Journal of Controlled Release, Journal Year: 2025, Volume and Issue: unknown, P. 113620 - 113620
Published: March 1, 2025
Language: Английский
Nano Letters, Journal Year: 2023, Volume and Issue: 24(1), P. 1 - 8
Published: Dec. 20, 2023
Extracellular vesicles and lipoproteins are lipid-based biological nanoparticles that play important roles in (patho)physiology. Recent evidence suggests extracellular can interact to form functional complexes. Such complexes have been observed biofluids from healthy human donors various vitro disease models such as breast cancer hepatitis C infection. Lipoprotein components also part of the biomolecular corona surrounds contributes identity. Potential mechanisms relevance vesicle–lipoprotein remain poorly understood. This Review addresses current knowledge interface while drawing on pre-existing liposome interactions with nanoparticles. There is an urgent need for further research lipoprotein–extracellular vesicle interface, which could return mechanistic, therapeutic, diagnostic findings.
Language: Английский
Citations
15
Clinical Chemistry, Journal Year: 2024, Volume and Issue: 70(1), P. 165 - 178
Published: Jan. 1, 2024
Substantial research has been devoted to elucidating the role of extracellular vesicles (EVs) in different hallmarks cancer. Consequently, EVs are increasingly explored as a source cancer biomarkers body fluids. However, heterogeneity EVs, complexity fluids, and diversity methods available for EV analysis, challenge development translation EV-based biomarker assays.
Language: Английский
Citations
5
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(5), P. 2617 - 2617
Published: Feb. 23, 2024
Standard non-melanoma skin cancer (NMSC) treatment involves surgery, recently combined with chemotherapy or immunotherapy in cases of advanced tumors. EVs, including exosomes, are integral to carcinogenesis, and found NMSC releasing mediators impacting tumor progression. Nevertheless, the precise intercellular signaling role NMSC-derived EVs remains unclear. This review aims elucidate their potential diagnosis treatment. systematic encompassed literature searches electronic databases from inception September 2023, based on certain inclusion exclusion criteria, addressing molecular cargo, implications diagnosis, prognosis, NMSC. Key components were identified. Extracellular vesicle (EV) proteins RNA have emerged as diagnostic biomarkers EV-based liquid biopsy. Circular CYP24A1, known for its stability, holds promise a biomarker. Long noncoding RNAs (lincRNA-PICSAR) Desmoglein 2 (DSg2) linked drug resistance, serving prognostic biomarkers. EV being actively investigated delivery agents. In conclusion, this showed that display therapeutic targets Further research is imperative fully comprehend mechanisms explore
Language: Английский
Citations
5
Molecular Biology Reports, Journal Year: 2024, Volume and Issue: 51(1)
Published: March 16, 2024
Language: Английский
Citations
5
Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(15), P. 8304 - 8331
Published: April 8, 2024
Extracellular vesicles (EVs) are nano to-micrometer-sized sacs that released by almost all animal and plant cells act as intercellular communicators transferring their cargos between the source target cells. As a safe scalable alternative to conditioned medium-derived EVs, milk-derived EVs (miEVs) have recently gained great deal of popularity. Numerous studies shown miEVs intrinsic therapeutic actions can treat diseases enhance human health. Additionally, they be used natural drug carriers novel classes biomarkers. However, due complexity milk, successful translation from benchtop bedside still faces several unfilled gaps, especially lack standardized protocols for isolation high-purity miEVs. In this work, comprehensively reviewing bovine studies, we provide an overview current knowledge research on while highlighting challenges enormous promise class theranostics. It is hoped study will pave way clinical applications addressing opportunities.
Language: Английский
Citations
5
Journal of Controlled Release, Journal Year: 2024, Volume and Issue: 372, P. 209 - 220
Published: June 22, 2024
Retinal diseases are the leading cause of blindness, resulting in irreversible degeneration and death retinal neurons. One such cell type, ganglion (RGC), is responsible for connecting retina to rest brain through its axons that make up optic nerve primary lost glaucoma traumatic neuropathy. To date, different therapeutic strategies have been investigated protect RGCs from preserve vision, yet currently available restricted treating neuron loss by reducing intraocular pressure. A major barrier identified these studies drug delivery RGCs, which large part due stability, short duration time at target, low efficiency, undesired off-target effects. Therefore, a system deal with problems needed ensure maximum benefit candidate material. Extracellular vesicles (EV), nanocarriers released all cells, lipid membranes encapsulating RNAs, proteins, lipids. As they naturally shuttle encapsulated compounds between cells communicative purposes, may be exploitable offer opportunities overcome hurdles delivery, including molecular weight, barriers retina, adverse Here, we summarize potential an EV system, discussing their superiorities application target RGCs.
Language: Английский
Citations
4
Frontiers in Nanotechnology, Journal Year: 2025, Volume and Issue: 6
Published: Jan. 22, 2025
Extracellular vesicles (EVs), natural membrane-bound structures released by cells, offer a promising platform for precise and targeted delivery of therapeutic payloads, including drugs nanoparticles. This comprehensive review explores the integration engineered nanomaterials with EVs to advance drug systems. It covers various aspects this integration, techniques synthesizing, loading, characterizing, applying within EVs. Methodologies integrating diverse nanocarriers like nanoparticles, liposomes, quantum dots into are discussed, along characterization methods such as electron microscopy light scattering. Attention is also given considerations biological compatibility, safety assessments, strategies surface modification enhance targeting precision. Highlighting applications, delivery, cancer therapy, vaccine development, underscores potential EV-nanomaterial hybrids leverage EVs’ innate capabilities versatility nanomaterials. However, it addresses challenges scalability, standardization, that must be overcome facilitate clinical translation these biomedical solutions.
Language: Английский
Citations
0
Lab on a Chip, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 1, 2025
This design demonstrates the isolation of extracellular vesicles (EVs) from human plasma, utilizing polymer beads as assay surfaces to capture EV subpopulations and magnetic levitation sort at different outlets.
Language: Английский
Citations
0
International Journal of Nanomedicine, Journal Year: 2025, Volume and Issue: Volume 20, P. 1807 - 1820
Published: Feb. 1, 2025
Background: Quality control (QC) is an important element in ensuring drug substances' safety, efficacy, and quality. The dosing regimen for sEVs can be the form of protein concentration or number particles based on results a series quality controls applied as in-process control. Methods: Wharton's Jelly Mesenchymal Stem Cells (WJMSCs) were isolated from four independent umbilical cord samples characterized following International Society Cellular Therapy (ISCT) guidelines. Small extracellular vesicles (sEVs) separately these WJMSCs using Tangential Flow Filtration (TFF) method per Minimal Information Studies Extracellular Vesicles (MISEV2018) Each concentrated sEV preparation was standardized its purity determined by ratio to concentration. Results: All passed (MSCs) characterization QC tests. Qualitatively, EVs-positive markers (CD63 TSG101) intact bilipid membrane detected all preparations. Quantitatively, particle concentrations revealed that preparations "impure" with < 1.5 × 10 9 particles/μg protein. Albumin co-isolated Conclusion: In short, individual pooled deemed due albumin co-isolation TFF method. For therapeutic development, it essential report EV results. Keywords: control, cord, jelly mesenchymal stem cells, tangential flow filtration, small vesicles,
Language: Английский
Citations
0
Bone Reports, Journal Year: 2022, Volume and Issue: 17, P. 101636 - 101636
Published: Nov. 9, 2022
Mesenchymal stromal cells (MSCs) have been utilized in cell therapy for various diseases. Recent studies demonstrated that extracellular vesicles (EVs) released by MSCs play an important role their therapeutic activities. EVs contain a variety of bioactive molecules such as proteins, messenger RNAs (mRNAs), and micro (miRNAs) modify the function recipient transferring these molecules. Despite promising potential EV substitute MSC therapy, there are challenges need to be addressed clinical success therapy. quality has shown vary from batch preparation preparation. As consistency reproducibility effects rely on EVs, it is necessary establish techniques manufacture scalable amounts with same quality. In this manuscript, we discuss factors affect We then introduce pre-clinical bone/cartilage diseases osteoarthritis, rheumatoid arthritis, osteoporosis.
Language: Английский
Citations
17