Lipopolysaccharide-induced lung cell inflammation and apoptosis are enhanced by circ_0003420/miR-424-5p/TLR4 axis via inactivating the NF-κB signaling pathway DOI

Hailing Yang,

Chunmei Zhang, Zhongyan Zhao

et al.

Transplant Immunology, Journal Year: 2022, Volume and Issue: 74, P. 101639 - 101639

Published: June 3, 2022

Language: Английский

Exosomes Derived from Apelin-Pretreated Mesenchymal Stem Cells Ameliorate Sepsis-Induced Myocardial Dysfunction by Alleviating Cardiomyocyte Pyroptosis via Delivery of miR-34a-5p DOI Creative Commons

Ting Li,

Yuechu Zhao,

Zhi Cao

et al.

International Journal of Nanomedicine, Journal Year: 2025, Volume and Issue: Volume 20, P. 687 - 703

Published: Jan. 1, 2025

Background: Exosomes sourced from mesenchymal stem cells (MSC-EXOs) have become a promising therapeutic tool for sepsisinduced myocardial dysfunction (SMD).Our previous study demonstrated that Apelin pretreatment enhanced the benefit of MSCs in infarction by improving their paracrine effects.This aimed to determine whether EXOs Apelin-pretreated (Apelin-MSC-EXOs) would potent cardioprotective effects against SMD and elucidate underlying mechanisms.Methods: MSC-EXOs Apelin-MSC-EXOs were isolated identified.Mice neonatal cardiomyocytes (NCMs) treated with or under lipopolysaccharide (LPS) condition vitro.Cardiomyocyte pyroptosis was determined TUNEL staining.RNA sequencing used identify differentially expressed functional miRNAs between Apelin-MSC-EXOs.MSC-EXOs transplanted into mouse model induced cecal ligation puncture (CLP) via tail vein.Heart function evaluated echocardiography.Results: Compared MSC-EXOs, Apelin-MSC-EXO transplantation greatly cardiac mice.Both suppressed cardiomyocyte vivo vitro, latter exhibiting superior protective effects.miR-34a-5p effectively mediated exert high mobility group box-1 (HMGB1) as potential target.Mechanistically, delivered miR-34a-5p injured cardiomyocytes, thereby ameliorating regulation HMGB1/AMPK axis.These partially abrogated downregulation Apelin-MSC-EXOs.Conclusion: Our revealed key component protected mediation signaling pathway.

Language: Английский

Citations

0
RBM3 is associated with acute lung injury in septic mice and patients via the NF-κB/NLRP3 pathway DOI

Feiyu Long,

Liren Hu,

Yingxu Chen

et al.

Inflammation Research, Journal Year: 2023, Volume and Issue: 72(4), P. 731 - 744

Published: Feb. 13, 2023

Language: Английский

Citations

9
METTL3-m6A methylation inhibits the proliferation and viability of type II alveolar epithelial cells in acute lung injury by enhancing the stability and translation efficiency of Pten mRNA DOI Creative Commons
Qiuyun Wang, Jie Shen,

Shiyuan Luo

et al.

Respiratory Research, Journal Year: 2024, Volume and Issue: 25(1)

Published: July 15, 2024

Abstract Background The pathogenesis of acute lung injury (ALI) involves a severe inflammatory response, leading to significant morbidity and mortality. N6-methylation adenosine (m6A), an abundant mRNA nucleotide modification, plays crucial role in regulating metabolism function. However, the precise impact m6A modifications on progression ALI remains elusive. Methods models were induced by either intraperitoneal injection lipopolysaccharide (LPS) into C57BL/6 mice or LPS-treated alveolar type II epithelial cells (AECII) vitro. viability proliferation AECII assessed using CCK-8 EdU assays. whole-body plethysmography was used record general respiratory functions. M6A RNA methylation level after LPS insults detected, then “writer” screened. Afterwards, we successfully identified targets that underwent mediated METTL3, methyltransferase-like enzyme. Last, evaluated regulatory METTL3-medited at phosphatase tensin homolog ( Pten ) ALI, assessing proliferation, inflammation AECII. Results marked damages functions cellular injuries modification expression methyltransferase, exhibited notable rise both tissues cultured subjected treatment. METTL3 knockdown inhibition improved AECII, also reduced level. In addition, stability translation enhanced METTL3-mediated over-expression PTEN reversed protective effect Conclusions can be attributed elevated levels as it promotes through modification. This suggests targeting could offer novel approach for treating ALI.

Language: Английский

Citations

3
Phillyrin prevents sepsis-induced acute lung injury through inhibiting the NLRP3/caspase-1/GSDMD-dependent pyroptosis signaling pathway DOI Creative Commons
Ji Chen,

Xiaoyan Hao,

Zhiyi Li

et al.

Acta Biochimica et Biophysica Sinica, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 6, 2024

Acute lung injury (ALI) is a severe pulmonary disorder of sepsis with high clinical incidence and mortality. Nucleotide-binding oligomerization domain-like receptor family pyrin domain containing 3 (NLRP3)-cysteinyl aspartate specific proteinase 1-gasdermin D (GSDMD)-dependent pyroptosis alveolar epithelial cells (AECs) has emerged as crucial contributor to ALI during sepsis. Phillyrin (PHI), natural lignan isolated from the traditional Chinese herbal medicine

Language: Английский

Citations

3
Shenhuangdan decoction alleviates sepsis-induced lung injury through inhibition of GSDMD-mediated pyroptosis DOI

Ya‐Hui Hu,

Shasha He, Xiaolong Xu

et al.

Journal of Ethnopharmacology, Journal Year: 2023, Volume and Issue: 318, P. 117047 - 117047

Published: Aug. 14, 2023

Language: Английский

Citations

9
Glycyrrhizic Acid Attenuates the Inflammatory Response After Spinal Cord Injury by Inhibiting High Mobility Group Box-1 Protein Through the p38/Jun N-Terminal Kinase Signaling Pathway DOI

Zhiwu Wu,

Zhihua Wang, Zhiping Xie

et al.

World Neurosurgery, Journal Year: 2021, Volume and Issue: 158, P. e856 - e864

Published: Nov. 24, 2021

Language: Английский

Citations

18
Research on the interaction of astragaloside IV and calycosin in Astragalus membranaceus with HMGB1 DOI Creative Commons

Junyi Ye,

Yong Huang,

Xuewa Jiang

et al.

Chinese Medicine, Journal Year: 2023, Volume and Issue: 18(1)

Published: July 4, 2023

High mobility group box 1 protein (HMGB1), a lethal late inflammatory mediator, contributes to the pathogenesis of diverse and infectious diseases. Astragaloside IV calycosin as active ingredients in Astragalus membranaceus, possess potent regulatory ability on HMGB1-induced inflammation, however, interaction between these two phytochemicals HMGB1 has not been elucidated yet.To further investigate astragaloside IV, with protein, surface plasma resonance (SPR) series spectroscopic methods, including UV spectra, fluorescence spectroscopy, circular dichroism (CD), were used. Molecular docking was also carried out predict atomic level's binding modes components HMGB1.Astragaloside found be able bind directly affect secondary structure environment chromogenic amino acids different extents. In silico, showed synergistic effect by independent domains B-box A-box HMGB1, respectively, where hydrogen hydrophobicity bonds regarded crucial forces.These findings that impaired its proinflammatory cytokines function, providing new perspective for understanding mechanism A. membranaceus treating aseptic

Language: Английский

Citations

7
Calycosin prevents IL-1β-induced articular chondrocyte damage in osteoarthritis through regulating the PI3K/AKT/FoxO1 pathway DOI
Xiang Guo, Xiaoyu Pan, Jian Wu

et al.

In Vitro Cellular & Developmental Biology - Animal, Journal Year: 2022, Volume and Issue: 58(6), P. 491 - 502

Published: June 1, 2022

Language: Английский

Citations

9
Acteoside alleviates blood–brain barrier damage induced by ischemic stroke through inhibiting microglia HMGB1/TLR4/NLRP3 signaling DOI

Yucheng Liao,

Junping Hu, Chao Guo

et al.

Biochemical Pharmacology, Journal Year: 2023, Volume and Issue: 220, P. 115968 - 115968

Published: Dec. 15, 2023

Language: Английский

Citations

5
Role of Danggui Buxue Decoction for the prevention and treatment of cardiovascular and pulmonary diseases. DOI

Lin Jiang,

Liping Chen, Wenbin Li

et al.

PubMed, Journal Year: 2023, Volume and Issue: 48(10), P. 1479 - 1493

Published: Oct. 28, 2023

Cardiovascular and pulmonary diseases have become the major disease endangering people's physical mental health because of its high morbidity mortality. Danggui Buxue Decoction, a noteworthy classical prescription, is composed astragali radix angelicae sinensis at ratio 5꞉1. It prescription for tonifying both vital energy blood has effect dredging collaterals. Its main active components, such as polysaccharides, saponins, flavonoids volatile oils, good effects on antioxidation, inflammation regulation, immune regulation promotion circulation. Decoction can improve myocardial ischemia-reperfusion injury heart failure, relieve symptoms diabetic cardiomyopathy, inhibit fibrosis, protect lung fight against cancer other cardiopulmonary diseases. Preclinical studies showed that this oxidative stress, regulate autophagy enhance function through multi-target pathways. Reviewing effective pharmacological action mechanism, clinical application are helpful to provide comprehensive information in prevention treatment cardiovascular

Language: Английский

Citations

4