Analysis of the correlation between the distribution of microorganisms carried by Coix seed and fungal toxins, and the biological control of aflatoxin

Current Research in Food Science, Journal Year: 2025, Volume and Issue: unknown, P. 101040 - 101040

Published: March 1, 2025

Coix seed is a valuable crop with both medicinal and edible uses, significantly impacting economic medical sectors. However, its contamination fungi mycotoxins poses serious health risks when used medicinally. Thus, identifying in controlling fungal toxins crucial. This study employs high-throughput sequencing to detect samples analyze their correlation mycotoxin content. Our findings showed no infection from filling maturity, but diverse community was present mature seeds, the relative abundance of dominant phyla varied across different regions. In GZQL, YNFYA, GD, Ascomycota phylum dominated, LN GXBS, Basidiomycota predominant phylum. These genera significant correlations aflatoxins, zearalenone, deoxynivalenol, indicating potential as producers. Analysis revealed that Bacillus had negative all detected mycotoxins. Antagonistic experiments demonstrated strains effectively inhibited aflatoxin production by Aspergillus flavus. provides essential data for preventing growth accumulation seed, ensuring safe use medicine food. provide developing targeted strategies prevent dietary applications. By enhancing our understanding microbial dynamics we can improve food safety public outcomes, reinforcing importance continued research this field.

Language: Английский

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Alternaria alstroemeriae F1 inhibits aspergillus flavus growth and weakens aflatoxins biosynthesis

Fungal Genetics and Biology, Journal Year: 2025, Volume and Issue: unknown, P. 103989 - 103989

Published: April 1, 2025

Language: Английский

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Establishment of a Dual-Signal Enhanced Fluorescent Aptasensor for Highly Sensitive Detection of Ochratoxin A

ACS Omega, Journal Year: 2024, Volume and Issue: 9(19), P. 21035 - 21041

Published: April 30, 2024

A robust and versatile dual-signal enhanced fluorescent aptasensor was developed for ochratoxin (OTA) detection based on fluorescence resonance energy transfer between 5-carboxyfluorescein (FAM) Super Green I (SG) fluorophores as the donor graphene oxide (GO) nanosheet acceptor. Abundant SG probes were adsorbed into FAM-complementary DNA (cDNA)-aptamer double-stranded structure to achieve remarkably responses. Without OTA, FAM-cDNA-SG conjugates coexisted with GO nanosheets, exhibiting strong signals. In presence of it captured by aptamers release cDNA-FAM probes, which GO, leading OTA-dependent quenching. The changed intensity measured accurate quantitation OTA. Under optimum conditions, realized fascinating sensitivity a limit 0.005 ng/mL wide concentration range 0.02–20 ng/mL, well high selectivity OTA over other interfering substances, excellent accuracy average recoveries 91.37–116.83% in fortified malt matrices, superior reliability practicability actual samples. This FAM-cDNA-aptamer-SG/GO nanosheet-based aptasensing platform could be extended monitor contaminants or trace molecules food, environmental, diagnostic fields altering corresponding aptamers.

Language: Английский

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Ultrasensitive Imaging Assay of Multiple Mycotoxins Using Cobalt DNA–Inorganic Hybrid Superstructure with High Chemiluminescence Catalytic Property

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(38), P. 15322 - 15329

Published: Sept. 6, 2024

A multiplex assay of mycotoxins in food and medicine is urgently needed challenging due to synergistic hazards trace a lack sensitive user-friendly detection approaches. Herein, cobalt DNA-inorganic hybrid superstructure (Co@DS) was developed through isothermal rolling circle amplification (RCA) for an ultrasensitive chemiluminescence (CL) imaging multiple mycotoxins. Cobalt ions were enriched the RCA product, endowing Co@DS with high CL catalytic property. Experimental studies elucidated formation mechanism Co@DS. facilely integrated biotinylated DNA function as universal platform combined disposable immunosensor array chip. After competitive immunoassay biotin-avidin recognition, signals luminol hydrogen peroxide, catalyzed by captured on each testing zone chip, imaged simultaneously. Target can be quantitated intensities. To validate concept, approach employed joint determination aflatoxin B

Language: Английский

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Modified Mycotoxins and Multitoxin Contamination of Food and Feed as Major Analytical Challenges

Toxins, Journal Year: 2023, Volume and Issue: 15(8), P. 511 - 511

Published: Aug. 19, 2023

Mycotoxins, as natural products of molds, are often unavoidable contaminants food and feed, to which the increasingly evident climate changes contribute a large part. The consequences more or less severe range from economic losses worrying health problems fatal outcome. One best preventive approaches is regular monitoring feed for presence mycotoxins. However, even under conditions frequent, comprehensive, conscientious controls, desired protection goal may not be achieved. In fact, it happens that, despite favorable analytical results that do indicate high mycotoxin contamination, symptoms their occur in practice. most common reasons this simultaneous several different mycotoxins whose individual content does exceed detectable prescribed values and/or alteration form mycotoxin, renders impossible analytically determined using routine methods. When such contaminated foods enter living organism, toxic effects occur. This article aims shed light on above order pay attention them, work reduce impact, and, eventually, overcome them.

Language: Английский

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High-resolution mass spectrometry-based suspect and nontarget screening of natural toxins in foodstuffs and risk assessment of dietary exposure

Environmental Pollution, Journal Year: 2024, Volume and Issue: unknown, P. 125338 - 125338

Published: Nov. 1, 2024

Language: Английский

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Mycotoxins and pyrrolizidine alkaloids in herbal dietary supplements

Food Additives and Contaminants Part B, Journal Year: 2024, Volume and Issue: 17(2), P. 180 - 192

Published: April 2, 2024

The market demand for herbal dietary supplements is rapidly growing and such products are becoming more common accessible to consumers. However, the knowledge about their safety remains incomplete. Herbal one of food groups that can contribute significantly human health concerns arising from chronic exposure pyrrolizidine alkaloids mycotoxins. This study aimed simultaneously determine 79 natural contaminants, including mycotoxins, as well tropane in analytical run. Exposure assessment risks were assessed all compounds included this study. total concentration naturally occurring contaminants reached 5.3 mg kg

Language: Английский

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Evaluation of mycotoxins, mycobiota and toxigenic fungi in the traditional medicine Radix Dipsaci

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Sept. 20, 2024

Medicinal herbs have been increasingly used for therapeutic purposes against a diverse range of human diseases worldwide. However, inevitable contaminants, including mycotoxins, in medicinal can cause serious problems humans despite their health benefits. The increasing consumption plants has made use public problem due to the lack effective surveillance use, efficacy, toxicity, and quality these natural products. Radix Dipsaci is commonly utilized traditional Chinese medicine susceptible contamination with mycotoxins. Here, we evaluated mycobiota toxigenic fungi . A total 28 out 63 sample batches (44.4%) were found contain Among positive samples, levels AFB 1 , AFG 2 OTA samples ranged from 0.52 32.13 μg/kg, 5.14 20.05 1.52 2.33 1.81 19.43 μg/kg respectively, while concentrations ZEN T-2 2.85 6.33 2.03 2.53 respectively. More than 60% contaminated combined multiple Fungal diversity community altered various abundance Aspergillus Fusarium increased aflatoxins (AFs) ZEN. 95 strains potentially isolated predominantly comprising (73.7%), (20.0%), Penicillium (6.3%). Through morphological identification, molecular mycotoxin synthase gene identification toxin production verification, confirmed that primarily derive flavus derives westerdijkiae oxysporum graminearum These data facilitate our comprehension prevalent fungal species herbal medicine, thereby aiding establishment strategies prevention, control, degradation mitigate presence mycotoxins medicine.

Language: Английский

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Simultaneous detection of multiple mycotoxins in Radix Dipsaci and estimation of exposure risk for consumers

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Oct. 1, 2024

Language: Английский

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Determination of seven mycotoxins in cereals by ultra performance liquid chromatography-quadrupole-time of flight mass spectrometry based on the self-built database

Chinese Journal of Chromatography, Journal Year: 2023, Volume and Issue: 41(11), P. 1002 - 1009

Published: Nov. 1, 2023

A method for the determination of seven mycotoxins in rice and wheat by ultra performance liquid chromatography-quadrupole-time flight mass spectrometry (UPLC-Q-TOF/MS) based on self-built database was established. Samples were extracted with 0.2% formic acid aqueous solution-acetonitrile (50∶50, v/v), dehydrated salted according to QuEChERS (4 g magnesium sulfate, 1 sodium chloride, citrate, 0.5 citrate disodium salt), separated an HSS T3 column (100 mm×2.1 mm, 1.8 μm). UPLC-Q-TOF/MS MSE screening performed, positive negative ions screened calibrated quantified using matrix-matched standard curves time multiple reaction monitoring (TOF-MRM). The results showed that aflatoxin G1 (AFG1), G2 (AFG2), B1 (AFB1), B2 (AFB2), ochratoxin (OTA) exhibited moderate matrix effects rice, while OTA zearalenone (ZEN) wheat. good linearities their respective concentration ranges, correlation coefficients (r) 0.9900-0.9998. limits detection (LODs) 0.50-400 0.50-200 μg/kg, respectively, quantification (LOQs) both cereals 1.00-800 μg/kg. In average recoveries at three spiked levels low, medium, high 88.1%-123.9%, relative deviations (RSDs) 0.2%-13.6%. wheat, 102.0%-123.4%, RSDs 0.8%-14.8%. As a result, one batch 46 batches out AFB1 AFB2, rate 2.2%, which measured values 10.8 μg/kg 1.2 respectively. According GB 2761-2017, maximum allowable level is 10 μg/kg; thus, exceeding 2.2%. Deoxynivalenol (DON) 9 24 (screening rate, 37.5%), ZEN 19 79.2%). DON are 1000 60 detected samples did not exceed these limits. proposed uses qualitative avoid occurrence false positives caused interfering compounds numbers retention times similar those analytes. TOF-MRM mode then used quantify positively mycotoxins. fast, accurate, sensitive, suitable isolation quantitative mycotoxin residues samples. findings provide powerful technical support contamination early risk-warning efforts.

Language: Английский

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