Natural monobacterial environments modulate viral infection inCaenorhabditis elegans DOI Creative Commons
Rubén González,

Marie‐Anne Félix

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: June 13, 2023

ABSTRACT The microbes associated with an organism play a pivotal role in modulating their host susceptibility to viral infections. However, the influence of individual on infections is not well understood. Here, we examined impact 67 naturally bacterial associates Caenorhabditis elegans Orsay virus. Our findings reveal that infection C. significantly influenced by monobacterial environments. majority tested natural environments reduced elegan s while some increased compared Escherichia coli environmental reference. reduction caused degradation virions or poor nutrition bacteria. does require known antiviral responses, including RNA interference and transcriptional regulation downstream RIG-I homolog DRH-1. one bacterium, Lelliottia JUb276, but induce resistance drh-1 mutants. research underscores importance considering biotic environment studies provides insights for future host-microbes-virus interactions immunity. AUTHOR CONTRIBUTIONS Conceptualization: RG, MAF; Formal analysis: RG; Investigation: Resources: Data Curation: Writing - Original Draft: Review & Editing: Visualization: Supervision: Project administration: Funding acquisition: MAF.

Language: Английский

Role of government policy in the relationship between charismatic leadership and entrepreneurship in the Kurdistan Region of Iraq SMEs DOI Creative Commons
Rekurd S. Maghdid,

Abdulqader Abdulameer Hasan,

Aso K. Omer

et al.

Discover Sustainability, Journal Year: 2025, Volume and Issue: 6(1)

Published: May 4, 2025

Language: Английский

Citations

0
Experimental evolution of an RNA virus inCaenorhabditis elegans DOI Creative Commons
Victoria G. Castiglioni, María J. Olmo-Uceda,

Susana Martín

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 14, 2024

ABSTRACT The discovery of Orsay virus (OrV), the first infecting wild populations Caenorhabditis elegans , has boosted studies viral immunity pathways in this nematode. Considering many advantages that C. offers for fundamental research host-pathogen interactions, pathosystem high potential to become a model system experimental evolution studies. However, evolutionary constraints operating have barely been explored. Here we describe time an experiment two different OrV strains . After 10 serial passages evolution, report slight changes infectivity and non-synonymous mutations fixed evolved populations. In addition, observed numerous minor variants emerging population. These were not randomly distributed along genome but concentrated polymorphic genomic regions. Overall, our work established grounds future using nematodes. HIGHLIGHTS -Orsay is convenient study evolution. approach used test may interfere with infection phenotypes observed. There be specific hotspots regions nucleotide diversity important virus. substitution rate was low, suggesting studied might already well adapted laboratory conditions.

Language: Английский

Citations

1
Story of an infection: Viral dynamics and host responses in the Caenorhabditis elegans –Orsay virus pathosystem DOI Creative Commons
Victoria G. Castiglioni, María J. Olmo-Uceda, Ana Villena-Giménez

et al.

Science Advances, Journal Year: 2024, Volume and Issue: 10(39)

Published: Sept. 27, 2024

Orsay virus (OrV) is the only known natural affecting Caenorhabditis elegans , with minimal impact on animal’s fitness due to its robust innate immune response. This study aimed understand interactions between C. and OrV by tracking infection’s progression during larval development. Four distinct stages of infection were identified basis viral load, a peak in capsid-encoding RNA2 coinciding first signs egression. Transcriptomic analysis revealed temporal changes gene expression functions induced infection. A specific set up-regulated genes remained active throughout infection, correlated anticorrelated accumulation identified. Responses mirrored reactions other biotic stressors, distinguishing virus-specific responses broader responses. Moreover, mutants early response defense-related processes showed altered load progression, uncovering additional players antiviral defense

Language: Английский

Citations

1
Experimental evolution of an RNA virus in Caenorhabditis elegans DOI Creative Commons
Victoria G. Castiglioni, María J. Olmo-Uceda,

Susana Martín

et al.

Infection Genetics and Evolution, Journal Year: 2024, Volume and Issue: 123, P. 105623 - 105623

Published: June 18, 2024

The discovery of Orsay virus (OrV), the first infecting wild populations Caenorhabditis elegans, has boosted studies viral immunity pathways in this nematode. Considering many advantages that C. elegans offers for fundamental research host-pathogen interactions, pathosystem high potential to become a model system experimental evolution studies. However, evolutionary constraints - i.e, balance between genetic variation, selection, drift and historical contingency- operating have barely been explored. Here we describe time an experiment two different OrV strains elegans. Comparison ancestral showed differences infectivity sequence, highlighted importance consistently normalize inocula meaningful comparisons among strains. After 10 serial passages evolution, report slight changes non-synonymous mutations fixed evolved populations. In addition, observed numerous minor variants emerging population. These were not randomly distributed along genome but concentrated polymorphic genomic regions. Overall, our work established grounds future using nematodes.

Language: Английский

Citations

0
Natural monobacterial environments modulate viral infection inCaenorhabditis elegans DOI Creative Commons
Rubén González,

Marie‐Anne Félix

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: June 13, 2023

ABSTRACT The microbes associated with an organism play a pivotal role in modulating their host susceptibility to viral infections. However, the influence of individual on infections is not well understood. Here, we examined impact 67 naturally bacterial associates Caenorhabditis elegans Orsay virus. Our findings reveal that infection C. significantly influenced by monobacterial environments. majority tested natural environments reduced elegan s while some increased compared Escherichia coli environmental reference. reduction caused degradation virions or poor nutrition bacteria. does require known antiviral responses, including RNA interference and transcriptional regulation downstream RIG-I homolog DRH-1. one bacterium, Lelliottia JUb276, but induce resistance drh-1 mutants. research underscores importance considering biotic environment studies provides insights for future host-microbes-virus interactions immunity. AUTHOR CONTRIBUTIONS Conceptualization: RG, MAF; Formal analysis: RG; Investigation: Resources: Data Curation: Writing - Original Draft: Review & Editing: Visualization: Supervision: Project administration: Funding acquisition: MAF.

Language: Английский

Citations

0