DNAzyme Loaded Nano-Niosomes Confer Anti-Cancer Effects in the Human Breast Cancer MCF-7 Cells by Inhibiting Apoptosis, Inflammation, and c-Myc/cyclin D1 DOI

Leila Aghamohseni,

Kavian Barzegarian,

Mohammadreza Ferdowsinia

et al.

Deleted Journal, Journal Year: 2024, Volume and Issue: 48(6), P. 1397 - 1410

Published: Oct. 8, 2024

One of the promising strategies for destroying cancer cells is using small-molecule gene-silencing such as DNAzyme. In current study, we used niosomes carriers to promote drug residence and stability in human breast MCF-7 explore possible molecular mechanisms. Preparing was performed thin-layer hydration method. Shape size were assessed transmission electron microscopy (TEM). Cell cytotoxicity, invasion migration, apoptosis, inflammation apoptosis related genes evaluated by MTT assay, wound healing flow cytometry, Real-time PCR. Our results showed that DNAzyme loaded had more powerful effects against proliferation migration compared free Effects attributed elevation suppressing pro-inflammatory cytokines, down-regulation c-Myc/cyclin D1. conclusion, our study declares possess stronger on than targeting inflammation,

Language: Английский

Enhanced delivery of doxorubicin for breast cancer treatment using pH-sensitive starch/PVA/g-C3N4 hydrogel DOI

Parisa Alipournazari,

Mehrab Pourmadadi, Majid Abdouss

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 265, P. 130901 - 130901

Published: March 13, 2024

Language: Английский

Citations

24
Facile preparation of a pH-sensitive biocompatible nanocarrier based on magnetic layered double hydroxides/Cu MOFs-chitosan crosslinked к-carrageenan for controlled doxorubicin delivery to breast cancer cells DOI

Afsaneh Taghikhani,

Mirzaagha Babazadeh, Soodabeh Davaran

et al.

Colloids and Surfaces B Biointerfaces, Journal Year: 2024, Volume and Issue: 243, P. 114122 - 114122

Published: July 24, 2024

Language: Английский

Citations

11
Curcumin Administration Routes in Breast Cancer Treatment DOI Open Access

B. C. Mayo,

Silvana Penroz,

Keila Torres

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(21), P. 11492 - 11492

Published: Oct. 26, 2024

Breast cancer is a public health concern worldwide, characterized by increasing incidence and mortality rates, requiring novel effective therapeutic strategies. Curcumin bioactive compound extracted from turmeric with several pharmacological activities. multifaceted anticancer agent through mechanisms including the modulation of signaling pathways, inhibition cell proliferation, induction apoptosis, production reactive oxygen species. However, poor water solubility bioavailability curcumin create important barriers in its clinical application. This review elaborates on potential breast treatment, focusing efficacy different administration routes synergistic effects other agents. The intravenous curcumin-loaded nanoparticles significantly improves outcomes compared to oral routes. Innovative formulations, such as nano-emulsifying drug delivery systems, have shown promise enhancing bioavailability. While ensures higher direct action tumor cells, it more invasive expensive than administration. Advancing research treatment essential for improving quality life patients.

Language: Английский

Citations

7
Evaluating a targeted Palbociclib-Trastuzumab loaded smart niosome platform for treating HER2 positive breast cancer cells DOI Creative Commons
Shaghayegh Saharkhiz, Negar Nasri, Nazanin Naderi

et al.

International Journal of Pharmaceutics X, Journal Year: 2024, Volume and Issue: 7, P. 100237 - 100237

Published: March 11, 2024

In this study, we present a targeted and pH-sensitive niosomal (pHSN) formulation, incorporating quantum dot (QD)-labeled Trastuzumab (Trz) molecules for the specific delivery of Palbociclib (Pal) to cells overexpressing human epidermal growth factor receptor 2 (HER2). FTIR analyses confirmed successful preparation pHSNs their bioconjugation. The labeled Trz-conjugated Pal-pHSNs (Trz-Pal-pHSNs) exhibited size approximately 170 nm, displaying spherical shape with neutral surface charge −1.2 mV. Pal encapsulation reached ~86%, release pattern followed two-phase pH-dependent mechanism. MTT assessments demonstrated enhanced apoptosis induction, particularly in HER2-positive cells, by Trz-Pal-pHSNs. Fluorescence imaging further validated internalization particles into cells. conclusion, Trz-Pal-pHSNs emerge as promising platform personalized medicine treatment breast cancer.

Language: Английский

Citations

6
Potential of Natural Phenolic Compounds against Doxorubicin-Induced Chemobrain: Biological and Molecular Mechanisms Involved DOI Creative Commons
Simona Serini, Gabriella Calviello

Antioxidants, Journal Year: 2024, Volume and Issue: 13(4), P. 486 - 486

Published: April 18, 2024

Chemotherapy-induced cognitive impairment or "chemobrain" is a prevalent long-term complication of chemotherapy and one the more devastating. Most studies performed so far to identify dysfunctions induced by antineoplastic chemotherapies have been focused on treatment with anthracyclines, frequently administered breast cancer patients, population that, after treatment, shows high possibility long survival and, consequently, chemobrain development. In last few years, different possible strategies explored prevent reduce anthracycline doxorubicin (DOX), known promote oxidative stress inflammation, which strongly implicated in development this brain dysfunction. Here, we critically analyzed results preclinical from years that evaluated potential phenolic compounds (PheCs), large class natural products able exert powerful antioxidant anti-inflammatory activities, inhibiting DOX-induced chemobrain. Several PheCs belonging classes shown be revert morphological damages deficits associated learning, memory, exploratory behavior. We biological molecular mechanisms suggested future perspectives research area.

Language: Английский

Citations

4
Electrochemical Sensor for the Evaluation of Doxorubicin from Novel Pharmaceutical Formulations and Serum DOI Creative Commons
Alexandra Pusta, Mihaela Tertiş,

Irina Bura

et al.

Chemosensors, Journal Year: 2024, Volume and Issue: 12(4), P. 69 - 69

Published: April 19, 2024

This study focuses on addressing the challenges associated with doxorubicin (DOX), an anthracycline chemotherapeutic widely used in cancer treatment. Despite its efficacy, DOX is linked to severe side effects that limit clinical applications. Novel pharmaceutical formulations aim mitigate these issues, providing better safety profiles. The development of requires analytical methods can accurately and quickly quantify DOX. A cost-effective portable electrochemical sensor for detection was developed utilizing in-house printed carbon electrodes decorated gold nanoparticles. detected using differential pulse voltammetry. demonstrated accurate quantification from novel serum, presenting a dynamic range 1 500 μg/mL low 0.3 μg/mL. method, successfully applied characterize DOX-loaded nanosomes, offers valuable alternative early stages formulation development, reducing costs saving time, while maintaining accuracy.

Language: Английский

Citations

4
Liposomes and Niosomes: New trends and applications in the delivery of bioactive agents for cancer therapy DOI
Helena de Almeida Cerqueira Kodel,

Paria Alizadeh,

Samad Nejad Ebrahimi

et al.

International Journal of Pharmaceutics, Journal Year: 2024, Volume and Issue: unknown, P. 124994 - 124994

Published: Nov. 1, 2024

Language: Английский

Citations

4
The role of HDAC2 inhibition in cardioprotection against doxorubicin-induced myocardial injury DOI Creative Commons
Jinsha Liu,

Wenwen Fu,

Xue Wang

et al.

Frontiers in Cardiovascular Medicine, Journal Year: 2025, Volume and Issue: 12

Published: Feb. 26, 2025

The molecular mechanisms underlying cardioprotection against doxorubicin (DOX)-induced myocardial injury are poorly understood. Histone deacetylase 2 (HDAC2) plays a significant role in oxidative stress, apoptosis, and mitochondrial dysfunction is implicated many human diseases, This study investigated the relationship between HDAC2 expression DOX-induced using vivo rat model of cardiotoxicity vitro experiments with H9c2 cardiomyocytes. was established by administering DOX via intraperitoneal injections. suppressed rats sodium butyrate (SB) Echocardiography measurements were performed at baseline on day 15 post-treatment. euthanized cardiac tissues harvested. tissue samples analyzed hematoxylin eosin H&E staining, immunohistochemistry, Masson Sirius Red TUNEL western blotting to determine status apoptosis. In experiments, cells treated DOX. or transfected shRNA knockdown (shHDAC2). from different groups Rt-qPCR, CCK-8 cell viability assay, cardiomyocyte treatment induced rats. DOX-treated showed significantly higher levels compared corresponding controls. However, inhibition mitigated suggested strong association injury. cells, shHDAC2 alleviated apoptosis enhacing AKT phosphorylation. These findings demonstrated that silencing protected activating PI3K/AKT signaling pathway. Suppressing Therefore, promising therapeutic target for mitigating

Language: Английский

Citations

0
Simultaneous Release of Hydrophilic and Hydrophobic Drugs by a pH-sensitive Bio-carrier Based on Layered Double Hydroxides Decorated with l-serine-chitosan DOI
Soheyla Karimi, Hassan Namazi, Mohammad Aghazadeh

et al.

Journal of Polymers and the Environment, Journal Year: 2025, Volume and Issue: unknown

Published: April 2, 2025

Language: Английский

Citations

0
Genistein Improves the Cytotoxic, Apoptotic, and Oxidative-Stress-Inducing Properties of Doxorubicin in SK-MEL-28 Cancer Cells DOI Creative Commons

Andrea Roman,

Andrei Gheorghe Marius Motoc, Iasmina Marcovici

et al.

Medicina, Journal Year: 2025, Volume and Issue: 61(5), P. 798 - 798

Published: April 25, 2025

Background and Objectives: Cutaneous melanoma (CM) poses a continuous challenge in oncology due to the developing resistance available treatments. Doxorubicin (DOX) is noted as one of most effective chemotherapeutics, although associated toxicity limit its use CM treatment. Consequently, DOX has become promising candidate for combination therapies targeting this neoplasm. Genistein (GEN) gathered significant attention anti-neoplastic properties ability enhance effects against several cancers, yet association remains underexplored CM. Therefore, study investigated therapy regimen comprising GEN terms anti-melanoma activity safety profile. Materials Methods: The vitro experiments were performed on SK-MEL-28 HaCaT cells. Cell viability was determined using MTT assay. morphology confluence inspected microscopically. Nuclear cytoskeletal aspects assessed via immunofluorescence. Apoptosis oxidative stress quantified through caspase intracellular reactive oxygen species (ROS) production, respectively. irritant effect evaluated chorioallantoic membrane. Results: results revealed that 10 µM with (0.5 1 µM) provided augmented cytotoxic events (e.g., reduced cell viability, altered confluence, apoptotic-like impairments nuclear shape network, increased caspases-3/7 -9 activity, elevated ROS) cells, compared individual treatments, exerted strong synergistic interaction. Simultaneously, efficiently surpassed toxic loss, hypertrophy, condensation) In ovo, + treatment classified non-irritant. Conclusions: These findings stand first contributions revealing beneficial therapeutic interplay between at physiologically achievable concentrations resulted anti-tumor cells alleviated keratinocytes.

Language: Английский

Citations

0