Virus-specific Th17 Cells Are Induced by Human Cytomegalovirus after Renal Transplantation DOI
Ravi Dhital, Kaitlyn Flint, Irina Kaptsan

et al.

The Journal of Immunology, Journal Year: 2024, Volume and Issue: 213(11), P. 1703 - 1712

Published: Oct. 18, 2024

Abstract CMV infection and Th17 cells are independently associated with increased risk for late allograft loss after renal transplantation. Although CMV-specific detectable in animal models nontransplant clinical populations, evidence linking transplantation remains unclear. This prospective observational study evaluated a cohort of transplant recipients during 12 mo posttransplant to assess the presence peripheral blood their relationship pretransplant serostatus DNAemia. were identified among donor (D)+ and/or recipient (R)+ expanded both primary (D+/R−) reactivated (D+/R+, D−/R+) A subset coexpressed IFN-γ, indicating Th1/17 phenotype. These expressed CCR6, CCR5, activation terminal differentiation markers (CD95, OX40, HLA-DR, CD57), central/effector memory activating/inhibitory receptors (CD57, 4-1BB, CD160, CTLA-4, PD-1) at higher frequencies than cells. In contrast, staphylococcal enterotoxin B–induced did not expand DNAemia, differ between groups over time, predominantly TNF-α, had lower expression activating inhibitory pp65-specific data show that episodes DNAemia recipients, these virus-specific have distinct phenotypes from global circulating Th(1)/17 results suggest potential proinflammatory pathway by which CMV-induced may contribute injury, increasing loss.

Language: Английский

Recent advances in the development and clinical application of miRNAs in infectious diseases DOI Creative Commons
Sara Nunes,

Rana Bastos,

Ana Clara Cardoso Marinho

et al.

Non-coding RNA Research, Journal Year: 2024, Volume and Issue: 10, P. 41 - 54

Published: Sept. 2, 2024

In the search for new biomarkers and therapeutic targets infectious diseases, several molecules have been investigated. Small RNAs, known as microRNAs (miRs), are important regulators of gene expression, emerged promising candidates these purposes. MiRs a class small, endogenous non-coding RNAs that play critical roles in human including host-pathogen interaction mechanisms. Recently, miRs signatures reported different opening perspectives molecular diagnosis therapy. MiR profiles can discriminate between healthy individuals patients, well distinguish disease stages. Furthermore, possibility assessing biological fluids, such serum whole blood, renders feasible development non-invasive diagnostic prognostic tools. this manuscript, we will comprehensively describe diseases explore how they contribute to advance existing Additionally, discuss miR analysis platforms understand obstacles advances approach propose their potential clinical applications contributions public health.

Language: Английский

Citations

11
Critical Role of Cellular microRNAs in Virus Infection: Decades of Progress DOI Creative Commons
Yaqi Han, Guoqing Zhang,

Xinru Lv

et al.

Animals and zoonoses., Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

1
Suppression of miR-155 Attenuates Lung Cytokine Storm Induced by SARS-CoV-2 Infection DOI
Dharmendra Kumar Soni,

Juan Cabrera‐Luque,

Swagata Kar

et al.

Journal of Interferon & Cytokine Research, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 14, 2025

Coronavirus disease 2019 (COVID-19) is a deadly human viral with high rate of infection, morbidity, and mortality. Although vaccines antiviral treatments are available, hospitalizations remain steady, concerns about long-term consequences persist. Therefore, there great urgency to develop novel therapies. Here, we analyzed the role miR-155, one most powerful drivers host responses including immune inflammatory responses, in pathogenicity severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Endogenous microRNAs (miRNAs, miRs) key molecules preventing entry replication while building an cellular defense. Our study reveals that miR-155 expression elevated patients COVID-19. Using mouse model transgenic for angiotensin-converting enzyme receptor 2, evaluated potential anti-miR-155 therapy. Treating SARS-CoV-2-infected mice significantly reduced expression, improved survival, slightly increased body weight. Notably, these showed altered cytokines lungs. These findings suggest could be promising therapy mitigate cytokine storm long-lasting symptoms induced by SARS-CoV-2 improving public health outcomes enhancing global pandemic preparedness.

Language: Английский

Citations

1
SOCS-JAK-STAT inhibitors and SOCS mimetics as treatment options for autoimmune uveitis, psoriasis, lupus, and autoimmune encephalitis DOI Creative Commons
Rahul Pandey,

Marina Bakay,

Hákon Hákonarson

et al.

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: Oct. 26, 2023

Autoimmune diseases arise from atypical immune responses that attack self-tissue epitopes, and their development is intricately connected to the disruption of JAK-STAT signaling pathway, where SOCS proteins play crucial roles. Conditions such as autoimmune uveitis, psoriasis, lupus, encephalitis exhibit system dysfunctions associated with dysregulation. Emerging therapeutic strategies utilize inhibitors mimetics modulate alleviate manifestations. Although more research clinical studies are required assess effectiveness, safety profiles, potential for personalized approaches in conditions, show promise treatment options. This review explores action, future prospects JAK agents systemic lupus erythematosus, encephalitis. The findings underscore importance investigating these targeted therapies advance options individuals suffering diseases.

Language: Английский

Citations

18
The Potential Roles of Host Cell miRNAs in Fine-Tuning Bovine Coronavirus (BCoV) Molecular Pathogenesis, Tissue Tropism, and Immune Regulation DOI Creative Commons

Abid Ullah Shah,

Maged Gomaa Hemida

Microorganisms, Journal Year: 2024, Volume and Issue: 12(5), P. 897 - 897

Published: April 30, 2024

Bovine coronavirus (BCoV) infection causes significant economic loss to the dairy and beef industries worldwide. BCoV exhibits dual tropism, infecting respiratory enteric tracts of cattle. The isolates could also induce manifestations under certain circumstances. However, mechanism this tropism has not yet been studied well. MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression play a role in virus infection, mediating or modulating host immune regulatory genes through complex virus–host cell interactions. their remains unclear. This study aims identify bovine miRNAs crucial for regulating interaction, influencing tissue explore potential as biomarkers therapeutic agents against BCoV. We downloaded 18 full-length genomes (10 eight respiratory) from GenBank. applied several bioinformatic tools targeting various regions viral genome. used criteria differential between enteric/respiratory some critical biological markers infection. Using online tools, we searched miRNA target involved evasion, regulation. Our results show four (miR-2375, miR-193a-3p, miR-12059, miR-494) potentially spike protein at multiple sites. These suppressor pathways, which negatively impacts replication. Furthermore, found bta-(miR-2338, miR-6535, miR-2392, miR-12054) genome but signal transduction i.e., type I interferon (IFN) retinoic acid-inducible (RIG-I) pathways. Moreover, both miR-2338 miR-2392 transcriptional factors RORA, YY1, HLF, diagnostic Therefore, miR-2338, miR-12054 have fine-tune evasion enhance pathogenesis. indicate essential roles pathogenesis, Four bta-miR-193a-3p, bta-miR-12059, bta-miR-494) BCoV-S glycoprotein suppression pathways during candidates serve good genetic further studies urgently needed validate these identified context markers.

Language: Английский

Citations

5
Role of CXCL12/CXCR4 pathway and miRNA expression profiles on polymorphonuclear mobilization in COVID-19 patients DOI Creative Commons
Matthieu Daniel, Florent Bernardin,

Laëtitia Sennsfelder

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 10, 2025

Abstract Introduction: Polymorphonuclear neutrophils (PMN) are actively recruited during COVID-19 and yet dysfunctions associated with its prognosis. The PMN receptor CXCR4 ligand SDF-1/CXCL12 known to play a role in the recruitment of PMN. primary objective was evaluate modulation this pathway patients after treatment dexamethasone (DXM). Secondary objectives were miRNA expression profiles. Material Methods We conducted prospective study comparing admitted emergency department from December 2022 April 2023 for SARS-CoV-2 infection control population. studied surface receptor, circulating levels SDF-1 miR levels. Patients treated (DXM) sampled again at H48. Results Forty-four infected 20 controls analyzed. significantly increased decreased by DXM + percentages significantly. on admission risk mechanical ventilation. Levels 15b-5p, 146a-5p, 155-5p 30d-5p patients. miR-hsa-122 found mortality variation need Conclusions Our suggests possible involvement SDF-1/CXCR4 axis physiopathogenesis COVID-19.

Language: Английский

Citations

0
Altered expression of miRNA profile in peripheral blood mononuclear cells following the third dose of inactivated COVID-19 vaccine DOI Creative Commons

Guanguan Qiu,

Ruoyang Zhang,

Huifeng Qian

et al.

PeerJ, Journal Year: 2025, Volume and Issue: 13, P. e18856 - e18856

Published: Jan. 22, 2025

COVID-19 vaccination is the most effective strategy for preventing severe disease and death. Inactivated vaccines are accessible type of in developing countries. Several studies, including work from our group, have demonstrated that third dose (booster vaccination) inactivated vaccine induces robust humoral cellular immune responses. The present study aimed to examine miRNA expression profile participants who received a homologous CoronaVac vaccine. Samples peripheral blood mononuclear cells (PBMCs) were collected healthcare volunteers both before 1–2 weeks after booster dose. microarray analysis discovery cohort six identified 67 miRNAs with differential expression. Subsequently, related responses was examined validation 31 via qRT-PCR. Our results validated miR-25-5p, miR-34c-3p, miR-206 post-booster, significant correlation receptor binding domain (RBD)-specific antibody. Bioinformatic suggested may target multiple pathways involved regulation inflammation. Therefore, highlights PBMCs as promising biomarkers assessing response induced by

Language: Английский

Citations

0
Altered gene expression of miR-155 in peripheral blood mononuclear cells of Multiple sclerosis patients: Correlation with TH17 frequency, inflammatory cytokine profile and autoimmunity DOI
Shiva Alipour,

Pedram Amanallahi,

Behzad Baradaran

et al.

Multiple Sclerosis and Related Disorders, Journal Year: 2024, Volume and Issue: 89, P. 105764 - 105764

Published: July 7, 2024

Language: Английский

Citations

3
Immune modulatory microRNAs in tumors, their clinical relevance in diagnosis and therapy DOI Creative Commons
Christoforos Vaxevanis, Michael Bachmann, Barbara Seliger

et al.

Journal for ImmunoTherapy of Cancer, Journal Year: 2024, Volume and Issue: 12(8), P. e009774 - e009774

Published: Aug. 1, 2024

The importance of the immune system in regulating tumor growth by inducing cell-mediated cytotoxicity associated with patients' outcomes has been highlighted past years an increasing life expectancy patients cancer on treatment different immunotherapeutics. However, tumors often escape surveillance, which is accomplished mechanisms. Recent studies demonstrated essential role small non-coding RNAs, such as microRNAs (miRNAs), post-transcriptional control modulatory molecules. Multiple methods have used to identify miRNAs targeting genes involved escaping recognition including CTLA-4, PD-L1, HLA-G, components major histocompatibility class I antigen processing machinery (APM) well other response-relevant tumors. Due their function, these can be (1) diagnostic and prognostic biomarkers allowing discriminate between stages predict outcome response resistance (immuno) therapies (2) therapeutic targets for patients. This review summarizes tumor-mediated escape, discuss potential diagnostic, predictive tools use therapeutics alternative application methods, chimeric receptor T cells.

Language: Английский

Citations

2
Modeling Respiratory Tract Diseases for Clinical Translation Employing Conditionally Reprogrammed Cells DOI Creative Commons
Danyal Daneshdoust, Kai He, Qi-En Wang

et al.

Cell Insight, Journal Year: 2024, Volume and Issue: 3(6), P. 100201 - 100201

Published: Sept. 18, 2024

Language: Английский

Citations

2