Fundamentals of redox regulation in biology

Nature Reviews Molecular Cell Biology, Journal Year: 2024, Volume and Issue: 25(9), P. 701 - 719

Published: April 30, 2024

Language: Английский

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FOXO transcription factors as mediators of stress adaptation

Nature Reviews Molecular Cell Biology, Journal Year: 2023, Volume and Issue: 25(1), P. 46 - 64

Published: Sept. 14, 2023

Language: Английский

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Superoxide signalling and antioxidant processing in the plant nucleus

Journal of Experimental Botany, Journal Year: 2024, Volume and Issue: 75(15), P. 4599 - 4610

Published: March 8, 2024

The superoxide anion radical (O2·-) is a one-electron reduction product of molecular oxygen. Compared with other forms reactive oxygen species (ROS), has limited reactivity. Nevertheless, reacts nitric oxide, ascorbate, and the iron moieties [Fe-S] cluster-containing proteins. Superoxide largely been neglected as signalling molecule in plant literature favour most stable ROS form, hydrogen peroxide. However, can accumulate cells, particularly meristems, where dismutase activity ascorbate accumulation are (or absent), or when generated within lipid environment membranes. Moreover, oxidation nucleus response to environmental stresses widespread phenomenon. many intracellular compartments including mitochondria, chloroplasts, on apoplastic/cell wall face plasma membrane. nuclear production functions remain poorly documented plants. Accumulating evidence suggests that pools antioxidants such glutathione discrete separate from cytosolic pools, allowing compartment-specific nucleus. We consider potential mechanisms generation targets nucleus, together importance antioxidant processing regulating signalling.

Language: Английский

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The enzymes of the oxidative phase of the pentose phosphate pathway as targets of reactive species: consequences for NADPH production

Biochemical Society Transactions, Journal Year: 2023, Volume and Issue: 51(6), P. 2173 - 2187

Published: Nov. 16, 2023

The pentose phosphate pathway (PPP) is a key metabolic pathway. oxidative phase of this process involves three reactions catalyzed by glucose-6-phosphate dehydrogenase (G6PDH), 6-phosphogluconolactonase (6PGL) and 6-phosphogluconate (6PGDH) enzymes. first third steps (catalyzed G6PDH 6PGDH, respectively) are responsible for generating reduced nicotinamide adenine dinucleotide (NAPDH), cofactor maintaining the reducing power cells detoxification both endogenous exogenous oxidants electrophiles. Despite importance these enzymes, little attention has been paid to fact that proteins targets oxidants. In response stimuli pathways modulated, with PPP often up-regulated in order enhance or maintain reductive capacity cells. Under such circumstances, oxidation inactivation enzymes could be detrimental. Damage may result downward spiral, as depending on extent sites modification, alterations loss enzymatic activity therefore increased damage due NADPH depletion. recent years, it become evident have different susceptibilities exposure review, we discuss existing knowledge role play metabolism cells, their susceptibility special emphasis production. Perspectives achieving better understanding molecular basis within cellular environments given.

Language: Английский

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Competitive oxidation of key pentose phosphate pathway enzymes modulates the fate of intermediates and NAPDH production

Free Radical Biology and Medicine, Journal Year: 2024, Volume and Issue: 222, P. 505 - 518

Published: June 5, 2024

Language: Английский

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Oxidative stress elicits the remodeling of vimentin filaments into biomolecular condensates

Redox Biology, Journal Year: 2024, Volume and Issue: 75, P. 103282 - 103282

Published: July 24, 2024

The intermediate filament protein vimentin performs an essential role in cytoskeletal interplay and dynamics, mechanosensing cellular stress responses. In pathology, is a key player tumorigenesis, fibrosis infection. Vimentin filaments undergo distinct versatile reorganizations, behave as redox sensors. monomer possesses central α-helical rod domain flanked by N- C-terminal low complexity domains. Interactions between this type of domains play important function the formation phase-separated biomolecular condensates, which turn are critical for organization components. Here we show that several oxidants, including hydrogen peroxide diamide, elicit remodeling into small particles. Oxidative elicited diamide induces fast dissociation circular, motile dots, requires presence single cysteine residue, C328. This effect reversible, reassembly can occur within minutes oxidant removal. Diamide-elicited droplets recover fluorescence after photobleaching. Moreover, fusion cells expressing differentially tagged allows detection dots positive both tags, indicating merge upon cell fusion. aliphatic alcohol 1,6-hexanediol, known to alter interactions domains, readily dissolves diamide-elicited at concentrations, C328 dependent manner, hampers reassembly. Taken together, these results indicate oxidation promotes reversible condensate-like structures, provide primary evidence its regulated phase separation. hypothesize droplet transition could protective against irreversible damage network oxidative stress.

Language: Английский

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“Unlocking Athletic Potential: Exploring Exercise Physiology from Mechanisms to Performance”: Evidence-based sports supplements: a redox analysis

Free Radical Biology and Medicine, Journal Year: 2024, Volume and Issue: 224, P. 62 - 77

Published: Aug. 13, 2024

Language: Английский

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The Nonlinear Cysteine Redox Dynamics in the i-Space: A Proteoform-centric Theory of Redox Regulation

Redox Biology, Journal Year: 2025, Volume and Issue: 81, P. 103523 - 103523

Published: Feb. 5, 2025

The post-translational redox regulation of protein function by cysteine oxidation controls diverse biological processes, from cell division to death. However, most current site-centric paradigms fail capture the nonlinear and emergent nature in proteins with multiple cysteines. Here, we present a proteoform-centric theory grounded i-space. i-space encapsulates theoretical landscape all possible proteoforms. Using computational approaches, quantify vast size abstract i-space, revealing its scale-free architecture-elucidating disproportionate influence cysteine-rich proteins. We define mathematical rules governing proteoform dynamics. Their dynamics are inherently nonlinear, context-dependent, fundamentally constrained copy numbers. Monte Carlo simulations human PTP1B reveal extensive sampling beyond models, supporting "oxiform conjecture". This conjecture posits that highly oxidised proteoforms, molecules bearing cysteines, central regulation. In support, even 90%-reduced proteomes can house numbers unique, potentially functioanlly diverse, oxiforms. framework offers transformative lens for understanding biology

Language: Английский

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Oxidation products of proteins in plasma of newborns reflect damage inflicted by O2 supplementation and correlate with gestational age

Free Radical Biology and Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

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Versatility of vimentin assemblies: from filaments to biomolecular condensates and back

European Journal of Cell Biology, Journal Year: 2025, Volume and Issue: unknown, P. 151487 - 151487

Published: April 1, 2025

Language: Английский

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