates multiple pathways in mammals and insects, suggesting more complex code for taste. Here, we examine coding Drosophila. After creating comprehensive molecular map comprised five discrete neuron classes across the fly labellum, find that four are activated salt: two exhibiting characteristics ‘low salt’ cells, ‘high classes. Behaviorally, low attraction depends primarily on ‘sweet’ neurons, with additional input from neurons expressing ionotropic receptor IR94e. High avoidance mediated ‘bitter’ glutamatergic Ppk23. Interestingly, impact these prior consumption. These results support model flies combinatorially integrates inputs cell types to afford robust flexible behaviors.

Language: Английский

---

Recent advances in the genetic basis of taste detection in Drosophila

Cellular and Molecular Life Sciences, Journal Year: 2019, Volume and Issue: 77(6), P. 1087 - 1101

Published: Oct. 9, 2019

Language: Английский

---

Drosophilasensory receptors—a set of molecular Swiss Army Knives

Genetics, Journal Year: 2021, Volume and Issue: 217(1), P. 1 - 34

Published: Jan. 1, 2021

Genetic approaches in the fruit fly, Drosophila melanogaster, have led to a major triumph field of sensory biology-the discovery multiple large families receptors and channels. Some these families, such as transient receptor potential channels, are conserved from animals ranging worms humans, while others, "gustatory receptors," "olfactory "ionotropic restricted invertebrates. Prior identification flies, it was widely assumed that proteins function just one modality vision, smell, taste, hearing, somatosensation, which includes thermosensation, light, noxious mechanical touch. By employing vast combination genetic, behavioral, electrophysiological, other concept emerge is many multitaskers. The earliest example this idea individual channels senses. It now clear multitasking exhibited by including gustatory receptors, ionotropic epithelial Na+ (also referred Pickpockets), even opsins, were formerly thought exclusively light sensors. characterizations neurons express them also reveal mechanisms through flies can accurately differentiate between different stimuli when they activate same receptor, well adaptation, amplification, integration. insights gleaned studies been highly influential directing investigations animal models.

Language: Английский

---

Mechanism of Acetic Acid Gustatory Repulsion in Drosophila

Cell Reports, Journal Year: 2019, Volume and Issue: 26(6), P. 1432 - 1442.e4

Published: Feb. 1, 2019

The decision to consume or reject a food based on the degree of acidity is critical for animal survival. However, gustatory receptors that detect sour compounds and influence feeding behavior have been elusive. Here, using fly, Drosophila melanogaster, we reveal member ionotropic receptor family, IR7a, essential rejecting foods laced with high levels acetic acid. IR7a dispensable repulsion other acidic compounds, indicating sensation acids occurs through repertoire rather than single receptor. fly’s main taste organ, labellum, decorated bristles house dendrites neurons (GRNs). expressed in subset bitter GRNs dedicated taste. Our findings indicate flies receptors, enabling them discriminate basis acid composition just pH.

Language: Английский

---

Molecular mechanisms of olfactory detection in insects: beyond receptors

Open Biology, Journal Year: 2020, Volume and Issue: 10(10)

Published: Oct. 1, 2020

Insects thrive in diverse ecological niches large part because of their highly sophisticated olfactory systems. Over the last two decades, a major focus study insect olfaction has been on role receptors mediating neuronal responses to environmental chemicals. In vivo , these operate specialized structures, called sensilla, which comprise neurons and non-neuronal support cells, extracellular lymph fluid precisely shaped cuticle. While sensilla are inherent odour sensing insects, we only just beginning understand construction function. Here, review recent work that illuminates how odour-evoked activity is impacted by sensillar morphology, biochemistry, accessory signalling molecules physiological crosstalk between cells. These advances reveal multi-layered molecular cellular mechanisms determine selectivity, sensitivity dynamic modulation insects.

Language: Английский

---

The Structure and Function of Ionotropic Receptors in Drosophila

Frontiers in Molecular Neuroscience, Journal Year: 2021, Volume and Issue: 13

Published: Feb. 1, 2021

Ionotropic receptors (IRs) are a highly divergent subfamily of ionotropic glutamate (iGluR) and conserved across Protostomia, major branch the animal kingdom that encompasses both Ecdysozoa Lophothrochozoa. They broadly expressed in peripheral sensory systems, concentrated dendrites, function chemosensation, thermosensation, hygrosensation. As iGluRs, four IR subunits form functional ion channel to detect environmental stimuli. Most comprise individual stimulus-specific tuning one or two coreceptors. This review summarizes discoveries structure complexes expression each IR, as well discusses future direction for studies.

Language: Английский

---

Molecular and neuronal mechanisms for amino acid taste perception in the Drosophila labellum

Current Biology, Journal Year: 2022, Volume and Issue: 32(6), P. 1376 - 1386.e4

Published: Feb. 16, 2022

Language: Английский

---

A molecular mechanism for high salt taste in Drosophila

Current Biology, Journal Year: 2022, Volume and Issue: 32(14), P. 3070 - 3081.e5

Published: June 29, 2022

Language: Английский

---

Molecular sensors in the taste system of Drosophila

Genes & Genomics, Journal Year: 2023, Volume and Issue: 45(6), P. 693 - 707

Published: Feb. 24, 2023

Language: Английский

---