The ISME Journal,
Journal Year:
2024,
Volume and Issue:
18(1)
Published: Jan. 1, 2024
Microorganisms
colonizing
plant
roots
co-exist
in
complex,
spatially
structured
multispecies
biofilm
communities.
However,
little
is
known
about
microbial
interactions
and
the
underlying
spatial
organization
within
communities
established
on
roots.
Here,
a
well-established
four-species
model
(Stenotrophomonas
rhizophila,
Paenibacillus
amylolyticus,
Microbacterium
oxydans,
Xanthomonas
retroflexus,
termed
as
SPMX)
was
applied
to
Arabidopsis
study
impact
of
growth
community
dynamics
SPMX
co-culture
notably
promoted
root
development
biomass.
Co-cultured
increased
colonization
formed
biofilms,
structurally
different
from
those
by
monocultures.
By
combining
16S
rRNA
gene
amplicon
sequencing
fluorescence
situ
hybridization
with
confocal
laser
scanning
microscopy,
we
found
that
composition
significantly
changed
over
time.
Monoculture
P.
amylolyticus
colonized
poorly,
but
its
population
were
highly
enhanced
when
residing
biofilm.
Exclusion
reduced
overall
production
three
species,
resulting
loss
growth-promoting
effects.
Combined
analysis,
this
led
identification
keystone
species.
Our
findings
highlight
weak
colonizers
may
benefit
mutualistic
complex
hereby
become
important
species
impacting
function.
This
work
expands
knowledge
uncovering
interspecific
roots,
beneficial
for
harnessing
mutualism
promoting
growth.
Annual Review of Ecology Evolution and Systematics,
Journal Year:
2019,
Volume and Issue:
50(1), P. 145 - 168
Published: July 24, 2019
Microorganisms
drive
several
processes
needed
for
robust
plant
growth
and
health.
Harnessing
microbial
functions
is
thus
key
to
productive
sustainable
food
production.
Molecular
methods
have
led
a
greater
understanding
of
the
soil
microbiome
composition.
However,
translating
species
or
gene
composition
into
functionality
remains
challenge.
Community
ecology
concepts
such
as
biodiversity–ecosystem
functioning
framework
may
help
predict
assembly
function
plant-associated
microbiomes.
Higher
diversity
can
increase
number
resilience
plant-beneficial
that
be
coexpressed
unlock
expression
traits
are
hard
obtain
from
any
in
isolation.
We
combine
well-established
community
with
molecular
microbiology
workable
enable
us
enhance
promote
global
change
context.
Nature Microbiology,
Journal Year:
2021,
Volume and Issue:
6(2), P. 151 - 156
Published: Jan. 4, 2021
Abstract
Biofilms
are
microbial
communities
that
represent
a
highly
abundant
form
of
life
on
Earth.
Inside
biofilms,
phenotypic
and
genotypic
variations
occur
in
three-dimensional
space
time;
microscopy
quantitative
image
analysis
therefore
crucial
for
elucidating
their
functions.
Here,
we
present
BiofilmQ—a
comprehensive
cytometry
software
tool
the
automated
high-throughput
quantification,
visualization
numerous
biofilm-internal
whole-biofilm
properties
time.
Molecular Plant-Microbe Interactions,
Journal Year:
2020,
Volume and Issue:
34(1), P. 15 - 25
Published: Sept. 28, 2020
Bacillus
subtilis
is
one
of
the
most
widely
studied
plant
growth–promoting
rhizobacteria.
It
able
to
promote
growth
as
well
control
pathogens
through
diverse
mechanisms,
including
improvement
nutrient
availability
and
alteration
phytohormone
homeostasis
production
antimicrobials
triggering
induced
systemic
resistance,
respectively.
Even
though
its
benefits
for
crop
have
been
recognized
extensively
under
laboratory
conditions,
success
application
in
fields
varies
immensely.
accepted
that
agricultural
B.
often
fails
because
bacteria
are
not
persist
rhizosphere.
Bacterial
colonization
roots
a
crucial
step
interaction
between
microbe
seems,
therefore,
be
great
importance
promotion
biocontrol
effects.
A
successful
root
depends
thereby
on
both
bacterial
traits,
motility
biofilm
formation,
signal
interplay
with
plant.
This
review
addresses
current
knowledge
about
plant-microbial
interactions
species,
various
mechanisms
supporting
necessity
establishment
relationship.
The
author(s)
dedicated
work
public
domain
Creative
Commons
CC0
"No
Rights
Reserved"
license
by
waiving
all
his
or
her
rights
worldwide
copyright
law,
related
neighboring
rights,
extent
allowed
law.
npj Biofilms and Microbiomes,
Journal Year:
2021,
Volume and Issue:
7(1)
Published: Nov. 10, 2021
Abstract
Biofilms
are
organised
heterogeneous
assemblages
of
microbial
cells
that
encased
within
a
self-produced
matrix.
Current
estimates
suggest
up
to
80%
bacterial
and
archaeal
reside
in
biofilms.
Since
biofilms
the
main
mode
life,
understanding
their
biology
functions
is
critical,
especially
as
controlling
biofilm
growth
essential
industrial,
infrastructure
medical
contexts.
Here
we
discuss
both
collections
individual
cells,
multicellular
biological
individuals,
introduce
concept
unique
incubators
diversity
for
world.
The
second
messenger
signaling
molecule
cyclic
diguanylate
monophosphate
(c-di-GMP)
drives
the
transition
between
planktonic
and
biofilm
growth
in
many
bacterial
species.
Pseudomonas
aeruginosa
has
two
surface
sensing
systems
that
produce
c-di-GMP
response
to
adherence.
Current
thinking
field
is
once
cells
attach
a
surface,
they
uniformly
respond
by
producing
c-di-GMP.
Here,
we
describe
how
Wsp
system
generates
heterogeneity
sensing,
resulting
physiologically
distinct
subpopulations
of
cells.
One
subpopulation
elevated
produces
matrix,
serving
as
founders
initial
microcolonies.
other
low
engages
motility,
allowing
for
exploration
surface.
We
also
show
this
strongly
correlates
behavior
descendent
Together,
our
results
suggest
after
attachment,
P.
division
labor
persists
across
generations,
accelerating
early
formation
exploration.
Molecular Microbiology,
Journal Year:
2018,
Volume and Issue:
110(6), P. 863 - 878
Published: Sept. 16, 2018
Summary
Here,
we
review
the
multiple
mechanisms
that
Gram‐positive
bacterium
Bacillus
subtilis
uses
to
allow
it
communicate
between
cells
and
establish
community
structures.
The
modes
of
action
are
used
highly
varied
include
routes
sense
pheromone
levels
during
quorum
sensing
control
gene
regulation,
intimate
coupling
via
nanotubes
share
cytoplasmic
contents,
long‐range
electrical
signalling
couple
metabolic
processes
both
within
biofilms.
We
explore
ability
B.
detect
‘kin’
(and
‘cheater
cells’)
by
looking
at
potentially
ensure
beneficial
sharing
(or
limit
exploitation)
extracellular
‘public
goods’.
Finally,
reflecting
on
array
methods
a
single
has
its
disposal
maximal
benefit
for
progeny,
highlight
large
future
challenge
will
be
integrating
how
these
systems
interact
in
mixed‐species
communities.
