Investigation of the Impact of Saccharides on the Relative Activity of Trypsin and Catalase after Droplet and Spray Drying DOI Creative Commons

Johanna Dieplinger,

Christina Möser,

Gerhard König

et al.

Pharmaceutics, Journal Year: 2023, Volume and Issue: 15(10), P. 2504 - 2504

Published: Oct. 21, 2023

While using saccharides as stabilizers for therapeutic protein drying is common, the mechanisms underlying stabilization during remain largely unexplored. Herein, we investigated effect of different saccharides, trehalose dihydrate (TD), dextran (DEX), and hydroxypropyl β-cyclodextrins (low substitution-HP high substitution-HPB), on relative activities enzymes trypsin catalase miniaturized (MD) or spray (SD). For trypsin, presence especially HP, was beneficial, it significantly improved enzyme activity following MD. The HPB preserved trypsin's MD SD. Adding did not show a notable improvement in activities. Increasing TD beneficial SD catalase, indicated by increased activity. Molecular docking molecular dynamics simulations oftrypsin with HP revealed influence their substitution binding affinity enzyme. A higher to bind itself observed simulations. Experimentally, reduction mainly MD, attributable droplet temperature than from experiments aggregation propensity modeling helped elucidate impact size preserving drying.

Language: Английский

Gallic acid-functionalized soy protein-based multiple cross-linked hydrogel: Mechanism analysis, physicochemical properties, and digestive characteristics DOI

Shizhang Yan,

Qi Wang, Yang Li

et al.

Food Chemistry, Journal Year: 2023, Volume and Issue: 433, P. 137290 - 137290

Published: Aug. 29, 2023

Language: Английский

Citations

31
The inhibition mechanism of bound polyphenols extracted from mung bean coat dietary fiber on porcine pancreatic α-amylase: kinetic, spectroscopic, differential scanning calorimetric and molecular docking DOI
Nian X. Sun,

Jiayan Xie,

Bing Zheng

et al.

Food Chemistry, Journal Year: 2023, Volume and Issue: 436, P. 137749 - 137749

Published: Oct. 14, 2023

Language: Английский

Citations

29
Inhibition of enzymatic browning in freeze-thawed apricot fruit by combined chlorogenic acid and osmotic dehydration treatments DOI Creative Commons

Liling Yang,

Jianhu Cheng,

Kuanbo Cui

et al.

LWT, Journal Year: 2024, Volume and Issue: 198, P. 116066 - 116066

Published: April 1, 2024

In this study, the inhibitors of apricot browning were analyzed by investigating effects osmotic dehydration (OD) pretreatment combined with chlorogenic acid (CA) on cell disruption, loss phenolic substrates, and changes in key enzymes during freeze-thawing. Both OD OD-CA mitigated deterioration caused ice crystal damage significantly reduces BI apricots 43.27 59.55 compared to CK. The PPO POD activity are only 61.11% 42.13% those OD. could also protect SOD CAT activity, their being 1.41 1.14 times that group. This indicated treatment protected against activities ROS-scavenging enzymes. Compared OD, better bioactive substance apricot. contents ascorbic acid, total phenols, flavonoids 1.32 times, 1.11 1.05 higher than effectively neochlorogenic cryptochlorogenic catechins, rutin. summary, primarily reduced protecting integrity structures, minimizing potential for exposure air, enzymes, inhibiting activity.

Language: Английский

Citations

6
Improvement of emulsifying stability of coconut globulin by noncovalent interactions with coffee polyphenols DOI Creative Commons
Yile Chen, Yang Chen,

Lianzhou Jiang

et al.

Food Chemistry X, Journal Year: 2023, Volume and Issue: 20, P. 100954 - 100954

Published: Oct. 21, 2023

Coconut milk is an unstable emulsion system, mainly stabilized by proteins, which limits the development of food industry. The aim this study was to investigate mechanisms for increasing stability through interaction between coffee polyphenols (CPs) and coconut globulin (CG), main protein in milk. Caffeic acid (CA), chlorogenic (CHA), ferulic (FA) were selected as CP models. results showed that hydrogen bond interactions occurred CG CPs (CG-FA < CG-CA CG-CHA). CHA containing quinic preferentially formed a strong with CG. changed lipophilicity facilitated formation dense thick interfacial film at oil-water interface. Furthermore, CG-CPs excellent after storage, centrifugation, pH, salt treatment, especially CG-CHA. This could provide theoretical basis improving products.

Language: Английский

Citations

13
Spectroscopic Study of Glycated Β-Lactoglobulin and Theaflavin Nanocomplexes: Impact of Natural Deep Eutectic Solvent-Based Glycation on Interaction and Functional Improvement DOI

Qiulu Wang,

Aiju Chen,

Yiyun Yang

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Myricitrin, kaempferol-3-O-rutinoside, and rutin from Acacia tortilis (Forssk.) Hayne ssp. raddiana alleviate liver injury in carbon tetrachloride (CCl4)-intoxicated rats DOI

Fatima El-Ghazouani,

Oukacha Amri,

Abdellah Bouhaimi

et al.

South African Journal of Botany, Journal Year: 2025, Volume and Issue: 181, P. 172 - 180

Published: April 21, 2025

Language: Английский

Citations

0
Co-assembled whey protein and proanthocyanidins as a promising biocarrier for hydrophobic pterostilbene: Fabrication, characterization, and cellular antioxidant potential DOI Creative Commons

Weigang Zhong,

Qi Wang, Min Li

et al.

Journal of Dairy Science, Journal Year: 2023, Volume and Issue: 107(5), P. 2690 - 2705

Published: Nov. 9, 2023

The usage of food-derived polyphenols with different polarities has been limited by their instability and incompatibility. Therefore, a biocarrier was developed co-assembly whey protein isolate (WPI) hydrophilic proanthocyanidin (PC) for loading hydrophobic pterostilbene (PTE). Such superior affinity PTE than WPI alone, as determined encapsulation efficiency capacity assay, fluorescence quenching analysis, molecular docking, whereas the assembly process characterized particle size zeta potential, 3-dimensional fluorescence, scanning electron microscopy. Circular dichroism Fourier transform infrared spectroscopy spectra confirmed α-helix to β-sheet random coil transition proteins during formation nanocomplexes. Whey acted mediator through altering binding mode PC PTE, allowing them perform significant synergistic effects in enhancing 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) 2,2-diphenyl-1-picrylhydrazyl radical scavenging reducing H

Language: Английский

Citations

9
Identification of phenolic compounds from fermented Moringa oleifera Lam. leaf supplemented with Fuzhuan brick tea and their volatile composition and anti‐obesity activity DOI
Xin Li,

Haoyue Han,

Ying Ma

et al.

Journal of Food Science, Journal Year: 2024, Volume and Issue: 89(5), P. 3094 - 3109

Published: April 17, 2024

Abstract As a nutritious plant with valuable potential, the Moringa oleifera Lam. (MOL) leaf addition on Fuzhuan brick tea (FBT) for co‐fermentation (MOL‐FBT) was an industry innovation and new route to make full use of MOL leaf. After optimization extraction conditions, best conditions polyphenols method from MOL‐FBT (MFP) were 60°C 40 min (1:80, V/W) using response surface methodology. A total 30 phenolics identified quantified. Most increased after adding compared FBT polyphenols. In particular, caffeic acids found only in MFP. Moreover, MFP received high value taste, aroma, color. total, 62 volatile flavor compounds, consisting 3 acids, 5 alcohols, 15 aldehydes, 4 esters, 20 hydrocarbons, 10 ketones, others, addition, inhibited 3T3‐L1 preadipocyte differentiation dose‐dependent manner decreased lipid accumulation via peroxisome proliferator‐activated receptor gamma (PPARγ)/CCAAT/enhancer binding protein alpha (CEBPα)/cluster 36 (CD36) axis induced brown adipocyte‐like phenotype. vivo experiments further conducted confirm vitro results. regulated accumulation, glucose/insulin tolerance, improved liver kidney function, secretion pro‐inflammatory factors by PPARγ/CEBPα/CD36 alleviated inflammation fat fructose diet‐induced obese mice. summary, possesses high‐quality properties anti‐obesity effects, as well great potential be used novel functional food product.

Language: Английский

Citations

2
Discovering the antioxidant properties and interaction mechanisms of rosmarinic acid and kaempferol with grass carp hemoglobin: An experimental studies and molecular simulations perspective DOI
Hui Zhu,

Zeru Xu,

Renyu Zheng

et al.

Food Chemistry, Journal Year: 2024, Volume and Issue: 467, P. 142180 - 142180

Published: Nov. 28, 2024

Language: Английский

Citations

2
Caffeic acid, a dietary polyphenol, pre-sensitizes pancreatic ductal adenocarcinoma to chemotherapeutic drug DOI
Shruti Gupta, Harshita Tak, Khushhal Rathore

et al.

Journal of Biomolecular Structure and Dynamics, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 15

Published: Feb. 22, 2024

Resistance to chemotherapeutics is an eminent cause that leads search for options help in diminution of pancreatic ductal adenocarcinoma (PDAC) by overcoming resistance issues. Caffeic acid (CFA), a polyphenol occurring many dietary foods, known show antidiabetic and anticancer properties potential. To unveil the effect CFA on PDAC, we carried out this research PDAC cells, following which checked combination pre-sensitization effects CFA. Multitudinous web-based approaches were applied identifying targets then getting their interconnections. Subsequently, manifested in-vitro analysis showing IC50 concentrations 37.37 15.06 µM Panc-1 Mia-PaCa-2, respectively. The index with different drugs was explored showed antagonistic treatment leading further investigation pre-sensitizing effects. reduced concentration doxorubicin both cell lines also triggered ROS generation determined 2′,7′-dichlorofluorescin diacetate assay. differential gene expression after discrete genes affected i.e. N-Cad Cas9 Pi3K/AkT/mTOR along p53 Mia-PaCa-2. Collectively, study investigated role as therapeutics mechanism mitigating sensitizing chemotherapeutics.

Language: Английский

Citations

1