Extraction, purification, and functionality of proteins using deep eutectic solvents: a review DOI

Ali Khanalipour,

Mahnaz Tabibiazar, Behzad Masoumi

et al.

Journal of Molecular Liquids, Journal Year: 2025, Volume and Issue: unknown, P. 127623 - 127623

Published: April 1, 2025

Language: Английский

A review on the emerging conversion technology of cellulose, starch, lignin, protein and other organics from vegetable-fruit-based waste DOI
Zhongchuang Liu

International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 242, P. 124804 - 124804

Published: May 13, 2023

Language: Английский

Citations

18
Promoting the disassemble and enzymatic saccharification of bamboo shoot shells via efficient hydrated alkaline deep eutectic solvent pretreatment DOI
Qiang Hu, Ying Xu,

Yanyun Wang

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 264, P. 130702 - 130702

Published: March 11, 2024

Language: Английский

Citations

7
Solvent Tolerance Improvement of Lipases Enhanced Their Applications: State of the Art DOI Creative Commons

Mei Chen,

Tongtong Jin,

Binbin Nian

et al.

Molecules, Journal Year: 2024, Volume and Issue: 29(11), P. 2444 - 2444

Published: May 22, 2024

Lipases, crucial catalysts in biochemical synthesis, find extensive applications across industries such as food, medicine, and cosmetics. The efficiency of lipase-catalyzed reactions is significantly influenced by the choice solvents. Polar organic solvents often result a decrease, or even loss, lipase activity. Conversely, nonpolar induce excessive rigidity lipases, thereby affecting their While advent new like ionic liquids deep eutectic has somewhat improved activity stability it fails to address fundamental issue lipases' poor solvent tolerance. Hence, rational design lipases for enhanced tolerance can boost industrial performance. This review provides comprehensive summary structural characteristics properties various systems emphasizes strategies protein engineering non-aqueous media improve study theoretical foundation further enhancing lipases.

Language: Английский

Citations

7
Assessing the compatibility of choline-based deep eutectic solvents for the structural stability and activity of cellulase: Enzyme sustain at high temperature DOI
Niketa Yadav, Deepak Chahar, Meena Bisht

et al.

International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 249, P. 125988 - 125988

Published: July 25, 2023

Language: Английский

Citations

14
Natural deep eutectic solvents (NaDES): translating cell biology to processing DOI Creative Commons

Miša Mojca Cajnko,

Filipa A. Vicente, Uroš Novak

et al.

Green Chemistry, Journal Year: 2023, Volume and Issue: 25(22), P. 9045 - 9062

Published: Jan. 1, 2023

This review examines the possible functional roles of liquid natural deep eutectic solvents (NaDES) in plants and translating it to laboratory.

Language: Английский

Citations

14
Preparation and investigation of green and cheap deep eutectic solvents for determination of essential and toxic metal ions at trace levels in different coffee products DOI
Adıl Elık,

Nail Altunay

Sustainable Chemistry and Pharmacy, Journal Year: 2024, Volume and Issue: 39, P. 101602 - 101602

Published: May 8, 2024

Language: Английский

Citations

6
Natural deep eutectic solvent-ultrasound for the extraction of flavonoids from Fructus aurantii: Theoretical screening, experimental and mechanism DOI Creative Commons

Hua Du,

Wenhui Wu,

Yaodeng Wang

et al.

Arabian Journal of Chemistry, Journal Year: 2024, Volume and Issue: 17(9), P. 105886 - 105886

Published: June 21, 2024

To reduce the environmental pollution caused by organic solvents and improve extraction efficiency, a green high-effective natural deep eutectic solvent (NADES) was screened out prepared for of flavonoids from Fructus aurantii based on COMOS-RS experimental verification. The results showed theoretical screening verification were consistent, Ch-Lea best solvent, which further proved an efficient tool screening. Then, conditions investigated. optimum as follows: molar ratio (1:2), water content (30%), solid–liquid (1/60 g/g) time (15 min), yet temperature power did not significantly affect yield. Under optimal condition, yield 122.68 mg/g. Compared with traditional (methanol ethanol), has higher And assessment conducted Eco-Scale methodology, indicated more environmentally friendly than methanol ethanol. Meanwhile, good reusability, yields four reuses mg/g, 125.13 122.56 mg/g 105.63 respectively. Moreover, mechanism delved into using molecular dynamics (MD) simulation reduced density gradient (RDG) analysis. hydrogen bond interaction, particularly O-H bonds, between naringin molecule emerged primary force driving process. In summary, is green, economical, highly effective making it promising candidate flavonoid aurantii. Its potential alternative to underscores its value.

Language: Английский

Citations

5
Stabilization of Eversa® Transform 2.0 lipase with sorbitol to enhance the efficiency of ultrasound-assisted biodiesel production DOI Creative Commons
Min Ying Liow, Eng‐Seng Chan, Wei Zhe Ng

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 276, P. 133817 - 133817

Published: July 11, 2024

Ultrasound technology has emerged as a promising tool for enhancing enzymatic biodiesel production, yet the cavitation effect induced can compromise enzyme stability. This study explored efficiency of polyols in lipase stability under ultrasound conditions to further improve yield. The incorporation sorbitol resulted highest fatty acid methyl ester (FAME) content ultrasound-assisted production catalyzed by Eversa® Transform 2.0 among investigated polyols. Furthermore, enhanced lipase, allowing it tolerate up 100 % amplitude, compared 60 amplitude its absence. Enzyme activity assays revealed that preserved 99 activity, contrast 84 retention observed without an 80 amplitude. Circular dichroism (CD) and fluorescence spectroscopy analyses confirmed rigidity conformational structure exposure. employing stepwise methanol addition strategy reactions with achieved 81.2 wt% FAME 8 h only 0.2 concentration. result highlights potential stabilizing agent offering viable approach yield industrial applications.

Language: Английский

Citations

5
Optimisation and modelling of ultrasonic-assisted extraction of canthaxanthin from Chromochloris zofingiensis using eutectic solvents DOI

Dan Yang,

Weijia Qiu,

Ying Xu

et al.

Industrial Crops and Products, Journal Year: 2023, Volume and Issue: 202, P. 117002 - 117002

Published: June 15, 2023

Language: Английский

Citations

13
In Situ Encapsulation of Cytochrome c within Covalent Organic Frames Using Deep Eutectic Solvents under Ambient Conditions DOI

Liangwei Li,

Xiaoling Wu, Yuxia Pang

et al.

ACS Applied Materials & Interfaces, Journal Year: 2023, Volume and Issue: 15(46), P. 53871 - 53880

Published: Nov. 9, 2023

In situ integration of enzymes with covalent organic frameworks (COFs) to form hybrid biocatalysts is both significant and challenging. this study, we present an innovative strategy employing deep eutectic solvents (DESs) synergistically synthesize COFs shield cytochrome c (Cyt c). By utilizing DESs as reaction in combination water, successfully achieved rapid encapsulation Cyt within (specifically COF-TAPT-TFB) under ambient conditions. The resulting c@COF-TAPT-TFB composite demonstrates a remarkable preservation enzymatic activity. This also imparts exceptional resistance exhibits impressive recycling stability. Additionally, the enhanced catalytic efficiency photoenzymatic cascade showcased.

Language: Английский

Citations

13