Frontiers in Immunology,
Journal Year:
2023,
Volume and Issue:
14
Published: Dec. 12, 2023
The
constant
appearance
of
new
severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2)
variants
concern
(VoCs)
has
jeopardized
the
protective
capacity
approved
vaccines
against
disease-19
(COVID-19).
For
this
reason,
generation
vaccine
candidates
adapted
to
emerging
VoCs
is
special
importance.
Here,
we
developed
an
optimized
COVID-19
candidate
using
modified
vaccinia
virus
Ankara
(MVA)
vector
express
a
full-length
prefusion-stabilized
SARS-CoV-2
spike
(S)
protein,
containing
3
proline
(3P)
substitutions
in
S
protein
derived
from
beta
(B.1.351)
variant,
termed
MVA-S(3Pbeta).
Preclinical
evaluation
MVA-S(3Pbeta)
head-to-head
comparison
previously
generated
MVA-S(3P)
candidate,
expressing
Wuhan
(with
also
3P
substitutions),
demonstrated
that
two
intramuscular
doses
both
fully
protected
transgenic
K18-hACE2
mice
lethal
challenge
with
reducing
mRNA
and
infectious
viral
loads
lungs
bronchoalveolar
lavages,
decreasing
lung
histopathological
lesions
levels
proinflammatory
cytokines
lungs.
Vaccination
elicited
high
titers
anti-S
Th1-biased
IgGs
neutralizing
antibodies
ancestral
strain
alpha,
beta,
gamma,
delta,
omicron.
In
addition,
similar
systemic
local
S-specific
CD4
+
CD8
T-cell
immune
responses
were
by
after
single
intranasal
immunization
C57BL/6
mice.
These
preclinical
data
support
clinical
MVA-S(3P),
explore
whether
they
can
diversify
potentially
increase
recognition
protection
VoCs.
Pathogens,
Journal Year:
2025,
Volume and Issue:
14(1), P. 23 - 23
Published: Jan. 1, 2025
The
COVID-19
pandemic
has
posed
a
significant
threat
to
global
health
systems,
with
extensive
impacts
across
many
sectors
of
society.
been
responsible
for
millions
deaths
worldwide
since
its
first
identification
in
late
2019.
Several
actions
have
taken
prevent
the
disease,
including
unprecedented
fast
development
and
vaccination
campaigns,
which
were
pivotal
reducing
symptoms
deaths.
Given
impact
pandemic,
continuous
changes
virus,
present
vaccine
technologies,
this
review
analyzes
how,
so
far,
we
met
challenge
by
emergence
new
variants
discusses
how
next-generation
pan-coronavirus
vaccines,
enhanced
longevity
breadth
immune
responses,
may
be
tackled
alternative
administration
routes
antigen
delivery
platforms.
By
addressing
these
critical
aspects,
aims
contribute
ongoing
efforts
achieve
long-term
control
COVID-19,
stimulating
discussion
work
on
vaccines
capable
facing
future
waves
infection.
Antibiotics,
Journal Year:
2023,
Volume and Issue:
12(7), P. 1220 - 1220
Published: July 22, 2023
Bacterial
infections
have
attracted
the
attention
of
researchers
in
recent
decades,
especially
due
to
special
problems
they
faced,
such
as
their
increasing
diversity
and
resistance
antibiotic
treatment.
The
emergence
development
SARS-CoV-2
infection
stimulated
even
more
research
find
new
structures
with
antimicrobial
antiviral
properties.
Among
heterocyclic
compounds
remarkable
therapeutic
properties,
benzimidazoles,
triazoles
stand
out,
possessing
antimicrobial,
antiviral,
antitumor,
anti-Alzheimer,
anti-inflammatory,
analgesic,
antidiabetic,
or
anti-ulcer
activities.
In
addition,
literature
last
decade
reports
benzimidazole-triazole
hybrids
improved
biological
properties
compared
simple
mono-heterocyclic
compounds.
This
review
aims
provide
an
update
on
synthesis
methods
these
hybrids,
along
activities,
well
structure-activity
relationship
reported
literature.
It
was
found
that
presence
certain
groups
grafted
onto
benzimidazole
and/or
triazole
nuclei
(-F,
-Cl,
-Br,
-CF3,
-NO2,
-CN,
-CHO,
-OH,
OCH3,
COOCH3),
some
heterocycles
(pyridine,
pyrimidine,
thiazole,
indole,
isoxazole,
thiadiazole,
coumarin)
increases
activity
hybrids.
Also,
oxygen
sulfur
atom
bridge
connecting
rings
generally
mentions
only
benzimidazole-1,2,3-triazole
Both
for
additional
ring
activity,
which
is
agreement
three-dimensional
binding
mode
summarizes
advances
derivatives
potential
agents
covering
articles
published
from
2000
2023.
Scientific Reports,
Journal Year:
2025,
Volume and Issue:
15(1)
Published: Feb. 9, 2025
Abstract
The
global
circulation
of
SARS-CoV-2
has
been
extensively
documented;
however,
the
dynamics
within
Central
America,
particularly
Nicaragua,
remain
underexplored.
This
study
characterizes
genomic
diversity
in
Nicaragua
from
March
2020
through
December
2022,
utilizing
1064
genomes
obtained
via
next-generation
sequencing.
These
sequences
were
selected
nationwide
and
analyzed
for
variant
classification,
lineage
predominance,
phylogenetic
diversity.
We
employed
both
Illumina
Oxford
Nanopore
Technologies
all
sequencing
procedures.
Results
indicated
a
temporal
spatial
shift
dominant
lineages,
initially
B.1
A.2
early
to
various
Omicron
subvariants
toward
study’s
end.
Significant
shifts
correlated
with
changes
COVID-19
positivity
rates,
underscoring
epidemiological
impact
dissemination.
Comparative
analysis
regional
data
underscored
low
circulating
lineages
their
delayed
introduction
compared
other
countries
American
region.
also
linked
specific
viral
mutations
hospitalization
emphasizing
clinical
relevance
surveillance.
research
advances
understanding
evolution
provides
valuable
information
regarding
its
genetic
public
health
officials
America.
highlight
critical
role
ongoing
surveillance
identifying
emergent
informing
strategies.
European Journal of Medicinal Chemistry,
Journal Year:
2023,
Volume and Issue:
260, P. 115719 - 115719
Published: Aug. 10, 2023
The
recently
discovered
coronavirus,
known
as
SARS-CoV-2,
is
a
highly
contagious
and
potentially
lethal
viral
infection
that
was
declared
pandemic
by
the
World
Health
Organization
on
March
11,
2020.
Since
beginning
of
pandemic,
an
unprecedented
number
COVID-19
vaccine
candidates
have
been
investigated
for
their
potential
to
manage
pandemic.
Herein,
we
reviewed
development
associated
research
effort,
both
traditional
forward-looking,
demonstrate
advantages
disadvantages
technology,
in
addition
efficacy
limitations
against
mutant
SARS-CoV-2.
Moreover,
report
repurposed
drug
discovery,
which
mainly
focuses
virus-based
host-based
targets,
well
inhibitors.
SARS-CoV-2
targets
include
main
protease
(Mpro),
RNA-dependent
RNA-polymerase
(RdRp),
are
most
well-studied
conserved
across
coronaviruses,
enabling
broad-spectrum
inhibitors
these
enzymes.
Cureus,
Journal Year:
2023,
Volume and Issue:
unknown
Published: Nov. 21, 2023
The
spike
protein
of
SARS-CoV-2
has
been
found
to
exhibit
pathogenic
characteristics
and
be
a
possible
cause
post-acute
sequelae
after
infection
or
COVID-19
vaccination.
vaccines
utilize
modified,
stabilized
prefusion
that
may
share
similar
toxic
effects
with
its
viral
counterpart.
aim
this
study
is
investigate
mechanisms
harm
biological
systems
from
vaccine-encoded
propose
mitigation
strategies.
We
searched
PubMed,
Google
Scholar,
‘grey
literature’
find
studies
(1)
investigated
the
on
systems,
(2)
helped
differentiate
between
vaccine-generated
proteins,
(3)
identified
detoxification
protocols
compounds
had
signals
benefit
acceptable
safety
profiles.
abundant
evidence
damage
in
cardiovascular,
hematological,
neurological,
respiratory,
gastrointestinal,
immunological
systems.
Viral
proteins
have
shown
play
direct
role
cardiovascular
thrombotic
injuries
both
Detection
for
at
least
6-15
months
vaccination
those
indicates
as
primary
contributing
factor
long
COVID.
rationalized
these
findings
give
support
potential
long-term
post-infection
and/or
vaccine-induced
complications.
base
protocol,
composed
oral
nattokinase,
bromelain,
curcumin.
This
approach
holds
immense
promise
clinical
care,
upon
which
additional
therapeutic
agents
are
applied
goal
aiding
resolution
Large-scale,
prospective,
randomized,
double-blind,
placebo-controlled
trials
warranted
order
determine
relative
risks
benefits
protocol.
Preparative Biochemistry & Biotechnology,
Journal Year:
2025,
Volume and Issue:
unknown, P. 1 - 10
Published: Jan. 31, 2025
Although
COVID-19
is
now
becoming
endemic,
SARS-CoV-2
persists
potential
jeopardy
to
clinically
vulnerable
populations.
Hence,
further
study
still
necessary
discover
novel
antiviral
agents
against
for
proactive
preparedness.
papain-like
protease
(PL
Pro)
a
target
enzyme
searching
anti-Covid
candidates.
Our
prior
revealed
the
major
formation
of
inclusion
bodies
during
PL
Pro
expression
in
E.
coli
RIPL.
In
this
study,
we
tried
using
chaperonin
Arctic
Express
system
and
both
codon
optimization
maltose-binding
protein
(MBP)
fusion
make
more
soluble.
Recombinant
encoded
on
pET21d(+)
plasmid
was
expressed
express.
However,
soluble
yield
remained
low
unstable
due
suboptimal
usage
insert
gene.
Whereas,
MBP
with
optimized
enhanced
solubility.
cleaved
linker
between
Pro,
which
served
as
cleavage
site
recognized
by
(LKGG↓A).
The
purified
from
200-mL
culture
generated
1
mL
pure
at
1.913
mg/mL
concentration.
It
exhibited
favorable
activity
Z-RLRGG-AMC
substrate,
Km
value
33.40
μM
Vmax
5.10
RFU/min.
European Radiology,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 19, 2025
Abstract
Purpose
To
assess
the
relationship
between
COVID-19
pandemic
and
spectrum
of
indications
for
cardiac
magnetic
resonance
imaging
(MRI)
with
a
focus
on
myocarditis.
Materials
methods
This
was
retrospective
analysis
data
from
MRCT
registry
European
Society
Cardiovascular
Radiology
(ESCR).
Data
regarding
diagnoses
myocarditis
January
1,
2018,
April
30,
2024,
were
extracted.
analyzed
periods
before
after
beginning
(i.e.,
March
11,
2020)
pandemic.
Results
112,361
MRI
examinations
(63.5%
male,
median
age
58
[IQR
44–69]).
Over
entire
period,
assessment
most
common
indication
(31%,
n
=
34,906/112,361).
Before
pandemic,
this
comprised
28%
increased
to
maximum
41%
in
2022
onset
Simultaneously,
positivity
rate
these
decreased
21%
14%
2022.
Male
patients
had
higher
than
female
both
during
mirroring
trends
sexes.
The
proportion
performed
suspected
known
coronary
artery
disease
showed
an
inverse
those
24%
17%
pre-pandemic
minimum
13%
Conclusion
considerably
influenced
pattern
referrals
Europe,
leading
but
reduced
rate,
suggesting
lower
referral
threshold
indication.
At
same
time,
proportionally
fewer
disease.
Key
Points
Question
may
have
rates
MRI,
especially
.
Findings
led
Clinical
relevance
proportions
give
important
information
historical
current
provide
insight
into
resource
deployment
