Generic solving of physiologically-based kinetic models in support of next generation risk assessment due to chemicals DOI Creative Commons
Sandrine Charles, Ophélia Gestin,

Jérémie Bruset

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: May 1, 2022

Abstract Increasing the confidence in using vitro and silico model-based data to aid chemical risk assessment process is one, if not most, important challenge currently facing regulatory authorities. A particularly crucial concern fully take advantage of scientifically valid Physiologically-Based Kinetic (PBK) models. Nevertheless, assessors remain still unwilling employing PBK models within their daily work. Indeed, are often included current official guidance documents. In addition, most users have limited experience modelling general. So, complexity models, together with a lack evaluation methods performances, certainly contributes under-use practical assessment. This paper proposes an innovative unified framework, both writing equations as matrix ordinary differential (ODE), its exact solving simply expressed products. generic solution allows consider many state-variables needed quantify absorption, distribution, metabolism excretion processes living organisms when exposed substances. model makes possible any compartmentalisation be considered, well all appropriate inter-connections between compartments external medium. We first introduce our intermediate steps from ODE solution. Then we apply this framework bioaccumulation testing, before illustrating concrete use through complementary case studies terms species, compounds complexity. Figure

Language: Английский

A critical review of effect modeling for ecological risk assessment of plant protection products DOI
Floriane Larras, Sandrine Charles, Arnaud Chaumot

et al.

Environmental Science and Pollution Research, Journal Year: 2022, Volume and Issue: 29(29), P. 43448 - 43500

Published: April 7, 2022

Language: Английский

Citations

29
Multiple reaction monitoring mass spectrometry for the discovery of environmentally modulated proteins in an aquatic invertebrate sentinel species, Gammarus fossarum DOI Creative Commons
Maxime Leprêtre, Olivier Geffard,

Anabelle Espeyte

et al.

Environmental Pollution, Journal Year: 2022, Volume and Issue: 315, P. 120393 - 120393

Published: Oct. 9, 2022

Language: Английский

Citations

23
Fish Physiologically Based Toxicokinetic Modeling Approach for In Vitro–In Vivo and Cross-Species Extrapolation of Endocrine-Disrupting Chemicals in Risk Assessment DOI
Ruili Xie, Yiping Xu, Mei Ma

et al.

Environmental Science & Technology, Journal Year: 2024, Volume and Issue: 58(8), P. 3677 - 3689

Published: Feb. 14, 2024

High-throughput in vitro assays combined with vitro–in vivo extrapolation (IVIVE) leverage responses to predict the corresponding exposures and thresholds of concern. The integrated approach is also expected offer potential for efficient tools provide estimates chemical toxicity various wildlife species instead animal testing. However, developing fish physiologically based toxicokinetic (PBTK) models IVIVE ecological applications challenging, especially plausible estimation an internal effective dose, such as equivalent concentration (FEC). Here, a PBTK model linked was established, parameter optimization unbound fraction, pH-dependent ionization hepatic clearance, integration temperature effect growth dilution. PBTK–IVIVE provides not only more precise tissue-specific concentrations but reasonable approximation FEC targeting estrogenic potency endocrine-disrupting chemicals. Both predictions were compared data accurate most indissociable/dissociable Furthermore, can help determine cross-species variability sensitivity among five species. Using available IVIVE-derived target pathways helpful develop predicted no-effect chemicals similar mode action support screening-level risk assessment.

Language: Английский

Citations

6
Organ-specific accumulation of cadmium and zinc in Gammarus fossarum exposed to environmentally relevant metal concentrations DOI
Ophélia Gestin, Christelle Lopes, Nicolas Delorme

et al.

Environmental Pollution, Journal Year: 2022, Volume and Issue: 308, P. 119625 - 119625

Published: June 14, 2022

Language: Английский

Citations

20
Tracking Nano- and Microplastics Accumulation and Egestion in a Marine Copepod by Novel Fluorescent AIEgens: Kinetic Modeling of the Rhythm Behavior DOI
Zipei Dong, Wen‐Xiong Wang

Environmental Science & Technology, Journal Year: 2023, Volume and Issue: 57(49), P. 20761 - 20772

Published: Nov. 29, 2023

Nano- and microplastics (NMPs) are now prevalent in the marine environment. This study quantified uptake depuration kinetics of spherical polystyrene NMPs different particle sizes (200 nm/30 μm) functional groups (−NH2/–COOH) a temperate calanoid copepod Calanus sinicus (C. sinicus), which exhibited rhythmic feeding patterns natural environments. Aggregated-induced emission (AIE) fluorescent probes were employed to track quantify with excellent photostability biocompatibility. The results showed that C. consumed all types, preference small size amino group. Increased diatom concentrations also inhibited bioaccumulation NMPs. Influenced by behavior, was nonstationary during 6 h phase. After 1–3 rapid uptake, body burden peaked then slowly declined. During 3 phase, rapidly efficiently removed mean half-life only 0.23 h. To further under influence biokinetic model established, Markov chain Monte Carlo method used estimate parameter distribution. Our highlighted copepods unique behavior environmentally relevant exposure, may bioaccumulation, trophic transfer, environmental fate

Language: Английский

Citations

11
Cadmium tolerance is associated with tissue-specific plasticity of metallothionein gene expression in Gammarus fossarum field populations DOI Creative Commons
Auréline Lalouette, Arnaud Chaumot,

Louveline Lepeule

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 13, 2025

Abstract The metallothionein gene family codes for proteins involved in metal homeostasis and acute detoxification of non-essential toxic ions across the tree life. We have previously documented increased cadmium (Cd) tolerance field populations crustacean Gammarus fossarum exposed to chronic metallic contamination geochemical origin. This is lost during maintenance organisms laboratory, transmitted offspring via parental effects. study investigated whether expression Cd-responsive mt1 could be related Cd-tolerance plasticity G. . In eleven with different Cd exposure history, we simultaneously assessed (mortality tests) levels by RT-qPCR gills caeca adult males neonates. two organs were correlated a loss was observed parallel decreased after uncontaminated water. also recorded greater inducibility tolerant laboratory when re-exposed along bi-parental transmission Cd-tolerance. These results suggest that control gammarid histories exposure. Highlights - hepatopancreatic associated populations. Plasticity tolerance. Parental effect supported transgenerational expression, underlid an induction

Language: Английский

Citations

0
Cadmium tolerance is associated with tissue-specific plasticity of metallothionein gene expression in Gammarus fossarum field populations DOI Creative Commons
Auréline Lalouette, Arnaud Chaumot,

Louveline Lepeule

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 22, 2025

Abstract The metallothionein gene family codes for proteins involved in metal homeostasis and acute detoxification of non-essential toxic ions across the tree life. We have previously documented increased cadmium (Cd) tolerance field populations crustacean Gammarus fossarum exposed to chronic metallic contamination geochemical origin. This is lost during maintenance organisms laboratory, transmitted offspring via parental effects. study investigated whether expression Cd-responsive mt1 could be related Cd-tolerance plasticity G. fossarum. In eleven with different Cd exposure history, we simultaneously assessed (mortality tests) levels by RT-qPCR gills caeca adult males neonates. two organs were correlated a loss was observed parallel decreased after uncontaminated water. also recorded greater inducibility tolerant laboratory when re-exposed along bi-parental transmission Cd-tolerance. These results suggest that control gammarid histories exposure.

Language: Английский

Citations

0
In Silico Methods for Environmental Risk Assessment: Principles, Tiered Approaches, Applications, and Future Perspectives DOI
Maria Chiara Astuto, Matteo Riccardo Di Nicola, José Tarazona

et al.

Methods in molecular biology, Journal Year: 2022, Volume and Issue: unknown, P. 589 - 636

Published: Jan. 1, 2022

Language: Английский

Citations

15
Bioaccumulation, organotropism and fate of cadmium in Gammarus fossarum exposed through dietary pathway DOI Creative Commons
Ophélia Gestin, Olivier Geffard, Nicolas Delorme

et al.

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 480, P. 135965 - 135965

Published: Sept. 26, 2024

Language: Английский

Citations

2
Influence of the exposure concentration of dissolved cadmium on its organotropism, toxicokinetic and fate in Gammarus fossarum DOI Creative Commons
Ophélia Gestin, Thomas Lacoue‐Labarthe, Nicolas Delorme

et al.

Environment International, Journal Year: 2022, Volume and Issue: 171, P. 107673 - 107673

Published: Dec. 5, 2022

Information on the relationship between exposure concentrations of metals and their biodistribution among organs remained scarce in invertebrates. The objective this study was to investigate effects Cd concentration organotropism, toxico-kinetic fate metal different gammarids exposed dissolved radioisotope 109Cd. Gammarids male were for 7 days three environmental (i.e. 4, 52 350 ng.L-1) before being placed depuration conditions uncontaminated water). At several sampling times, determined by 109Cd γ-counting water, caeca, cephalon, gills, intestine remaining tissues. Bioconcentration Factors (BCF) relative proportions calculated assess effect bioaccumulation capacities. dependance organ-specific kinetic parameters water estimated fitting nested one-compartment (TK) models both accumulation data, Bayesian inference. Then, each concentrations, exchanges over time formalized a multi-compartments TK model fitted all organ data simultaneously. Our results highlighted that, at end phase, BCF proportions, organ, not significantly modulated concentrations. Kinetically, rates (except intestines) depended concentration, but elimination rates. vivo management organs) qualitatively unchanged according concentration. All these also key role that Cd: bioconcentration storage gills main entry pathway intestine. This shows interest implementing approaches test factors bioaccumulation, inter-organ contaminants invertebrate body enhance understanding toxicity risk.

Language: Английский

Citations

11