Antibiotic resistance in soil-plant systems: A review of the source, dissemination, influence factors, and potential exposure risks

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 869, P. 161855 - 161855

Published: Jan. 25, 2023

Language: Английский

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Phthalates Promote Dissemination of Antibiotic Resistance Genes: An Overlooked Environmental Risk

Environmental Science & Technology, Journal Year: 2023, Volume and Issue: 57(17), P. 6876 - 6887

Published: April 21, 2023

Plastics–microorganism interactions have aroused growing environmental and ecological concerns. However, previous studies concentrated mainly on the direct paid little attention to ecotoxicology effects of phthalates (PAEs), a common plastic additive that is continuously released accumulates in environment. Here, we provide insights into impacts PAEs dissemination antibiotic resistance genes (ARGs) among microorganisms. Dimethyl phthalate (DMP, model PAE) at environmentally relevant concentrations (2–50 μg/L) significantly boosted plasmid-mediated conjugation transfer ARGs intrageneric, intergeneric, wastewater microbiota by up 3.82, 4.96, 4.77 times, respectively. The experimental molecular dynamics simulation results unveil strong interaction between DMP molecules phosphatidylcholine bilayer cell membrane, which lowers membrane lipid fluidity increases permeability favor ARGs. In addition, increased reactive oxygen species generation conjugation-associated gene overexpression under stress also contribute transfer. This study provides fundamental knowledge PAE–bacteria broaden our understanding risks plastics, especially niches with colonized microbes, guide control ARG spreading.

Language: Английский

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Regulation of quorum sensing for the manipulation of conjugative transfer of antibiotic resistance genes in wastewater treatment system

Water Research, Journal Year: 2024, Volume and Issue: 253, P. 121222 - 121222

Published: Jan. 29, 2024

Language: Английский

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Research progress on the origin, fate, impacts and harm of microplastics and antibiotic resistance genes in wastewater treatment plants

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: April 27, 2024

Abstract Previous studies reported microplastics (MPs), antibiotics, and antibiotic resistance genes (ARGs) in wastewater treatment plants (WWTPs). There is still a lack of research progress on the origin, fate, impact hazards MPs ARGs WWTPs. This paper fills gap this regard. In our search, we used “microplastics”, “antibiotic genes”, “wastewater plant” as topic terms Web Science, checking returned results for relevance by examining titles abstracts. study mainly explores following points: (1) origins fate MPs, antibiotics WWTPs; (2) mechanisms action sludge biochemical pools; (3) impacts WWTPs spread ARGs; (4) harm inflicted environment human body. Contaminants sewage such ARGs, antibiotic-resistant bacteria enter soil water. can travel through food chain thus reach humans, leading to increased illness, hospitalization, even mortality. will enhance understanding among they inflict

Language: Английский

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Oxytetracycline and heavy metals promote the migration of resistance genes in the intestinal microbiome by plasmid transfer

The ISME Journal, Journal Year: 2023, Volume and Issue: 17(11), P. 2003 - 2013

Published: Sept. 12, 2023

Language: Английский

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Fate and removal of fluoroquinolone antibiotics in mesocosmic wetlands: Impact on wetland performance, resistance genes and microbial communities

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 470, P. 133740 - 133740

Published: Feb. 7, 2024

Language: Английский

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Current examining methods and mathematical models of horizontal transfer of antibiotic resistance genes in the environment

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: April 4, 2024

The increasing prevalence of antibiotic resistance genes (ARGs) in the environment has garnered significant attention due to their health risk human beings. Horizontal gene transfer (HGT) is considered as an important way for ARG dissemination. There are four general routes HGT, including conjugation, transformation, transduction and vesiduction. Selection appropriate examining methods crucial comprehensively understanding characteristics mechanisms different HGT ways. Moreover, combined with results obtained from experimental methods, mathematical models could be established serve a powerful tool predicting dynamics frequencies. However, current reviews spread mainly focus on its influencing factors mechanisms, overlooking roles models. This review, therefore, delineated pathways summarized strengths limitations provided comprehensive summing-up pertaining three main ways transformation transduction. Finally, deficiencies studies were discussed, proposed future perspectives better understand assess risks dissemination through HGT.

Language: Английский

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Integrating Machine Learning with MALDI-TOF Mass Spectrometry for Rapid and Accurate Antimicrobial Resistance Detection in Clinical Pathogens

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(3), P. 1140 - 1140

Published: Jan. 28, 2025

Antimicrobial resistance (AMR) is one of the most pressing public health challenges 21st century. This study aims to evaluate efficacy mass spectral data generated by VITEK® MS instruments for predicting antibiotic in Staphylococcus aureus, Escherichia coli, and Klebsiella pneumoniae using machine learning algorithms. Additionally, potential pre-trained models was assessed through transfer analysis. A dataset comprising 2229 spectra collected, classification algorithms, including Support Vector Machines, Random Forest, Logistic Regression, CatBoost, were applied predict resistance. CatBoost demonstrated a clear advantage over other models, effectively handling complex non-linear relationships within achieving an AUROC 0.91 F1 score 0.78 E. coli. In contrast, yielded suboptimal results. These findings highlight gradient-boosting techniques enhance prediction, particularly with from less conventional platforms like MS. Furthermore, identification specific biomarkers SHAP values indicates promising clinical applications early diagnosis. Future efforts focused on standardizing refining algorithms could expand utility these approaches across diverse environments, supporting global fight against AMR.

Language: Английский

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The Overlooked Risk of Horizontal Transfer of Plasmid-Borne Antibiotic Resistance Genes Induced by Synthetic Phenolic Antioxidants

Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: 488, P. 137459 - 137459

Published: Feb. 1, 2025

Language: Английский

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Metagenomic surveys show a widespread diffusion of antibiotic resistance genes in a transect from urbanized to marine protected area

Marine Pollution Bulletin, Journal Year: 2025, Volume and Issue: 213, P. 117640 - 117640

Published: Feb. 4, 2025

Ports are hot spots of pollution; they receive pollution from land-based sources, marine traffic and port infrastructures. Marine ecosystems nearby areas can be strongly affected by port-related activities. Here, we investigated the microbiomes present in sea floor sediments along a transect harbour Livorno (Central Italy) to protected area. Results 16S rRNA amplicon sequencing metagenome assembled genomes (MAGs) analyses indicated presence different trends specific bacterial groups (e.g. phyla NB1-j, Acidobacteriota Desulfobulbales) transect, correlating with measured levels. Human pathogenic bacteria antibiotic resistance genes (ARGs) were also found. These results demonstrate pervasive impact human activities highlight importance microbiological surveillance sediments, which may constitute reservoir ARGs bacteria.

Language: Английский

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