α-Mangostin suppresses ethanol-induced gastric ulceration by regulating the Nrf2/HO-1 and NF-κB/NLRP3/caspase-1 signaling pathways and gut microbiota DOI Creative Commons

Suqin Yang,

Gang Liu,

Xiankun Xia

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(2), P. e24339 - e24339

Published: Jan. 1, 2024

α-Mangostin is a natural xanthone derivative isolated from Camellia atrophy (CA), commonly known as Lichuan black tea (LBT). The present study investigated the ameliorating effect and mechanism of α-mangostin on alcoholic gastric ulcers (GU) in rats. In vivo, relieved pathological symptoms. Moreover, regulated activation nuclear factor (erythroid-derived 2)-like 2 (Nrf2)/heme oxygenase 1 (HO-1) κB (NF-κB)/NLR family pyrin domain containing 3 (NLRP3)/caspase-1 pathways. Reactive oxygen species (ROS), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) were significantly decreased IL-10 increased, microtubule-associated protein light chain (LC3)-II/LC3-I ratio was p62 expression decreased, inducible nitric oxide synthase (iNOS) cyclooxygenase (COX-2) down-regulated. relevant mechanisms validated using GSE-1 RAW264.7 cells an vitro model. Furthermore, increased Ligilactobacillus Muribaculum abundance well propionic acid butyric contents. Therefore, possesses antioxidant anti-inflammatory properties, remodels intestinal flora dysbiosis through that may involve regulation Nrf2/HO-1 pathway NF-κB/NLRP3/caspase-1 pathway. It also increases This provides novel evidence regarding use for treating GU.

Language: Английский

Antioxidant Peptides from Monkfish Swim Bladders: Ameliorating NAFLD In Vitro by Suppressing Lipid Accumulation and Oxidative Stress via Regulating AMPK/Nrf2 Pathway DOI Creative Commons
Ming‐Feng Wu,

Qing-Hao Xi,

Sheng Yan

et al.

Marine Drugs, Journal Year: 2023, Volume and Issue: 21(6), P. 360 - 360

Published: June 16, 2023

In this study, we investigate the ameliorating functions of QDYD (MSP2), ARW (MSP8), DDGGK (MSP10), YPAGP (MSP13) and DPAGP (MSP18) from monkfish swim bladders on an FFA-induced NAFLD model HepG2 cells. The lipid-lowering mechanisms revealed that these five oligopeptides can up-regulate expression phospho-AMP-activated protein kinase (p-AMPK) proteins to inhibit sterol regulatory element binding protein-1c (SREBP-1c) increasing lipid synthesis up-regulating PPAP-α CPT-1 promoting β-oxidation fatty acids. Moreover, significantly reactive oxygen species' (ROS) production, promote activities intracellular antioxidases (superoxide dismutase, SOD; glutathione peroxidase, GSH-PX; catalase, CAT) bring down content malondialdehyde (MDA) derived peroxidation. Further investigations regulation oxidative stress was achieved through activating nuclear factor erythroid 2-related 2 (Nrf2) pathway raise levels heme oxygenase 1 (HO-1) downstream antioxidant proteases. Therefore, could serve as candidate ingredients develop functional products for treating NAFLD.

Language: Английский

Citations

77
Angiotensin-I-Converting Enzyme (ACE)-Inhibitory Peptides from the Collagens of Monkfish (Lophius litulon) Swim Bladders: Isolation, Characterization, Molecular Docking Analysis and Activity Evaluation DOI Creative Commons

Yudong Hu,

Qing-Hao Xi,

Jing Kong

et al.

Marine Drugs, Journal Year: 2023, Volume and Issue: 21(10), P. 516 - 516

Published: Sept. 28, 2023

The objective of this study was to isolate and characterize collagen angiotensin-I-converting enzyme (ACE)-inhibitory (ACEi) peptides from the swim bladders monkfish (Lophius litulon). Therefore, acid-soluble (ASC-M) pepsin-soluble (PSC-M) with yields 4.27 ± 0.22% 9.54 0.51%, respectively, were extracted using acid methods. ASC-M PSC-M contained Gly (325.2 314.9 residues/1000 residues, respectively) as major amino acid, but they had low imino content (192.5 188.6 in comparison calf skins (CSC) (216.6 residues). sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) patterns ultraviolet (UV) absorption spectrums illustrated that mainly composed type I collagen. Subsequently, three ACEi isolated a hydrolysate prepared via double-enzyme system (alcalase + neutrase) identified SEGPK (MHP6), FDGPY (MHP7) SPGPW (MHP9), molecular weights 516.5, 597.6 542.6 Da, respectively. SEGPK, displayed remarkable anti-ACE activity, IC50 values 0.63, 0.94 0.71 mg/mL, Additionally, docking assay demonstrated affinities ACE -7.3, -10.9 -9.4 kcal/mol, activity due their connection active pockets and/or sites hydrogen bonding, hydrophobic interaction electrostatic force. Moreover, could protect HUVECs by controlling levels nitric oxide (NO) endothelin-1 (ET-1). work provides an effective means for preparation collagens novel bladders, peptides, including SPGPW, serve natural functional components development health care products control hypertension.

Language: Английский

Citations

72
Chemical Characterization of Honeysuckle Polyphenols and Their Alleviating Function on Ultraviolet B-Damaged HaCaT Cells by Modulating the Nrf2/NF-κB Signaling Pathways DOI Creative Commons

Shuo-Lei Zheng,

Yumei Wang, Chang‐Feng Chi

et al.

Antioxidants, Journal Year: 2024, Volume and Issue: 13(3), P. 294 - 294

Published: Feb. 28, 2024

Scientific evidence attests that the epidermis receives excessive ultraviolet B (UVB) radiation, triggering generation of substantial quantities reactive oxygen species (ROS), which disrupted delicate equilibrium oxidation–reduction, leading to oxidative stress and inflammation. The historical use honeysuckle polyphenols (HPs) has garnered our attention due their efficacy in inhibiting damage. In this study, HPs were prepared from flowers employing an ultrasonic-assisted extraction method quantitatively analyzed by a LC–MS/MS, mechanisms underlying HPs’ antioxidative anti-inflammatory effects on UVB-irradiated HaCaT cell model systematically investigated. results showed had significant cellular repair effect cells (p < 0.001). mechanism action indicated could allow Nrf2 enter nucleus regulating dissociation Keap1, further increases activity downstream proteases (SOD CAT), ROS scavenging, reduces intracellular malondialdehyde (MDA) level. addition, down-regulate Toll-like receptor 4 (TLR4) inhibit NF-κB (P65) dissociating IκBα, resulting decrease entry into inflammatory factors (TNF-α, IL-6, IL-1β). four key compounds HPs, including chlorogenic acid, quercetin, isorhamnetin, luteolin, selected verify repairing UVB damage using molecular docking techniques. experiment suggested active effectively occupy Kelch homologue (Kelch) structural domain competitively bind with Nrf2, facilitate promotion binding, ultimately enhancing translocation nucleus. interact through hydrogen bonding, van der Waals forces, electrostatic forces its summary, can radiation be used develop health cosmetic products for treatment UV radiation-induced diseases.

Language: Английский

Citations

48
A systematic review on marine umami peptides: Biological sources, preparation methods, structure-umami relationship, mechanism of action and biological activities DOI
Yumei Wang, Zhe Zhang, Sheng Yan

et al.

Food Bioscience, Journal Year: 2024, Volume and Issue: 57, P. 103637 - 103637

Published: Jan. 17, 2024

Language: Английский

Citations

44
Novel Ca-Chelating Peptides from Protein Hydrolysate of Antarctic Krill (Euphausia superba): Preparation, Characterization, and Calcium Absorption Efficiency in Caco-2 Cell Monolayer Model DOI Creative Commons

Ming-Xue Ge,

Ruping Chen, Lun Zhang

et al.

Marine Drugs, Journal Year: 2023, Volume and Issue: 21(11), P. 579 - 579

Published: Nov. 5, 2023

Antarctic krill (Euphausia superba) is the world's largest resource of animal proteins and thought to be a high-quality for future marine healthy foods functional products. Therefore, was degreased separately hydrolyzed using flavourzyme, pepsin, papain, alcalase. Protein hydrolysate (AKH) prepared by trypsin showed highest Ca-chelating rate under optimized chelating conditions: pH 8.0, reaction time 50 min, temperature °C, material/calcium ratio 1:15. Subsequently, fourteen peptides were isolated from APK ultrafiltration series chromatographic methods identified as AK, EAR, AEA, VERG, VAS, GPK, SP, GPKG, APRGH, GVPG, LEPGP, LEKGA, FPPGR, GEPG with molecular weights 217.27, 374.40, 289.29, 459.50, 275.30, 300.36, 202.21, 357.41, 536.59, 328.37, 511.58, 516.60, 572.66, 358.35 Da, respectively. Among peptides, VERG presented ability. Ultraviolet spectrum (UV), Fourier Transform Infrared (FTIR), scanning electron microscope (SEM) analysis indicated that VERG-Ca chelate had dense granular structure because N-H, C=O -COOH groups combined Ca2+. Moreover, stable in gastrointestinal digestion can significantly improve Ca transport Caco-2 cell monolayer experiments, but phytate could reduce absorption derived chelate. protein possess potential serve supplement developing foods.

Language: Английский

Citations

43
Immunomodulatory peptides from thick-shelled mussel (Mytilus coruscus): Isolation, identification, molecular docking and immunomodulatory effects on RAW264.7 cells DOI
Zhen Wang,

Yue Fang,

Yu Zeng

et al.

Food Bioscience, Journal Year: 2024, Volume and Issue: 59, P. 103874 - 103874

Published: March 8, 2024

Language: Английский

Citations

32
Systematical Investigation on Anti-Fatigue Function and Underlying Mechanism of High Fischer Ratio Oligopeptides from Antarctic Krill on Exercise-Induced Fatigue in Mice DOI Creative Commons

Sha-Yi Mao,

Shi-Kun Suo,

Yumei Wang

et al.

Marine Drugs, Journal Year: 2024, Volume and Issue: 22(7), P. 322 - 322

Published: July 19, 2024

High Fischer ratio oligopeptides (HFOs) have a variety of biological activities, but their mechanisms action for anti-fatigue are less systematically studied at present. This study aimed to evaluate the efficacy HFOs from Antarctic krill (HFOs-AK) and explore its mechanism through establishing fatigue model endurance swimming in mice. Therefore, according comparison with group, HFOs-AK were able dose-dependently prolong time, reduce levels metabolites (lactic acid, blood urea nitrogen, ammonia), increase content glucose, muscle glycogen, liver lactate dehydrogenase creatine kinase extravasation, protect tissue damage shown enhance Na+-K+-ATPase Ca2+-Mg2+-ATPase activities ATP tissue. Meanwhile, also showed significantly antioxidant ability by increasing superoxide dismutase glutathione peroxidase decreasing level malondialdehyde. Further studies that could regulate body’s energy metabolism thus exert effects activating AMPK signaling pathway up-regulating expression p-AMPK PGC-α proteins. can be used as an auxiliary functional dietary molecules good activity applied foods.

Language: Английский

Citations

24
Identification and molecular mechanisms of novel antioxidant peptides from fermented broad bean paste: A combined in silico and in vitro study DOI
Hongbin Lin, Jianhua Zhao,

Yuqing Xie

et al.

Food Chemistry, Journal Year: 2024, Volume and Issue: 450, P. 139297 - 139297

Published: April 12, 2024

Language: Английский

Citations

22
Structural, functional and antioxidant properties of Lentinus edodes protein hydrolysates prepared by five enzymes DOI

Shu‐Jing Bing,

Xing-Shuo Chen,

Xin Zhong

et al.

Food Chemistry, Journal Year: 2023, Volume and Issue: 437, P. 137805 - 137805

Published: Oct. 19, 2023

Language: Английский

Citations

36
Integrative metabolomics and gut microbiota analyses reveal the protective effects of DHA-enriched phosphatidylserine on bisphenol A-induced intestinal damage DOI Creative Commons

Qiaoling Zhao,

Fei Yang,

Qiuyan Pu

et al.

Journal of Functional Foods, Journal Year: 2024, Volume and Issue: 117, P. 106229 - 106229

Published: May 7, 2024

The protective effects of docosahexaenoic acid-enriched phosphatidylserine (DHA-PS, 50 or 100 mg/kg) on bisphenol A (BPA)-induced intestinal damage were assessed. biochemical indices, pathological examination, non-targeted metabolomics integrated with gut microbiota analysis, and immunofluorescence analysis used to investigate the DHA-PS its underlying regulatory mechanism. treatment improved pathology tract increased expression levels Claudin-1, Occludin, ZO-1. In addition, also notably decreased interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α, antioxidant enzymes activities. Moreover, relative abundances Akkermansia, Alistipes, Butyricicoccus, Coriobacteriaceae_UCG-002, Enterorhabdus, Lachnospiraceae_UCG-006. alleviated BPA-induced by regulating kynurenine pathway tryptophan, lipid, arachidonic acid metabolisms. Overall, this study suggested that could alleviate enhancing barrier integrity, improving microbial composition metabolites, inhibiting TLR4/NF-κB pathways.

Language: Английский

Citations

12