The Role of Cancer Stem Cell-Derived Exosomes in Cancer Progression DOI Creative Commons
Xueting Li, Xinjian Li, Bin Zhang

et al.

Stem Cells International, Journal Year: 2022, Volume and Issue: 2022, P. 1 - 13

Published: May 4, 2022

Cancer stem cells (CSCs) represent a small portion of tumor with self-renewal ability in tissues and are key factor resistance, recurrence, metastasis. CSCs produce large number exosomes through various mechanisms, such as paracrine autocrine signaling. Studies have shown that CSC-derived (CSC-Exos) carry variety gene mutations specific epigenetic modifications indicative unique cell phenotypes metabolic pathways, enabling exchange information the microenvironment (TME) to promote invasion In addition, CSC-Exos metabolites, especially proteins miRNAs, which can activate signaling pathways further development. dual effects on cancer Due advances liquid biopsy technology for early detection, CSCs-Exos may become an important tool diagnosis therapeutic drug delivery. this article, we will review how exert above based two aspects explore their mechanism action.

Language: Английский

Roles and mechanisms of exosomal non-coding RNAs in human health and diseases DOI Creative Commons
Chen Li, Yu‐Qing Ni, Hui Xu

et al.

Signal Transduction and Targeted Therapy, Journal Year: 2021, Volume and Issue: 6(1)

Published: Nov. 10, 2021

Exosomes play a role as mediators of cell-to-cell communication, thus exhibiting pleiotropic activities to homeostasis regulation. Exosomal non-coding RNAs (ncRNAs), mainly microRNAs (miRNAs), long (lncRNAs), and circular (circRNAs), are closely related variety biological functional aspects human health. When the exosomal ncRNAs undergo tissue-specific changes due diverse internal or external disorders, they can cause tissue dysfunction, aging, diseases. In this review, we comprehensively discuss underlying regulatory mechanisms exosomes in addition, explore current knowledge on roles miRNAs, lncRNAs, circRNAs health diseases, including cancers, metabolic neurodegenerative cardiovascular autoimmune infectious determine their potential implication biomarker identification therapeutic exploration.

Language: Английский

Citations

272
Long non-coding RNAs in the doxorubicin resistance of cancer cells DOI

Saeed Ashrafizaveh,

Milad Ashrafizadeh, Ali Zarrabi

et al.

Cancer Letters, Journal Year: 2021, Volume and Issue: 508, P. 104 - 114

Published: March 23, 2021

Language: Английский

Citations

155
The updated role of exosomal proteins in the diagnosis, prognosis, and treatment of cancer DOI Creative Commons
Xinyi Wang, Jing Huang, Wenjie Chen

et al.

Experimental & Molecular Medicine, Journal Year: 2022, Volume and Issue: unknown

Published: Sept. 22, 2022

Abstract Exosomes are vesicles encompassed by a lipid bilayer that released various living cells. Exosomal proteins encapsulated within the membrane or embedded on surface. As an important type of exosome cargo, exosomal can reflect physiological status parent cell and play essential role in cell–cell communication. regulate tumor development, including tumor-related immune regulation, microenvironment reconstruction, angiogenesis, epithelial–mesenchymal transition, metastasis, etc. The features provide insight into generation, targeting, biological function potential sources markers for cancer diagnosis, prognosis, treatment. Here, we summarize effects biology, latest progress application diagnosis contribution therapeutics vaccines.

Language: Английский

Citations

127
Molecular Characterization of Exosomes for Subtype-Based Diagnosis of Breast Cancer DOI
Ya Cao,

Xiaomeng Yu,

Tianyu Zeng

et al.

Journal of the American Chemical Society, Journal Year: 2022, Volume and Issue: 144(30), P. 13475 - 13486

Published: July 8, 2022

Breast cancer is very heterogeneous and the most frequently diagnosed worldwide, precise therapy targeting specific subtypes may improve survival rates of breast patients. In this study, we designed a biomimetic vesicle by camouflaging catalytic DNA machinery with cell membrane, which enabled molecular classification circulating exosomes for subtype-based diagnosis through homotypic recognition. addition, vesicles specifically targeted fused phenotypic homology manipulated to amplify electrochemical signaling using exosomal RNA as an endogenous trigger. The prepared MCF-7 cell-derived membranes were shown recognize estrogen receptor-positive exhibited low detection limit 557 particles mL–1 microRNA-375 used biomarker. Furthermore, MDA-MB-231 displayed satisfactory performance in analysis triple-negative potential therapeutic target, PD-L1 mRNA, Most importantly, cross-validation experiments confirmed high accuracy selectivity recognition-driven subtyping cancer. When applied clinical samples patients, demonstrated feasibility reliability evaluating features stage-specific monitoring patients because signals showed positive correlation disease progression. Therefore, work provide new ideas personalized treatment throughout whole process.

Language: Английский

Citations

88
Exosome-mediated tumor metastasis: Biology, molecular targets and immuno-therapeutic options DOI

Reza Moshrefiravasjani,

Amin Kamrani,

Nazanin Nazari

et al.

Pathology - Research and Practice, Journal Year: 2024, Volume and Issue: 254, P. 155083 - 155083

Published: Jan. 3, 2024

Language: Английский

Citations

18
Lab-on-a-chip systems for cancer biomarker diagnosis DOI

Canan Özyurt,

İnci Uludağ, Bahar İnce

et al.

Journal of Pharmaceutical and Biomedical Analysis, Journal Year: 2023, Volume and Issue: 226, P. 115266 - 115266

Published: Jan. 21, 2023

Language: Английский

Citations

37
Exosomes in brain diseases: Pathogenesis and therapeutic targets DOI Creative Commons
Qingying Si, Linlin Wu,

Deshui Pang

et al.

MedComm, Journal Year: 2023, Volume and Issue: 4(3)

Published: June 1, 2023

Exosomes are extracellular vesicles with diameters of about 100 nm that naturally secreted by cells into body fluids. They derived from endosomes and wrapped in lipid membranes. involved intracellular metabolism intercellular communication. contain nucleic acids, proteins, lipids, metabolites the cell microenvironment cytoplasm. The contents exosomes can reflect their cells' origin allow observation tissue changes states under disease conditions. Naturally have specific biomolecules act as "fingerprint" parent cells, changed pathological conditions be used biomarkers for diagnosis. low immunogenicity, small size, cross blood-brain barrier. These characteristics make unique engineering carriers. incorporate therapeutic drugs achieve targeted drug delivery. carriers therapy still infancy, but exosome provides a new perspective cell-free therapy. This review discussed relationship occurrence treatment some neuropsychiatric diseases. In addition, future applications diagnosis disorders were evaluated this review.

Language: Английский

Citations

32
Salivary Extracellular MicroRNAs for Early Detection and Prognostication of Esophageal Cancer: A Clinical Study DOI Creative Commons
Kai Li, Yusheng Lin,

Yu Zhou

et al.

Gastroenterology, Journal Year: 2023, Volume and Issue: 165(4), P. 932 - 945.e9

Published: July 1, 2023

Early detection of esophageal squamous cell carcinoma (ESCC) will facilitate curative treatment. We aimed to establish a microRNA (miRNA) signature derived from salivary extracellular vesicles and particles (EVPs) for early ESCC prognostication.Salivary EVP miRNA expression was profiled in pilot cohort (n = 54) using microarray. Area under the receiver operator characteristic curve (AUROC) least absolute shrinkage selector operation regression analyses were used prioritize miRNAs that discriminated patients with controls. Using quantitative reverse transcription polymerase chain reaction, candidates measured discovery 72) lines. The prediction models biomarkers training 342) validated an internal 207) external 226).The microarray analysis identified 7 distinguishing control subjects. Because 1 not always detectable lines, other 6 formed panel. A this panel accurately all-stage (AUROC 0.968) successfully 2 independent cohorts. Importantly, could distinguish early-stage (stage Ⅰ/Ⅱ) subjects 0.969, sensitivity 92.00%, specificity 89.17%) (sensitivity 90.32%, 91.04%) 91.07%, 88.06%) validation Moreover, prognostic based on established efficiently predicted high-risk cases poor progression-free survival overall survival.The EVP-based 6-miRNA can serve as noninvasive risk stratification ESCC. Chinese Clinical Trial Registry, ChiCTR2000031507.

Language: Английский

Citations

32
Extracellular Vesicles as Next-Generation Diagnostics and Advanced Therapy Medicinal Products DOI Open Access
Agnieszka Stawarska, Magdalena Bamburowicz‐Klimkowska, Elise Rundén‐Pran

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(12), P. 6533 - 6533

Published: June 13, 2024

Extracellular vesicles (EVs) hold great promise for clinical application as new diagnostic and therapeutic modalities. This paper describes major GMP-based upstream downstream manufacturing processes EV large-scale production, also focusing on post-processing technologies such surface bioengineering uploading studies to yield novel EV-based diagnostics advanced therapy medicinal products. focuses the quality, safety, efficacy issues of bioengineered drug candidates before first-in-human studies. Because trials involving extracellular are global rise, this encompasses different registered clinical-trial register platforms, with varying levels advancement, highlighting growing interest in EV-related programs. Navigating regulatory affairs EVs poses real challenges, obtaining marketing authorization medicines remains complex due lack specific guidelines discusses state-of-the-art knowledge date products, further research needs safe reliable implementation tools settings. Post-marketing pharmacovigilance products is presented, mainly addressing topics risk assessment management.

Language: Английский

Citations

14
Exosomal miR-151-3p in saliva: A potential non-invasive marker for gastric cancer diagnosis and prognosis modulated by Sijunzi decoction (SJZD) in mice DOI Creative Commons
Ping Yang,

Huijun Lei,

Yue Fu

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(7), P. e29169 - e29169

Published: April 1, 2024

Gastric cancer (GC) is one of the most prominent malignancies that originate in epithelial cells gastric mucosa and main causes cancer-related mortality worldwide. New circulating biomarkers exosomal RNA might have great potential for non-invasive early prognosis GC. Sijunzi Decoction (SJZD) a typical representative formula method benefiting Qi strengthening spleen Traditional Chinese Medicine (TCM). However, effects mechanism SJZD treating GC remain unclear. This study looked GC, explored A model with deficiency syndrome was established nude mice, curative were investigated. Differentially expressed miRNAs plasma saliva exosomes sequenced analyzed. Potential target genes these predicted applied Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) signaling pathway enrichment annotation. Overlapping samples analyzed, qRT-PCR performed verification. miR-151a-3p selected, further determined downregulated from group. The intersected enriched extrinsic apoptotic pathways. significantly ameliorates mouse models, miRNAs, particularly miR-151-3p, be modulated by saliva. miR-151-3p may serve as marker diagnosis prognosis.

Language: Английский

Citations

9