LC/MS/MS and GC/MS/MS metabolic profiling of Leontodon hispidulus, in vitro and in silico anticancer activity evaluation targeting hexokinase 2 enzyme

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: March 22, 2024

Abstract Leontodon hispidulus Boiss is a wild annual plant growing in Egypt. The present study aims for the first time, to evaluate phytochemical profile of main secondary metabolites optimized ethanolic extract using Quadrupole Time-of-Flight Liquid chromatography-mass spectrometry and Gas spectrometry. It also assess anticancer activity its different fractions against prostate carcinoma cell line. Moreover, an in-silico docking was performed Hexokinase-two enzyme. LC- q ToF-MS analysis revealed tentative identification 36 phenolic compounds including glycosides (luteolin, quercetin, kaempferol, apigenin, isorhamnetin, daidzein), coumarines (esculin, esculetin, daphnetin), acids (chlorogenic, caffeic, quinic, P -coumaric, rosmarinic). GC–MS/MS presence 18 where palmitic acid, myristic alpha-amyrin, beta-amyrin were major ones. cytotoxic results that methylene chloride ethyl acetate showed highest PC3 line, with IC 50 values 19, 19.6 μg/ml, respectively. Interestingly, demonstrated apigenin-7- O -glucoside, luteolin-7- kaempferol-3- -glucuronide, quercetin-4′- esculin, rosmarinic chlorogenic α-amyrin exhibited high affinity selected target, HEK-2

Language: Английский

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Green synthesis of zinc oxide nanoparticles using ethanolic extract of Gliricidia sepium (Jacq.) Kunth. ex. Walp., stem: Characterizations and their gastroprotective effect on ethanol-induced gastritis in rats

Bioorganic Chemistry, Journal Year: 2024, Volume and Issue: 145, P. 107225 - 107225

Published: Feb. 23, 2024

Language: Английский

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Protective effects of Euphorbia heterophylla against testicular degeneration in streptozotocin-induced diabetic rats in relation to phytochemical profile

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(1), P. e0314781 - e0314781

Published: Jan. 16, 2025

Background Diabetes mellitus (DM) poses a major risk to human health due an array of implications, one which is detrimental effect on the testicular and reproductive functions. Euphorbia heterophylla widely recognized for its medicinal properties worldwide. Methods findings The objective this study was profile E . ethanol extract (EH-EtOH) elucidate protective role in oxidative stress, relieving inflammatory action hyperglycemia-induced degeneration restoring normal histological structure with physiological tissue streptozotocin (STZ)-induced DM. High-resolution ultra-performance liquid chromatography-mass spectrometry (UHPLC-ESI-Orbitrap-MS) analysis employed analyze compounds present EH-EtOH. EH-EtOH against rat model DM evaluated by measuring improvements blood glucose levels, body weight, inflammation, damage, microcirculation impairment, apoptosis cells induced STZ. chemical profiling revealed presence 52 compounds, including phenolic acids, flavonoids, coumarins, phloroglucinols, triterpenes. Notably, identified isovitexin- C -hexoside, isorhamnetin- O diosmetin, halfordin first time species. Treatment effectively mitigated damage caused STZ, as evidenced restored testosterone (T4) levels antioxidant capacity, reduced expression pro-inflammatory cytokines, improved microcirculation, inhibition testes. Conclusions These results emphasize potential therapeutic related male infertility dysfunctions via antioxidant/angiogenetic /anti-apoptotic effect.

Language: Английский

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Citrus clementine Peel Essential Oil Ameliorates Potassium Dichromate-Induced Lung Injury: Insights into the PI3K/AKT Pathway

Metabolites, Journal Year: 2024, Volume and Issue: 14(1), P. 68 - 68

Published: Jan. 19, 2024

Acute Lung Injury (ALI) is a life-threatening syndrome that has been identified as potential complication of COVID-19. There critical need to shed light on the underlying mechanistic pathways and explore novel therapeutic strategies. This study aimed examine effects Citrus clementine essential oil (CCEO) in treating potassium dichromate (PDC)-induced ALI. The chemical profile CCEO was created through GC–MS analysis. An vivo rats conducted evaluate effect administrated via two different delivery systems (oral/inhalation) mitigating acute lung injury induced by intranasal instillation PDC. Eight volatile compounds were identified, with monoterpene hydrocarbons accounting for 97.03% constituents, including 88.84% D-limonene. at doses 100 200 mg/kg bw exhibited antioxidant anti-inflammatory properties. These significant properties revealed reduction malondialdehyde (MDA) restoration reduced glutathione (GSH). In addition, inflammation observed decreasing levels cytokines tumor necrosis factor-α growth factor-β (TNF-α TGF-β), along an increase phosphatidylinositide-3-kinase (PI3K) Akt overexpression tissue homogenate, both oral inhalation routes, compared PDC-induced group. results supported histopathological studies immunohistochemical assessment TGF-β tissues. findings plays integral role relieving ALI PDC suggests it promising remedy.

Language: Английский

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Enhancement of Female Rat Fertility via Ethanolic Extract from Nigella sativa L. (Black Cumin) Seeds Assessed via HPLC-ESI-MS/MS and Molecular Docking

Molecules, Journal Year: 2024, Volume and Issue: 29(3), P. 735 - 735

Published: Feb. 5, 2024

The characteristic chemical composition of Nigella seeds is directly linked to their beneficial properties. This study aimed investigate the phytochemical sativa using a 100% ethanolic extract HPLC-ESI-MS/MS. Additionally, it explored potential biological effects on female rat reproduction. Follicle Stimulating Hormone (FSH), Luteinizing (LH), Estrogen (E2), and Progesterone (P4) hormone levels were also assessed, along with morphological histological ovarian, oviductal, uterine tissues. Molecular docking was performed understand extract’s activity its role in regulating reproduction by assessing binding affinity hormonal receptors. Twenty metabolites, including alkaloids, saponins, terpenes, flavonoids, phenolic acids, fatty found N. through HPLC-ESI-MS/MS study. seed exhibited strong estrogenic LH-like activities (p < 0.05) weak FSH-like activity. Furthermore, increased serum LH 0.05), P4 hormones 0.001), E2 0.0001). results displayed interaction Erβ, LH, GnRH, receptors, respectively. Based these findings, demonstrated hormone-like activities, suggesting as treatment for improving fertility.

Language: Английский

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Phytopharmaceutical and innovative nanocarriers for chronic kidney diseases: a comprehensive review

Discover Plants., Journal Year: 2025, Volume and Issue: 2(1)

Published: Jan. 28, 2025

Chronic kidney disease (CKD) is a growing global health issue, marked by high mortality rate and significant cardiovascular risks. Despite advancements in treatment, definitive cure remains out of reach, as current approaches mainly focus on managing underlying conditions. The progression CKD largely influenced oxidative stress, fibrosis, inflammation, which present the key areas for therapeutic intervention. Natural therapies encompassing plant extracts bioactive molecules have been promising nephroprotective action due to their anti-inflammatory, diuretic, antioxidative, anti-fibrotic properties. immense exploration availability drugs management, hurdles remain formulation development active drug delivery these phytoconstituents. A thoughtful examination properties phytoconstituents selection developing them ideal dosage forms can reduce gap phytopharmaceuticals. Consequently, loaded nanocarriers hold promise safe effective at site produce desired outcomes. Incorporating natural compounds into nanotechnology-based systems could revolutionize treatment approaches. Additionally, understanding biochemical pathways offers opportunities renal-specific targeting optimized use review examines potential utilization phytopharmaceuticals suitable occurrence mechanisms, that be considered delivery.

Language: Английский

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Anticryptosporidial action mechanisms of Launaea spinosa extracts in Cryptosporidium parvum experimentally infected mice in relation to its UHPLC-MS metabolite profile and biochemometric tools

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(3), P. e0317497 - e0317497

Published: March 3, 2025

Cryptosporidium parvum, a leading cause of diarrhea, is responsible for millions food and waterborne illnesses in humans animals worldwide. Launaea spinosa (Asteraceae family) common herb found the desert Mediterranean region, encompassing peninsula Sinai. Traditionally, it has been utilized managing gastrointestinal issues inflammation. The present study aimed to assess (LS) extracts viz. ethyl acetate (LS-EtOAc), ethanol (LS-EtOH), n-butanol (LS-BuOH), different polarities against C. parvum experimentally infected mice based on immunological, biochemical, histo- immunohistochemical assays. Extracts were characterized via UHPLC-ESI-LIT-Orbitrap-MS metabolite profiles subjected correlation modeling with bioactivities supervised Partial Least Square (PLS) identify active agents. Most L. reduced fecal oocyst count mucosal burden (P < 0.05) than untreated mice, LS-BuOH (200 mg/kg) exerting highest reduction percentage (97%). These increased immunoglobulin G (IgG) levels treated at all examined days post treatment. Also, Interferon-Gamma (IFN-γ) Interleukin-15 (IL-15) obtained after 10 inoculation (dPI), which restored healthy state 21 days, concurrent decrease Tumor Necrosis Factor-Alpha (TNF-α) 0.001). liver enzyme (alanine aminotransferase, aspartate alkaline phosphatase) infection likewise extract administration. LS caused significant increase antioxidant glutathione peroxidase (GSH-Px) catalase Examination colon tissue revealed that infected-treated exhibited expression cleaved caspase-3, damage score, degenerative changes. Metabolite profiling led identification 86 components, primarily phenolic acids, flavonoids, triterpenoid saponins, fatty first report sulfated saponins genus. PLS regression analysis bioeffects significantly positioned close (R2: 0.9) mostly attributed flavonoid glycosides. This demonstrated potential anti-cryptosporidial effects extracts, especially LS-BuOH, suggesting its inclusion future nutraceuticals

Language: Английский

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Phytochemical profiling of Pelargonium graveolens l’Hér and evaluation of its activity against D-galactose induced skin ageing

Natural Product Research, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 13

Published: April 5, 2025

Pelargonium graveolens L'Hér is an evergreen small shrub with pink flowers. Metabolite profiling of its extract by UHPLC/MS analysis revealed the identification 51 secondary metabolites. Interestingly, 29 compounds identified for first time. The colorimetric assays high content phenolic, flavonoids and tannins (100.19 ± 3.94 µg Gallic acid Eq/mg, 47.38 2.04 Rutin Eq/mg 332.75 8.85 Catechin extract) respectively along good antioxidant activity. Moreover, in vitro showed a significant anti-hyaluronidase activity (IC50 SD = 15.75 0.8), as compared to standard luteolin. Topical application characterised P. hydrogel (1% 2% w/w) was tested using D-galactose-induced ageing model mice. Histopathological examination demonstrated increase dermal collagen expression, alleviation ageing-related oxidative stress, stabilisation MMP-1/TIMP-1 balance. Ultimately, could be valuable source natural antioxidants promising antiaging

Language: Английский

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Preparation and characterization of Esculetin loaded nanostructured lipid carriers gels for topical treatment of UV-induced psoriasis

Pharmaceutical Development and Technology, Journal Year: 2024, Volume and Issue: 29(8), P. 886 - 898

Published: Sept. 13, 2024

As an inflammatory and autoimmune skin condition, psoriasis affects 2-3% of people worldwide. Psoriasis requires prolonged treatments with immunosuppressive medications which have severe adverse effects. Esculetin (Esc) is a natural medication that has been utilised to treat psoriasis.

Language: Английский

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Alleviating effect of chlorogenic acid on oxidative damage caused by hydrogen peroxide in bovine intestinal epithelial cells

Journal of Veterinary Medical Science, Journal Year: 2024, Volume and Issue: 86(9), P. 1016 - 1026

Published: Jan. 1, 2024

Chlorogenic acid (CGA) is a polyphenol substance contained in many plants, which has good antioxidant activity. This experiment aimed to explore the protective effects of CGA on hydrogen peroxide(H2O2)-induced inflammatory response, apoptosis, and capacity bovine intestinal epithelial cells (BIECs-21) under oxidative stress its mechanism. The results showed that compared with treated H2O2 alone, pretreatment could improve viability BIECs-21. Importantly, significantly reduced formation malondialdehyde (MDA), lowered reactive oxygen species (ROS) levels, enhanced activities superoxide dismutase (SOD) glutathione peroxidase (GSH-PX) (P<0.05). In addition, can also barrier by increasing abundance tight junction proteins claudin-1 occuludin. Meanwhile, reduce gene expression levels pro-inflammatory factors Interleukin-6 (IL-6) Interleukin-8 (IL-8), increase anti-inflammatory factor Interleukin-10 (IL-10), promote nuclear factor-related 2 (Nrf2) signaling pathway, enhance cell capacity, inhibit Nuclear Factor Kappa B (NF-κB) activation pathway reducing thereby alleviating inflammation damage.

Language: Английский

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