New fungal core microbiome members of the ground nesting bee Andrena vaga: The key to oligolecty?

Basic and Applied Ecology, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

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Taxogenomic analyses of Starmerella gilliamiae f.a, sp. nov. and Starmerella monicapupoae f.a., sp. nov., two novel species isolated from plant substrates and insects

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Journal Year: 2024, Volume and Issue: 74(2)

Published: Feb. 26, 2024

Four yeast isolates collected from flowers different ecosystems in Brazil, one fruit of

Language: Английский

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The Brazilian Amazonian rainforest harbors a high diversity of yeasts associated with rotting wood, including many candidates for new yeast species

Yeast, Journal Year: 2022, Volume and Issue: 40(2), P. 84 - 101

Published: Dec. 30, 2022

Abstract This study investigated the diversity of yeast species associated with rotting wood in Brazilian Amazonian rainforests. A total 569 strains were isolated from samples collected three areas (Universidade Federal do Amazonas‐Universidade Amazonas [UFAM], Piquiá, and Carú) municipality Itacoatiara, Amazon state. The cultured nitrogen base (YNB)‐ d ‐xylose, YNB‐xylan, sugarcane bagasse corncob hemicellulosic hydrolysates (undiluted diluted 1:2 1:5). Sugiyamaella was most prevalent genus identified this work, followed by Kazachstania . frequently Schwanniomyces polymorphus , Scheffersomyces amazonensis Wickerhamomyces sp., respectively. alpha analyses showed that dryland forest UFAM diverse area, while floodplain Carú least. Additionally, difference between highest among comparisons. Thirty candidates for new obtained, representing 36% totaling 101 isolates. Among them belonging to clades Spathaspora which are recognized as genera natural xylose‐fermenting yeasts often studied biotechnological ecological purposes. results work rainforest is a tremendous source yeasts, including species.

Language: Английский

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Yeasts in the attine ant–fungus mutualism: Diversity, functional roles, and putative biotechnological applications

Yeast, Journal Year: 2021, Volume and Issue: 39(1-2), P. 25 - 39

Published: Sept. 2, 2021

Insects interact with a wide variety of yeasts, often providing suitable substrate for their growth. Some yeast-insect interactions are tractable models understanding the relationships between symbionts. Attine ants prominent insects in Neotropics and have performed an ancient fungiculture mutualistic basidiomycete fungi more than 55-65 million years. Yeasts gain access to this sophisticated mutualism, prompting diversity, ecological, biotechnological studies environment. We review half century research field, surveying recurrent yeast taxa putative ecological roles found that previous mainly covered diversity from small fraction attine ants, being Saccharomycetales, Tremellales, Trichosporonales as most frequent or yeast-like orders found. Apiotrichum, Aureobasidium, Candida, Cutaneotrichosporon, Debaryomyces, Meyerozyma, Papiliotrema, Rhodotorula, Trichomonascus, Trichosporon recovered genera. On other hand, yeasts' on ant-fungus mutualism only tapped tip iceberg. Previous established hypotheses literature cover production lignocellulosic enzymes, chemical detoxification, fungus garden protection. these parallels processes. In conclusion, ant environment has hidden potential studying biodiversity, ecology, biotechnology, which been particularly unexplored considering vast fungus-growing ants.

Language: Английский

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Lessons From Insect Fungiculture: From Microbial Ecology to Plastics Degradation

Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 13

Published: May 24, 2022

Anthropogenic activities have extensively transformed the biosphere by extracting and disposing of resources, crossing boundaries planetary threat while causing a global crisis waste overload. Despite fundamental differences regarding structure recalcitrance, lignocellulose plastic polymers share physical-chemical properties to some extent, that include carbon skeletons with similar chemical bonds, hydrophobic properties, amorphous crystalline regions. Microbial strategies for metabolizing recalcitrant been selected optimized through evolution, thus understanding natural processes modification could aid challenge dealing human-made spread worldwide. We propose look inspiration in charismatic fungal-growing insects understand multipartite degradation plant polymers. Independently evolved diverse insect lineages, fungiculture embraces passive or active fungal cultivation food, protection, structural purposes. consider there is much learn from these symbioses, special community-level biomass defensive metabolites. plant-degrading systems at core fungicultures be promising candidates degrading synthetic plastics. Here, we first compare polymers, emphasis overlapping microbial players enzymatic between processes. Second, review literature on systems, focusing features that, supporting insects' ecology also applied biotechnological Third, taking lessons communities, suggest multidisciplinary identify degraders, enzymes pathways, as well interactions interdependencies. Spanning multiomics spectroscopy, microscopy, stable isotopes probing, enrichment microcosmos, would allow systemic ecology, driving application possibilities. Detailing how metabolic landscape entangled achieve ecological success inspire sustainable efforts mitigating current environmental crisis.

Language: Английский

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Flavonoids and Phenylethylamides Are Pivotal Factors Affecting the Antimicrobial Properties of Stingless Bee Honey

Journal of Agricultural and Food Chemistry, Journal Year: 2022, Volume and Issue: 70(39), P. 12596 - 12603

Published: Sept. 26, 2022

Despite the recent approval of stingless bee honey to Argentine Food Code, there are still many gaps in information. Likely, main reason for this is that multiple ecological and chemical factors influence their production antimicrobial properties. This work combined metabolomic, microbiological, physicochemical analyses characterize ofTetragonisca fiebrigifrom Northeastern Argentina. The activity tests showed samples (n = 24) inhibited some Gram-positive Gram-negative bacteria at different sensitivity levels. Furthermore, selected high bioactivity revealed crystallizations, a positive correlation with fungal growth, presence flavonoids. major polyphenols annotated by liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis supported metabolomic tools were quercetin 3,4'-dimethyl ether, pachypodol, jaceoside, irigenin trimethyl corymboside, chrysoeriol 7-neohesperidoside, corymboside. In contrast, missing did not crystallize, lacked flavonoids, enriched phenylethylamides. Based on these findings, we discuss significance flavonoids phenylethylamides honey's food quality how they may indeed reflect essential parameters hive, such as microbial balance eubiosis.

Language: Английский

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Microbial communities associated with honey bees in Brazil and in the United States

Brazilian Journal of Microbiology, Journal Year: 2021, Volume and Issue: 52(4), P. 2097 - 2115

Published: July 15, 2021

Language: Английский

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Microbiota and pathogens in an invasive bee: Megachile sculpturalis from native and invaded regions

Insect Molecular Biology, Journal Year: 2023, Volume and Issue: 32(5), P. 544 - 557

Published: May 16, 2023

The present study aimed to characterise the bacterial, fungal and parasite gut community of invasive bee Megachile sculpturalis sampled from native (Japan) invaded (USA France) regions via 16S rRNA ITS2 amplicon sequencing PCR detection microparasites. bacterial microbiota communities in bees were highly similar differed strongly those obtained Japan. Core sequence variants (ASVs) within each population represented environmental micro-organisms commonly bee-associated niches that likely provide beneficial functions their host. Although overall M. France co-foraging Anthidium florentinum Halictus scabiosae, significantly different, five out eight core ASVs shared suggesting common sources potential transmission. None 46 analysed harboured known pathogens, while microparasite infections A. florentinum, rare H. scabiosae. A shift as a response changed conditions, or founder effect coupled re-establishment may explain observed microbial profiles absence parasites. While role pathogen pressure shaping biological invasions is still debated, natural enemies contribute invasion success sculpturalis.

Language: Английский

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Low Levels of Hive Stress Are Associated with Decreased Honey Activity and Changes to the Gut Microbiome of Resident Honey Bees

Microbiology Spectrum, Journal Year: 2023, Volume and Issue: 11(4)

Published: June 8, 2023

Honey bees (Apis mellifera) face increasing threats to their health, particularly from the degradation of floral resources and chronic pesticide exposure. The properties honey bee gut microbiome are known both affect be affected by health. Using samples healthy hives showing signs stress a single apiary with access same resources, we profiled antimicrobial activity chemical determined bacterial fungal hive environment. We found was significantly more active than stressed hives, increased phenolics antioxidant content linked higher activity. diverse in suggesting they may have less capacity exclude potential pathogens. Finally, had significant differences core opportunistically pathogenic taxa samples. Our results emphasize need for understanding proactively managing IMPORTANCE serve as pollinators many plants crops worldwide produce valuable products such wax. Various sources can disrupt colonies, affecting health productivity. Growing evidence suggests that is vitally important functioning overall In this study, stress, finding antimicrobial, content. next environment, between hives. underscore greater area, even apparently minor implications fitness well economic products.

Language: Английский

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Yeast communities associated with cacti in Brazil and the description of Kluyveromyces starmeri sp. nov. based on phylogenomic analyses

Yeast, Journal Year: 2020, Volume and Issue: 37(12), P. 625 - 637

Published: Nov. 8, 2020

Abstract Yeast communities associated with cacti were studied in three ecosystems of Southeast, Central and North Brazil. A total 473 yeast strains belonging to 72 species isolated from 190 samples collected. Cactophilic prevalent necrotic tissues, flowers, fruits insects collected Southeast Pichia cactophila , Candida sonorensis the Sporopachydermia complex most cactophilic regions. Kodamaea nitidulidarum restingae Wickerhamiella cacticola frequently cactus flowers fruits. The diversity yeasts substrates was high. Twenty‐one novel found. One is described here as Kluyveromyces starmeri sp. nov. based on 21 isolates obtained columnar Cereus saddianus Micranthocereus dolichospermaticus Pilosocereus arrabidae two different Phylogenetic analyses sequences encoding gene small subunit (SSU) rRNA gene, internal transcribed spacer, 5.8S D1/D2 domains large (LSU) showed that related dobzhanskii lactis marxianus . Phylogenomic 1264 conserved genes shared among new 19 other members Saccharomycetaceae confirmed this phylogenetic relationship. holotype K. CBS 16103 T (=UFMG‐CM‐Y3682 ). Mycobank number MB 836817.

Language: Английский

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