Co-Exposure to Ammonia and Lipopolysaccharide-Induced Impaired Energy Metabolism via the miR-1599/HK2 Axis and Triggered Autophagy, ER Stress, and Apoptosis in Chicken Cardiomyocytes

Poultry Science, Journal Year: 2025, Volume and Issue: 104(4), P. 104965 - 104965

Published: March 1, 2025

Ammonia (NH3) and lipopolysaccharide (LPS), common pollutants in poultry farming environments, pose significant health risks by disrupting cellular processes. Although previous studies have demonstrated the individual effect of NH3 or LPS on human animal health, mechanisms underlying their combined impact chicken heart tissue remain poorly understood. In this study, we established a cardiotoxicity model to investigate effects and/or exposure energy metabolism, autophagy, endoplasmic reticulum (ER) stress, apoptosis cardiomyocytes. Our findings indicated that or/and reduced ATPase activity ATP content, led downregulation HK2, PK, PDHX, SDH, upregulation AMPK, resulting impaired metabolism Additionally, found gga-miR-1599/HK2 axis as key regulator involved LPS-induced impairment. The impairment activated AMPK/mTOR pathway, which subsequently triggered evidenced Beclin, LC3-I, LC3-II. Furthermore, decreased mTOR expression induced ER markers such ATF6, GRP78, IRE1, PERK. turn, increased CHOP expression, downregulated Bcl-2 upregulated Bim, elevated levels Bax, caspase-9, caspase-3, ultimately triggering apoptosis. This study provides valuable insights into co-exposure identifies potential molecular targets for mitigating these adverse effects.

Language: Английский

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Polyhalogenated carbazoles (PHCZs) induce cardiotoxicity and behavioral changes in zebrafish at early developmental stages

The Science of The Total Environment, Journal Year: 2022, Volume and Issue: 841, P. 156738 - 156738

Published: June 15, 2022

Language: Английский

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Deterioration of muscle quality caused by ammonia exposure in rainbow trout (Oncorhynchus mykiss)

Food Bioscience, Journal Year: 2023, Volume and Issue: 53, P. 102609 - 102609

Published: March 30, 2023

Language: Английский

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Critical Knowledge Gaps and Future Priorities Regarding the Intestinal Barrier Damage After Traumatic Brain Injury

World Neurosurgery, Journal Year: 2024, Volume and Issue: 188, P. 136 - 149

Published: May 22, 2024

Language: Английский

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The protective effect of Luteolin on chicken spleen lymphocytes from ammonia poisoning through mitochondria and balancing energy metabolism disorders

Poultry Science, Journal Year: 2023, Volume and Issue: 102(12), P. 103093 - 103093

Published: Sept. 9, 2023

Ammonia poses a significant challenge in the contemporary intensive breeding industry, resulting substantial economic losses. Despite this, there is dearth of research investigating efficacious strategies to prevent ammonia poisoning poultry. Consequently, objective this study was investigate molecular mechanisms through which Luteolin (Lut) safeguards mitochondria and restores equilibrium energy metabolism disorders, thereby shielding chicken spleen lymphocytes from detrimental effects poisoning. Chicken were categorized into 3 distinct groups: control group, group (with addition 1 mmol/L ammonium chloride), Lut treatment 0.5 μg/mL for 12 h followed by chloride). These groups then cultured duration 24 h. To potential protective effect on exposed ammonia, various techniques employed, including CCK-8 analysis, ultrastructural observation, reagent kit methodology, fluorescence microscopy, quantitative real-time PCR (qRT-PCR). The findings indicate that has mitigate morphological damage caused Additionally, it can counteract decline mitochondrial membrane potential, ATP content, ATPase activities (specifically Na+/K+-ATPase, Ca2+-ATPase, Mg2+-ATPase, Ca/Mg2+-ATPase) following exposure lymphocytes. also ability regulate expression genes involved fusion (Opa1, Mfn1, Mfn2) division (Drp1 Mff) after exposure. This regulation leads balanced (HK1, HK2, LDHA, LDHB, PFK, PK, SDHB, ACO2) provides protection against

Language: Английский

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Trends and recent progresses of selenium nanoparticles as novel autophagy regulators for therapeutic development

Frontiers in Nutrition, Journal Year: 2023, Volume and Issue: 10

Published: Feb. 2, 2023

Autophagy, one of the major intracellular degradation systems, plays an important role in maintaining normal cellular physiological functions and protecting organisms from different diseases. Selenium (Se), essential trace element, is involved many metabolic regulatory signaling events a key human health. In recent years, selenium nanoparticles (Se NPs) have attracted increasing attentions biomedical field due to their low toxicity, high bioavailability bioactivity. Taking advantage advanced biological activities, Se NPs can be used alone as potential therapeutic agents, or combine with other agents served carriers for development novel therapeutics. More interestingly, been widely reported affect autophagy signaling, which therefor allow against Here, this review suggested relationships between autophagy, followed by trends progresses regulation diseased conditions. importantly, work discussed roles mechanisms regulating, might enhance our understanding about how regulate disease treatment. This expected promote application regulators, benefit associated

Language: Английский

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Sirtuin 5 alleviates apoptosis and autophagy stimulated by ammonium chloride in bovine mammary epithelial cells

Experimental and Therapeutic Medicine, Journal Year: 2024, Volume and Issue: 28(1)

Published: May 22, 2024

Ammonia (NH3) is an irritating and harmful gas that affects cell apoptosis autophagy. Sirtuin 5 (SIRT5) has multiple enzymatic activities regulates NH3‑induced autophagy in tumor cells. In order to determine whether SIRT5 bovine mammary epithelial autophagy, cells with overexpression or knockdown were generated addition, treated inhibitors. The results showed reduced the content of NH3 glutamate by inhibiting glutaminase activity glutamine metabolism, ratio ADP/ATP. inhibitor groups comparable, including increased activating activity, elevated It was further confirmed inhibited through reverse transcription‑quantitative PCR, western blot, flow cytometry Annexin V FITC/PI staining transmission electron microscopy. it also found addition LY294002 Rapamycin PI3K/Akt mTOR kinase signal, decreasing induced SIRT5‑inhibited NH3. summary, PI3K/Akt/mTOR signal involved relies on regulation SIRT5. This study provides a new theory for use regulate guidance improving health production performance dairy cows.

Language: Английский

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Cross-Talk Between Selenium Nanoparticles and Cancer Treatment Through Autophagy

Biological Trace Element Research, Journal Year: 2023, Volume and Issue: 202(7), P. 2931 - 2940

Published: Oct. 10, 2023

Language: Английский

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Taurine inhibits acute ammonia-induced oxidative stress, immune response, and apoptosis of large yellow croaker (Larimichthys crocea) during keep-live transport

Journal of Agriculture and Food Research, Journal Year: 2025, Volume and Issue: 19, P. 101716 - 101716

Published: Feb. 6, 2025

Language: Английский

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The use of selenomethionine to reduce ammonia toxicity in porcine spleen by inhibiting endoplasmic reticulum stress and autophagy mediated by oxidative stress

Ecotoxicology and Environmental Safety, Journal Year: 2022, Volume and Issue: 242, P. 113887 - 113887

Published: July 15, 2022

Language: Английский

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