CHINESE JOURNAL OF ANALYTICAL CHEMISTRY (CHINESE VERSION),
Journal Year:
2024,
Volume and Issue:
52(3), P. 100376 - 100376
Published: March 1, 2024
In
this
work,
a
novel
ratiometric
electrochemical
aptasensor
was
designed
for
prostate
specific
antigen
(PSA)
detection
based
on
toehold-mediated
strand
displacement
reaction
(TMSDR).
For
PSA
detection,
could
bind
with
the
aptamer
sequence
in
hairpin
probe
1
(HP1),
and
HP1/PSA
complex
obtained.
Then,
presence
of
S3
DNA,
trigger
two
TMSDR
processes
by
hybridizing
Fc-S2/S1
double-stranded
which
would
generate
large
amount
Fc-S2
recycle
HP1/PSA.
This
enabled
hybridization
methylene
blue
labeled
thiolated
2
(MB-HP2)
immobilized
electrode
surface,
causing
MB
molecules
away
from
Fc
close
to
electrode.
Thus,
increasing
concentrations,
current
(IMB)
decreased
(IFc)
increased.
The
ratio
IFc/IMB
shown
be
linear
logarithm
concentration
pg
mL−1
100
ng
mL−1,
limit
0.28
mL−1.
addition,
had
good
specificity,
reproducibility,
repeatability,
stability
successfully
applied
spiked
diluted
human
serum
samples.
Micromachines,
Journal Year:
2023,
Volume and Issue:
14(7), P. 1412 - 1412
Published: July 13, 2023
The
rapid
and
sensitive
detection
of
food
contaminants
is
becoming
increasingly
important
for
timely
prevention
treatment
foodborne
disease.
In
this
review,
we
discuss
recent
developments
electrochemical
biosensors
as
facile,
rapid,
sensitive,
user-friendly
analytical
devices
their
applications
in
safety
analysis,
owing
to
the
characteristics
advances
design
production
bioreceptors
(antibodies,
DNA,
aptamers,
peptides,
molecular
imprinted
polymers,
enzymes,
bacteriophages,
etc.).
They
can
offer
a
low
limit
required
such
allergens,
pesticides,
antibiotic
traces,
toxins,
bacteria,
etc.
We
provide
an
overview
broad
range
biosensing
designs
consider
future
opportunities
technology
control.
Critical Reviews in Analytical Chemistry,
Journal Year:
2023,
Volume and Issue:
54(7), P. 2599 - 2617
Published: March 20, 2023
Food
safety
is
one
of
the
greatest
public
problems
occurring
around
world.
Chemical,
physical,
and
microbiological
hazards
could
lead
to
food
problems,
which
might
occur
at
all
stages
supply
chain.
To
tackle
protect
consumer
health,
specific,
accurate,
rapid
diagnosis
techniques
meeting
various
requirements
are
imperative
measures
ensure
safety.
CRISPR-Cas
system,
a
novel
emerging
technology,
effectively
repurposed
in
(bio)sensing
has
shown
tremendous
capability
develop
on-site
portable
diagnostic
methods
with
high
specificity
sensitivity.
Among
numerous
existing
CRISPR/Cas
systems,
CRISPR/Cas13a
CRISPR/Cas12a
extensively
employed
design
biosensors,
owing
their
ability
cleave
both
non-target
target
sequences.
However,
limitation
hindered
its
progress.
Nowadays,
nucleic
acid
aptamers
recognized
for
high-affinity
characteristics
analytes
incorporated
into
systems.
With
benefits
reproducibility,
durability,
portability,
facile
operation,
cost-effectiveness,
CRISPR/Cas-based
aptasensing
approaches
an
ideal
choice
fabricating
highly
specific
point-of-need
analytical
tools
enhanced
response
signals.
In
current
study,
we
explore
some
most
recent
progress
CRISPR/Cas-mediated
aptasensors
detecting
risk
factors
including
veterinary
drugs,
pesticide
residues,
pathogens,
mycotoxins,
heavy
metals,
illegal
additives,
other
contaminants.
The
nanomaterial
engineering
support
also
signified
achieve
hopeful
perspective
provide
new
straightforward
test
kits
toward
trace
amounts
different
contaminants
encountered
samples.
