Data-Independent Acquisition-Based Quantitative Proteomics Analysis of Fertile Red Eggs and Spermatozoa in Hermatypic Coral Galaxea fascicularis: Revealing Key Proteins Related to Gamete Maturation and Fertilization DOI Creative Commons
Yinyin Zhou,

Jingzhao Ke,

Lishu Zheng

et al.

Journal of Marine Science and Engineering, Journal Year: 2024, Volume and Issue: 12(12), P. 2341 - 2341

Published: Dec. 20, 2024

Sexually propagated scleractinian corals are in high demand for coral reef restoration. However, threatened reef-building corals, many of the molecular mechanisms related to their reproduction remain largely unknown, which forms a major bottleneck large-scale cultivation sexually reproducing corals. In this study, we analyzed proteomic signatures red eggs and spermatozoa from ecologically significant Galaxea fascicularis, using data-independent acquisition mass spectrometry (DIA-MS) method. A total 7741 7279 proteins mature were identified, respectively. Among these proteins, 596 spermatozoa-specific 1056 egg-specific. Additionally, 4413 differentially abundant (DAPs) among 3121 up-regulated 1292 spermatozoa. Furthermore, anenrichment analyses showed that DAPs identified mainly involved progesterone-mediated oocyte maturation pathway lectin pathway; detected insulin secretion metabolic pathways generation energy. This result will contribute discovery intrinsic regulation gamete fertilization. at least 57 associated with including fluorescent protein (RFP), vitellogenin (VG), egg (EP), testis-specific serine/threonine-protein kinase family (TSSKs), EF-hand Ca2+-binding (EFHC1 EFHC2). Particularly, third yolk EUPHY was reported first time G. fascicularis. conclusion, study unveiled groundbreaking insights into sexual reproduction, paving way more effective conservation sustainable development ecosystems

Language: Английский

An analysis of the tripartite evolutionary game for building the ecological security barrier in the Bohai Sea under the threshold of land-sea integration DOI Creative Commons
Di Luan, Hongjun Cao, Tongkun Qu

et al.

Frontiers in Marine Science, Journal Year: 2024, Volume and Issue: 10

Published: Jan. 8, 2024

The construction of marine ecological security barriers (MESBs) is fundamental for the sustainable development environment and economy, an important component strategy land-sea integration. It difficult to build effective MESB simply by relying on power government; therefore, this respect, it urgent encourage multiple participating subjects work together. This study takes Bohai Sea as research object, uses evolutionary game theory construct a model decision-making behavior among enterprises, local governments, public in process Sea, so explore strategic stability equilibrium point through analysis interests three decision makers, lay foundation promoting process. results are follows: (1) Local government regulation key promote transformation from policy documents goal practice; moreover, directly influences whether main sources land-based pollution, i.e., will abandon traditional approach production actively MESB. (2) supervision reporting can be used synergistic, auxiliary means. Thanks government’s publicity education, develop social awareness security, which turn ensure better governments and, thus, active participation enterprises Sea. (3) Participation costs, benefits, corporate image, fines, other factors considered choice with different having marginal effects at stages. Finally, proposes measures further clarify roles functions stakeholders balance subjects, provide implementation path realization “Ocean Power”.

Language: Английский

Citations

6
Survival and growth of “branches of opportunity” from ten coral species outplanted on framed reef modules DOI
Junling Zhang,

Jingzhao Ke,

Xiangbo Liu

et al.

Ecological Engineering, Journal Year: 2025, Volume and Issue: 212, P. 107529 - 107529

Published: Jan. 21, 2025

Language: Английский

Citations

0
Deciphering the environmental adaptation and functional trait of core and noncore bacterial communities in impacted coral reef seawater DOI
Wentao Zhu, He Zhao,

Jingzhao Ke

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 931, P. 172897 - 172897

Published: April 30, 2024

Language: Английский

Citations

3
Framed reef modules: a new and cost‐effective tool for coral restoration DOI Open Access
Xiangbo Liu, Wentao Zhu,

Ruimei Chen

et al.

Restoration Ecology, Journal Year: 2023, Volume and Issue: 32(1)

Published: Aug. 16, 2023

Coral reef ecosystems worldwide are facing significant damage due to climate change and human disturbances. Consequently, restoration actions have been implemented repair degraded areas. However, many efforts hindered by severe environmental conditions, which can hamper success create challenges in developing cost‐effective coral methods. To address this, we conducted a study test low‐cost tool known as “framed modules” (FRMs), specifically aiming mitigate the impacts of typhoons. We measured growth rates (after 300 days) survivorship 400 Acropora hyacinthus A. microphthalma fragments located at different distances from substrates (H0, H10, H30, H45). The FRMs were exposed multiple typhoons, despite harsh they remained undamaged. For comparison, also examined basalt grid plates buried gravel. After days, H45 showed highest survival ( 86% 90%) compared H30 80% 84%), H10 4% 24%), H0 3% 22%). It was found that transplanted corals with height least 30 cm substrate demonstrated resilience against caused macroalgae, turf algae, sedimentation rates. This effectiveness an ecological engineering approach using enhance three‐dimensional complexity restored reefs rehabilitate damaged Furthermore, this method offered reduced costs improved resilience.

Language: Английский

Citations

4
An integrative analysis of proteomics and metabolomics reveals the effects of active restoration on Acropora hyacinthus DOI Open Access
He Zhao, Xiangbo Liu, Junling Zhang

et al.

Journal of Experimental Marine Biology and Ecology, Journal Year: 2023, Volume and Issue: 570, P. 151972 - 151972

Published: Nov. 8, 2023

Language: Английский

Citations

4
Effects of Ecological Restoration Modes on Runoff and Erosion Reduction and Vegetation Restoration of Waste Dump Slopes in Lingwu DOI

Li Wenye,

Ye Jinpeng,

Xiaoping Guo

et al.

Journal of Resources and Ecology, Journal Year: 2023, Volume and Issue: 14(4)

Published: July 17, 2023

In the coal base of Ningdong, there are many ecological problems associated with existing local technologies, such as imperfect technical system, a poor engineering effect, limited generalization value, and lack monitoring evaluation. Based on screening integration technologies in we have designed constructed 14 restoration plots this study. The were composed two replicates for each six modes CK treatment (nothing treatment). These include bag, rod, wire gabion, gravel sand barrier, living barrier wheat straw modes. all slope Yangchangwan waste dump Ningdong. Several indicators selected vegetation growth observation data collection, including erosion amount, runoff depth, richness, coverage, herbal biomass, bush biomass total biomass. Furthermore, TOPSIS (Technique Order Preference by Similarity to Ideal Solution) method was utilized evaluate effects results showed that mode area had best coverage 45%, richness 1.23 an aboveground 0.60 kg m–2. Its 45.16%, 43.02%, 71.43% higher than CK, respectively. model presented least yield, its 133.46 g m–2 which only 26.80% CK. amount 863.32 cm3 m–2, even 50.00% less show gabion three overall.

Language: Английский

Citations

1
Restoring degraded coral colony using two coral transplantation techniques: A case study from Dapeng Bay, Shenzhen, China DOI Open Access
Bo Yang, Huina Zheng,

Zhouping Cui

et al.

Regional Studies in Marine Science, Journal Year: 2023, Volume and Issue: 69, P. 103289 - 103289

Published: Nov. 15, 2023

Language: Английский

Citations

1
Data-Independent Acquisition-Based Quantitative Proteomics Analysis of Fertile Red Eggs and Spermatozoa in Hermatypic Coral Galaxea fascicularis: Revealing Key Proteins Related to Gamete Maturation and Fertilization DOI Creative Commons
Yinyin Zhou,

Jingzhao Ke,

Lishu Zheng

et al.

Journal of Marine Science and Engineering, Journal Year: 2024, Volume and Issue: 12(12), P. 2341 - 2341

Published: Dec. 20, 2024

Sexually propagated scleractinian corals are in high demand for coral reef restoration. However, threatened reef-building corals, many of the molecular mechanisms related to their reproduction remain largely unknown, which forms a major bottleneck large-scale cultivation sexually reproducing corals. In this study, we analyzed proteomic signatures red eggs and spermatozoa from ecologically significant Galaxea fascicularis, using data-independent acquisition mass spectrometry (DIA-MS) method. A total 7741 7279 proteins mature were identified, respectively. Among these proteins, 596 spermatozoa-specific 1056 egg-specific. Additionally, 4413 differentially abundant (DAPs) among 3121 up-regulated 1292 spermatozoa. Furthermore, anenrichment analyses showed that DAPs identified mainly involved progesterone-mediated oocyte maturation pathway lectin pathway; detected insulin secretion metabolic pathways generation energy. This result will contribute discovery intrinsic regulation gamete fertilization. at least 57 associated with including fluorescent protein (RFP), vitellogenin (VG), egg (EP), testis-specific serine/threonine-protein kinase family (TSSKs), EF-hand Ca2+-binding (EFHC1 EFHC2). Particularly, third yolk EUPHY was reported first time G. fascicularis. conclusion, study unveiled groundbreaking insights into sexual reproduction, paving way more effective conservation sustainable development ecosystems

Language: Английский

Citations

0