A Crucial Role of Proteolysis in the Formation of Intracellular Dinitrosyl Iron Complexes DOI Creative Commons
Karolina Wójciuk, Jarosław Sadło, Hanna Lewandowska

et al.

Molecules, Journal Year: 2024, Volume and Issue: 29(7), P. 1630 - 1630

Published: April 5, 2024

Dinitrosyl iron complexes (DNICs) stabilize nitric oxide in cells and tissues constitute an important form of its storage transportation. DNICs may comprise low-molecular-weight ligands, e.g., thiols, imidazole groups chemical compounds with low molecular weight (LMWDNICs), or high-molecular-weight peptides proteins (HMWDNICs). The aim this study was to investigate the role low- ligands DNIC formation. Lysosomal proteasomal proteolysis inhibited by specific inhibitors. Experiments were conducted on human erythroid K562 overexpressing a heavy chain ferritin. Cell cultures treated •NO donor. formation monitored electron paramagnetic resonance. Pretreatment inhibitors diminished intensity changed shape DNIC-specific EPR signal treatment time-dependent manner. level significantly influenced presence protein degradation products. Interestingly, HMWDNICs depended availability LMWDNICs. extent glutathione involvement vivo is minor yet noticeable, aligning our prior research findings.

Language: Английский

Research Progress of Nitrite Metabolism in Fermented Meat Products DOI Creative Commons

Qiyuan Shen,

Xiaoqun Zeng, Lingyu Kong

et al.

Foods, Journal Year: 2023, Volume and Issue: 12(7), P. 1485 - 1485

Published: April 1, 2023

Nitrite is a common color and flavor enhancer in fermented meat products, but its secondary amines may transfer to the carcinogen N-nitrosamines. This review focuses on sources, degradation, limitations, alteration techniques of nitrite. The transition among NO3- NO2-, NH4+, N2 constitutes balance nitrogen. Exogenous addition most source nitrite it can also be produced by contamination endogenous microbial synthesis. While degraded acids, enzymes, other metabolites lactic acid bacteria (LAB), four reductase enzymes play leading role. At deeper level, metabolism primarily regulated genes found these bacteria. By incorporating antioxidants, chromogenic agents, bacteriostats, LAB, or non-thermal plasma sterilization, amount supplied decreased, even eliminated. Finally, aim producing low-nitrite products expected achieved.

Language: Английский

Citations

28
Biological Functions of Hydrogen Sulfide in Plants DOI Open Access

Zhifeng Yang,

Xiaoyu Wang,

Jianrong Feng

et al.

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(23), P. 15107 - 15107

Published: Dec. 1, 2022

Hydrogen sulfide (H2S), which is a gasotransmitter, can be biosynthesized and participates in various physiological biochemical processes plants. H2S also positively affects plants’ adaptation to abiotic stresses. Here, we summarize the specific ways endogenously synthesized metabolized plants, along with agents methods used for research, outline progress of research on regulation plant metabolism morphogenesis, stress tolerance, series different post-translational modifications (PTMs) involved, provide reference future mechanism action.

Language: Английский

Citations

31
Induction of ferroptosis by HO-1 contributes to retinal degeneration in mice with defective clearance of all-trans-retinal DOI
Chao Chen,

Kunhuan Yang,

Danxue He

et al.

Free Radical Biology and Medicine, Journal Year: 2022, Volume and Issue: 194, P. 245 - 254

Published: Dec. 10, 2022

Language: Английский

Citations

24
Modifications in Ultrastructural Characteristics and Redox Status of Plants under Environmental Stress: A Review DOI Creative Commons
Hana Ďúranová,

Veronika Šimora,

Ľuba Ďurišová

et al.

Plants, Journal Year: 2023, Volume and Issue: 12(8), P. 1666 - 1666

Published: April 16, 2023

The rate of global environmental change is unprecedented, with climate causing an increase in the oscillation and intensification various abiotic stress factors that have negative impacts on crop production. This issue has become alarming concern, especially for countries already facing threat food insecurity. Abiotic stressors, such as drought, salinity, extreme temperatures, metal (nanoparticle) toxicities, are recognized major constraints agriculture, closely associated yield penalty losses supply. In order to combat stress, it important understand how plant organs adapt changing conditions, this can help produce more stress-resistant or stress-tolerant plants. investigation tissue ultrastructure subcellular components provide valuable insights into responses stress-related stimuli. particular, columella cells (statocytes) root cap exhibit a unique architecture easily recognizable under transmission electron microscope, making them useful experimental model ultrastructural observations. combination assessment oxidative/antioxidative status, both approaches shed light cellular molecular mechanisms involved adaptation cues. review summarizes life-threatening environment lead damage plants, emphasis their components. Additionally, selected conditions context ability survive challenging also described.

Language: Английский

Citations

15
Alleviation of arsenic toxicity in pepper plants by aminolevulinic acid and heme through modulating its sequestration and distribution within cell organelles DOI
Cengiz Kaya, Muhammad Ashraf, Mohammed Nasser Alyemeni

et al.

Environmental Pollution, Journal Year: 2023, Volume and Issue: 330, P. 121747 - 121747

Published: May 3, 2023

Language: Английский

Citations

13
Carbon monoxide/heme oxygenase system in plant: Roles in abiotic stress response and crosstalk with other signals molecules DOI Creative Commons
Feng Li, Lijuan Wei, Yayu Liu

et al.

Nitric Oxide, Journal Year: 2023, Volume and Issue: 138-139, P. 51 - 63

Published: June 24, 2023

Language: Английский

Citations

13
Self-Assembled Ferritin Nanoparticles for Delivery of Antigens and Development of Vaccines: From Structure and Property to Applications DOI Creative Commons
Shinuo Cao, Dongxue Ma, Shengwei Ji

et al.

Molecules, Journal Year: 2024, Volume and Issue: 29(17), P. 4221 - 4221

Published: Sept. 5, 2024

Ferritin, an iron storage protein, is ubiquitously distributed across diverse life forms, fulfilling crucial roles encompassing retention, conversion, orchestration of cellular metabolism, and safeguarding cells against oxidative harm. Noteworthy attributes ferritin include its innate amenability to facile modification, scalable mass production, as well exceptional stability safety. In addition, boasts unique physicochemical properties, including pH responsiveness, resilience elevated temperatures, resistance a myriad denaturing agents. Therefore, serves the substrate for creating nanomaterials typified by uniform particle dimensions biocompatibility. Comprising 24 subunits, each nanocage demonstrates self-assembly capabilities, culminating in formation nanostructures akin intricate cages. Recent years have witnessed ascendance ferritin-based self-assembled nanoparticles, owing their distinctive traits, which confer substantial advantages wide-ranging applications within biomedical domain. Ferritin highly appealing carrier delivering drug molecules antigen proteins due structural biochemical properties. This review aims highlight recent advances use novel delivery vaccine development, discussing molecular mechanisms underlying action, presenting it promising effective strategy future development.

Language: Английский

Citations

4
NO-mediated protein tyrosine nitration crosstalks with melatonin homeostasis and HO-1 activity in mitigating salt stress in sunflower seedlings DOI Creative Commons
Neha Singh,

Geetika Kalra,

Satish C. Bhatla

et al.

Journal of Plant Biochemistry and Biotechnology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 17, 2025

Language: Английский

Citations

0
Expression of the Arabidopsis Mg-chelatase H subunit alleviates iron deficiency-induced stress in transgenic rice DOI Creative Commons

Lien Hong Tran,

Jin-Gil Kim,

Sunyo Jung

et al.

Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14

Published: March 2, 2023

The most common symptom of iron (Fe) deficiency in plants is leaf chlorosis caused by impairment chlorophyll biosynthesis. Magnesium (Mg)-chelatase H subunit (CHLH) a key component both biosynthesis and plastid signaling, but its role Fe poorly understood. Heterologous expression the Arabidopsis thaliana Mg-chelatase gene ( AtCHLH ) increased activity up to 6-fold abundance product, Mg-protoporphyrin IX (Mg-Proto IX), 60–75% transgenic rice Oryza sativa seedlings compared wild-type (WT) controls. Noticeably, showed alleviation symptoms, as evidenced their less pronounced lower declines shoot growth, contents, photosynthetic efficiency, indicated F v / m electron transport rate, those WT under deficiency. Porphyrin metabolism was differentially regulated between seedlings, particularly with higher level Mg-Proto lines, showing that overexpression reprograms porphyrin rice. Leaves Fe-deficient exhibited greater upregulation deoxymugineic acid biosynthesis-related genes (i.e., NAS , NAS2 NAAT1 ), YSL2 transporter gene, Fe-related transcription factor IRO2 IDEF2 than WT, which may also partly contribute alleviating Although At CHLH postulated act receptor for abscisic (ABA), exogenous ABA did not alter phenotypes or seedlings. Our study demonstrates modulation through alleviates deficiency-induced stress, suggesting possible responses.

Language: Английский

Citations

9
Cd-induced cytotoxicity and its HO-1 and ROS quenching enzyme-mediated regulation in 2–3 leaf stage seedlings of Sorghum bicolor: An important millet crop of the arid & semi-arid regions DOI Creative Commons

Anita Singh,

Suman Parihar, G. S. Shekhawat

et al.

Journal of Trace Elements and Minerals, Journal Year: 2024, Volume and Issue: 9, P. 100165 - 100165

Published: May 19, 2024

Cadmium is a non-essential trace metal element with no known biological function. Cd toxic to both plants and human beings; hence, it of prime concern the scientific community. The objective this research find out effect heme oxygenase 1 on toxicity in Sorghum bicolor seedlings. Hydroponically adapted seedlings were treated various concentrations within range 10 200 μM. Seedlings harvested after 120 hours stress. cellular homeostasis tolerance mechanisms conducted evaluate growth parameters, stress parameters (MDA H2O2 content), non-enzymatic enzymatic (CAT, APX GPX) including HO 1. results showed that activity was measured be highest leaves at 150 μM CdCl2, which 29.61%.The correlated MDA content antioxidant enzymes concentration. revealed through decrease GPX CAT activities. Consequently, works cohort helps development plant's defense by scavenging ROS, confirmed time-dependent study. Accordingly, our highlighted might increase efficiency enhancing defence against S. bicolor.

Language: Английский

Citations

3