Inhibition of calcium imbalance protects hepatocytes from vanadium exposure-induced inflammation by mediating mitochondrial-associated endoplasmic reticulum membranes in ducks

Poultry Science, Journal Year: 2023, Volume and Issue: 102(12), P. 103013 - 103013

Published: Aug. 18, 2023

Vanadium (V) is an essential mineral element in animals, but excessive V can lead to many diseases, affecting the health of humans and animals. However, molecular crosstalk between mitochondria-associated endoplasmic reticulum membranes (MAMs) inflammation under exposure still at exploratory stage. This study was conducted determine MAMs ducks. In this study, duck hepatocytes were treated with NaVO3 (0 μM, 100 200 μM) 2-aminoethyl diphenyl borate (2-APB) (IP3R inhibitor) alone or combination for 24 h. The data showed that exposure-induced cell vacuolization, enlarged intercellular space, decreased density viability. Meanwhile, hydrogen peroxide (H2O2), malonaldehyde (MDA), catalase (CAT), superoxide dismutase (SOD), reactive oxygen species (ROS) levels upregulated treatment. addition, could a marked reduction structure, destruction membrane structure overload intracellular Ca2+ mitochondrial Ca2+. Moreover, treatment resulted notable upregulation MAMs-relevant factors (IP3R, Mfn2, Grp75, MCU, VDAC1) downregulated IL-18, IL-1β, lactate dehydrogenase (LDH) supernatant. Additionally, it also significantly elevated inflammation-relevant (NLRP3, ASC, caspase-1, MAVS, TXNIP). inhibition IP3R expression attenuated V-induced variations above indicators. Collectively, our results revealed maintenance calcium homeostasis protect from injury via MAMs.

Language: Английский

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The Complex Genetic and Epigenetic Regulation of the Nrf2 Pathways: A Review

Antioxidants, Journal Year: 2023, Volume and Issue: 12(2), P. 366 - 366

Published: Feb. 3, 2023

Nrf2 is a major transcription factor that significantly regulates—directly or indirectly—more than 2000 genes. While many of these genes are involved in maintaining redox balance, others balance among metabolic pathways seemingly unrelated to oxidative stress. In the past 25 years, number factors activation, nuclear translocation, and deactivation has continued expand. The purpose this review provide an overview remarkable complexity tortuous sequence stop-and-go signals not only regulate expression repression, but may also modify transcriptional intensity as well specificity promoter recognition, allowing fluidity its gene profile depending on various structural modifications encounters journey DNA. At present, more 45 control points have been identified, which represent sites action so-called activators. pathway synergistic interplay combinations help explain potential advantages seen with phytochemical compositions simultaneously target multiple points, compared traditional pharmaceutical paradigm “one-drug, one-target”.

Language: Английский

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Di (2-ethylhexyl) phthalate and polystyrene microplastics co-exposure caused oxidative stress to activate NF-κB/NLRP3 pathway aggravated pyroptosis and inflammation in mouse kidney

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 926, P. 171817 - 171817

Published: March 19, 2024

Language: Английский

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Lycopene as a Therapeutic Agent against Aflatoxin B1-Related Toxicity: Mechanistic Insights and Future Directions

Antioxidants, Journal Year: 2024, Volume and Issue: 13(4), P. 452 - 452

Published: April 11, 2024

Aflatoxin (AFT) contamination poses a significant global public health and safety concern, prompting widespread apprehension. Of the various AFTs, aflatoxin B1 (AFB1) stands out for its pronounced toxicity association with spectrum of chronic ailments, including cardiovascular disease, neurodegenerative disorders, cancer. Lycopene, lipid-soluble natural carotenoid, has emerged as potential mitigator deleterious effects induced by AFB1 exposure, spanning cardiac injury, hepatotoxicity, nephrotoxicity, intestinal damage, reproductive impairment. This protective mechanism operates reducing oxidative stress, inflammation, lipid peroxidation, activating mitochondrial apoptotic pathway, facilitating activation biogenesis, endogenous antioxidant system, nuclear factor erythroid 2-related 2 (Nrf2)/kelch-like ECH-associated protein 1 (KEAP1) peroxisome proliferator-activated receptor-γ coactivator-1 (PGC-1) pathways, well regulating activities cytochrome P450 (CYP450) enzymes. review provides an overview lycopene against exposure-induced underlying molecular mechanisms. Furthermore, it explores profile clinical applications lycopene. The present underscores lycopene’s promising detoxification agent intent to stimulate further research practical utilization in this domain.

Language: Английский

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Heme-oxygenase-1 as a target for phthalate-induced cardiomyocytes ferroptosis

Environmental Pollution, Journal Year: 2022, Volume and Issue: 317, P. 120717 - 120717

Published: Nov. 21, 2022

Language: Английский

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Lycopene ameliorates atrazine-induced spatial learning and memory impairments by inhibiting ferroptosis in the hippocampus of mice

Food and Chemical Toxicology, Journal Year: 2023, Volume and Issue: 174, P. 113655 - 113655

Published: Feb. 13, 2023

Language: Английский

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Novel Role of Hemeoxygenase-1 in Phthalate-Induced Renal Proximal Tubule Cell Ferroptosis

Journal of Agricultural and Food Chemistry, Journal Year: 2023, Volume and Issue: 71(5), P. 2579 - 2589

Published: Jan. 25, 2023

Phthalates are widely used to improve the flexibility of poly(vinyl chloride) (PVC) polymer agriculture products. Di(2-ethylhexyl) phthalate (DEHP) is a type addition plastic and can lead many health problems. Hemeoxygenase-1 (HO-1) an extremely important molecule that releases enzymatic products promote ferroptosis. This research aimed explore function HO-1 in DEHP-induced renal proximal tubule cell In experiment, ICR male mice exposed (0, 50, 200, 500 mg/kg BW/day) DEHP for 28 days. Here, we observed induced glomeruli atrophy tubules swell. Furthermore, exposure could increase ferrous iron content decrease antioxidant activity. We also found increased expression nuclear factor-erythroid 2 p45-related factor (NFE2L2) nucleus. particular, significantly both protein mRNA levels. Glutathione peroxidase 4 (GPX4) as endogenous control ferroptosis was downregulated, which proved occurrence study, activated NFE2L2/HO-1 signaling pathway resulted tubule. connects with HO-1, providing new insights into potential roles phthalates nephrotoxicity.

Language: Английский

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The protective effects of chrysin on cadmium-induced pulmonary toxicity; a multi-biomarker approach

Environmental Science and Pollution Research, Journal Year: 2023, Volume and Issue: 30(38), P. 89479 - 89494

Published: July 15, 2023

Language: Английский

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Crotonylated BEX2 interacts with NDP52 and enhances mitophagy to modulate chemotherapeutic agent-induced apoptosis in non-small-cell lung cancer cells

Cell Death and Disease, Journal Year: 2023, Volume and Issue: 14(9)

Published: Sept. 30, 2023

Brain expressed X-linked gene 2 (BEX2) encoded protein was originally identified to promote transcription by interacting with several factors in the DNA-binding complexes. Recently, BEX2 found be localized cytosol and/or mitochondria and regulate apoptosis cancer cells tumor growth. However, molecular mechanism underlying its roles remains unclear. Here, we report that crotonylated plays an important role inhibiting chemotherapeutic agent-induced via enhancing mitophagy human lung cells. promotes facilitating interaction between NDP52 LC3B. Moreover, crotonylation at K59 is critical BEX2-mediated The K59R mutation of inhibits affecting expression elevated after anticancer drug treatment, overexpression chemotherapy-induced apoptosis. In addition, inhibition BEX2-regulated sensitizes Furthermore, growth regulating vivo. We also confirm overexpressed adenocarcinoma associated poor prognosis lymph node metastasis-free cancer. Therefore, combination treatment pharmaceutical approaches targeting BEX2-induced drugs may represent a potential strategy for NSCLC therapy.

Language: Английский

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Novel protective aspects of dietary polyphenols against pesticidal toxicity and its prospective application in rice-fish mode: A Review

Fish & Shellfish Immunology, Journal Year: 2024, Volume and Issue: 146, P. 109418 - 109418

Published: Feb. 1, 2024

Language: Английский

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