Food & Function, Journal Year: 2024, Volume and Issue: unknown
Published: Jan. 1, 2024
Cymbopogon citratus methanolic extract shows anti-obesity effects in HCHFD rats by reducing lipids, glucose, and gene expression. Flavones bind to APJ protein, confirming its hypolipidemic activity potential for managing obesity.
Language: Английский
Marine Drugs, Journal Year: 2024, Volume and Issue: 22(7), P. 328 - 328
Published: July 22, 2024
The current research employed an animal model of 7,12-dimethylbenz(a)anthracene (DMBA)-induced mammary gland carcinogenesis. estrogen receptor-positive human breast adenocarcinoma cell line (MCF-7) was used for in vitro analysis. This combined with a network pharmacology-based approach to assess the anticancer properties Spirulina (SP) extract and understand its molecular mechanisms. results showed that administration 1 g/kg SP increased antioxidant activity by raising levels catalase (CAT) superoxide dismutase (SOD), while decreasing malonaldehyde (MDA) protein carbonyl. A histological examination revealed reduced tumor occurrence, decreased receptor expression, suppressed proliferation, promoted apoptosis protected animals. In addition, disrupted G2/M phase MCF-7 cycle, inducing reactive oxygen species (ROS) accumulation. It also enhanced intrinsic cells upregulating cytochrome c, Bax, caspase-8, caspase-9, caspase-7 proteins, downregulating Bcl-2 production. main compounds identified LC-MS/MS study were 7-hydroxycoumarin derivatives cinnamic acid, hinokinin, valeric α-linolenic acid. These substances specifically targeted three important proteins: ERK1/2 MAPK, PI3K-protein kinase B (AKT), epidermal growth factor (EGFR). Network analysis docking indicated significant binding affinity between these proteins. verified Western blot p-EGFR, p-ERK1/2, p-AKT following administration. finally reported suppress induce modulating PI3K/AKT/EGFR MAPK signaling pathways suggesting EGFR as potential target cancer (BC) treatment.
Language: Английский
Citations
8
Fitoterapia, Journal Year: 2024, Volume and Issue: 175, P. 105971 - 105971
Published: April 24, 2024
Language: Английский
Citations
7
Plants, Journal Year: 2024, Volume and Issue: 13(13), P. 1815 - 1815
Published: July 1, 2024
Rumex vesicarius L. Polygonaceae is a wildly grown plant in Egypt, North Africa, and Asia with wide traditional uses. Several studies reported its biological activities richness phytochemicals. This research addresses comprehensive metabolic profiling of the flowers, leaves, stems, roots via RP-HPLC-QTOF-MS MS/MS chemometrics. A total 60 metabolites were observed grouped into phenolic acids, flavonoids, phenols, terpenes, amino fatty organic sugars. Principal component analysis hierarchal cluster showed segregation different parts. Moreover, antioxidant capacity was determined several methods agreed previous results. Additionally, an silico approach molecular docking predominant bioactive employed against two targets, NADPH oxidase human peroxiredoxin 5 enzyme (PDB ID: 2CDU 1HD2) receptors, alongside ADME predictions. The modelling revealed that most approached molecules specifically binding tested enzymes, achieving high affinities. results confirmed R. stems are rich sources components. To our knowledge, this first giving prospect relevance development new naturally based antioxidants.
Language: Английский
Citations
5
Pharmaceuticals, Journal Year: 2025, Volume and Issue: 18(2), P. 248 - 248
Published: Feb. 12, 2025
Background: Doxorubicin (DOX) is a very powerful chemotherapy drug. However, its severe toxicity and potential for resistance development limit application. Withania somnifera L. Dunal (WIT) has therapeutic capacities, including anti-inflammatory, antioxidant, anticancer activities. This study investigates the preventative benefits of standardized WIT extract against DOX-induced renal damage in vivo. We also investigate synergistic effects combining DOX to improve efficacy breast cancer cells (MCF7-ADR). Methods: employed an animal model where rats were administered 300 mg/kg/day orally duration 14 days. Rats received injections at dose 5 mg/kg, total 15 mg, on 6th, 8th, 10th Results: Present results revealed that reduced increase levels blood urea creatinine activity kidney injury molecule-1. tissue damage, oxidative stress, pro-inflammatory markers. alleviated nuclear factor erythroid 2-related 2, heme oxygenase-1, sirtuin 1 tissues. modulated factor-κB decreased apoptotic indicators. Furthermore, improves DOX's capacity kill drug-resistant MCF7-ADR by arresting cell cycle promoting apoptosis. Chemical analysis root 34 distinct compounds, alkaloids, withanolides, flavanones, fatty acids. Conclusions: These constituents synergistically contribute WIT's anti-apoptotic properties. In addition, they confirm ability reduce systemic while improving treatment efficacy.
Language: Английский
Citations
0
ACS Omega, Journal Year: 2025, Volume and Issue: 10(9), P. 9402 - 9425
Published: Feb. 25, 2025
Marine sponges are noteworthy sources of bioactive secondary metabolites that demonstrate fundamental functions in defense mechanisms and ecological interactions. This study focused on the extraction characterization from two sponge species, Echinodictyum asperum Callyspongia siphonella, obtained Red Sea coast Saudi Arabia. Methanol generated EAE CSE extracts, which were subjected to metabolic profiling, illuminating 44 identified involving fatty acids, sterols, terpenoids, long-chain amides, alkaloids. The cytotoxic effects both assessed versus MDA-MB-231 MCF-7 breast cancer cell lines, utilizing sulforhodamine B (SRB) assay, with IC50 values about 2.7 μg/mL for 0.5 cells 8.7 45.3 cells. Flow cytometry analysis indicated extracts induced apoptosis necrosis across lines. Particularly, G2 phase arrest cells, while elevated S population As well, boosted autophagy especially Comparative analyses existing literature underscore therapeutic potential these sponge-derived medication. Molecular docking studies demonstrated robust binding affinities between selected key targets MCL-1, BCL-2, ERα. In conclusion, this research emphasizes significant features proposing their purposes treatment via distinct action.
Language: Английский
Citations
0
Food Chemistry X, Journal Year: 2024, Volume and Issue: 24, P. 101819 - 101819
Published: Sept. 7, 2024
Language: Английский
Citations
3
Inflammopharmacology, Journal Year: 2024, Volume and Issue: unknown
Published: Nov. 5, 2024
Abstract Eucalyptus essential oils (EEOs) have gained significant attention recently anticipated to their broad range of prospective benefits in various biological applications. They been proven strong antibacterial properties against a variety bacteria, fungi, and viruses. This makes them valuable combating infections supporting overall hygiene. The active compounds present these can help alleviate inflammation, making addressing inflammatory conditions such as arthritis, respiratory ailments, skin disorders. Respiratory health are another prominent aspect EEOs. Inhalation promote clear airways, relieve congestion, ease symptoms like coughs, colds, sinusitis. often utilized inhalation therapies chest rubs. be used topically or massage muscle joint pain. Furthermore, shown potential wound healing. Their antimicrobial activity helps prevent infection, while anti-inflammatory analgesic contribute reducing inflammation pain associated with wounds. In aromatherapy, EEOs renowned for invigorating uplifting qualities, promoting mental clarity, relaxation, stress relief. Overall, hold great promise applications, offering natural versatile approach well-being. Continued research exploration therapeutic will further unveil broaden applications fields.
Language: Английский
Citations
3
Food & Function, Journal Year: 2024, Volume and Issue: unknown
Published: Jan. 1, 2024
Cymbopogon citratus methanolic extract shows anti-obesity effects in HCHFD rats by reducing lipids, glucose, and gene expression. Flavones bind to APJ protein, confirming its hypolipidemic activity potential for managing obesity.
Language: Английский
Citations
2