The active components and potential mechanisms of Wuji Wan in the treatment of ethanol-induced gastric ulcer: An integrated metabolomics, network pharmacology and experimental validation DOI Creative Commons

Tiantai Wu,

Huan Zhang, Yang Jin

et al.

Journal of Ethnopharmacology, Journal Year: 2024, Volume and Issue: 326, P. 117901 - 117901

Published: Feb. 8, 2024

Wuji Wan (WJW) is a traditional Chinese medicine formula that can be found in the "Prescriptions of Taiping Benevolent Dispensary" has been employed treating gastric discomfort, burning epigastric pain, and reflux for hundreds years shown promise ulcers (GUs). However, active components mechanism action against GUs remain unclear. The aim this study was to explore WJW elucidate underlying involved GUs. Initially, cell viability measured by counting kit 8 (CCK-8) assay evaluate efficacy WJW-containing serum vitro. ulcer index, inhibition rate, hematoxylin staining (H&E), periodic acid-Schiff (PAS) were used therapeutic effect vivo. Subsequently, levels inflammatory factors oxidative stress determined using an enzyme-linked immunosorbent assays (ELISA) on vitro vivo samples. Additionally, UPLC-Q Exactive Plus Orbitrap HRMS analyze absorbed into blood its metabolites. Network pharmacology metabolomics subsequently identify targets pathways. Real-time quantitative PCR (RT‒qPCR) Western blotting verify mRNA protein key Finally, identified molecular docking binding stability targets. ameliorated ethanol-induced damage GES-1 cells promoted healing. reduced IL-6, TNF-α, MDA, LDH while increasing IL-10, SOD, T-AOC cells. Moreover, treatment resulted decreased MDA increased PGE2, NO rats. In addition, eight blood. network results revealed 192 common entry GUs, KEGG analysis apoptosis signaling pathways main activity Metabolomic screening 13 differential There 23 components, metabolites, with TNF (TNF-α), AKT1, PTGS2 (COX2) MAPK1. significantly inhibited expression Bax, Caspase-9, Caspase-3, cleaved COX2, p-p44/42 MAPK promoting Bcl-2 p-AKT1. Molecular are berberine, palmatine, coptisine, evodiamine, rutaecarpine, evocarpine, paeoniflorin. reduces inflammation injury inhibits paper, we provide new strategy exploring formulas diseases based target mechanisms.

Language: Английский

Carbon dots with dual emission: A versatile sensing platform for rapid assay of Cr (VI) DOI
Mingyan Jia,

Lulin Peng,

Maohua Yang

et al.

Carbon, Journal Year: 2021, Volume and Issue: 182, P. 42 - 50

Published: May 27, 2021

Language: Английский

Citations

74
Silicon oxide nanoparticles alleviate chromium toxicity in wheat (Triticum aestivum L.) DOI

Natasha Manzoor,

Liaqat Ali, Temoor Ahmed

et al.

Environmental Pollution, Journal Year: 2022, Volume and Issue: 315, P. 120391 - 120391

Published: Oct. 8, 2022

Language: Английский

Citations

49
Chronic arsenic exposure induces ferroptosis via enhancing ferritinophagy in chicken livers DOI
Lu Yu,

Zhanjun Lv,

Siyu Li

et al.

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 890, P. 164172 - 164172

Published: May 16, 2023

Language: Английский

Citations

32
Prosthetic Metals: Release, Metabolism and Toxicity DOI Creative Commons
Qiang Zhong, Xin Pan, Yuhang Chen

et al.

International Journal of Nanomedicine, Journal Year: 2024, Volume and Issue: Volume 19, P. 5245 - 5267

Published: June 1, 2024

Abstract: The development of metallic joint prostheses has been ongoing for more than a century alongside advancements in hip and knee arthroplasty. Among the materials utilized, Cobalt-Chromium-Molybdenum (Co-Cr-Mo) Titanium-Aluminum-Vanadium (Ti-Al-V) alloys are predominant prosthesis construction, predominantly due to their commendable biocompatibility, mechanical strength, corrosion resistance. Nonetheless, over time, physical wear, electrochemical corrosion, inflammation induced by these that occur post-implantation can cause release various components. released metals then flow metabolize vivo, subsequently causing potential local or systemic harm. This review first details acknowledges prosthetic metals. Second, we outline concentration, biodistribution, elimination pathways Lastly, discuss possible organ, cellular, critical biomolecules, significant signaling pathway toxicities adverse effects arise from exposure Keywords: prostheses, metal release, toxicity

Language: Английский

Citations

11
Effects of hexavalent chromium on mitochondria and their implications in carcinogenesis DOI

Anish Alur,

John L. Phillips, Da-Zhong Xu

et al.

Journal of Environmental Science and Health Part C, Journal Year: 2024, Volume and Issue: 42(2), P. 109 - 125

Published: Jan. 17, 2024

Hexavalent chromium (Cr(VI)) is a well-known occupational and environmental human carcinogen. The cellular effect of Cr(VI) complex often nonspecific due to its ability modulate multiple targets. toxicity strongly linked the generation reactive oxygen species (ROS) during reduction process. ROS can cause oxidation macromolecules, such as proteins, lipids, DNA, thereby altering their functions. A major genotoxic that contributes carcinogenesis formation DNA adducts, which lead damage. Modulations signaling pathways epigenetics may also contribute carcinogenic effects Cr(VI). has impact on many aspects mitochondrial biology, including oxidative phosphorylation, mitophagy, biogenesis. These have potential alter trajectory Cr(VI)-induced This perspective article summarizes current understandings mitochondria discusses future directions research in this area, particularly with regard carcinogenesis.

Language: Английский

Citations

10
Inflammation response after the cessation of chronic arsenic exposure and post-treatment of natural astaxanthin in liver: potential role of cytokine-mediated cell–cell interactions DOI
Zhigang Zhang,

Changming Guo,

Huijie Jiang

et al.

Food & Function, Journal Year: 2020, Volume and Issue: 11(10), P. 9252 - 9262

Published: Jan. 1, 2020

Schematic diagram of the mechanism post treatment with natural astaxanthin attenuating arsenic-induced inflammatory response in rat liver.

Language: Английский

Citations

68
Silymarin alleviates docetaxel-induced central and peripheral neurotoxicity by reducing oxidative stress, inflammation and apoptosis in rats DOI
Ahmet Yardım, Sefa Küçükler, Selçuk Özdemir

et al.

Gene, Journal Year: 2020, Volume and Issue: 769, P. 145239 - 145239

Published: Oct. 15, 2020

Language: Английский

Citations

67
Pulmonary inflammatory and fibrogenic response induced by graphitized multi-walled carbon nanotube involved in cGAS-STING signaling pathway DOI
Bing Han, Xiaoqiao Wang, Pengfei Wu

et al.

Journal of Hazardous Materials, Journal Year: 2021, Volume and Issue: 417, P. 125984 - 125984

Published: May 4, 2021

Language: Английский

Citations

55
Melatonin-mediated MT2 attenuates colitis induced by dextran sodium sulfate via PI3K/AKT/Nrf2/SIRT1/RORα/NF-κB signaling pathways DOI
Ting Gao, Tie Wang, Zixu Wang

et al.

International Immunopharmacology, Journal Year: 2021, Volume and Issue: 96, P. 107779 - 107779

Published: May 24, 2021

Language: Английский

Citations

42
Deltamethrin induces apoptosis in cerebrum neurons of quail via promoting endoplasmic reticulum stress and mitochondrial dysfunction DOI
Siyu Li, Pengfei Wu, Bing Han

et al.

Environmental Toxicology, Journal Year: 2022, Volume and Issue: 37(8), P. 2033 - 2043

Published: April 21, 2022

Deltamethrin (DLM) is a widely used and highly effective insecticide. DLM exposure harmful to animal human. Quail, as bird model, has been in the field of toxicology. However, there little information available literature about quail cerebrum damage caused by DLM. Here, we investigated effect on neurons. Four groups healthy quails were assigned (10 each group), respectively given 0, 15, 30, 45 mg/kg gavage for 12 weeks. Through measurements cerebrum, it was found that induced obvious histological changes, oxidative stress, neurons apoptosis. To further explore possible molecular mechanisms, performed real-time quantitative PCR detect expression endoplasmic reticulum (ER) stress-related mRNA such glucose regulated protein 78 kD, activating transcription factor 6, inositol requiring enzyme, kinase RNA (PKR)-like ER kinase. In addition, detected ATP content evaluate functional status mitochondria. The study showed significantly increased decreased tissues. These results suggest chronic induces apoptosis via promoting stress mitochondrial dysfunction. Furthermore, our provide novel explanation DLM-induced avian

Language: Английский

Citations

38