A homozygous mutation in the human selenocysteine tRNA gene impairs UGA recoding activity and selenoproteome regulation by selenium DOI Creative Commons
Caroline Vindry, Olivia Guillin, Philippe Wolff

et al.

Nucleic Acids Research, Journal Year: 2023, Volume and Issue: 51(14), P. 7580 - 7601

Published: May 25, 2023

Abstract The selenocysteine (Sec) tRNA (tRNA[Ser]Sec) governs Sec insertion into selenoproteins by the recoding of a UGA codon, typically used as stop codon. A homozygous point mutation (C65G) in human tRNA[Ser]Sec acceptor arm has been reported two independent groups and was associated with symptoms such thyroid dysfunction low blood selenium levels; however, extent altered selenoprotein synthesis resulting from this yet to be comprehensively investigated. In study, we CRISPR/Cas9 technology engineer heterozygous mutant cells, which then compared parental cell lines. This C65G affected many aspects integrity activity. Firstly, expression level significantly reduced due an recruitment RNA polymerase III at promoter. Secondly, strongly altered, but, more surprisingly, it no longer sensitive supplementation. Mass spectrometry analyses revealed isoform unmodified wobble nucleotide U34 cells that correlated activities. Overall, study demonstrates pleiotropic effect single on both phenotype selenoproteome expression.

Language: Английский

Expanding the Frontiers of Guardian Antioxidant Selenoproteins in Cardiovascular Pathophysiology DOI
Tommaso Angelone, Carmine Rocca, Vincenzo Lionetti

et al.

Antioxidants and Redox Signaling, Journal Year: 2024, Volume and Issue: 40(7-9), P. 369 - 432

Published: Feb. 1, 2024

Physiological levels of reactive oxygen and nitrogen species (ROS/RNS) function as fundamental messengers for many cellular developmental processes in the cardiovascular system. ROS/RNS involved cardiac redox-signaling originate from diverse sources, their are tightly controlled by key endogenous antioxidant systems that counteract accumulation. However, dysregulated redox-stress resulting inefficient removal leads to inflammation, mitochondrial dysfunction, cell death, contributing development progression disease (CVD).

Language: Английский

Citations

18
Selenium: From fluorescent probes to biomedical application DOI
Junping Wang, Mingkai Chen, Zhongyang Zhang

et al.

Coordination Chemistry Reviews, Journal Year: 2023, Volume and Issue: 493, P. 215278 - 215278

Published: June 24, 2023

Language: Английский

Citations

27
Selenium-Containing Agents Acting on Cancer—A New Hope? DOI Creative Commons
Sabrina Garbo, Silvia Di Giacomo, Dorota Łażewska

et al.

Pharmaceutics, Journal Year: 2022, Volume and Issue: 15(1), P. 104 - 104

Published: Dec. 28, 2022

Selenium-containing agents are more and considered as an innovative potential treatment option for cancer. Light is shed not only on the considerable advancements made in understanding complex biology chemistry related to selenium-containing small molecules but also Se-nanoparticles. Numerous Se-containing have been widely investigated recent years cancer therapy relation tumour development dissemination, drug delivery, multidrug resistance (MDR) immune system-related (anti)cancer effects. Despite numerous efforts, Se-agents apart from selenocysteine selenomethionine yet reached clinical trials therapy. The purpose of this review provide a concise critical overview current state art highly potent target-specific agents.

Language: Английский

Citations

31
Selenoprotein P concentrations and risk of progression from mild cognitive impairment to dementia DOI Creative Commons
Marco Vinceti, Teresa Urbano, Annalisa Chiari

et al.

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: May 31, 2023

Abstract There is a growing literature investigating the effects of selenium on central nervous system and cognitive function. However, little known about role selenoprotein P, main transporter, which can also have adverse biological effects. We conducted prospective cohort study individuals aged 42–81 years who received clinical diagnosis mild impairment. Using sandwich ELISA methods, we measured full-length P concentrations in serum cerebrospinal fluid to assess relation with dementia incidence during median follow-up 47.3 months. used Cox proportional hazards regression restricted cubic splines model such relation. Of 54 participants, 35 developed (including 26 cases Alzheimer’s dementia). Selenoprotein were highly correlated, spline analyses they each showed positive non-linear association risk, particularly after excluding diagnosed within 24 months follow-up. observed differences according subtypes considered. Risk ratios peaked at 2–6 highest levels when compared its level, depending matrix, analytical methodology subtype. Findings this study, first vivo use design evaluate associations dementia, suggest that higher circulating both fluid, predict progression MCI dementia. further confirmation these findings required, given limited statistical precision potential for residual confounding.

Language: Английский

Citations

18
Maternal selenium deficiency during pregnancy in association with autism and ADHD traits in children: The Odense Child Cohort DOI Creative Commons
Kamil Demircan, Thilo Samson Chillon, Richard Christian Jensen

et al.

Free Radical Biology and Medicine, Journal Year: 2024, Volume and Issue: 220, P. 324 - 332

Published: May 3, 2024

Selenoproteins regulate pathways controlling neurodevelopment, e.g., redox signaling and thyroid hormone metabolism. However, studies investigating maternal selenium in relation to child neurodevelopmental disorders are scarce.

Language: Английский

Citations

7
Exploring the Link between Oxidative Stress, Selenium Levels, and Obesity in Youth DOI Open Access
Teofana Bizerea, Laura Pitulice,

Otilia Bizerea-Spiridon

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(13), P. 7276 - 7276

Published: July 2, 2024

Obesity is a worldwide increasing concern. Although in adults this easily estimated with the body mass index, children, who are constantly growing and whose bodies changing, reference points to assess weight status age gender, need corroboration complementary data, making their quantification highly difficult. The present review explores interaction spectrum of oxidative stress, selenium status, obesity children adolescents. Any factor related stress that triggers and, conversely, induces part vicious circle, complex chain mechanisms derive from each other reinforce serious health consequences. Selenium its compounds exhibit key antioxidant activity also have significant role nutritional evaluation obese children. balance intake, retention, metabolism emerges as vital aspect health, reflecting interactions between diet, obesity. Understanding whether contributor or consequence could inform interventions public strategies aimed at preventing managing an early age.

Language: Английский

Citations

7
Selenium Discrepancies in Fetal Bovine Serum: Impact on Cellular Selenoprotein Expression DOI Open Access
François Parant, Fabrice Mure,

Julien Maurin

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(13), P. 7261 - 7261

Published: July 1, 2024

Selenium is an essential trace element in our diet, crucial for the composition of human selenoproteins, which include 25 genes such as glutathione peroxidases and thioredoxin reductases. The regulation selenoproteome primarily hinges on bioavailability selenium, either from dietary sources or cell culture media. This selenium-dependent control follows a specific hierarchy, with “housekeeping” selenoproteins maintaining constant expression while “stress-regulated” counterparts respond to selenium level fluctuations. study investigates variability fetal bovine serum (FBS) concentrations among commercial batches its effects stress-related cellular selenoproteins. Despite limitations study, exclusively used HEK293 cells focused subset findings highlight substantial impact levels selenoprotein expression, particularly GPX1 GPX4. luciferase reporter assay emerged sensitive precise method evaluating environments. While not exhaustive, this analysis provides valuable insights into selenium-mediated regulation, emphasizing importance responses offering guidance researchers field.

Language: Английский

Citations

6
Changes in Cerebrospinal Fluid Concentrations of Selenium Species Induced by Tofersen Administration in Subjects with Amyotrophic Lateral Sclerosis Carrying SOD1 Gene Mutations DOI Creative Commons
Marco Vinceti, Teresa Urbano, Tommaso Filippini

et al.

Biological Trace Element Research, Journal Year: 2024, Volume and Issue: unknown

Published: July 17, 2024

Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease affecting the brain and spinal cord motor neurons. On 25 April 2023, drug tofersen, an antisense oligonucleotide, received US Food Drug Administration approval for treating ALS in adults carrying mutations of SOD1 gene. We aimed at assessing whether cerebrospinal fluid concentrations selenium, element both toxicological nutritional interest possibly involved etiology progression, are modified by tofersen administration. determined selenium species anion exchange chromatography hyphenated to inductively coupled plasma-dynamic reaction cell-mass spectrometry overall using plasma sector-field mass spectrometry, baseline 6 months after active treatment ten Italian patients gene mutation. Concentrations total many substantially increased intervention, particularly inorganic (tetravalent hexavalent) organic selenomethionine compound co-eluting with selenocystine standard. Overall, these findings suggest that markedly alters status probably redox within central nervous system, due direct effect on neurons and/or blood-brain barrier. Further studies required investigate biological clinical relevance how they might relate pharmacological effects progression.

Language: Английский

Citations

6
Maternal seafood consumption is associated with improved selenium status: Implications for child health DOI Creative Commons
Nicholas V.C. Ralston,

Laura J. Raymond,

Christy L. Gilman

et al.

NeuroToxicology, Journal Year: 2024, Volume and Issue: 101, P. 26 - 35

Published: Jan. 23, 2024

Language: Английский

Citations

5
Low Levels of Selenoprotein P Are Associated With Cognitive Impairment in Patients Hospitalized for Heart Failure DOI Creative Commons
Amra Jujić, John Molvin, Erik Nilsson

et al.

Journal of Cardiac Failure, Journal Year: 2024, Volume and Issue: 30(11), P. 1452 - 1461

Published: Feb. 15, 2024

BackgroundSelenoprotein P (SELENOP) is a transporter for selenium and has been shown to protect status maintenance in brain against deficiency, supporting neuronal development, neurogenesis neurocognitive function. Selenium deficiency previously associated with cognitive impairment various populations, but no studies have carried out subjects heart failure (HF).PurposeTo explore if SELENOP acute HF impairment.MethodsPlasma measured through an immunoassay analysis well-validated marker of plasma the benefit providing information on bioavailable fraction preferentially supplied cells equipped receptors uptake. was 320 hospitalized HF. One-hundred-eighty-seven also underwent four tests assessing global function [Montreal Cognitive Assessment (MoCA)], processing [Symbol Digit Modalities Test (SDMT)], visual attention task switching [Trailmaking A (TMT-A)], executive speed [A Quick Speed (AQT) form color]. Appropriate cut-offs were used each test define impairment. Cross-sectional associations between concentrations defined by explored using multivariable logistic models. Further, models exploring as lowest quartile levels out.ResultsThe 187 participants had mean age 73 (±11.9) years; 31% female, BMI 28.1 (±5.6) kg/m2. Each 1 SD increment SELENOP-concentrations lower odds MoCA cut-off score <23 (odds ratio (OR) 0.60; 95%CI 0.40-0.91; p= 0.017). within (≤2.3 mg/L) (OR 3.10; 1.38-6.97; p=0.006), SDMT 2.26; 1.10-4.67; p=0.027) TMT-A 3.40; 1.47-7.88; p=0.004)), not AQT color.ConclusionsIn admitted failure, higher better performance reflecting cognition, SELENOP-deficiency three tests.

Language: Английский

Citations

5