Transgenerational Reproductive and Developmental Toxicity Induced by N‐Nitrosodimethylamine and Its Metabolite Formaldehyde in Drosophila melanogaster

Journal of Applied Toxicology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 7, 2025

ABSTRACT N ‐Nitrosodimethylamine (NDMA) is a known water disinfection byproduct (DBP) characterized as potent hepatotoxin, promutagen, and probable human carcinogen; this because of the metabolites associated with its biotransformation. The metabolism NDMA produces formaldehyde, another alkylating agent DBP. Both compounds are generated from natural anthropogenic sources, but safety restrictions applied to do not extend uses formaldehyde. Hence, potential health ecological risks concern. Due limited information on long‐term effects exposure these at environmentally relevant concentrations, work aimed compare transgenerational reproductive developmental toxicity separate exposures or metabolite formaldehyde in Drosophila melanogaster over four generations. parental flies were fed (1.19E−06 5 mM) for 48 h during third larval instar. Subsequent offspring (F1–F3) grown under compound‐free conditions. In generation, both modified time emergence reduced number progenies. NDMA, was lethal, affected fertility, weakly induced malformations. next generations, malformed offspring. Reproductive malformations maintained least three suggesting that detrimental could unexposed This first study reporting individual reproduction development between D. , highlighting relevance evaluating multiple generations accurately determine environmental pollutants.

Language: Английский

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Risk assessment of heavy metals in agricultural soil based on the coupling model of Monte Carlo simulation-triangular fuzzy number

Environmental Geochemistry and Health, Journal Year: 2024, Volume and Issue: 46(2)

Published: Jan. 31, 2024

Language: Английский

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Epigenetic inheritance and gene expression regulation in early Drosophila embryos

EMBO Reports, Journal Year: 2024, Volume and Issue: 25(10), P. 4131 - 4152

Published: Sept. 16, 2024

Precise spatiotemporal regulation of gene expression is paramount importance for eukaryotic development. The maternal-to-zygotic transition (MZT) during early embryogenesis in Drosophila involves the gradual replacement maternally contributed mRNAs and proteins by zygotic products. genome transcriptionally activated first 3 hours development, a process known as "zygotic activation" (ZGA), orchestrated activities few pioneer factors. Their decisive role ZGA has been characterized detail, whereas contribution chromatin factors to this historically overlooked. In review, we aim summarize current knowledge how impacts stages embryonic particular, will address following questions: affect transcriptional silencing, architecture promotes integration these processes Remarkably, certain marks can be intergenerationally inherited, their presence embryo becomes critical at later stages. Finally, speculate on possible roles carriers epialleles transgenerational epigenetic inheritance (TEI).

Language: Английский

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Molecular insight into reproductive toxicity and transgenerational effects of Cadmium exposure on Drosophila melanogaster

Ecotoxicology and Environmental Safety, Journal Year: 2025, Volume and Issue: 291, P. 117870 - 117870

Published: Feb. 1, 2025

Cadmium (Cd), a widespread and serious environmental pollutant, has recently garnered increasing scientific scrutiny due to its profound adverse effects. Although the evidence for Cd-induced reproductive toxicity is well established, it remains elusive on intricate dose-response relationship underlying molecular mechanisms, especially transgenerational in animals. Here, we employed fruit fly (Drosophila melanogaster) as model organism examine performance across five generations by parental exposure varying concentrations of Cd (5, 50, 500 μM). Firstly, our observations number eggs laid, pupae formed, adult flies emerged directly exposed generation (F0) confirmed dose-dependent decline fecundity. Transcriptome analysis revealed that, oxidative stress ion transport disruption F0 could underlie synaptic dysfunction impaired follicle cell development, impacting behavior oocyte fertility. Employing analysis, Wnt signaling pathway mTOR were identified early responses toxicity. Secondly, sustained detrimental effects observed at least two three after removal. At epigenetic level, perturb fecundity modulating Dnmt2 expression, pivotal regulator methylation processes. Moreover, despite phenotypic recovery F4, persistent changes indicate enduring toxicity, highlighting need vigilance against contamination long-term Collectively, findings enhance understanding effects, highlight further improve assessment multigenerational consequences contamination.

Language: Английский

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Polycomb-mediated transgenerational epigenetic inheritance of Drosophila eye colour is independent of small RNAs

Open Biology, Journal Year: 2025, Volume and Issue: 15(3)

Published: March 1, 2025

Transgenerational epigenetic inheritance (TEI) describes the process whereby distinct states are transmitted between generations, resulting in heritable gene expression and phenotypic differences that independent of DNA sequence variation. Chromatin modifications have been demonstrated to be important TEI; however, extent which they require other signals establish maintain is still unclear. Here we investigate whether small non-coding RNAs contribute different Fab2L transgene Drosophila triggered by transient long-range chromosomal contacts, requires Polycomb complex activity deposit H3K27me3 modification for long-term TEI. By analysing mutants deficient RNAs, high-throughput sequencing data, contacts expression, demonstrate do not directly initiation or maintenance silencing. However, uncover an indirect role microRNA silencing through effects on group Pleiohomeotic . Additionally, show a commonly used marker gene, Stubble ( Sb ), affects may interpreting experiments assaying function development. ruling out plausible candidate TEI at transgene, our work highlights variability modes across species.

Language: Английский

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The role of epigenetics in women’s reproductive health: the impact of environmental factors

Frontiers in Endocrinology, Journal Year: 2024, Volume and Issue: 15

Published: Sept. 13, 2024

This paper explores the significant role of epigenetics in women's reproductive health, focusing on impact environmental factors. It highlights crucial link between epigenetic modifications-such as DNA methylation and histones post-translational modifications-and health issues, including infertility pregnancy complications. The reviews influence pollutants like PM2.5, heavy metals, endocrine disruptors gene expression through mechanisms, emphasizing need for understanding how dietary, lifestyle choices, exposure to chemicals affect health. Future research directions include deeper investigation into female leveraging editing mitigate changes improving IVF success rates managing disorders.

Language: Английский

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Transcriptional reprogramming in the entomopathogenic fungus Metarhizium brunneum and its aphid host Myzus persicae during the switch between saprophytic and parasitic lifestyles

BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: Oct. 2, 2024

Language: Английский

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An evolutionary perspective of lifespan and epigenetic inheritance

Experimental Gerontology, Journal Year: 2023, Volume and Issue: 179, P. 112256 - 112256

Published: July 17, 2023

In the last decade epigenetics has come to fore as a discipline which is central biogerontology. Age associated epigenetic changes are routinely linked with pathologies, including cardiovascular disease, cancer, and Alzheimer's disease; moreover, clocks capable of correlating biological age chronological in many species humans. Recent intriguing empirical observations also suggest that inherited effects could influence lifespan/longevity variety organisms. If this case, an imperative exists reconcile processes evolution ageing. This review will critically evaluate from evolutionary perspective. The overarching aim integrate evidence suggests inheritance modulates main theories

Language: Английский

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Changes in the wing shape and size in fruit flies exposed to micro and nanoplastics

Chemosphere, Journal Year: 2024, Volume and Issue: 363, P. 142821 - 142821

Published: Sept. 1, 2024

Language: Английский

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An epigenetic change in a moth is generated by temperature and transmitted to many subsequent generations mediated by RNA

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(3), P. e0292179 - e0292179

Published: March 7, 2024

Epigenetic changes in sexually reproducing animals may be transmitted usually only through a few generations. Here we discovered case where epigenetic change lasts 40 This phenomenon occurs the short antennae ( sa ) mutation of flour moth Ephestia kuehniella ). We demonstrate that is probably determined by small RNA (e.g., piRNA, miRNA, tsRNA) and this way to subsequent generations male female gametes. The observed cancels creates wild phenotype (a appears have no mutation). It persists for many (40 recorded). transgenerational effect (suppression homozygous antennae) induced during ontogenetic development, such as increased temperature on pupae food, different salts or injection from sperm already affected individuals into eggs. occasionally disappear some and/or progeny pair generation chain which transfers. consider survival over has adaptive consequences. mainly response environmental offspring via RNA. In study, test an interesting with unexpected length after what its cause. Such transfer greater evolutionary significance than previously thought. Based analogies, also discuss connection SIR2 gene.

Language: Английский

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