Systemic and local cues drive neural stem cell niche remodelling during neurogenesis in Drosophila DOI Creative Commons
Pauline Spéder, Andrea H. Brand

eLife, Journal Year: 2018, Volume and Issue: 7

Published: Jan. 4, 2018

Successful neurogenesis requires adequate proliferation of neural stem cells (NSCs) and their progeny, followed by neuronal differentiation, maturation survival. NSCs inhabit a complex cellular microenvironment, the niche, which influences behaviour. To ensure sustained neurogenesis, niche must respond to extrinsic, environmental changes whilst fulfilling intrinsic requirements neurogenic program adapting roles accordingly. However, very little is known about how different adjust properties such inputs. Here, we show that nutritional NSC-derived signals induce remodelling Drosophila cortex glia, this glial evolving needs NSCs. First, nutrition-induced activation PI3K/Akt drives glia expand membrane processes. Second, when emerge from quiescence resume proliferation, they signal promote formation bespoke structure around each NSC lineage. The remodelled essential for newborn neuron

Language: Английский

Antioxidant Role for Lipid Droplets in a Stem Cell Niche of Drosophila DOI Creative Commons
Andrew P. Bailey, Grielof Koster, Christelle Guillermier

et al.

Cell, Journal Year: 2015, Volume and Issue: 163(2), P. 340 - 353

Published: Oct. 1, 2015

Stem cells reside in specialized microenvironments known as niches. During Drosophila development, glial provide a niche that sustains the proliferation of neural stem (neuroblasts) during starvation. We now find cell also preserves neuroblast under conditions hypoxia and oxidative stress. Lipid droplets form glia stress limit levels reactive oxygen species (ROS) inhibit oxidation polyunsaturated fatty acids (PUFAs). These protect neuroblasts from peroxidation chain reactions can damage many types macromolecules. The underlying antioxidant mechanism involves diverting PUFAs, including diet-derived linoleic acid, away membranes to core lipid droplets, where they are less vulnerable peroxidation. This study reveals an role for could be relevant different biological contexts.

Language: Английский

Citations

590
Proliferation control in neural stem and progenitor cells DOI
Catarina C. F. Homem,

Marko Repic,

Juergen A. Knoblich

et al.

Nature reviews. Neuroscience, Journal Year: 2015, Volume and Issue: 16(11), P. 647 - 659

Published: Sept. 30, 2015

Language: Английский

Citations

349
Overcoming blood–brain barrier transport: Advances in nanoparticle-based drug delivery strategies DOI
Shichao Ding,

Aminul Islam Khan,

Xiaoli Cai

et al.

Materials Today, Journal Year: 2020, Volume and Issue: 37, P. 112 - 125

Published: March 4, 2020

Language: Английский

Citations

320
Glial Glycolysis Is Essential for Neuronal Survival in Drosophila DOI Creative Commons

Anne Volkenhoff,

Astrid Weiler,

Matthias C. Letzel

et al.

Cell Metabolism, Journal Year: 2015, Volume and Issue: 22(3), P. 437 - 447

Published: July 30, 2015

Language: Английский

Citations

305
When bigger is better: the role of polyploidy in organogenesis DOI
Terry L. Orr‐Weaver

Trends in Genetics, Journal Year: 2015, Volume and Issue: 31(6), P. 307 - 315

Published: April 26, 2015

Language: Английский

Citations

267
The glia of the adult Drosophila nervous system DOI Creative Commons

Malte C. Kremer,

Christophe Jung,

Sara Batelli

et al.

Glia, Journal Year: 2017, Volume and Issue: 65(4), P. 606 - 638

Published: Jan. 30, 2017

Glia play crucial roles in the development and homeostasis of nervous system. While GLIA Drosophila embryo have been well characterized, their study adult system has limited. Here, we present a detailed description glia system, based on analysis some 500 glial drivers identified within collection synthetic GAL4 lines. We find that make up ∼10% cells envelop all compartments neurons (soma, dendrites, axons) as whole. Our morphological suggests set simple rules governing morphogenesis interactions with other cells. All subtypes minimize contact neighbors but maximize adapt macromorphology micromorphology to neuronal entities they envelop. Finally, show no obvious spatial organization or registration entities. regional specializations, together powerful genetic toolkit provide, will facilitate functional mature 2017 2017;65:606–638

Language: Английский

Citations

261
A Circadian Clock in the Blood-Brain Barrier Regulates Xenobiotic Efflux DOI Creative Commons
Shirley Zhang,

Zhifeng Yue,

Denice M. Arnold

et al.

Cell, Journal Year: 2018, Volume and Issue: 173(1), P. 130 - 139.e10

Published: March 1, 2018

Language: Английский

Citations

226
The Drosophila blood-brain barrier: development and function of a glial endothelium DOI Creative Commons

Stéfanie Limmer,

Astrid Weiler,

Anne Volkenhoff

et al.

Frontiers in Neuroscience, Journal Year: 2014, Volume and Issue: 8

Published: Nov. 14, 2014

The efficacy of neuronal function requires a well-balanced extracellular ion homeostasis and steady supply with nutrients metabolites. Therefore, all organisms equipped complex nervous system developed so-called blood-brain barrier, protecting it from an uncontrolled entry solutes, metabolites or pathogens. In higher vertebrates, this diffusion barrier is established by polarized endothelial cells that form extensive tight junctions, whereas in lower vertebrates invertebrates the exclusively formed glial cells. Here, we review development Drosophila melanogaster. system, at least seven morphologically distinct cell classes can be distinguished. Two these barrier. Perineurial participate nutrient uptake establish first subperineurial septate which block paracellular thus seal hemolymph. We summarize molecular basis junction formation address different transport systems expressed forming

Language: Английский

Citations

193
Dilp8 requires the neuronal relaxin receptor Lgr3 to couple growth to developmental timing DOI Creative Commons
Andrés Garelli, Fabiana Herédia, Andreia P. Casimiro

et al.

Nature Communications, Journal Year: 2015, Volume and Issue: 6(1)

Published: Oct. 29, 2015

How different organs in the body sense growth perturbations distant tissues to coordinate their size during development is poorly understood. Here we mutate an invertebrate orphan relaxin receptor gene, Drosophila Leucine-rich repeat-containing G protein-coupled 3 (Lgr3), and find asymmetries similar those found insulin-like peptide 8 (dilp8) mutants, which fail with developmental timing. Indeed, mutation or RNA intereference (RNAi) against Lgr3 suppresses delay pupariation induced by imaginal disc perturbation ectopic Dilp8 expression. By tagging endogenous performing cell type-specific RNAi, map this activity a new subset of CNS neurons, four are pair bilateral pars intercerebralis Lgr3-positive (PIL) neurons that respond specifically increasing cAMP-dependent signalling. Our work sheds light on function evolution receptors reveals novel neuroendocrine circuit responsive aberrations.

Language: Английский

Citations

175
Drosophila glia: Few cell types and many conserved functions DOI Open Access
Kerem Yildirim,

Johanna Petri,

Rita Kottmeier

et al.

Glia, Journal Year: 2018, Volume and Issue: 67(1), P. 5 - 26

Published: Nov. 15, 2018

Abstract Glial cells constitute without any dispute an essential element in providing efficiently operating nervous system. Work many labs over the last decades has demonstrated that neuronal function, from action potential generation to its propagation, eliciting synaptic responses subsequent postsynaptic integration, is evolutionarily highly conserved. Likewise, biology of glial appears conserved core elements and therefore, a deeper understanding expected benefit analyzing model organisms such as Drosophila melanogaster . particularly well suited for studying since fly system only limited number exists, which can be individually identified based on position set molecular markers. In combination with well‐known genetic tool box unprecedented level analysis feasible, not help identify novel molecules principles governing cell function but also will better understand functions first mammalian Here we review current knowledge glia spark interest using this analyze complex traits future.

Language: Английский

Citations

160