Identification of Bioactive Compounds by GC-MS of Nelumbo Nucifera Leaf Extract and Virtual Screening of EGFR/ VEGFR2 Dual Inhibitors DOI Open Access
Veerabhuvaneshwari Veerichetty, Iswaryalakshmi Saravanabavan,

A B Pradeep

et al.

Biosciences Biotechnology Research Asia, Journal Year: 2024, Volume and Issue: 21(3), P. 821 - 837

Published: Sept. 30, 2024

Background: Epidermal growth factor receptor (EGFR) and vascular endothelial 2 (VEGFR2) play a pivotal role in cancer progression melanoma resistance. Several pharmacophore screening studies have been done on dual tyrosine kinases inhibition of EGFR VEGFR2 for anticancer application. Objective: This study seeks to conduct virtual bioactive compounds derived from Nelumbo nucifera leaf extract, with the goal identifying potential capable against VEGFR2. Methods: Extracted nucifera, were identified through GC-MS-MS spectroscopy. In silico molecular docking was carried out using AutoDock Vina, structures visualized PyMol Biovia Discovery Studio software. The validation conducted DINC included reference drug standards. Results: Fifteen anti-carcinogenic molecules explored via GC-MS analysis Nucifera extract showed EGFR/VEGFR cutoff energy activity greater than -5kcal/mol. Docking is validated RMSD values computed. results represent has promising inhibitor Hence, 70% Bioactive various medicinal properties like antioxidant, anti-inflammatory antitumor. Thus, these 15 can overcome chemoresistance observed by BRAF inhibitors will be proved further vitro studies. Conclusion: conclusion, that present as therapeutic potential. combined suppresses tumor angiogenesis. activation overexpression causes resistance Inhibitors vemurafenib promotes angiogenesis metastasis melanoma, hence suppresion an effective anti-cancer therapy.

Language: Английский

Recent advances and applications of novel advanced materials in solid-phase microextraction for natural products DOI

Huang-Fei Jin,

Ying Shi,

Jun Cao

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: 178, P. 117858 - 117858

Published: July 6, 2024

Language: Английский

Citations

18
LC/MS/MS and GC/MS/MS metabolic profiling of Leontodon hispidulus, in vitro and in silico anticancer activity evaluation targeting hexokinase 2 enzyme DOI Creative Commons
Noha Mokhtar Abd-El-Aziz,

Mohamed Saeed Hifnawy,

Rehab Lotfy

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: March 22, 2024

Abstract Leontodon hispidulus Boiss is a wild annual plant growing in Egypt. The present study aims for the first time, to evaluate phytochemical profile of main secondary metabolites optimized ethanolic extract using Quadrupole Time-of-Flight Liquid chromatography-mass spectrometry and Gas spectrometry. It also assess anticancer activity its different fractions against prostate carcinoma cell line. Moreover, an in-silico docking was performed Hexokinase-two enzyme. LC- q ToF-MS analysis revealed tentative identification 36 phenolic compounds including glycosides (luteolin, quercetin, kaempferol, apigenin, isorhamnetin, daidzein), coumarines (esculin, esculetin, daphnetin), acids (chlorogenic, caffeic, quinic, P -coumaric, rosmarinic). GC–MS/MS presence 18 where palmitic acid, myristic alpha-amyrin, beta-amyrin were major ones. cytotoxic results that methylene chloride ethyl acetate showed highest PC3 line, with IC 50 values 19, 19.6 μg/ml, respectively. Interestingly, demonstrated apigenin-7- O -glucoside, luteolin-7- kaempferol-3- -glucuronide, quercetin-4′- esculin, rosmarinic chlorogenic α-amyrin exhibited high affinity selected target, HEK-2

Language: Английский

Citations

12
Anti-Aging Effects of Flavonoids from Plant Extracts DOI Creative Commons
Bogdan Păcularu‐Burada,

Alexandru-Ionuț Cîrîc,

Mihaela Begea

et al.

Foods, Journal Year: 2024, Volume and Issue: 13(15), P. 2441 - 2441

Published: Aug. 2, 2024

Aging is a natural and irreversible process, affecting living organisms by negatively impacting the tissues' cells' morphology functionality consequently being responsible for aging-related diseases. Taking into account actual preoccupations of both consumers researchers, healthy anti-aging alternatives are intensively studied in order to address such concerns. Due their functional features, plant flavonoids can be considered valuable nutraceuticals. This paper highlights possibilities use extracted from various plants potential on skin, brain, heart. Moreover, anticarcinogenic, anti-inflammatory, anti-diabetic properties summarized, along with senescence-associated mechanisms. Both nutraceutical cosmeceutical fields continuously developing originating promising candidates obtain products. Thus, bioactive compounds' extraction subsequent involvement innovative product manufacturing must carefully performed while aware intrinsic extrinsic factors that may affect phytochemicals' structures, bioavailability, health effects.

Language: Английский

Citations

12
Lotus (Nelumbo nucifera): a multidisciplinary review of its cultural, ecological, and nutraceutical significance DOI Creative Commons
Hang Yang, Simai He,

Qi Feng

et al.

Bioresources and Bioprocessing, Journal Year: 2024, Volume and Issue: 11(1)

Published: Jan. 27, 2024

Abstract This comprehensive review systematically examines the multifarious aspects of Nelumbo nucifera , elucidating its ecological, nutritional, medicinal, and biomimetic significance. Renowned both culturally scientifically, manifests remarkable adaptability, characterized by extensive distribution across varied climatic regions, underpinned robust rhizome system prolific reproductive strategies. Ecologically, this species plays a crucial role in aquatic ecosystems, primarily through biofiltration, thereby enhancing habitat biodiversity. The rhizomes seeds are nutritionally significant, being rich sources dietary fiber, essential vitamins, minerals, have found culinary applications. From medicinal perspective, diverse constituents exhibit therapeutic potential, including anti-inflammatory, antioxidant, anti-cancer properties. Recent advancements preservation technology innovation further underscored food industry, highlighting nutritional versatility. In biomimetics, unique "lotus effect" is leveraged for development self-cleaning materials. Additionally, transformation into biochar explored potential sustainable environmental practices. emphasizes critical need targeted conservation strategies to protect against threats posed climate change loss, advocating utilization as significant value. Graphical

Language: Английский

Citations

7
Comparison of Balanites aegyptiaca parts: metabolome providing insights into plant health benefits and valorization purposes as analyzed using multiplex GC-MS, LC-MS, NMR-based metabolomics, and molecular networking DOI Creative Commons
Mohamed A. Farag, Mostafa H. Baky, Ibrahim Morgan

et al.

RSC Advances, Journal Year: 2023, Volume and Issue: 13(31), P. 21471 - 21493

Published: Jan. 1, 2023

Balanites aegyptiaca (L.) Delile (Zygophyllaceae), also known as the desert date, is an edible fruit-producing tree popular for its nutritional and several health benefits.

Language: Английский

Citations

14
Skin anti-aging potential of Launaea procumbens extract: Antioxidant and enzyme inhibition activities supported by ADMET and molecular docking studies DOI Creative Commons
Hanan Khojah, Shaimaa R. Ahmed,

Shahad Y. Alharbi

et al.

Saudi Pharmaceutical Journal, Journal Year: 2024, Volume and Issue: 32(7), P. 102107 - 102107

Published: May 25, 2024

Aging is a natural process that occurs in all living organisms. Particularly, the skin embodies aging since it serves as barrier between body and its surroundings. Previously, we reported wound healing effect of Launaea procumbens identified compounds therein. The study aims to explore anti-aging properties plant extract. To effect, antioxidant potential L. methanolic extract (LPM) was assessed using two complementary DPPH FRAP assays. enzyme inhibitory on collagenase, elastase, hyaluronidase, tyrosinase evaluated assess direct effects. Similarly, anti-inflammatory activity indirect effects via assessment cyclooxygenase-2 (COX-2) 5-lipoxygenase (5-LOX). In addition, ADMET molecular docking studies were performed interaction mechanisms LPM with target enzymes. demonstrated significant (IC50 = 29.08 µg/mL) (1214.67 µM FeSO4/g extract) Plant showed inhibition 52.68, 43.76, 31.031, 37.13 µg/mL, respectively). COX-2 5-LOX capacity IC50 values 8.635 10.851 respectively. revealed high bind active sites enzymes crucially involved process. analysis their good absorption, distribution, metabolism profiles, they found be safe well. Study findings suggest promising source for development compounds. This, turn, may facilitate incorporation into cosmetic formulations after further investigation.

Language: Английский

Citations

6
Protective Action Mechanisms of Launaea mucronata Extract and Its Nano-Formulation against Nephrotoxicity in Rats as Revealed via Biochemical, Histopathological, and UPLC-QTOF–MS/MS Analyses DOI Creative Commons

Amany A. El-Fadaly,

Inas Y. Younis, Mohamed F. Abdelhameed

et al.

Metabolites, Journal Year: 2023, Volume and Issue: 13(7), P. 786 - 786

Published: June 23, 2023

Plants belonging to the Launaea genus have been extensively utilized ethnopharmacologically treat a variety of diseases, including kidney disorders. Chromium is common industrial pollutant that has linked disease. The present work was designed for investigation UPLC-QTOF-MS/MS metabolite profile L. mucronate ethanolic extract (LME), along with assessing mechanistic protective actions LME and its nano-silver formulation (LMNS) against K2Cr2O7-induced nephrotoxicity in rats. LMNE successfully biosynthesized confirmed using UV-Visible (UV-Vis) spectroscopy transmission electron microscopy (TEM). nephroprotective effects assessed rats exposed potassium dichromate (K2Cr2O7, 15 mg/kg BW) cause nephrotoxicity. LMNS, separately, were administered twice daily 14 days at doses 200 400 BW, respectively. function, catalase, UGT, Nrf2, PGE2, Cox-2, ERK, MAPK levels renal tissue all assessed, histopathological examinations exploring their ameliorative effects. Forty-five bioactive metabolites annotated flavonoids, phenolic organic acids, coumarins, fatty acids. Metabolite profiling revealed chlorogenic acid, apigenin, luteolin glycosides main phenolics, acid-O-hexoside reported first time LME. findings serum function indicators (urea creatinine) markedly elevated K2Cr2O7-intoxicated Furthermore, inflammatory (COX-2 PGE2), MAPK, ERK tissue, whereas Nrf2 downregulated. Histological immunohistochemical assays toxic K2Cr2O7 kidneys. In contrast, administration LMNS prior considerably improved architecture while also restoring most biochemical markers. Functioning via inhibition MAPK/ERK pathway, activating modifying antioxidant metabolic enzymes, exerted toxicity.

Language: Английский

Citations

13
GC-MS analysis and the effect of topical application of essential oils of Pinus canariensis C.Sm., Cupressus lusitanica Mill. and Cupressus arizonica Greene aerial parts in Imiquimod–Induced Psoriasis in Mice DOI
Rania M. Kamal, Manal M. Sabry, Ali M. El-Halawany

et al.

Journal of Ethnopharmacology, Journal Year: 2023, Volume and Issue: 318, P. 116947 - 116947

Published: July 21, 2023

Language: Английский

Citations

11
Exploring geographic variations in quinoa grains: Unveiling anti-Alzheimer activity via GC–MS, LC-QTOF-MS/MS, molecular networking, and chemometric analysis DOI Creative Commons

Inas Y. Younis,

Mohamed S. Sedeek,

Ahmed F. Essa

et al.

Food Chemistry, Journal Year: 2024, Volume and Issue: 465, P. 141918 - 141918

Published: Nov. 5, 2024

Language: Английский

Citations

4
Durable Bio-Based Hydrophobic Recrystallized Wax Coatings DOI

Santhra Krishnan P.,

Sriharitha Rowthu, Sreeram K. Kalpathy

et al.

ACS Applied Bio Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 31, 2025

Bio-waxes derived from natural species are beneficial for preparing non-wetting surfaces. Herein, the wetting properties of recrystallized wax coatings extracted three naturally occurring superhydrophobic species-, Lotus leaves, Bauhinia and Periwinkle flowers, reported as a function recrystallization time, temperature, pH water, impact pressure. showcased nanorods similar to that while waxes could not replicate micro-/nanofeatures their respective species. exhibited water contact angles (WCAs) ∼150°, roll-off (RAs) ∼8°, self-cleaning properties. On contrary, both showed WCAs only ∼110°. Nevertheless, all demonstrated remarkable temporal stability over 180 days, retaining hydrophobicity. They also exhibit excellent thermal up 100 °C chemical variations 2.6 11.5. Furthermore, they withstand 3000 droplets without losing All very low moisture absorption coefficients in order (5.5 × 10-4 wt %/day) < (6.75 (1.075 10-3 %/day), making them highly effective resistant applications such food packaging, protective wood finishes, etc.

Language: Английский

Citations

0