Efficient and feasible biocatalysts: Strategies for enzyme improvement. A review

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 276, P. 133978 - 133978

Published: July 20, 2024

Language: Английский

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Process strategies for recovery of sugars, lipids, and lignin from oilcane bagasse using natural deep eutectic solvents (NADES)

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 493, P. 152657 - 152657

Published: May 29, 2024

Language: Английский

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Harnessing solvation-guided engineering to enhance deep eutectic solvent resistance and thermostability in enzymes

Green Chemistry, Journal Year: 2024, Volume and Issue: 26(16), P. 9132 - 9141

Published: Jan. 1, 2024

A rational design strategy named solvation-guided engineering was developed to modify enzyme resistance DESs and high temperatures.

Language: Английский

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Understanding the intricacy of protein in hydrated deep eutectic solvent: Solvation dynamics, conformational fluctuation dynamics, and stability

International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 253, P. 127100 - 127100

Published: Sept. 29, 2023

Language: Английский

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Enhancing biomass enzymatic hydrolysis performance by modified DES lignin

Journal of Biotechnology, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

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Optimization of Lipase Activity in Aspergillus niger C2J6 Whole Cells Using Choline Chloride Ethylene Glycol Deep Eutectic Solvent

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 30, 2025

Deep eutectic solvents (DES), recognized as tunable green solvents, show significant potential for enhancing enzyme activity in biocatalytic applications. This study investigated the effect of choline chloride–ethylene glycol DES on lipase Aspergillus niger C2J6 whole cells, employing a self-isolated endophytic strain. By varying molar ratio (1:2–1:4) and water content (0–80%), highest (142.31%) was observed at 1:1.55 with 46% content. Mathematical models were developed to connect composition key properties, including surface tension, conductivity, density, viscosity, refractive index, polarity, activity. Statistical analysis revealed that among physicochemical properties DES, polarity exhibited most impact enzymatic activity, followed by conductivity. provides valuable insights designing optimized systems improve efficiency precision.

Language: Английский

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Eutectogels as Promising Materials in Biocatalysis

ChemCatChem, Journal Year: 2024, Volume and Issue: 16(12)

Published: May 3, 2024

Abstract The entrapment of deep eutectic solvents (DES) and systems into porous scaffolds renders a new class soft nonvolatile materials called eutectogels that have recently stepped the spotlight in different areas ranging from electronics to drug delivery. Recent progress use DES biocatalysis, where they been demonstrated improve substrate supply, conversion, enzyme stability, has opened an unparalleled opportunity exploit merits for immobilizing biological catalysts. resulting functional could outperform traditional hydrogels ionic liquid gels, offering fresh perspectives broaden application scope many enzymes. In this perspective, we go potential as innovative support biocatalytic reactions discuss applications these show plain benefits compared materials. Future directions newly developed technology are highlighted.

Language: Английский

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Impact of Choline Hydroxide-Supported Magnetic Nanoparticles on Peroxidase Activity and Conformational Stability of Cytochrome c

ACS Applied Bio Materials, Journal Year: 2024, Volume and Issue: 7(2), P. 1135 - 1145

Published: Jan. 23, 2024

Nanotechnology has advanced significantly; however, little is known about the potential implications on human health-related issues, particularly blood carrying enzymes. Ionic liquids are also well-recognized for maintaining structure and activity of In this regard, we delineate a facile synthetic approach preparation Fe3O4 nanoparticles (NPs) as well choline hydroxide [CH][OH] ionic liquid (IL)-supported NPs (Fe3O4–CHOH). This combining magnetic (MNPs) with IL results in distinctive properties, which may offer enormous utility field biomedical research due to effortless separation MNPs by an external field. Detailed characterization including Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Raman spectroscopy, transmission electron microscopy (TEM), scanning (SEM) was carried out. The biomolecular interactions Fe3O4–CHOH cytochrome c (Cyt c) were studied detail using various spectroscopic microscopic techniques. From studies, it can be concluded that secondary Cyt more stable presence than NPs. binding constant calculated Benesi–Hildebrand equation. Furthermore, dynamic light scattering (DLS), ζ-potential, studies performed study interaction MNPs. These provided evidence favoring formation bionanoconjugates Moreover, enzymatic increases both peroxidase explicitly elucidates enzyme preserved long time Later on, TEM emission (FESEM) gather information regarding morphology

Language: Английский

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Cellulolytic potential of mangrove bacteria Bacillus haynesii DS7010 and the effect of anthropogenic and environmental stressors on bacterial survivability and cellulose metabolism

Environmental Research, Journal Year: 2024, Volume and Issue: 252, P. 118774 - 118774

Published: March 27, 2024

Language: Английский

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Effect of deep eutectic solvents on activity, stability, and selectivity of enzymes: Novel insights and further prospects

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: unknown, P. 138148 - 138148

Published: Nov. 1, 2024

Language: Английский

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