A genome-wide association study implicates the olfactory system in Drosophila melanogaster diapause-associated lifespan extension and fecundity DOI Open Access
Sreesankar Easwaran, Denise J. Montell

Published: June 12, 2024

The effects of environmental stress on animal life are gaining importance with climate change. Diapause is a dormancy program that occurs in response to an adverse environment, followed by resumption development and reproduction upon the return favorable conditions. complex trait, so we leveraged Drosophila genetic reference panel (DGRP) lines conducted Genome-Wide Association Study (GWAS) characterize basis diapause. We assessed post-diapause non-diapause fecundity across all DGRP lines. GWAS revealed 546 variants, encompassing single nucleotide polymorphisms, insertions deletions associated fecundity. identified 291 candidate diapause-associated genes, 40 which had previously been 89 candidates were more than one SNP. Gene network analysis indicated genes primarily linked neuronal reproductive system development. Similarly, comparison results from other fly greatest overlap olfactory-behavior-associated fecundity-and-lifespan-associated genes. An RNAi screen top two Dip- γ Scribbler, be required during recovery for complemented test neurons successful found although amputation antenna little no effect lifespan, it reduced diapause lifespan postdiapause further show olfactory receptor temperature-sensing Our provide insights into molecular, cellular, adult Drosophila.

Language: Английский

The Neuronal Circuit of the Dorsal Circadian Clock Neurons in Drosophila melanogaster DOI Creative Commons
Nils Reinhard, Frank K. Schubert, Enrico Bertolini

et al.

Frontiers in Physiology, Journal Year: 2022, Volume and Issue: 13

Published: April 29, 2022

Drosophila's dorsal clock neurons (DNs) consist of four clusters (DN1as, DN1ps, DN2s, and DN3s) that largely differ in size. While the DN1as DN2s encompass only two neurons, DN1ps ∼15 DN3s comprise ∼40 per brain hemisphere. In comparison to well-characterized lateral (LNs), neuroanatomy function DNs are still not clear. Over past decade, numerous studies have addressed their role fly's circadian system, leading several sometimes divergent results. Nonetheless, these agreed important fine-tune activity under light temperature cycles play essential roles linking output from LNs downstream control sleep metabolism. Here, we used Flybow specific split-GAL4 lines, trans-Tango, recently published fly connectome (called hemibrain) describe morphology greater detail, including synaptic connections other non-clock neurons. We show some DN groups heterogenous. certain strongly connected with LNs, others mainly signal circuits clock. Among latter mushroom body central complex tubercle bulb neurosecretory cells pars intercerebralis, unidentified partners. This heterogeneity may explain conflicting results previously found about functionality. Most importantly, identify putative novel communication centers network: one fiber bundle superior protocerebrum running toward anterior optic hub posterior protocerebrum. Both invaded by might an instrumental network.

Language: Английский

Citations

39
Seasonal cues act through the circadian clock and pigment-dispersing factor to control EYES ABSENT and downstream physiological changes DOI Creative Commons
Sergio Hidalgo, Maribel Anguiano, Christine A. Tabuloc

et al.

Current Biology, Journal Year: 2023, Volume and Issue: 33(4), P. 675 - 687.e5

Published: Jan. 27, 2023

Language: Английский

Citations

39
The molecular mechanisms of diapause and diapause-like reversible arrest DOI Creative Commons
Sreesankar Easwaran, Denise J. Montell

Biochemical Society Transactions, Journal Year: 2023, Volume and Issue: 51(5), P. 1847 - 1856

Published: Oct. 6, 2023

Diapause is a protective mechanism that many organisms deploy to overcome environmental adversities. extends lifespan and fertility enhance the reproductive success survival of species. Although diapause states have been known employed for commercial purposes, example in silk industry, detailed molecular cell biological studies are an exciting frontier. Understanding diapause-like mechanisms will shed light on pathways steer through adverse conditions. One hope understanding support might be leveraged extend and/or health span humans as well species threatened by climate change. In addition, recent findings suggest cancer cells persist after treatment mimic states, implying these programs may facilitate from chemotherapy cause relapse. Here, we review underlying variety organisms, discuss supporting tumor persister cells.

Language: Английский

Citations

29
Female reproductive dormancy in Drosophila is regulated by DH31-producing neurons projecting into the corpus allatum DOI Creative Commons
Yoshitomo Kurogi, Eisuke Imura, Yosuke Mizuno

et al.

Development, Journal Year: 2023, Volume and Issue: 150(10)

Published: May 15, 2023

ABSTRACT Female insects can enter reproductive diapause, a state of suspended egg development, to conserve energy under adverse environments. In many insects, including the fruit fly, Drosophila melanogaster, also frequently called dormancy, is induced low-temperature and short-day conditions by downregulation juvenile hormone (JH) biosynthesis in corpus allatum (CA). this study, we demonstrate that neuropeptide Diuretic 31 (DH31) produced brain neurons project into CA plays an essential role regulating dormancy suppressing JH adult D. melanogaster. The expresses gene encoding DH31 receptor, which required for DH31-triggered elevation intracellular cAMP CA. Knocking down Dh31 these CA-projecting or receptor suppresses decrease titer, normally observed dormancy-inducing conditions, leading abnormal yolk accumulation ovaries. Our findings provide first molecular genetic evidence demonstrating peptidergic play biosynthesis.

Language: Английский

Citations

24
Dynamic encoding of temperature in the central circadian circuit coordinates physiological activities DOI Creative Commons
Hailiang Li, Zhiyi Li,

Xin Yuan

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: April 2, 2024

Abstract The circadian clock regulates animal physiological activities. How temperature reorganizes circadian-dependent activities remains elusive. Here, using in-vivo two-photon imaging with the control device, we investigated response of Drosophila central circuit to variation and identified that DN1as serves as most sensitive temperature-sensing neurons. gate DN1a’s diurnal response. Trans-synaptic tracing, connectome analysis, functional data reveal bidirectionally targets two neuronal subsets: activity-related E cells sleep-promoting DN3s. Specifically, behavioral demonstrate DN1a-E cell modulates evening locomotion peak in cold temperature, while DN1a-DN3 controls warm temperature-induced nocturnal sleep reduction. Our findings systematically comprehensively illustrate how dynamically integrates light signals effectively coordinate wakefulness at different times day, shedding on conserved neural mechanisms underlying temperature-regulated physiology animals.

Language: Английский

Citations

10
Neuropeptidergic regulation of insect diapause by the circadian clock DOI Creative Commons
Charlotte Helfrich‐Förster

Current Opinion in Insect Science, Journal Year: 2024, Volume and Issue: 63, P. 101198 - 101198

Published: April 6, 2024

Diapause is an endocrine-mediated strategy used by insects to survive seasons of adverse environmental conditions. Insects living in temperate zones are regularly exposed such conditions the form winter. To winter, they must prepare for it long before arrives. A reliable indicator impending winter shortening day length. measure length, need their circadian clock as internal time reference. In this article, I provide overview current state knowledge on neuropeptides that link diapause inducing hormonal brain centers.

Language: Английский

Citations

8
Contribution of neurons that express fruitless and Clock transcription factors to behavioral rhythms and courtship DOI Creative Commons

Anthony Deluca,

Brooke Bascom,

Daniela A Key Planas

et al.

iScience, Journal Year: 2025, Volume and Issue: 28(3), P. 112037 - 112037

Published: Feb. 16, 2025

Animals need to integrate information across neuronal networks that direct reproductive behaviors and circadian rhythms. The Drosophila master regulatory transcription factors courtship rhythms are co-expressed. We find sex differences in the number of these fruitless (fru) Clock (Clk)-expressing neurons (fru ∩ Clk neurons) regulated by male-specific Fru. assign fru electron microscopy connectome subtypes clock neurons. discover fru-expressing post-synaptic targets Clk-expressing When activated or silenced, we observe a shortening period length. Activation also changes rate behavior is performed. examine male over 24 h activities peak at lights-on. These results reveal how subserve two processes can impact behavioral outcomes sex-specific manner.

Language: Английский

Citations

1
A thermometer circuit for hot temperature adjusts Drosophila behavior to persistent heat DOI Creative Commons
Michael H. Alpert,

Hamin Gil,

Alessia Para

et al.

Current Biology, Journal Year: 2022, Volume and Issue: 32(18), P. 4079 - 4087.e4

Published: Aug. 17, 2022

Language: Английский

Citations

22
Altered circadian rhythm, sleep, and rhodopsin 7 –dependent shade preference during diapause in Drosophila melanogaster DOI Creative Commons

Geoff T. Meyerhof,

Sreesankar Easwaran,

Angela E. Bontempo

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(27)

Published: June 25, 2024

To survive adverse environments, many animals enter a dormant state such as hibernation, dauer, or diapause. Various Drosophila species undergo adult reproductive diapause in response to cool temperatures and/or short day-length. While flies are less active during diapause, it is unclear how environmental conditions affect circadian rhythms and sleep. Here we show that diapause-inducing temperatures, melanogaster exhibit altered activity profiles, including severely reduced morning an advanced evening peak. Consequently, the have single peak at time similar when nondiapausing take siesta. Temperatures ≤15 °C, rather than photoperiod, primarily drive this behavior. At rapidly deep-sleep lacks sleep cycles of higher require high levels stimulation for arousal. Furthermore, 25 prefer siesta shade, preference virtually eliminated 10 °C. Resting shade driven by aversion blue light sensed Rhodopsin 7 outside eyes. Flies °C neuronal markers elevated pressure, increased expression Bruchpilot Ca 2+ R5 ellipsoid body neurons. Therefore, pressure might overcome aversion. Thus, same cause arrest, preserve germline stem cells, extend lifespan, D. prone deep dramatically altered, yet rhythmic, daily patterns.

Language: Английский

Citations

4
What Drosophila can tell us about state-dependent peptidergic signaling in insects DOI Creative Commons
Dick R. Nässel

Insect Biochemistry and Molecular Biology, Journal Year: 2025, Volume and Issue: unknown, P. 104275 - 104275

Published: Feb. 1, 2025

Language: Английский

Citations

0